Single origin and subsequent diversification of central Andean endemic Umbilicaria species

We studied an Andean endemic group of species of the lichen-forming fungal genus Umbilicaria from the subalpine and low-alpine zone, with their biogeographic center in Bolivia and Peru. A number of species and varieties have been described from this element, but apparent instability in several morphological traits has made it difficult to precisely delimit taxa. Based on DNA sequences of nuclear ITS, LSU and mitochondrial SSU from extensive collections from Argentina, Bolivia, Chile, Colombia, Ecuador and Peru, we present here a molecular phylogenetic analysis of this Andean endemic element within genus Umbilicaria. All analyses (MP, ML and Bayesian) support a single origin for the element and a division into two major groups characterized by different apothecium types: the Umbilicaria dichroa group and U. calvescens group. Taxa U. krempelhuberi, U. peruviana and U. subcalvescens are nested withinn U. calvescens and are treated as conspecific with the latter species. The endemic element shares a most recent common ancestor with the Umbilicaria vellea group, which has a worldwide distribution and contains several asexually reproducing (sorediate) species. Independent reversals to sexual reproduction might explain the evolution of two types of apothecia in this monophyletic endemic lineage. A number of cosmopolitan, mostly high-alpine, species of Umbilicaria also present in the central Andes are related only remotely to the endemic element and do not exhibit speciation into endemics. Because the An-dean element dominates the Umbilicaria habitats of the low- and subalpine zones we propose that the founder colonized the Andes at a time when the mountains had not yet reached their current elevation while the high-alpine species arrived more recently.     

Two new species of Botryosphaeria with brown, 1-septate ascospores and Dothiorella anamorphs

Botryosphaeria sarmentorum sp. nov. and B. iberica sp. nov. are described and illustrated. These two species are unusual in this genus because of their brown, 1-septate ascospores. Phylogenetic analysis based on ITS and EF1-alpha sequences place them within the clade containing species with Fusicoccum anamorphs. The brown, 1-septate conidia, however, do not conform to Fusicoccum. Therefore phylogenetically and morphologically the anamorphs of these two species belong in a genus distinct from any of the currently accepted anamorph genera assigned to Botryosphaeria. Through a study of the type species of Dothiorella this genus is resurrected to accommodate anamorphs of Botryosphaeria with brown, 1-septate conidia. Botryosphaeria sarmentorum is shown to be the teleomorph of Diplodia sarmentorum, which in turn is transferred to Dothiorella. Otthia quercus is transferred to Botryosphaeria as B. quercicola nom. nov.     

New species and nomenclature in Prospodium (Uropyxidaceae, Pucciniales) and the new anamorphic genus Canasta in the neotropics

Ten new teleomorphic species, two status novus taxa and two new combinations in the genus Prospodium are established. The following anamorphs, all closely related to the genus Prospodium, also are proposed: the genus Canasta, its two new species and a new combination, three new combinations for the genus Uredo.     

Two new species ofConiochaeta with a cephalothecoid peridium wall

Two new species ofConiochaeta, isolated from Japanese soils, are described and illustrated:C. cephalothecoides, which is characterized by dark brown ascomata clothed with short setae, cylindrical asci and ovoid to almond-shaped or pyriform ascospores with a longitudinal germ slit; andC. dumosa, which is characterized by dark brown ascomata clothed with short setae and dense hyphal hairs, cylindrical asci and ellipsoid-fusoid ascospores with a longitudinal germ slit. These species are distinguished from most species of the genus by the unique cephalothecoid peridium of their ascomata. The associated anamorphs of both species are assignable to the form-genusLecythophora.     

Molecular phylogeny of Hypoxylon and closely related genera

Phylogenetic relationships were inferred among several xylariaceous genera with Nodulisporium or nodulisporium-like anamorphs based on the analyses of beta-tubulin and alpha-actin sequences. One hundred nine cultures and three specimens of 83 representatives of these four genera were included in the study. Biscogniauxia taxa formed a well supported clade that was basal to the other taxa, while taxa of Hypoxylon and Daldinia comprised a large monophyletic group that contained two subclades. The first subclade encompassed Hypoxylon sect. Annulata and is accepted here as the new genus Annulohypoxylon. The second subclade contained taxa of Hypoxylon sect. Hypoxylon and Daldinia. Hypoxylon is restricted to include only those taxa in sect. Hypoxylon. Thirty-three epithets are made in Annulohypoxylon. Hypoxylon cohaerens var. microsporum is raised to the species level and accepted as A. minutellum. Hypoxylon polyporoideum is recognized as distinct from H. crocopeplum. Hypoxylon placentiforme is accepted as Daldinia placentiformis.     

