共查询到20条相似文献,搜索用时 15 毫秒
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J Spychala A G Zimmermann B S Mitchell 《The Journal of biological chemistry》1999,274(32):22705-22712
We have isolated the 5' region of the ecto-5'-nucleotidase (low K(m) 5'-NT) gene and established that a 969-base pair (bp) fragment confers cell-specific expression of a CAT reporter gene that correlates with the expression of endogenous ecto-5'-NT mRNA and enzymatic activity. A 768-bp upstream negative regulatory region has been identified that conferred lymphocyte-specific negative regulation in a heterologous system with a 244-bp deoxycytidine kinase core promoter. DNase I footprinting identified several protected areas including Sp1, Sp1/AP-2, and cAMP response element (CRE) binding sites within the 201-bp core promoter region and Sp1, NRE-2a, TCF-1/LEF-1, and Sp1/NF-AT binding sites in the upstream regulatory region. Whereas the CRE site was essential in mediating the negative activity of the upstream regulatory region in Jurkat but not in HeLa cells, mutation of the Sp1/AP-2 site decreased promoter activity in both cell lines. Electrophoretic mobility shift assay analysis of proteins binding to the CRE site identified both ATF-1 and ATF-2 in Jurkat cells. Finally, phorbol 12-myristate 13-acetate increased the activity of both the core and the 969-bp promoter fragments, and this increase was abrogated by mutations at the CRE site. In summary, we have identified a tissue-specific regulatory region 5' of the ecto-5'-NT core promoter that requires the presence of a functional CRE site within the basal promoter for its suppressive activity. 相似文献
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Sp1 transactivation of the TCL1 oncogene 总被引:3,自引:0,他引:3
French SW Malone CS Shen RR Renard M Henson SE Miner MD Wall R Teitell MA 《The Journal of biological chemistry》2003,278(2):948-955
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Identification and regulation of human PDE5A gene promoter 总被引:7,自引:0,他引:7
Lin CS Chow S Lau A Tu R Lue TF 《Biochemical and biophysical research communications》2001,280(3):684-692
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U Malipiero M H?ller U Werner A Fontana 《Biochemical and biophysical research communications》1990,171(3):1145-1151
Human glioblastoma cells secrete a factor termed glioblastoma derived T cell suppressor factor (G-TsF) or transforming growth factor beta 2 (TGF-beta 2) which inhibits the response of T cells to mitogenic or antigenic stimulation. In the present study we isolated the promoter region of the G-TsF/TGF-beta 2 gene. The promoter region shares no homology to the promoter of the TGF-beta 1 or the 5' region of the TGF-beta 3 gene and harbours several familiar DNA motifs, including the cytokine-1 region, an octamer-like sequence, Sp1- and AP-2-like elements and a putative NF-kappa B site. In contrast to the TGF-beta 1 gene, the G-TsF/TGF-beta 2 gene contains three TATA-like sequences but lacks an AP-1 site. To understand the cell type specificity of expression of G-TsF/TGF-beta 2, the individual contribution of the DNA elements detected in the promoter has to be analysed in further studies. 相似文献
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Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3. 总被引:5,自引:0,他引:5
Mingfeng Liu Julian L Leibowitz David A Clark Michael Mendicino Qin Ning Jin Wen Ding Cheryl D'Abreo Laisum Fung Philip A Marsden Gary A Levy 《European journal of biochemistry》2003,270(10):2274-2286
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