Cortical and medullary lipids of normal and nephrosclerotic human kidney

• 1.1. Major lipid classes from cortical and medullary zones of normal and nephrosclerotic human kidneys have been isolated and the fatty acid composition of each determined.
• 2.2. The nephrosclerotic tissue contained two times more total lipid than the normal kidney but, irrespective of kidney pathology, phospholipids were the major cortical lipids and neutral lipids were the predominant lipids in medullary zones.
• 3.3. Human kidney contained large amounts of phosphatidylcholines, phosphatidylethanolamines and sphingomyelins and although these were slightly increased in the sclerotic kidney, anatomical differences in phospholipid content were not significant.
• 4.4. Quantitative differences between the zones of normal kidney were found with triglycerides, diglycerides. free fatty acids and cholesterol; overall, the sclerotic tissue contained more triglycerides and small amounts of cholesterol esters with less significant regional differences.
• 5.5. Palmitic, oleic and stearic acid were the major fatty acids of neutral lipids; these plus linoleic acid were prevalent in phospholipids.

     

A comparison of aspartate transcarbamylase activity in juvenile pacific oysters (Crassostrea gigas) when fed various diets

• 1.1. The optimum pH for measurement of aspartate transcarbamylase activity in oyster tissue was determined to be 9.35 while the optimum temperature was 39.5°C.
• 2.2. Aspartate transcarbamylase activity varied significantly over short periods of time (hr) possibly due to fluctuations in the amount of food digested.
• 3.3. The composition of the oyster's diet also affected the levels of aspartate transcarbamylase activity in oyster tissues.
• 4.4. Those oysters fed an egg yolk-starch diet contained significantly lower aspartate transcarbamylase activity than oysters fed an egg yolk-starch-salmon oil diet or a casein-starch-salmon oil diet.
• 5.5. The aspartate transcarbamylase activities in oysters fed Phacedactylum tricornutum or a starch diet were not significantly different from the activities in oysters fed the egg yolk-starch diet.

     

Changes of the fatty acid composition in smolts of masu salmon (Oncorhynchus masou), associated with desmoltification and sea-water transfer

• 1.1. Tissue lipid compositions of desmoltified yearlings of masu salmon (Oncorhynchus masou) obtained by keeping smoltified fish in fresh water, were examined and compared to those of smoltified fish before and after transfer to sea-water (SW).
• 2.2. Lipid contents of muscle, liver, gut and gills of desmolts tended to increase compared to those of initial smolts.
• 3.3. The increased proportion of triacylglycerol (TG) and decreased proportion of phospholipids (PL) characterized the tissue lipids of desmolts.
• 4.4. Liver and muscle lipids showed no distinct differences both in content and proportion between initial and SW smolts, but gut and gill lipids of SW smolts decreased in content accompanied by a decrease of TG and an increase of PL in proportion.
• 5.5. Excepting muscle non-polar lipids, tissue lipids of desmolts contained more mono-unsaturated fatty acids and saturated fatty acids and less polyunsaturated fatty acids (PUFA), especially (n-3) PUFA such as 22:6(n-3), than those of initial and SW smolts.
• 6.6. No large differences in fatty acid composition were seen between initial and SW smolts except for the gut.
• 7.7. The proportion of (n-3) PUFA in the gut of SW smolts was higher than that of initial smolts.
• 8.8. The results indicated that masu salmon smolts can modify their lipid metabolism to adapt to ambient salinity changes. The proportion of (n-3) PUFA particularly in polar lipids, or in osmoregulatory organs such as gut and gills, was seen to be critical in lipid types of freshwater- or sea-water-adapted fish.

     

Utilization of body stores in embryonated ova and larvae of two coregonid species (Coregonus lavaretus L. and C. albula L.)