中国地衣型子囊菌石耳科纲要

该纲要为东亚地衣型子囊菌石耳科研究的一部分.对于该科一些种的子囊盘进行了研究.     

The lichen genera Lasallia and Umbilicaria in East Africa

Three species of Lasallia Mérat and nine species of Umbilicaria Hoffmann are reported from the East African mountains, their vegetative propagules, chemistry, ecology, and distribution are discussed and keys to them are provided. The new combinations Umbilicaria africana (Jatta) Krog & Swinscow, U. soralifera (Frey) Krog & Swinscow, and U. umbilicarioides (B. Stein) Krog & Swinscow are made. Umbilicaria bolusiana Frey, U. haumaniana Frey and U. propagulifera (Vainio) Llano are reduced to synonymy with U. umbilicarioides , and U. glauca var. ruwenzoriensis Frey to synonymy with Lasallia papulosa (Ach.) Llano.     

A new fungal genus, Teracosphaeria, with a phialophora-like anamorph (Sordariomycetes, Ascomycota)

The new genus and species Teracosphaeria petroica is described for a perithecial ascomycete and its anamorph occurring on decayed wood collected in New Zealand. The fungus produces immersed, non-stromatic ceratosphaeria-like perithecia in nature, with hyaline, septate ascospores produced in unitunicate, non-amyloid asci. The anamorph produced in vitro is phialophora-like with lightly pigmented phialides terminating in flaring, deep collarettes that are often noticeably brown with conspicuous periclinal thickening. Phylogenetic analysis of LSU rDNA sequence data indicates that this fungus is distinct from morphologically similar fungi classified in the Chaetosphaeriales, the Trichosphaeriales or the Magnaporthaceae. It forms a monophyletic group with recently described, chaetosphaeria-like ascomycetes, such as the pyrenomycete genus Mirannulata, and shows affinity with the anamorphic species Dictyochaeta cylindrospora. The usefulness of describing anamorph genera for morphologically reduced anamorphs, when anamorph characteristics are actually part of the holomorph diagnosis, is discussed. An apparently contradictory example of the so-called Cordana and Pseudobotrytis anamorphs of Porosphaerella spp. is also discussed.     

The lichen genus <Emphasis Type="Italic">Umbilicaria</Emphasis> from the neighbourhood of Admiralty Bay (King George Island,maritime Antarctic), with a proposed new key to all Antarctic taxa

This paper documents the occurrence of the genus Umbilicaria in the neighbourhood of Admiralty Bay on King George Island in the maritime Antarctic. Nine species are reported from the area surveyed, with Umbilicaria kappeni being reported from King George Island for the first time. The occurrence of U. aprina, U. cristata and U. umbilicarioides on the island is confirmed and that of U. aprina is its first documented record from the maritime Antarctic. Fertile specimens of U. umbilicarioides are recorded for the first time from Antarctica. The area around Admiralty Bay seems to be one of the Antarctic regions with the highest diversity of Umbilicaria species. The diagnostic characters and distribution of each species are discussed. An updated key for identification of all the Antarctic species of the genus is provided.     

Two new Kirschsteiniothelia species with Dendryphiopsis anamorphs cluster in Kirschsteiniotheliaceae fam. nov

Two new Kirschsteiniothelia species are proposed in this study; both were collected on decaying wood from Chiang Mai and Chiang Rai provinces in northern Thailand. The taxa were isolated and the morphological characters are described and illustrated. ITS, LSU and SSU combined sequence analysis showed taxa of Kirschsteiniothelia separating into three lineages: (i) K. elaterascus grouped within Morosphaeriaceae (Pleosporales); (ii) K. maritima clustered with Mytilinidion spp. as a sister group in the Mytilinidiaceae clade; and (iii) the two new Kirschsteiniothelia species, which produce Dendryphiopsis anamorphs in culture, clustered with K. aethiops (the generic type) and the anamorph D. atra. The new family Kirschsteiniotheliaceae is introduced to accommodate taxa grouping with K. aethiops. K. elaterascus is transferred to Morosphaeria (Morosphaeriaceae) and a new genus Halokirschteiniothelia is introduced to accommodate K. maritima (Mytilinidiaceae).     

