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1.
Plant cell and tissue cultures can be used for the synthesis and production of secondary metabolites like colors, flavors, and sweeteners. Most often, plant cell cultures fail to produce the desired products. In such cases strategies to improve the production of secondary metabolites must be considered.
Plant cell culture technology has now reached the point where a variety of culture types can be critically assessed as potential sources of existing and novel flavors and pigments. This brief review gives examples where progress has been made in the development of plant tissue culture systems. 相似文献
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石竹科植物组织培养与细胞工程 总被引:4,自引:0,他引:4
近年来,植物组织培养与细胞工程研究在石竹科植物上取得了一定进展。现从组织培养、原生质体培养和体细胞杂交、单倍体育种、试管开花、转基因等5个方面对其进行综述,并展望了石竹科植物在组织培养和细胞工程研究方面的发展前景。 相似文献
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Effect of medium acidity on growth and rooting of different plant species growing in vitro 总被引:2,自引:0,他引:2
C. Leifert Samantha Pryce P. J. Lumsden W. M. Waites 《Plant Cell, Tissue and Organ Culture》1992,30(3):171-179
Micropropagated Choisya, Daphne, Delphinium, Hemerocallis, Hosta, Iris and Photinia were found to adjust the pH of Murashige and Skoog's plant tissue culture medium (initial pH 5.6 or 3.5) to different values depending on the species. When plant growth and rooting rates were determined after plants had been grown on media initially adjusted or buffered to values between 2.6 and 5.7 the different plant species were also found to have distinct pH requirements for optimal growth and/or rooting rates.Abbreviations MS
Murashige & Skoog's (1962) medium
- MS19
MS with additionally 10 g l–1 sucrose
- 80 mg l–1
adenine sulphate and 130.9 mg l–1 NaH2PO4
- BA
6-benzyladenine
- NAA
1-naphthyl-acetic acid
- IBA
3-indole-butyric acid
- IAA
3-indole-acetic acid
- 2iP
N6(2-isopentyl) adenine 相似文献
5.
Giovanni Iapichino Tony H. H. Chen Leslie H. Fuchigami 《Plant Cell, Tissue and Organ Culture》1991,27(1):37-43
The influence of the source of plant material (greenhouse-grown plants or in vitro shoot cultures), the type of tissue explant (shoot-tip, single-node stem segment, whole leaf, leaf strip or half-leaf section) and growth regulator concentration on shoot regeneration from somatic tissue of Rhododendron laetum × aurigeranum was evaluated. No regeneration response was obtained on explants from greenhouse-grown plants. Adventitious shoots were obtained from callus produced at the basal end of shoot-tip and single-node stem segment explants derived from in vitro-grown shoots cultured on Anderson's medium supplemented with 22.8 M IAA and 73.8 M 2iP. The greatest percentage of adventitious shoot regeneration (77%) was induced on leaf sections cultured in the presence of 22.8 M IAA and 147.6 M 2iP. Plant regeneration was accomplished with minimal callus formation. This technique represents a further step toward gene manipulation of Rhododendron.Abbreviations IAA
1-H-Indole-3-acetic acid
- 2iP
N-(3-methyl-2-Butenyl)-1H-purin-6 amine 相似文献
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Summary A method has been developed for the induction of adventitious shoots from leaf tissue of Echinacea pallida with subsequent whole-plant regeneration. Proliferating callus and shoot cultures were derived from leaf tissue explants
placed on Murashige and Skoog medium supplemented with 6-benzylaminopurine and naphthaleneacetic acid combinations. The optimum
shoot regeneration frequency (63%) and number of shoots per explant (2.3 shoots per explant) was achieved using media supplemented
with 26.6 μM 6-benzylaminopurine and 0.11 μM naphthaleneacetic acid. Rooting of regenerated shoot explants was successful on Murashige and Skoog medium, both with and
without the addition of indole-3-butyric acid. All plantlets survived acclimatization, producing phenotypically normal plants
in the greenhouse. This study demonstrates that leaf tissue of E. pallida is competent for adventitious shoot regeneration and establishes a useful method for the micropropagation of this important