• 1.1. Developing eggs of whitefish (Coregonus lavaretus L.) and vendace (Coregonus albula L.) were kept at 1–2°C and some eggs taken gradually up to 8°C to provoke mass hatching of embryos.
• 2.2. Wet weight, dry matter and the contents of lipid, protein and ash were measured in fish during the course of experiment.
• 3.3. Dry matter content decreased gradually in whitefish eggs from 15.64 to 11.95% during 1 month at 1–2°C, whereas vendace eggs showed only a slight decrease from 16.27 to 15.53%.
• 4.4. In both species protein content decreased but lipid increased when approaching the natural time of hatching.
• 5.5. During delayed hatching at low water temperatures protein contributes to catabolism, whereas lipid content decreased only in the later phase of the experiment.
• 6.6. Larvae starved for 10 days after hatching lost increasing amounts of dry matter (from 26.1 to 50.3% of body weight) and protein (from 18.7 to 45.9% of body weight) as they remained longer in cold water as embryos.
• 7.7. A correspondence was found between assessment of metabolic utilization of body stores based on chemical analysis of fish body and previous work on oxygen consumption and nitrogen excretion.

     

Biochemical aspects of Balanus hameri haemolymph,together with some comparative haemolymph data for Balanus balanus and Lepas anatifera

• 1.1. The concentration of protein in the haemolymph of Balanus hameri ranged from 2.0 to 17.3 mg/ml, and the lipid from 1.4 to 7.7 mg/ml; the haemolymph protein and lipid levels increased significantly prior to cross-fertilization.
• 2.2. The protein and lipid concentrations in Balanus balanus haemolymph were 8.1 and 1.7 mg/ml respectively.
• 3.3. The lipid concentration of Lepas anatifera haemolymph was 1.2 mg/ml.
• 4.4. The neutral lipid and phospholipid components of B. hameri and L. anatifera haemolymph were the same, with the major components of the phospholipid fraction being phosphatidyl ethanolamine and phosphatidyl choline.
• 5.5. The osmolarity (970.4 mOsm), chloride ion concentration (501.3 m-eq/l) and pH (7.29) of B. hameri haemolymph were also determined.

     

Seasonal changes in condition and lipids composition of the bivalve Macoma balthica L. from the gulf of Gdańsk (Southern Baltic)

• 1.1. The total lipids content and composition of lipid classes, their per cent in dry wt of soft tissues and level in standard animal, as well as composition of fatty acids and sterols were studied in Macoma balthica collected from three sites in the Gulf of Gdańsk, in the years, 1983–1984.
• 2.2. The increase in the content of total lipids, triacylglycerols, oleopalmitic, 16:1 and eicosapentaenoic acids, 20:5, C27 sterols (mainly cholest-5en-3β-ol), in spring and early summer and their decrease in autumn and winter were observed.
• 3.3. Content of phospholipids, sterols and hydrocarbons in the tissue dry wt of Macoma balthica remained nearly constant.

     

Comparative study on the fatty acid and lipid composition of four marine fish larvae

• 1.1. Fatty acid and lipid class composition were determined in larvae of four marine species: Atlantic halibut (Hippoglossus hippoglossus L.), plaice (Pleuronectes platessa), cod (Gadus morhua) and turbot (Scophthalmus maximus) at hatching and prior to first feeding.
• 2.2. Total fatty acid content decreased in the four species with up to 50% reduction in one of the halibut groups. Docosahexanaoic acid (22:6 n-3) was especially utilized.
• 3.3. Low lipid utilization was found in turbot in relation to the other three species.
• 4.4. Water environmental temperature may explain some of the differences in the fatty acid utilization and the source of metabolic energy between cold water species (halibut, cod, and plaice) and temperate species (turbot), in the period from hatching to prior to first feeding.
• 5.5. Relative amounts of neutral lipids and phospholipids were similar in plaice, cod and halibut, approximately 25% and 75% of total lipids, respectively, and were approximately constant during the yolk-sac stage. Neutral lipids were dominant for turbot at hatching, accounting for 53–55% of the total lipids, while phospholipids predominated prior to first feeding, being 56–59%.
• 6.6. Phosphatidylcholine was catabolized in halibut, plaice and cod but not in turbot, while phosphatidylethanolamine tended to be synthesized in all four species.

     

Purification and characterization of lipoprotein lipase from the white adipose,skeletal muscle,cardiac muscle,mammary gland and lung tissues of the rat

• 1.1. Lipoprotein lipase (LPL) was isolated from five rat tissues: white adipose, skeletal muscle, cardiac muscle, mammary gland and lung.
• 2.2. Specific activity of the preparations varied from 75 U/mg for skeletal muscle and 720 U/mg for adipose.
• 3.3. The preparations were further analysed using SDS-PAGE and a single component identified. The mol. wt of 61,000 Da of this component was consistent for all five of the tissue sources.
• 4.4. Significant differences in the values of the isoelectric points of the enzyme species were revealed. The values varied from 7.23 (SEM 0.022) for cardiac and lung to 7.51 (SEM 0.037) for mammary.
• 5.5. Two-dimensional electrophoresis, using isoelectric focusing in the first dimension and SDS-PAGE in the second revealed differences in the patterns of stained material derived from the five tissue sources.

