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Formamide polyacrylamide gel electrophoresis shows that chicken globin mRNA contains about 6.50 nucleotides, and since only 435 of these code for globin, a further 215 are not translated, and their function and position are not known. This work has produced the following conclusions. 1. 45-50 of these untranslated nucleotides are present as poly (A) at the 3' terminus. 2. The 3' untranslated region of chicken globin mRNA is at least 90 nucleotides in length. This minimal estimate is based on data derived from hybridization of defined lenghts of chicken globin cDNA to rabbit globin mRNA. The percentage of avian globin cDNA sequences which hybridize to rabbit globin mRNA is directly proportional to the length of the cDNA in each case. This relationship holds for lengths of cDNA from 115 up to 620 nucleotides. The low percentage homology for short cDNA molecules is not due to their being short per se. In homologous mRNA excess hybridizations (chicken cDNA/chicken mRNA), all cDNA preparations were completely protected from S1 nuclease digestion. 3. It is probable that there is greater evolutionary divergence in the 3' untranslated region of chicken and rabbit globin mRNA when compared with the coding regions of these molecules; The combined data is sued to formulate a regional map of chicken globin mRNA, 相似文献
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Characterization of synthetic rabbit globin DNA 总被引:4,自引:0,他引:4
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G N Buell M P Wickens F Payvar R T Schimke 《The Journal of biological chemistry》1978,253(7):2471-2482
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Enzymatic in vitro synthesis of globin genes. 总被引:74,自引:0,他引:74
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Characterization of an rRNA gene-specific cDNA probe: applications in bacterial identification 总被引:5,自引:0,他引:5
N Cox J Johnston Z Szarka D J Wright L C Archard 《Journal of general microbiology》1990,136(8):1639-1643
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Nucleotide sequence of 3'' untranslated portion of human alpha globin mRNA. 总被引:9,自引:4,他引:5
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J T Wilson J K deRiel B G Forget C A Marotta S M Weissman 《Nucleic acids research》1977,4(7):2353-2368
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