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1.
Lyudmila A. Romanenko Naoto Tanaka Vassilii I. Svetashev Natalia I. Kalinovskaya 《Archives of microbiology》2013,195(4):247-254
A novel bacterial strain Sl 79T was isolated from a deep surface sediment sample obtained from the Sea of Japan and investigated by phenotypic and molecular methods. The bacterium Sl 79T was Gram-positive, facultatively anaerobic, spore-forming, motile and able to form two different types of colonies. It contained the major menaquinone MK-7 and anteiso-C15:0 followed by iso-C15:0 as predominant fatty acids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Sl 79T belonged to the genus Paenibacillus where it clustered to Paenibacillus apiarius NRRL NRS-1438T with a sequence similarity of 97.7 % and sharing sequence similarities below than 96.7 % to other validly named Paenibacillus species. Strain Sl 79T was found to possess a remarkable inhibitory activity against indicatory microorganisms. On the basis of combined spectral analyses, strain Paenibacillus sp. Sl 79T was established to produce isocoumarin and novel peptide antibiotics. On the basis of DNA–DNA relatedness, phenotypic and phylogenetic data obtained, it was concluded that strain Sl 79T represents a novel species, Paenibacillus profundus sp. nov. with the type strain Sl 79T = KMM 9420T = NRIC 0885T. 相似文献
2.
Yu Zhou Sheng Gao Da-Qiao Wei Ling-Ling Yang Xing Huang Jie He Yun-Jiao Zhang Shu-Kun Tang Wen-Jun Li 《Antonie van Leeuwenhoek》2012,102(4):601-609
A Gram-positive, thermophilic, strictly aerobic bacterium, designated WP-1T, was isolated from a sediment sample from a hot spring in Fujian province of China and subjected to a polyphasic taxonomic study. Cells of strain WP-1T were rods (~0.6–0.8 × 2.5–3.5 μm) and motile by means of peritrichous flagella. Endospores were ellipsoidal in terminal or subterminal positions. Strain WP-1T grew at 37–60 °C (optimum 42–45 °C), 0–3 % NaCl (optimum 1 %, w/v) and pH 3.0–9.0 (optimum pH 6.5–7.0). The predominant menaquinone was MK-7. The major fatty acids were anteiso-C15:0, iso-C16:0, C16:0 and anteiso-C17:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two glycolipids, two unidentified phospholipids and two unknown polar lipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid (meso-DAP). The G + C content of the genomic DNA was 52.5 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain WP-1T is a member of the genus Paenibacillus and exhibited sequence similarity of 99.3 % to Paenibacillus macerans DSM 24T and both strains represented a separate lineage from all other Paenibacillus species. However, the level of DNA–DNA relatedness between strain WP-1T and P. macerans DSM 24T was 34.0 ± 4.7 %. On the basis of phylogenetic, physiological and chemotaxonomic analysis data, strain WP-1T is considered to represent as a novel species of the genus Paenibacillus, for which the name Paenibacillus thermophilus sp. nov., is proposed, with the type strain WP-1T (=DSM 24746T = JCM 17693T = CCTCC AB 2011115T). 相似文献
3.
Yu-Juan Liu Li-Juan Long Xiao-Fang Huang Zhi-Qing You Fa-Zuo Wang Jie Li Chang-Jin Kim Xin-Peng Tian Si Zhang 《Antonie van Leeuwenhoek》2013,104(5):829-836
A strictly aerobic, Gram-stain positive, slightly halophilic strain, designated SCSIO 04524T, was isolated from a deep sea sediment sample collected from the northern South China Sea at a depth of 3415 m. The isolate slightly embedded into the medium after 72 h incubation at 30 °C. Growth was found to occur on media with 0–10 % NaCl but extremely weak growth occurred without supplying NaCl. The predominant menaquinone was determined to be MK-7. The major cellular fatty acid identified was iso-C15:0. The diagnostic polar lipids were determined to be diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content was determined to be 38 mol%. 16S rRNA gene sequences analysis showed that this strain had the highest similarities with Bacillus carboniphilus JCM 9731T (94.7 %) and Bacillus endophyticus 2DTT (94.3 %). Phylogenetic analysis revealed that strain SCSIO 04524T formed a distinct lineage with Bacillus chungangensis CAU 348T and B. carboniphilus JCM 9731T. Physiological characteristics including utilization of sole nitrogen and carbon sources, and chemotaxonomic properties of cellular fatty acids and polar lipids could readily distinguish strain SCSIO 04524T from its most closely related species. Based on this polyphasic taxonomic data, a new species, Bacillus oceani sp. nov., is proposed, with the type strain SCSIO 04524T (=DSM 26213T = KCTC 33077T). 相似文献
4.
