Glycolysis and Epilepsy-Induced Changes in Cerebrocortical NAD/NADH Redox State

Book Review in this Article:
Neurology and Neurobiology, Vol. 1: Cytochemical Methods in Neuroanatomy edited by Victoria Chan-Palay and Sanford Palay. Alan R. Liss
: Mechanisms and Functions edited by J. de Belleroche.
Proteins of the Brain and Cerebrospinal Fluid in Health and Disease by Elisabeth Roboz Einstein. Charles C Thomas
The Synthesis of Carbon-11, Fluorine-18, and Nitrogen-13 Labeled Radiotracers for Biomedical Applications by J. S. Fowler and A. P. Wolf.
Thermal Sensations and Thermoreceptors in Man by Herbert Hensel. Charles C Thomas     

The Expression Pattern of NAD(P)H Oxidases and the Cyclic Electron Transport Pathway around Photosystem I of Synechocystis sp. PCC6803 Depend on Growth Conditions

Cyclic electron transport and NADH and/or NADPH (NAD(P)H)-oxidizing activities were investigated in Synechocystis sp. PCC6803 grown under various stressed conditions and in ndhB-less (M55) and ycf33-deletion mutants. Activity staining and inhibitor data suggested that the ferredoxin-quinone reductase (FQR) route is the main pathway in ycf33-deletion and high-light (300 μE m^?2 s^?1)-grown cells as well as in M55 cells. The FQR route was highly sensitive to HgCl₂, but not to diphenyleneiodonium (DPI). On the other hand, cells grown under low CO₂ (0.03%) or normal (100 μE m^?2 s^?1, 3% CO₂) conditions were found perhaps to use the complex I-type NAD(P)H dehydrogenase route, which was found to be highly sensitive to DPI but not to HgCl₂. In high-salt (0.55 M NaCl)-grown cells, the amount of ferredoxin-NADP⁺ oxidoreductase (FNR) increased, and the main cyclic electron flow was perhaps the FNR route. Both DPI and HgCl₂ were strong inhibitors of the FNR route.