medicinal plant. 相似文献
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通气在动植物细胞或组织生物反应器培养过程中起着至关重要的作用,而同时通气过程所产生的机械损伤力亦可对细胞造成直接的伤害,因此,通气方式是动植物细胞或组织生物反应器培养过程设计与工程放大的关键技术之一。本文综述了动植物细胞或组织生物反应器悬浮培养过程中三种主要通气(异养培养时又称供氧)方式的结构特点,及其对气液传质、生物量、代谢产物量和细胞损伤的影响,以及改进的新型通气方式和几种通气方式的融合并用。 相似文献
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Shatha M. Al-Matar Jameel M. Al-Khayri Mohanjeet S. Brar Gerald L. Klingaman 《In vitro cellular & developmental biology. Plant》1999,35(3):245-248
Summary A system for micropropagation of Epimedium grandiflorum Morr. from rachis explants was developed. Explants were cultured onto Murashige and Skoog (MS) basal salts medium supplemented
with (per L) 100 mg myo-inositol, 2 mg pyridoxine-HCl, 2 mg nicotinic acid, 0.40 mg thiamine-HCl, 30 g sucrose, and 2 g Phytagel. The medium also
contained 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.1, 0.2, or 0.25 mg/L (0.5, 0.9, or 1.1 μM) combined with either N6-benzyladenine (BA) or 2-isopentenyl adenine (2ip) at 2.5, 5, or 10 mg/L (11.1, 22.2, or 44.4 μM BA or 12.3, 24.6, or 49.2 μM 2iP). Cultures were maintained at a 16-h photoperiod (40 μmol/m2/s) and 23±2° C. Callogenesis preceded shoot regeneration. Callus formation increased with higher 2,4-D concentrations. The
highest percent regeneration, 83% of explants, was obtained on 10 mg BA per L (44.4 μM) combined with 0.25 mg 2,4-D per L (1.1 μM). The maximum number of shoots, 15 per explant, was obtained from explants cultured on a medium containing 0.1 mg 2,4-D per
L (0.45 μM) combined with 2.5 mg BA per L (11.1 μM). Maximum shoot length, 0.4 cm, was obtained on 5 mg BA per L (22.2 μM) combined with 0.2 mg 2,4-D per L (0.9 μM). To produce whole plants, shoots were separated and rooted on hormone-free medium containing 1 g activated charcoal per
L. Rachises provided an excellent source of explants for Epimedium micropropagation and proved suitable for callus production. 相似文献
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二十世纪我国植物学家对植物组织培养的贡献 总被引:7,自引:0,他引:7
朱至清 《Acta Botanica Sinica》2002,44(9):1075-1084
回顾了上一世纪我国植物组织培养的发展。 1934年以来 ,我国的植物组织培养研究一直与国际发展同步进行。我国学者在离体器官发生、茎尖培养、花药培养、子房培养、胚乳培养、原生质体培养和细胞大量培养等分支领域都取得重要进展。本文在引证我国研究者发表的植物组织培养论文的基础上 ,着重评述了那些被国际同行公认的研究成果。此外 ,还介绍了植物组织培养在我国农业和工业上应用的情况 相似文献
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朱至清 《植物学报(英文版)》2002,44(9):1075-1084
回顾了上一世纪我国植物组织培养的发展.1934年以来,我国的植物组织培养研究一直与国际发展同步进行.我国学者在离体器官发生、茎尖培养、花药培养、子房培养、胚乳培养、原生质体培养和细胞大量培养等分支领域都取得重要进展.本文在引证我国研究者发表的植物组织培养论文的基础上,着重评述了那些被国际同行公认的研究成果.此外,还介绍了植物组织培养在我国农业和工业上应用的情况. 相似文献
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吴柏艳;谢彦梅;王振月 《植物研究》2012,32(6):685-688
以毛脉酸模下胚轴和幼苗根茎为外植体,以MS为基本培养基,与不同浓度比例的6-BA和2,4-D等植物生长调节剂进行毛脉酸模组织培养的研究。结果表明,毛脉酸模幼苗根茎是诱导丛生芽的最佳材料,诱导丛生芽产生的最佳培养基为MS+6-BA 3.0 mg·L-1+2,4-D 0.1 mg·L-1,诱导率为81.2%;生根培养基为无激素的MS培养基,生根率可达92%,在此条件下生根迅速,可获得完整植株;组培苗经过炼苗移栽后成活率可达80%。 相似文献
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The phytotoxic effects of antibiotic treatment on micropropagated Clematis, Delphinium, Hosta, Iris and Photinia were determined by assessing multiplication and rooting rates of plants in vitro and weaning success and flowering in vivo.Abbreviations BA
6-benzyladenine
- IAA
3-indole-acetic acid
- NAA
1-naphthyl-acetic acid 相似文献
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蝴蝶兰的组织培养和快速繁殖 总被引:26,自引:0,他引:26
通过诱导残败花梗上的休眠芽萌发,以萌发的幼叶和去茎尖的茎段为外植体进行组织培养,建立了蝴蝶兰(Phalaenopsis amabilis Bl.)的无菌繁殖体系,并筛选出最佳培养基组成.诱导休眠芽萌发的最佳培养基为不加任何激素的MS0培养基;原球茎诱导的适宜培养基为MS 3.0 mg·L-1 6-BA 0.5 mg·L-1 ZT 30 mg·L-1柠檬酸和MS 5.0 mg·L-1 6-BA 30 mg·L-1柠檬酸 30%椰乳(CM),其中茎段的诱导效果明显优于叶片,诱导率达95%;诱导无菌苗生根的最适培养基为1/4 MS 1.0 mg·L-1 6-BA 0.1 mg·L-1 NAA,生根率可达79%. 相似文献
20.
4种红景天植物的组织培养研究 总被引:9,自引:1,他引:9
以大花红景天、云南红景天、长鞭红景天和库页红景天的茎和叶为外植体进行组织培养,结果表明:大花红景天以茎为外植体诱导芽效果最好,其它3种红景天以叶为外植体诱导芽效果最好。云南红景天和长鞭红景天适合的芽诱导激素组合是0.1mg/L NAA和2.5mg/L 6-BA的组合,在该激素水平下两种红景天的出芽频率分别达到71%和84%;大花红景天和库叶红景天适合的芽诱导激素组合是0.5mg/L NAA和2.5mg/L 6-BA的组合,在该激素水平下两种红景天的出芽频率均达到80%。长鞭红景天和库叶红景天在添加IBA的培养基上容易生根形成完整植株,生根率分别达到87%和73%;经过炼苗后,长鞭红景天再生苗能够成功移栽,成活率达66%。 相似文献