A New Subgenus of Lasallia Merat Emend. Vej

In the present paper a new species of Lasallia Merat is described and placed under a new subgenus Pleiogyra, characterized by having econcentrical multi-gyrate apothecia. In consequence of this finding, the conception of plane disc in the genus Lasallia of Umbilicariaceae becomes untenable. As a result of careful examination of rich materials, the writer considers the difference in disc found by Scholander among all species of the family is of certain significance for classification. Unfortunately, Scholander’s classification is based solely on this rather variable morphological character. If his view be adopted the new species will have to be inserted into the genus Gyrophora Ach. emend. Schol. If accepting Llano’s division of Umbilicariaceae into five genera, namely Lasallia, Agyrophora, Omphalodiscus, Umbilicaria and Actinogyra, the new species will have to be considered a new genus of the family. The present writer considers both Scholander’s and Llano’s systems as being artificial, because the former author emphasizes too much the unstable morphological appearance of disc and the later simply divides the family into five genera simultaneously on the basis of the stable and unstable characters of the various groups. In contrast with Scholander’s and Llano’s systems Frey’s classification neglects wholly the difference in the characters of disc. Such treatment is likewise unsound. The present writer supports the view held by Flotow, Koerber, Elenkin and Savicz (1950). Nevertheless, he does not agree with Savicz in erecting the subgenus Gyrophoropsis (Elenk. et Savicz) Savicz, because muriform spores are present not only in the group having gyrate discs, but also in the groups having plane and omphalic discs of the Genus Umbilicaria Hoffm. emend. Vej. The recognition of this subgenus must therefore deny the basis on which the other four subgenera are established. The system of Umbilicariaceae held by the writer is as follows: I. Genus Lasallia Merat emend. Vej ...... Thallus pustulate; disc plane to econcentrical multi-gyrate; spores 1(2) per ascus, quite large, muriform. 1. Subgenus Lasallia...Disc plane. 2. Subgenus Pleiogyra Vej ...... Disc econcentrical multigyrate. II. Genus Umbilicaria Hoffm. emend. Vej (syn. Gyrophora Ach. emend. Savicz)* ...Thallus non-pustulate; disc plane and smooth or plane with central sterile column (or fissure) or with gyri; spores 8 per ascus, simple, hyaline or becoming brown muriform, small. 1. Subgenns Agyrophora (Nyl.) Savicz ...... Disc plane. (1) Section Agyrophora (syn. sect. Anthracinae emend. Llano). ..... Spores simple and hyaline. (2) Section Dichroae Llano ...... Spores becoming brown muriform. 2. Subgenus Omphalodiscus (Schol.) Savicz ...... Disc with central sterile column or fissure. (1) Section Omphalodiscus (syn. sect. Decussatae Schol.) ...... Spores simple and hyaline. (2) Section Spodochroae Schol. ..... Spores becoming muriform. 3. Subgenus Umbilicaria...Disc with concentrical gyri; spores simple and hyaline or becoming muriform. (1) Section Umbilicaria (syn. sect. Simplices Llano) ...... Spores simple and hyaline. (2) Section Gyrophoropsis (Elenk. et Savicz) A. Zahlbr. (syn. sect. Muriformes Llano) ...... Spores becoming muriform.4. Subgenus Actinogyra (Schol.) Savicz ...... Disc with radial gyri, proper margin absent.     

New Hypoxylon and Nemania species from Costa Rica and Taiwan

Six xylariaceous fungi, including two Hypoxylon taxa and four Nemania taxa, are described as new. They were collected from either Costa Rica or Taiwan. Two of the Nemania species--N. flavitextura and N. primolutea--were cultured and typical Geniculosporium anamorphs were produced.     