Jeong Myeong Kim Se Hee Lee Seung Hyeon Lee Eun Jin Choi Che Ok Jeon 《Antonie van Leeuwenhoek》2013,103(1):3-9
A Gram-staining positive, facultative aerobic bacterium, designated strain RH-N24T, was isolated from naked barley in South Korea. Cells of the isolate were observed to be motile rods by means of peritrichous flagella and showed catalase-positive and oxidase-negative reactions. Growth of strain RH-N24T was observed at 4–40 °C (optimum: 35–37 °C) and at pH 5.0–9.0 (optimum: pH 6.0–7.0). Chemotaxonomic data (major isoprenoid quinone: MK-7; DNA G + C content: 53.5 mol %; cell wall type: A1γ-meso-diaminopimelic acid; major fatty acids: anteiso-CB15:0 and CB16:0B) supported the affiliation of the isolate to the genus Paenibacillus. The major cellular polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences also supported the conclusion that strain RH-N24T belonged to the genus Paenibacillus. Comparative 16S rRNA gene sequence analysis showed that strain RH-N24T was most closely related to Paenibacillus hunanensis FeL05T and Paenibacillus illinoisensis NRRL NRS-1356T with similarities of 94.64 and 94.54 %, respectively. On the basis of phenotypic and molecular properties, strain RH-N24T represents a novel species within the genus Paenibacillus for which the name Paenibacillus hordei sp. nov. is proposed. The type strain is RH-N24T (=KACC 15511T = JCM 17570T). 相似文献
5.
Zhi-Qing You Jie Li Sheng Qin Xin-Peng Tian Fa-Zuo Wang Si Zhang Wen-Jun Li 《Antonie van Leeuwenhoek》2013,103(5):963-969
A Gram-positive bacterium, designated SCSIO 15042T, was isolated from a sediment of the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew at 20–60 °C, pH 6.0–10.0 and it could grow with up to 10 % (w/v) NaCl. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and an unknown polar lipid. The only menaquinone was determined to be MK-7. The major fatty acids were identified as C16:1 ω7c/C16:1 ω6c, C16:0, iso-C15:0, anteiso-C15:0, and iso-C16:0. The DNA G+C content of strain SCSIO 15042T was determined to be 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SCSIO 15042T to the genus Bacillus. Levels of 16S rRNA gene sequence similarities between strain SCSIO 15042T and Bacillus herbersteinensis D-1-5aT, Bacillus infantis SMC 4352-1T, Bacillus novalis LMG 21837T and Bacillus drentensis LMG 21831T were 96.2, 96.2, 96.1 and 96.1 %, respectively. Based on the evidence of the present polyphasic study, strain SCSIO 15042T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus abyssalis sp. nov. is proposed. The type strain is SCSIO 15042T (=DSM 25875T = CCTCC AB 2012074T = NBRC 109102T). 相似文献
6.
Selenate reduction rates and kinetics across depth in littoral sediment of the Salton Sea,California
To predict selenium cycling in sediments, it is crucial to identify and quantify the processes leading to selenium sequestration in sediments. More specifically, it is essential to obtain environmentally-relevant kinetic parameters for selenium reduction and information on how they spatially vary in sediments. The Salton Sea (California, USA) is an ideal model system to examine selenium processes in sediments due to its semi-enclosed conditions and increasing selenium concentration over the last century. Selenium enters the Salton Sea mainly as selenate and might be sequestered in the sediment through microbial reduction. To determine the potential selenium sequestration of Salton Sea littoral sediments and which sediment properties are controlling selenate reduction kinetics, we determined the centimeter-scale vertical distribution of potential selenate reduction rates and apparent kinetic parameters (maximum selenate reduction rates, Vmax, and selenate half-saturation concentration, Km) using flow-through reactor (FTR) experiments. We compared sediments from two littoral sites (South and North) and four depth intervals (0–2, 2–4, 4–6 and 6–8 cm). Furthermore, we characterized the selenium fractions in the sediment recovered from the FTR experiments to identify the processes leading to the sequestration of selenium. Our results reveal higher potential for selenium reduction and sequestration in the topmost sediment (0–2 cm) suggesting that microorganisms inhabiting surface sediment are well adapted to reduce selenate entering the Salton Sea. As apparent Km values (103–2144 µM) exceed the average selenium concentration in the overlying water (6–25 nM), in situ selenate reduction is limited by the low availability of selenate and the resident selenate-reducing microorganisms operate well below their Vmax (11 and 43 nmol cm?3 h?1). Selenium speciation after FTR experiments confirms the primary sequestration of reduced biomass-associated and elemental selenium (68–99% of total selenium) in the sediment. Further, the absence of correlation between the tested sediment physical (porosity, bulk density, clay content), chemical (Corg, Ntot, total selenium content) and biological characteristics (abundance of culturable selenate-reducers) with the kinetic parameters of selenate reduction indicates that these sediment characteristics cannot be used as predictors of apparent Vmax or Km. Conclusively, microbial selenate reduction is an important, if not the primary process, leading to the sequestration of reduced selenium in the Salton Sea sediments and making the surficial Salton Sea sediments an important selenium sink. 相似文献
7.