     

The influence of ethanol on lipid metabolism in mouse tissues

• 1.1. The double isotope ratios and specific radioactivities of individual lipid fractions in major tissues of mice have been determined before and after ethanol ingestion.
• 2.2. Several significant alterations in these ratios were caused by this treatment, with marked increases in most liver lipids and diverse responses in other tissues.
• 3.3. These data establish that ethanol ingestion causes widespread perturbations in tissue lipid metabolism, with the main emphasis being directed towards an increase in the rate of synthesis of the liver lipid classes.
• 4.4. The data also provides indications of an increase in the peroxisomal oxidation of fatty acids following ethanol ingestion, with the different distribution of ethanol metabolizing systems giving rise to individual metabolic responses in the separate tissues.
• 5.5. These results have been discussed in relation to the eatablished involvements of lipid metabolism and tissue interactions in the whole animal.

     

PCB effects on production of carbohydrates,lipids and proteins in marine diatom Phaeodactylum tricornutum

• 1.1. Aroclor 1254 had an influence on biochemical composition of phytoplankton Phaeodactylum tricornutum.
• 2.2. Total lipid content of Arocolor 1254 treated phytoplankton ranged from 90 to 40%, total carbohydrates from 5 to 50%, while protein content was almost unchanged.
• 3.3. The observed rapid and significant increase in lipid content (150%) within the first 24 hr could be ascribed to their protective role.

     

Seasonal changes of total lipids in the turtle Sternotherus odoratus

• 1.1. Seasonal variation in total lipids was examined in several body components of the turtle Sternotherus odoratus.
• 2.2. Carcass fat stores in both sexes were depleted during winter. Additionally, a decline in carcass lipids was associated with increases in gonadal mass.
• 3.3. Concentrations of liver lipids were maximal during August and minimal during winter.
• 4.4. Males showed little seasonal change in plasma lipid levels, whereas females had seasonal peaks temporally associated with ovarian development and carcass fat storage.
• 5.5. Ovarian concentrations of lipids were minimal after nesting and increased during fall.
• 6.6. Results suggest that S. odoratus uses stored fats both for reproduction and maintenance during winter.

     

Lipid biosynthesis in amphibian oocyte and embryonic cell-free preparations

• 1.1. The utilization of [2-³H]glycerol-3-phosphate in the synthesis of lipids during early embryogenesis was studied in cell-free preparations from oocytes or embryos of Bufo arenarum Hensel
• 2.2. The precursor was incorporated in all stages of development up to gill circulation, which indicates that oocytes and embryos have the enzymatic machinery necessary to synthesize at least part of their own lipids.
• 3.3. A significant decrease in the labeling of most lipids took place after fertilization, especially in gastrulas, but at gill circulation lipid synthesis was highly stimulated.
• 4.4. The incorporation pattern is similar in unfertilized oocyte, fertilized oocyte and gastrulas, where phosphatidylglycerol has the highest amount of radioactivity. At gill circulation stage phosphatidylethanolamine and neutral lipid biosynthesis also became significant.
• 5.5. The results suggest a different regulation of the biosynthetic lipid routes through the appearance of new enzymes or modulators of preexisting enzymes during amphibian development.

     

Effect of triton X-100 treatment on the lipid composition,biochemical and functional properties of hake (Merluccius hubbsi) myofibrils purified from pre- and post-spawned fish

• 1.1. The effects of Triton X-100 treatment on the lipid contents and functional properties of hake myofibrils from pre- and post-spawned fish were investigated.
• 2.2. Differences in lipids, biochemical and functional properties of hake myofibrils related to the gonadal condition of fish were observed.
• 3.3. Triton X-100 treatment removed 65% of polar lipids in myofibrils from pre-spawned fish and only 10% in myofibrils from post-spawned fish.
• 4.4. Triton X-100 increased the Hill coefficient to 1.5 in an allosteric type of reaction for the myofibrillar Mg²⁺-ATPase from pre-spawned hake.
• 5.5. The detergent effect observed on the contraction response was greater in myofibrils from prespawned fish than in post-spawned fish.