The lichen genus Umbilicaria in Ecuador

Nine species of the saxicolous lichen genus Umbilicaria from Ecuador are reported and a key is provided: U. africana, U. aprina, U. cinereorufescens, U. decussata, U. dendrophora, U. haplocarpa, U. leprosa, U. nylanderiana and U. vellea. The species diversity of this genus on the equatorial high mountains of South America is low compared to North America and Eurasia, but similar to that found on high African peaks close to the equator. The species mostly belong to a high‐alpine element with worldwide distribution. Two species belong to an Andean endemic element, viz U. haplocarpa and U. leprosa. The low diversity and low percentage of endemism may reflect the fairly recent uplift of the Andes and the comparatively small geographic extent of the alpine Andean biota. The dense rainforests of Ecuador leave few suitable open rock habitats for the establishment of these light‐craving lichens below the tree‐line (4000–4200 m a.s.l.), and thus their equatorial habitat is almost exclusively restricted to the alpine zone between the tree‐line and the snow‐line (4700–4800 m a.s.l.). The equatorial species mainly reproduce asexually by thalloconidia, and the adaptive significance of this type of reproduction in high altitude habitats is discussed.     

Ribosomal RNA sequence phylogeny is not congruent with ascospore morphology among species in Ceratocystis sensu stricto

The genus Ceratocystis sensu stricto includes important fungal pathogens of woody and herbaceous plants. This genus is distinguished from species in Ceratocystis sensu lato by the presence of Chalara anamorphs. Ascospore shape has been used extensively in delineating Ceratocystis taxa, which show a large variety of ascospore shapes. Sequence analysis of one region of he 18S ribosomal RNA subunit and two regions of the 28S ribosomal RNA subunit showed that there was a majority of multiple substitutions at nucleotide sites and that there was a low transition/transversion ratio, T = 0.72. Both of these results suggest that these are well established, old species. Ascospore morphology, for the most part, was not congruent with the molecular phylogeny, and the use of morphological characters may be misleading in the taxonomy of these species.      

狭义干蘑属(蘑菇目)概要及新的系统学处理

对狭义干蘑属(Xerulas.str.)的范围进行了重新界定,该属仅包括模式种干蘑(X.pudens)及其周围的几个种。属的特征是担子果金钱菌状,有假根;菌盖及菌柄表面干燥,被有黄色至褐色直立、披针形的刚毛;菌褶边缘无不育带;子实层有近梭形的小担子。这些特征可以将干蘑属的物种从干蘑属-小奥德蘑属复合群中分开。现知干蘑属包含六种,本文提供了这些种的检索表,并对各种进行了描述、图示或讨论。其中,硬毛干蘑(X.strigosa)是一新种,它与干蘑(X.pudens)和中华干蘑(X.sinopudens)相似,但与干蘑不同在于其担孢子较长,锁状联合稀少,侧生囊状体顶端壁薄;与中华干蘑的区别在于其侧生囊状体壁厚,顶端有头状至近头状膨大并覆盖有结晶,担孢子宽椭圆形至椭圆形。中华干蘑和小刺干蘑(X.setulosa)分别为我国和哥斯达黎加的新记录种。干蘑属虽然广布北半球,但未见东亚-美洲或欧亚广布的物种分布类型。     

狭义干蘑属( 蘑菇目) 概要及新的系统学处理

对狭义干蘑属( Xerula s . str .) 的范围进行了重新界定, 该属仅包括模式种干蘑( X. pudens) 及其周围的几个种。属的特征是担子果金钱菌状, 有假根; 菌盖及菌柄表面干燥, 被有黄色至褐色直立、披针形的刚毛; 菌褶边缘无不育带; 子实层有近梭形的小担子。这些特征可以将干蘑属的物种从干蘑属- 小奥德蘑属复合群中分开。现知干蘑属包含六种, 本文提供了这些种的检索表, 并对各种进行了描述、图示或讨论。其中, 硬毛干蘑( X. strigosa) 是一新种, 它与干蘑( X. pudens) 和中华干蘑( X. sinopudens) 相似, 但与干蘑不同在于其担孢子较长, 锁状联合稀少, 侧生囊状体顶端壁薄; 与中华干蘑的区别在于其侧生囊状体壁厚, 顶端有头状至近头状膨大并覆盖有结晶, 担孢子宽椭圆形至椭圆形。中华干蘑和小刺干蘑( X. setulosa) 分别为我国和哥斯达黎加的新记录种。干蘑属虽然广布北半球, 但未见东亚- 美洲或欧亚广布的物种分布类型。     

