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FCA and FY are flowering time related genes involved in the autonomous flowering pathwayin Arabidopsis.FCA interacts with FY to regulate the alternative processing of FCA pre-mRNA.The FCA/FY interaction is also required for the regulation of FLC expression,a major floral repressor in Arabidopsis.However,it is not clear if the regulation of this autonomous flowering pathway is also present in monocotplants,such as rice.Recently,alternative RNA processing of OsFCA was observed in rice,which stronglysuggested the existence of an autonomous flowering pathway in rice.In this work,we cloned the cDNA ofthe autonomous flowering pathway gene OsFY from rice.The predicted OsFY protein contained a conserved7 WD-repeat region and at least two Pro-Pro-Leu-Pro motifs compared to Arabidopsis FY.The protein-protein interaction between OsFY and OsFCA-γ,the key feature of their gene function,was also demon-strated using the yeast two-hybrid system.The GenBank database search provided evidence of expressionfor other autonomous pathway gene homologs in rice.These results indicate that the autonomous floweringpathway is present in monocots,and the regulation through FY and FCA interaction is conserved betweenmonocots and dicots. 相似文献
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Krause CD Lavnikova N Xie J Mei E Mirochnitchenko OV Jia Y Hochstrasser RM Pestka S 《Cell research》2006,16(1):55-69
We previously demonstrated using noninvasive technologies that the interferon-gamma (IFN-γ) receptor complex is preassembled [ 1 ]. In this report we determined how the receptor complex is preassembled and how the ligand-mediated conformational changes occur. The interaction of Statl with IFN-γR1 results in a conformational change localized to IFN- γR1. Jakl but not Jak2 is required for the two chains of the IFN-γ receptor complex (IFN-γR1 and IFN-γR2) to interact; however, the presence of both Jakl and Jak2 is required to see any ligand-dependant conformational change. Two IFN- γR2 chains interact through species-specific determinants in their extracellular domains. Finally, these determinants also participate in the interaction of IFN-γR2 with IFN-γR1. These results agree with a detailed model of the IFN-γ receptor that requires the receptor chains to be pre-associated constitutively for the receptor to be active. 相似文献
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Kaempfer R 《Cell research》2006,16(2):148-153
PKR, the interferon (IFN)-inducible protein kinase activated by double-stranded RNA, inhibits translation by phosphorylating the initiation factor eIF2α chain. Uniquely, human IFN-γ mRNA uses local activation of PKR in the cell to control its own translation yield. IFN-γ mRNA activates PKR through a structure in its 5'- region harboring a pseudoknot which is critical for PKR activation. Mutations that impair pseudoknot stability reduce the ability of IFN-γ mRNA to activate PKR and strongly increase