     

Atrial natriuretic peptides in the heart and hemolymph of the oyster,Crassostrea virginica: A comparison with vertebrates

• 1.1. The content of atrial natriuretic peptides (ANPs) in the auricles of oysters, Crassostrea virginica, was significantly (P < 0.01) greater than in their ventricles.
• 2.2. High-performance gel permeation chromatography (HP-GPC) followed by ANF radioimmunoassay revealed two peaks in both oyster and vertebrate (rat) hearts—a major peak where the 12.6–14 kDa ANF prohormone elutes and a smaller peak where the pure human form of ANF elutes.
• 3.3. HP-GPC evaluation followed by proANF 31–67 radioimmunoassay revealed only an ANF-like prohormone while HP-GPC followed by proANF 1–30 radioimmunoassay revealed the ANF prohormone and a proANF 1–30-like peptide in oyster and rat hearts.
• 4.4. ANPs concentrations in hemolymph were 940 ± 129, 225 ± 25, and 100 ± 10 pg/ml by the proANF 1–30, proANF 31–67, and ANF radioimmunoassays, respectively.
• 5.5. Atrial natriuretic-like peptides are present in the oyster heart in molecular species similar to vertebrate species and these peptides are also present in hemolymph.

     

Lipid components and utilization in consumers of a seagrass community: An indication of carbon source

• 1.1. The lipid components of three animals, the rock crab Nectocarcinus integrifons, the rock flathead Platycephalus laevigatus and the southern garfish Hyporhamphus melanochir, feeding in the seagrass beds at Corner Inlet, Victoria, Australia have been examined in detail in order to provide further information on seagrass community structure.
• 2.2. Biological marker compounds detected within animal gut content material were used to recognize dietary sources and then utilized by community members.
• 3.3. Both H. melanochir and N. integrifons have been shown to ingest and to varying degrees incorporate seagrass lipid material, thus further confirming the importance of seagrass carbon in the Corner Inlet environment.
• 4.4. The southern sea garfish H. melanochir is observed to remove C₁₈ PUFAs (polyunsaturated fatty acids) from ingested seagrass material.
• 5.5. Seagrass sterols are altered during incorporation into the lipids of this fish.
• 6.6. Lipid-rich digestive juices play a role in the digestive processes of all three animals.
• 7.7. Components tentatively identified as (NMI) (non-methylene interrupted) fatty acids have been detected in the lipids of the garfish H. melanochir and the crab N. integrifons.
• 8.8. The fecal material of all three animals represent possible sources of these lipids (NMI acids) in Corner Inlet sediments.
• 9.9. Based on lipid compositional data, N. integrifons feeds on Posidonia australis detritus and associated epiphyte material.
• 10.10. The removal of both plant and epibiota cellular lipids along the digestive tract of the crab was observed, although structural components such as long chain mono- and α,ω-dicarboxylic acids, which have been previously recognized as seagrass marker lipids are not directly absorbed.

     

Phenoloxidase activity of acridid grasshoppers from the subfamilies melanoplinae and oedipodinae

• 1.1. Phenoloxidase activity and wound melanization was studied in five species of grasshoppers representing the subfamilies Melanoplinae and Oedipodinae.
• 2.2. Most of the phenoloxidase activity was detected in the plasma fraction of grasshopper whole-body homogenates and supernatant fractions of the hemolymph. The species representing the Oedipodinae had 20–50% higher percentage of the total phenoloxidase activity associated with particulate matter from a whole-body homogenate when compared to the Melanoplinae.
• 3.3. Phenoloxidase activity could not be detected in sclerotized cuticle of adult grasshoppers.
• 4.4. The phenoloxidase existed as a zymogen which could be activated by chymotrypsin and inhibited by KCN and NaCN while EDTA showed no effect. It had optimum activity at 37°C and pH 7.3.
• 5.5. These findings are discussed in relation to wound repair and immune responses to infection in grasshopper species.