Yi-Guang Chen Song-Ping Hu Shu-Kun Tang Jian-Wu He Jian-Qing Xiao Hong-Yi Zhu Wen-Jun Li 《Antonie van Leeuwenhoek》2011,99(3):473-480
A novel Gram-stain-positive, motile, catalase- and oxidase-positive, endospore-forming, aerobic, rod-shaped bacterium, designated strain JSM 099021T, was isolated from an oyster collected from Naozhou Island in the South China Sea. Growth occurred with 0?C15% (w/v) NaCl (optimum 2?C4%) and at pH 6.0?C10.0 (optimum pH 7.5) and at 10?C45°C (optimum 30?C35°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0. The genomic DNA G + C content was 39.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 099021T belongs to the genus Bacillus, and was most closely related to the type strains of Bacillus halmapalus (sequence similarity 99.0%), Bacillus horikoshii (98.4%) and Bacillus cohnii (98.0%). The combination of phylogenetic analysis, DNA?CDNA hybridization, phenotypic characteristics and chemotaxonomic data supported the proposal that strain JSM 099021T represents a new species of the genus Bacillus, for which the name Bacillus zhanjiangensis sp. nov. is proposed. The type strain was JSM 099021T (=DSM 23010T = KCTC 13713T). 相似文献
8.
A novel nitrogen-fixing bacterium, BJ-18T, was isolated from wheat rhizosphere soil. Strain BJ-18T was observed to be Gram-positive, facultatively anaerobic, motile and rod-shaped (0.4–0.9 μm × 2.0–2.9 μm). Phylogenetic analysis based on a partial nifH gene sequence and an assay for nitrogenase activity showed its nitrogen-fixing capacity. Phylogenetic analysis based on full 16S rRNA gene sequences suggested that strain BJ-18T is a member of the genus Paenibacillus. High similarity of 16S rRNA gene sequence was found between BJ-18T and Paenibacillus peoriae DSM 8320T (99.05 %), Paenibacillus jamilae DSM 13815T (98.86 %), Paenibacillus brasiliensis DSM 13188T (98.55 %), Paenibacillus polymyxa DSM 36T (98.74 %), Paenibacillus terrae DSM 15891T (97.99 %) and Paenibacillus kribbensis JCM 11465T (97.92 %), whereas the similarity was below 96.0 % between BJ-18T and the other Paenibacillus species. DNA–DNA relatedness between strain BJ-18T and P. peoriae DSM 8320T, P. jamilae DSM 13815T, P. brasiliensis DSM 13188T, P. polymyxa DSM 36T, P. kribbensis JCM 11465T and P. terrae DSM 15891T was determined to be 43.6 ± 2.7, 34.2 ± 5.3, 47.9 ± 6.6, 36.8 ± 3.5, 27.4 ± 4.3 and 23.6 ± 4.1 % respectively. The DNA G+C content of BJ-18T was determined to be 45.8 mol %. The major fatty acid was identified as anteiso-C15:0 (67.1 %). The polar lipids present in strain BJ-18T were identified as diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The phenotypic and genotypic characteristics, and DNA–DNA relatedness data, suggest that BJ-18T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus beijingensis sp. nov. (Type strain BJ-18T=DSM25425T=CGMCC 1.12045T) is proposed. 相似文献
9.
