以米糠蛋白为乳化剂的β-胡萝卜素纳米乳液超声乳化制备工艺优化

为提高米糠蛋白的利用价值及应用意义,本研究拟采用超声乳化法制备以米糠蛋白为乳化剂的β-胡萝卜素纳米乳液,本研究采用响应面优化法获取了米糠蛋白纳米乳液超声乳化制备工艺:超声温度50℃,超声功率490 W,超声时间280 s和米糠蛋白浓度4.5%,在此条件下米糠蛋白纳米乳液的平均粒径为190 nm,乳化产率为88.1%,兼具较强的稳定性。研究结果将为新型功能性食品、纳米食品的开发提供技术途径及研发思路。     

工业化米糠蛋白制取的工艺研究

通过考察欧、美几个稻谷加工厂,以及日本、韩国等发达国家米糠蛋白制取的先进工艺,整理出工业化米糠蛋白制取的工艺：脱脂米糠经过两次浸出和分离后,米糠蛋白溶解于液相中,液相进行酸沉分离提取出米糠蛋白,再对米糠蛋白进行杀菌和干燥,从而获得了优质的食用米糠蛋白。     

植物蛋白新资源的开发

随着我国人口的日益增长,特别是近年来人们对高蛋白食品需求的急剧增加,愈来愈迫使人们去寻找和开发新的蛋白资源。据估计,在已开发的蛋白资源中,植物蛋白资源约占80％,而新的植物蛋白资源还在不断的开发,当前最引人重视的是米糠蛋白资源、叶蛋白资源的开发和有毒饼粕的去毒。     

生物酶法提取米糠蛋白的研究进展

米糠蛋白具有低过敏性、营养价值高等突出优势,是一种新型的优质植物蛋白。米糠蛋白一般以米糠为原料,主要提取方法有碱法提取、物理法提取以及生物酶法提取等,其中,生物酶法由于反应条件温和、蛋白产品品质高等优势备受关注。针对此,本文对生物酶法提取米糠蛋白方法进行了综述,包括酶制剂种类以及酶用量、温度、pH值和料液比等因素对酶解反应的影响规律等,并对生物酶法提取米糠蛋白的前景进行了展望。     

从作物废弃部分提取浓缩叶蛋白

许多植物是为了生产果实、块茎或根等目的而栽培的,有许多部分被废弃而没有利用。许多欧洲国家已成功地开发出利用作废弃物提取浓缩叶蛋白的技术,浓缩叶蛋白已作为商品出售。大豆、马铃薯、豌豆、蕃茄、黄瓜等许多植物的绿色废弃部分都可作为生产浓缩叶蛋白的原料。机械化提取过程包括成浆、压汁、加热使蛋白质凝固、过滤、将凝聚物干燥等步骤,得到高质量的蛋白质。每公顷马铃     

从米糠中提取降血管紧张素转换酶抑制剂

采用盐析法提取米糠蛋白,分别选用胃蛋白酶、胰蛋白酶、木瓜蛋白酶等单独或联合水解米糠蛋白,以从酶解米糠蛋白中分离获得具有血管紧张素转换酶（ACE）抑制活性的短肽组分。经Sephadex G-15凝胶层析和SP—Sephadex C-25离子交换层析分离各酶解组分,并检测各组分的ACE抑制活性。结果表明,米糠蛋白的酶解分离产物中含有较强ACE抑制活性的组分,其中经胃蛋白酶和胰蛋白酶共同水解时得到的小分子量寡肽组分的抑制活性最强,为后续对其进行结构分析奠定了基础。     

米糠蛋白抗氧化活性肽的制备

以水解度(DH％)和对DPPH自由基清除率为指标,筛选出制备米糠蛋白抗氧化活性肽的最适蛋白酶.研究最适蛋白酶的酶解条件,探讨底物浓度、蛋白酶的加入量、pH值、酶解时间等因素对水解度(DH％)和DPPH自由基清除率的影响;在单因素基础上采用Box-Behnken响应曲面中心组合设计法,对酶解米糠蛋白的工艺进行优化.试验结果表明,在加酶量13970.82 U/g,时间3.05h,底物浓度4.97％的水解条件下,米糠蛋白的水解度能够达到23.67％,活性肽对DPPH自由基清除率达到64.26％.     

稻米及其副产品高效增值深加工技术

项目简介：1、以米糠为原料,研究米糠健康食品的无“三废”排放和不添加任何化学添加剂的清洁生产技术,其中米糠稳定化技术的先进性超过美国利普曼公司、Rice—X公司,产品口感、营养价值和成本优于同类知名产品（高乐高、阿华田、美乐等）。授权发明专利2项。2、以低值早籼米及其副产品为原料,采用生物和物理锻炼技术,研究低过敏性蛋白和抗性淀粉的生产技术,     

从我国实际出发,大力开发单细胞蛋白资源

开发单细胞蛋白资源,发展单细胞蛋白工业,这是开辟人类食物新来源的重要途径之一,是件具有战略意义的大事。单细胞蛋白不仅蛋白质含量丰富,营养效果良好,可以用来解决蛋白质食物和饲料的匮乏,而且因它能够以多种废弃资源为原料,进行物质再循环,所以也是加强环境保护、变废为宝的好途径。     

微波处理对米糠蛋白结构及功能性的影响北大核心CSCD

本研究对微波处理下米糠蛋白的结构及功能性变化进行研究,结果表明:随着微波强度增大,α-螺旋和β-转角结构含量先降低后增加,无规卷曲结构含量呈相反变化,而β-折叠结构含量总体降低;反式-扭曲-反式和扭曲-扭曲-扭曲模式含量先增大后降低,扭曲-扭曲-反式振动模式含量先减少后增加。微波处理对酪氨酸的分布状态影响较弱,而色氨酸低微波功率处理下呈"暴露态",高微波功率处理下呈"包埋态"。微波处理后米糠蛋白的溶解性、乳化性、起泡性均有所增大,但在高功率呈降低趋势。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.