     

Seasonal variation in the energy metabolism in an estuarine crab,Chasmagnathus granulata (Dana, 1851)

• 1.1. The effects of seasonal variation on the carbohydrate and lipid metabolism of the Chasmagnathus granulata were investigated.
• 2.2. Glycemia is high in winter and summer and low in spring and fall.
• 3.3. The glycogen content in the hepatopancreas and muscle is higher in fall and winter, and decreases during spring and summer.
• 4.4. The muscle lipids are higher in summer, and decrease during fall and winter whereas hepatopancreas lipids are higher except in the fall.
• 5.5. The crabs show change in the metabolic pattern of lipids and carbohydrates during the seasons of the year.

     

The reproduction strategy of oyster ostrea edulis L. from the biochemical point of view

• 1.1. Influence of the biochemical composition of food (four species of micro-algae and one mixture) on the biochemical composition of gonads and larvae of O. edulis (total protein, lipid, carbohydrate, ash content, neutral and polar lipid class composition, amino acid composition and fatty acid composition of total, neutral and polar lipids) and the size of newly released larvae have been investigated.
• 2.2. Precentage of total lipids and triacylglycerols in gonads depends on that in algae (r = 0.52 and 0.69 accordingly).
• 3.3. Gonads rich in lipids had a higher level of triacylglycerols, phospholipids, polar lipids and a lower value of the ratio phosphatidylethanolamine/phosphatidylcholine (PE/PC) than gonads with a low lipid content.
• 4.4. Amino acid composition of gonads depends on that of food, in this case, essential acids are preferentially accumulated (Asp acid, Ser, Ala, Cys, Tyr and Pro) and two non-essential (Thr and Lys).
• 5.5. Fatty acid composition of total lipids of gonads was rather stable; except for the two essential acids 20:523 and 22:6w3, their percentage depends on that of food r = 0.65 and 0.65 accordingly). Fatty acid composition of neutral lipids was more diverse (in number and degree of variety) as compared to polar lipids.
• 6.6. Larvae released from oysters with gonads rich in lipids had a higher percentage of lipids, triacylglycerols, size and a lower ash percentage and value of ratio PE/PC, as compared to larvae from gonads with low lipid content. Total lipid and triacylglycerol contents in gonads correlate rather well with those in larvae (r = 0.77 and 0.47 accordingly).
• 7.7. Phospholipid class composition of larvae strongly depends on that of gonads. All the correlations are high and positive in character (except for phosphatidylinositol).
• 8.8. Amino acid composition of larvae depends on that of gonads and, as in the case with gonads, the same essential acids are accumulated in the first place.
• 9.9. Fatty acid composition of total lipids of newly released larvae was rather stable and independent on that of gonads except for total polyunsaturated acids (r = 0.70) and 20:5w3 (r = 0.65). Fatty acid composition of neutral lipids was lesser diverse (in number and degree of variation) as compared to polar lipids.

     

On the lipochromes in the skin of marine teleost fish with special reference to the painted comber (Serranus scriba L.)

• 1.Yellow and orange chromatophore pigments in the fins and tail of Mugil cephalus, Solea vulgaris and Serranus scriba were extracted with methanol and chloroform and characterized as xanthophylls.
• 2.The xanthophylls, which were responsible for the yellow-orange colour of the tissues, were found to be in a stable association with the particulate matter of tissue homogenates.
• 3.In Serranus scriba about 25 per cent of fin and tail xanthophylls disappeared after 2 days in captivity.
• 4.The injection of cortisol succinate did not affect the concentration of xanthophylls in Serranus.

     

Relationships among serum lipids,milk production and physiological status in dairy cows

• 1.1. Serum concentrations of cholesterol, triglyceride and alpha lipoprotein cholesterol were determined in nine Holstein cows during different stages of their gestation and lactation cycles.
• 2.2. Serum cholesterol concentrations and daily milk yields were significantly correlated during early and late lactation. The correlations were not significant over the entire lactation.
• 3.3. Maximum values for serum cholesterol were observed at mid lactation and minimum at or near parturition. Serum triglyceride concentrations ranged from 4 to 33 mg/dl. Although prepartum levels for triglyceride tended to be higher than postpartum concentrations, no correlation with milk yield was noted.
• 4.4. These data are compared with lipid changes which take place in other mammals during lactation.