Jun Zhang Xiao-Tong Ma Jun-Sheng Gao Cai-Wen Zhang Juan-Juan Zhao Rui-Jie Zhang Li-An Ma Xiao-Xia Zhang 《Antonie van Leeuwenhoek》2017,110(1):69-75
A novel bacterium, strain 1ZS3-15T, was isolated from rhizosphere of rice. Its taxonomic position was investigated using a polyphasic approach. The novel strain was observed to be Gram-stain positive, spore-forming, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1ZS3-15T was recovered within the genus Paenibacillus. It is closely related to Paenibacillus pectinilyticus KCTC 13222T (97.9 % similarity), Paenibacillus frigoriresistens CCTCC AB 2011150T (96.8 %), Paenibacillus alginolyticus JCM 9068T (96.4 %) and Paenibacillus chondroitinus DSM 5051T (95.5 %). The fatty acid profile of strain 1ZS3-15T, which showed a predominance of anteiso-C15:0 and iso-C16:0, supported the allocation of the strain into the genus Paenibacillus. The predominant menaquinone was found to be MK-7. The polar lipids profile of strain 1ZS3-15T was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and two unidentified aminophospholipids. The cell wall peptidoglycan contains meso-diaminopimelic acid. Based on draft genome sequences, the DNA–DNA relatedness between strain 1ZS3-15T and the closely related species P. pectinilyticus KCTC 13222T are 24.2 ± 1.0 %, and the Average Nucleotide Identity values between the strains are 78.9 ± 0.1 %, which demonstrated that this isolate represents a new species in the genus Paenibacillus. The DNA G+C content was determined to be 45.3 mol%, which is within the range reported for Paenibacillus species. Characterisation by genotypic, chemotaxonomic and phenotypic analysis indicated that strain 1ZS3-15T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus oryzisoli sp. nov. is proposed. The type strain is 1ZS3-15T (= ACCC 19783T = JCM 30487T). 相似文献
10.
Bioavailability and metabolism of fucoxanthin in rats: structural characterization of metabolites by LC-MS (APCI) 总被引:1,自引:0,他引:1
Ravi Kumar Sangeetha Narayan Bhaskar Sounder Divakar Vallikannan Baskaran 《Molecular and cellular biochemistry》2010,333(1-2):299-310
This study reports bioavailability and metabolism of fucoxanthin (FUCO) from brown algae Padina tetrastromatica in rats. Rats were divided into two groups (n = 25/group). Group one was fed basal diet (control) while the group two received retinol deficient diet (RD group) for 8 weeks. After confirmed RD in blood (0.53 μmol/l), rats were further sub-grouped (n = 5/sub group), intubated a dose of FUCO (0.83 μmol) and killed after 0, 2, 4, 6 and 8 h. The plasma levels (area under curve/8 h) of FUCO (fucoxanthinol (FUOH) + amarouciaxanthin (AAx)) was 2.93 (RD group) and 2.74 pmol/dl (control), respectively. No newly formed retinol was detected in RD rats intubated with FUCO. Besides FUOH (m/z 617 (M+H)+) and AAx (m/z 617 (M+H?)+), other deacetylated, hydrolyzed and demethylated metabolites of bearing molecular mass at m/z 600.6 (FUOH–H2O), m/z 597 (AAx–H2O), m/z 579 (AAx–2H2O+1), m/z 551 (AAx–2H2O–2CH3+2) and m/z 523 (AAx–2H2O–4CH3+4) were also detected in plasma and liver by LC-MS (APCI). Although biological functions of FUCO metabolites need thorough investigation, this is the first detailed report on FUCO metabolites in rats. 相似文献
11.