MOLECULAR EVIDENCE FROM nrDNA ITS SEQUENCES THAT LAMINARIOCOLAX (PHAEOPHYCEAE, ECTOCARPALES SENSU LATO) IS A WORLDWIDE CLADE OF CLOSELY RELATED KELP ENDOPHYTES

Marine brown algae living as endophytes in macroalgae are morphologically simple and their taxonomy is particularly difficult. A molecular phylogeny for endophytic taxa isolated from kelps and red algae, and for putative epiphytic and free-living relatives, was inferred from partial small subunit and complete internal transcribed spacer nuclear ribosomal DNA sequences. It has revealed the following results. (1) Three species of endophytes isolated from members of the Laminariales are closely related. They form a clade together with the epi-endophytic species Laminariocolax tomentosoides (Farlow) Kylin. Members of the clade possess uniseriate plurilocular sporangia, and they may form erect filaments. Laminariocolax eckloniae sp. nov., occurring in the South African host Ecklonia maxima (Osbeck) Papenfuss, is described. The new combinations, Laminariocolax aecidioides (Rosenvinge) comb. nov. and L. macrocystis (Peters) comb. nov., are proposed for two taxa previously classified in Gononema and Streblonema , respectively. (2) The genus Laminariocolax occurs worldwide in temperate areas, and the phylogeny of the taxa studied is in agreement with biogeographic distribution. (3) Laminariocolax belongs to the Ectocarpales sensu lato. The genus is more closely related to Chordaria than to Dictyosiphon, Ectocarpus, or Scytosiphon . (4) Two brown endophytes ( Streblonema spp.), isolated from red algae, are closely related to each other and may form a sister clade to Laminariocolax .     

中国葡萄座腔菌属分类研究概况

葡萄座腔菌属Botryosphaeria及其无性型包括许多植物病原菌以及有经济价值的类群,是腔子囊菌的重要类群.简要回顾了我国该属真菌的分类研究历史,概述了近年来取得的相关研究进展,提供了中国已知种类检索表,展示建立在分类学基础上的分子系统学研究结果,即葡萄座腔菌属Botryosphaeria是多谱系的类群,可分为多个分支,各分支代表其不同的无性型类群.对开展该类真菌的物种多样性和分子系统学研究进行了展望.     

Cryptadelphia (Trichosphaeriales), a new genus for holomorphs with Brachysporium anamorphs and clarification of the taxonomic status of Wallrothiella

The genus Cryptadelphia (Trichosphaeriales) is described for the presumed teleomorphs of six species of the dematiaceous hyphomycete genus Brachysporium. Cryptadelphia is characterized by the production of dark, leathery perithecia with two-layered walls, cylindrical, long-stipitate asci with eight hyaline, initially aseptate but ultimately 1-septate as-cospores. Zigno?lla groenendalensis is transferred to Cryptadelphia with Wallrothiella melanostigmoides as a synonym. Five new species, i.e., Cryptadelphia abietis, C. brevior, C. obovata, C. pendulispora and C. polyseptata, with their respective Brachysporium anamorphs, are described and illustrated. Phylogenetic analysis of partial large subunit rDNA sequences of two species supports the monophyly of Cryptadelphia and suggests a relationship with the Trichosphaeriales. The monophyly of the Trichosphaeriaceae and the relationships between the Trichosphaeriaceae, Annula-tascaceae and the Sordariales can be questioned, but a conclusive assessment requires phylogenetic analysis of more related taxa. The genus Wallrothiella is redescribed based on new collections of W. congregata and is characterized by the production of tiny, globose ascospores in cylindrical asci. The new collection is proposed as a neotype for the genus.