Shashi Kiran Mohit Kumar Swarnkar Shanmugam Mayilraj Rupinder Tewari Arvind Gulati 《Systematic and applied microbiology》2017,40(7):430-439
The assessment of bacterial diversity and bioprospection of the high-altitude lake Suraj Tal microorganisms for potent antimicrobial activities revealed the presence of two Gram-stain-variable, endospore-forming, rod-shaped, aerobic bacteria, namely IHBB 9852T and IHBB 9951. Phylogenetic analysis based on 16S rRNA gene sequence showed the affiliation of strains IHBB 9852T and IHBB 9951 within the genus Paenibacillus, exhibiting the highest sequence similarity to Paenibacillus lactis DSM 15596T (97.8% and 97.7%) and less than 95.9% similarity to other species of the genus Paenibacillus. DNA-DNA relatedness among strains IHBB 9852T and IHBB 9951 was 90.2%, and with P. lactis DSM 15596T, was 52.7% and 52.4%, respectively. The novel strains contain anteiso-C15:0, iso-C15:0, C16:0 and iso-C16:0 as major fatty acids, and phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol were predominant polar lipids. The DNA G+C content for IHBB 9852T and IHBB 9951 was 52.1 and 52.2 mol%. Based on the results of phenotypic and genomic characterisations, we concluded that strains IHBB 9852T and IHBB 9951 belong to a novel Paenibacillus species, for which the name Paenibacillus ihbetae sp. nov. is proposed. The type strain is IHBB 9852T (=MTCC 12459T = MCC 2795T = JCM 31131T = KACC 19072T; DPD TaxonNumber TA00046) and IHBB 9951 (=MTCC 12458 = MCC 2794 = JCM 31132 = KACC 19073) is a reference strain. 相似文献
12.
An actinomycete strain Nocardiopsis sp. DN-K15 showing high inulinolytic activity was isolated from marine sediment of Jiaozhou Bay in China. Under optimal conditions, Nocardiopsis sp. DN-K15 produced 25.1 U/ml of inulinase within 60 h of fermentation at shake flask level, which was 2.7-fold higher than the level in the basal medium. The optimal pH and temperature of the inulinase from strain DN-K15 were determined to be 60 °C and pH 8.0, respectively. The inulinase was highly active over a wide pH range (5.0–11.0) and retained more than 81 % of residual activity after incubation at 60 °C for 1 h, indicating its alkali-tolerant and thermostable nature. Thin layer chromatography analysis revealed that fructose was the main product of inulin hydrolysis, indicating its exoinulinase activity. The high yield of extracellular inulinase combined with its novel enzymatic property made Nocardiopsis sp. DN-K15 a potential candidate in biotechnological and industrial applications. 相似文献
13.
Miao Gao Lin-qi Xie Ya-xiong Wang Jian Chen Jing Xu Xiao-xia Zhang Xin-hua Sui Jun-lian Gao Jian-guang Sun 《Antonie van Leeuwenhoek》2012,102(4):689-694
A novel Gram-positive, rod-shaped, motile, spore-forming, nitrogen-fixing bacterium, designated strain 7188T, was isolated from jujube rhizosphere soil in Beijing, China. The strain grew at 4–40 °C and pH 6–12, with an optimum of 30 °C and pH 7.0, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 7188T is a member of the genus Paenibacillus. Levels of 16S rRNA gene sequence similarities between strain 7188T and the type strains of all recognized members of the genus Paenibacillus were below 96 %. The major cellular fatty acids were anteiso-C15:0, anteiso-C17:0 and C16:0. The predominant menaquinone was MK-7. The DNA G+C content of strain 7188T was 60.3 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminophospholipids. The diamino acid in the cell wall peptidoglycan is meso-diaminopimelic acid. On the basis of these results, strain 7188T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus beijingensis sp. nov. is proposed. The type strain is 7188T (=ACCC 03082T = DSM 24997T). 相似文献
14.
Xiao-Fang Huang Fa-Zuo Wang Wei Zhang Jie Li Juan Ling Jian Yang Jun-De Dong Xin-Peng Tian 《Antonie van Leeuwenhoek》2014,106(6):1089-1095
A Gram-positive, rod-shaped bacterium, designated strain SCSIO N0306T, was isolated from an abyssal sediment sample collected from the Indian Ocean. The isolate was found to grow optimally at 0–2 % (w/v) NaCl, pH 7.0 and 30 °C. Comparative analysis of the 16S rRNA gene sequence showed that the isolate SCSIO N0306T belongs phylogenetically to the genus Paenibacillus, and to be most closely related to P. algorifonticola XJ259T (with 95.47 % sequence similarity), sharing less than 95.0 % sequence similarity with all other taxa of this genus. Chemotaxonomic analysis revealed MK-7 as the major isoprenoid quinone, the DNA G+C content was determined to be 45.5 mol%, and anteiso-C15:0, C16:0, and iso-C15:0 were identified as the major fatty acids. On the basis of this polyphasic taxonomic data, isolate SCSIO N0306T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus abyssi sp. nov. is proposed. The type strain is SCSIO N0306T (= DSM 26238T = CGMCC 1.12987T). 相似文献
15.
Microorganisms may have a direct action on the fate of organic/inorganic ions in the environment through biosorption, detoxification or bioaccumulation processes. In the present study, the marine actinobacterial Nocardiopsis sp. 13H strain, isolated from different nuclear power plant (NPP) sites, India, tested for long half-life radionuclide of strontium (Sr+) was studied. Nocardiopsis sp. 13H could remove 93.6 ± 0.8% of Sr+ from test solutions containing 10 mM SrCl2. The biosorption of Sr+ was studied for optimum condition of pH, temperature and time interval of remediation. Further, Sr+ adsorption was confirmed by scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS). In addition, with the presence of Sr+, extracellular polymeric substances (EPSs) were also found more and suggested interaction of EPS surface active assemblages being involved in radionuclide remediation. Fourier transform infrared (FTIR) study confirmed that Nocardiopsis sp. 13H cell surfaces are involved in Sr+ adsorption with the presence of dominant functional groups such as carboxyl, hydroxyl and amide groups. The results conclude that marine actinobacterial Nocardiopsis sp. 13H could be useful for the treatment of long half-life radioisotope pollution as an eco-friendly biosorbent in marine environmental cleanup. 相似文献
16.
An actinomycete strain 7326 producing cold-adapted α-amylase was isolated from the deep sea sediment of Prydz Bay, Antarctic.
It was identified as Nocardiopsis based on morphology, 16S rRNA gene sequence analysis, and physiological and biochemical characteristics. Sodium dodecyl sulfate-polyacrylamide
gel electrophoresis and zymogram activity staining of purified amylase showed a single band equal to a molecular mass of about
55 kDa. The optimal activity temperature of Nocardiopsis sp. 7326 amylase was 35°C, and the activity decreased dramatically at temperatures above 45°C. The enzyme was stable between
pH 5 and 10, and exhibited a maximal activity at pH 8.0. Ca2+, Mn2+, Mg2+, Cu2+, and Co2+ stimulated the activity of the enzyme significantly, and Rb2+, Hg2+, and EDTA inhibited the activity. The hydrolysates of soluble starch by the enzyme were mainly glucose, maltose, and maltotriose.
This is the first report on the isolation and characterization of cold-adapted amylase from Nocardiopsis sp. 相似文献
17.
Jian-Bo Xie Li-Hong Zhang Yu-Guang Zhou Hong-Can Liu San-Feng Chen 《Antonie van Leeuwenhoek》2012,102(4):735-741
A nitrogen-fixing bacterium, designated strain gs65T, was isolated from a rhizosphere soil sample of Caragana kansuensis Pojark. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain gs65T is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarity were found between strain gs65T and Paenibacillus borealis DSM 13188T (97.5 %), Paenibacillus odorifer ATCC BAA-93T (97.3 %), Paenibacillus durus DSM 1735T (97.0 %) and Paenibacillus sophorae DSM23020T (96.9 %). Levels of 16S rRNA gene sequence similarity between strain gs65T and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA–DNA relatedness between strain gs65T and P. borealis DSM 13188T, P. odorifer ATCC BAA-93T (97.3 %), P. durus DSM 1735T and P. sophorae DSM23020T were 35.9, 38.0, 34.2 and 35.5 % respectively. The DNA G+C content of strain gs65T was determined to be 51.6 mol%. The major fatty acids were found to be iso-C14:0, anteiso-C15:0 and iso-C16:0. On the basis of its phenotypic characteristics and levels of DNA–DNA hybridization, strain gs65T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus taohuashanense sp. nov. is proposed. The type strain is gs65T (=CGMCC 1.12175T = DSM 25809T). 相似文献
18.
S. Doni C. Macci E. Peruzzi R. Iannelli B. Ceccanti G. Masciandaro 《Biodegradation》2013,24(4):499-512
The continuous stream of sediments, dredged from harbors and waterways for keeping shipping traffic efficiency, is a considerable ongoing problem recognized worldwide. This problem gets worse as most of the sediments dredged from commercial ports and waterways turn out to be polluted by a wide range of organic and inorganic contaminants. In this study, phytoremediation was explored as a sustainable reclamation technology for turning slightly-polluted brackish dredged sediments into a matrix feasible for productive use. To test this possibility, a phytoremediation experimentation was carried out in containers of about 0.7 m3 each, filled with brackish dredged sediments contaminated by heavy metals and hydrocarbons. The sediments were pre-conditioned by adding an agronomic soil (30 % v/v) to improve their clayey granulometric composition, and by topping the mixture with high quality compost (4 kg m?2) to favour the initial adaptation of the selected vegetal species. The following plant treatments were tested: (1) Paspalum vaginatum, (2) Phragmites australis, (3) Spartium junceum + P. vaginatum, (4) Nerium oleander + P. vaginatum, (5) Tamarix gallica + P. vaginatum, and (6) unplanted control. Eighteen months after the beginning of the experimentation, all the plant species were found in healthy condition and well developed. Throughout the whole experiment, the monitored biological parameters (total microbial population and dehydrogenase activity) were generally observed as constantly increasing in all the planted sediments more than in the control, pointing out an improvement of the chemico-physical conditions of both microorganisms and plants. The concentration decrease of organic and inorganic contaminants (>35 and 20 %, respectively) in the treatments with plants, particularly in the T. gallica + P. vaginatum, confirmed the importance of the root-microorganism interaction in activating the decontamination processes. Finally, the healthy state of the plants and the sediment characteristics, approaching those of an uncontaminated natural soil (technosoil), indicated the efficiency and success of this technology for brackish sediments reclamation. 相似文献
19.
Juliana L. França Marcelo R. Pinto Malson N. Lucena Daniela P. Garçon Wagner C. Valenti John C. McNamara Francisco A. Leone 《The Journal of membrane biology》2013,246(7):529-543
The stimulation by Mg2+, Na+, K+, NH4 +, and ATP of (Na+, K+)-ATPase activity in a gill microsomal fraction from the freshwater prawn Macrobrachium rosenbergii was examined. Immunofluorescence labeling revealed that the (Na+, K+)-ATPase α-subunit is distributed predominantly within the intralamellar septum, while Western blotting revealed a single α-subunit isoform of about 108 kDa M r. Under saturating Mg2+, Na+, and K+ concentrations, the enzyme hydrolyzed ATP, obeying cooperative kinetics with V M = 115.0 ± 2.3 U mg?1, K 0.5 = 0.10 ± 0.01 mmol L?1. Stimulation by Na+ (V M = 110.0 ± 3.3 U mg?1, K 0.5 = 1.30 ± 0.03 mmol L?1), Mg2+ (V M = 115.0 ± 4.6 U mg?1, K 0.5 = 0.96 ± 0.03 mmol L?1), NH4 + (V M = 141.0 ± 5.6 U mg?1, K 0.5 = 1.90 ± 0.04 mmol L?1), and K+ (V M = 120.0 ± 2.4 U mg?1, K M = 2.74 ± 0.08 mmol L?1) followed single saturation curves and, except for K+, exhibited site–site interaction kinetics. Ouabain inhibited ATPase activity by around 73 % with K I = 12.4 ± 1.3 mol L?1. Complementary inhibition studies suggest the presence of F0F1–, Na+-, or K+-ATPases, but not V(H+)- or Ca2+-ATPases, in the gill microsomal preparation. K+ and NH4 + synergistically stimulated enzyme activity (≈25 %), suggesting that these ions bind to different sites on the molecule. We propose a mechanism for the stimulation by both NH4 +, and K+ of the gill enzyme. 相似文献
20.
Kurt Haselwandter Gerlinde Häninger Markus Ganzera Hubertus Haas Graeme Nicholson Günther Winkelmann 《Biometals》2013,26(6):969-979
A screening for siderophores produced by the ectomycorrhizal fungi Laccaria laccata and Laccaria bicolor in synthetic low iron medium revealed the release of several different hydroxamate siderophores of which four major siderophores could be identified by high resolution mass spectrometry. While ferricrocin, coprogen and triacetylfusarinine C were assigned as well as other known fungal siderophores, a major peak of the siderophore mixture revealed an average molecular mass of 797 for the iron-loaded compound. High resolution mass spectrometry indicated an absolute mass of m/z = 798.30973 ([M + H]+). With a relative error of Δ = 0.56 ppm this corresponds to linear fusigen (C33H52N6O13Fe; MW = 797.3). The production of large amounts of linear fusigen by these basidiomycetous mycorrhizal fungi may possibly explain the observed suppression of plant pathogenic Fusarium species. For comparative purposes Fusarium roseum was included in this study as a well known producer of cyclic and linear fusigen. 相似文献