its translation efficiency. The cis-acting RNA element in IFN-γ mRNA functions as a biological sensor of intracellular PKR levels. During an immune response, as IFN-γ and other inflammatory cytokines build up in the cell's microenvironment, they act to induce higher levels of PKR in the cell, resulting in a more extensive activation of PKR by IFN-γ mRNA. With the resulting phosphorylation of eIF2α, a negative feedback loop is created and the production of IFN-γ is progressively attenuated. We propose that the therapeutic effect of IFN-β in multiple sclerosis may rest, at least in part, on its exquisite ability to induce high levels of PKR in the cell and thereby to limit IFN-γ mRNA translation through this negative feedback loop, blocking the excessive IFN-γ gene expression that precedes clinical attacks. 相似文献
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IFN-γ在沙眼衣原体感染的细胞培养中的影响 总被引:1,自引:0,他引:1
为了探讨IFN-γ在沙眼衣原体(chlamydia trachomatis,ct)感染细胞培养中的影响,本实验采用不同浓度γ-干扰素作用于ct感染的HeLa细胞,用透射电镜观察HeLa细胞内原体(elementary bodies,EBs)和网状体(reticulate bodies,RBs)。同时用反相高效液相色谱法(reversed-phase high performance liquid chromatography,HPLC)测定细胞内色氨酸的浓度。结果显示不同浓度γ-干扰素作用下细胞内ct的EBs和RBs形态及数量不同,中高浓度的IFN-γ作用下胞内EBs和RBs明显减少或轻度减少,et生长受到抑制,细胞内色氨酸含量与γ-干扰素量呈负相关,说明IFN-γ通过诱导产生2,3-吲哚-双加氧酶(indoleamine2,3-dioxygenase,IDO)降解色氨酸而抑制ct生长,提示γ-干扰素是抑制细胞内感染ct生长的一个重要因素。 相似文献
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白细胞介素 18(IL 18),是最近发现的一种新型免疫细胞调节因子,能显著刺激Th1细胞产生IFN γ,所以最初命名为IGIF(IFN γinducingfactor)。它与IL 12有协同作用,却不互相依赖。此外,它还能增强NK细胞和FasL介导的细胞毒效应,能促进内毒素引发的肝损伤。在肿瘤、自身免疫性疾病及抗炎症治疗中可能有潜在的应用前景 相似文献
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γ-干扰素信号通路及功能相关基因 总被引:1,自引:0,他引:1
γ-干扰素(IFN-γ)是细胞因子超家族中IFN家族的重要成员,具有抗病毒、抗肿瘤及免疫调节等多种生物学功能。IFN-γ在其效应细胞内多种信号转导途径的交互作用及其相关刺激基因的表达,导致了其生物学功能的复杂性和多样性。章对IFN-γ及其受体的结构和功能;IFN-γ介导的多条信号转导通路,以及IFN-γ刺激基因的生物学功能等作概述。 相似文献
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蛋白质定点PEG化研究:97Cys—INF—γ的巯基专一PEG修饰 总被引:4,自引:0,他引:4
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磷脂酶C-γ(PLC-γ)被激活后,通过两种不同的活化机制催化水解PIP2,产生IP3和DAG。IP3和DAG分别介导钙离子从钙泵中释放以及激活蛋白激酶C,从而形成一个关键的跨膜转导信号组分。现已知道,PLC-γ在细胞周期、细胞转化、生长和发育、细胞凋亡以及调控细胞肌动蛋白骨架等生命活动过程中起着重要的调节作用。另外,PLC-γ与其他转导信号,如PKC、Ras、Ca^2 等存在cross-talk关系。 相似文献
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激光—核辐射诱变小麦后代相关遗传力的分析 总被引:1,自引:0,他引:1
本试验采用He-Ne和N2激光辐照“汉原小麦”等四个材料的干种子,采用随机区组设计,重复三次,利用生物统计学和数量遗传学的方法,从个水平上对激光-核辐射小麦L2代抽穗期等19个性状的相关遗传力分析表明:利用株高间接选择结实小穗数、旗叶长、小穗密度和有效分蘖数。 相似文献
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信号分子磷脂酶C-γ(PLC-γ)被蛋白酪氨酸酶(PTK)激活催化水解磷脂酰肌醇4,5-二磷酸(PIP2)生成第二信使分子肌醇三磷酸(IP3)和二酰基甘油(DAG),参与受体酪氨酸激酶(RTK)介导的细胞分列、抗原与免疫细胞受体结合引起免疫反应及卵细胞受精等过程中的信号传递。 相似文献
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目的:评价Thl细胞因子IFN-γ在幽门螺杆菌(Hp)感染时对胃上皮细胞的作用。方法:胃上皮细胞经IFN-γ处理后,流式细胞术测定表面MHC-Ⅱ类分子的表达和Hp的黏附,ELISA法测定细胞因子对Hp致胃上皮细胞凋亡的影响。结果:IFN-γ可诱导胃上皮细胞表达MHCR类分子,进而增加Hp的黏附。IFN-γ本身即可诱导胃上皮细胞凋亡,并可促进Hp诱导的胃上皮凋亡。结论:Thl细胞因子IFN-γ参与并加剧了Hp感染所致的胃黏膜炎症。 相似文献
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中国鸭IFN—γ基因的克隆与表达 总被引:3,自引:0,他引:3
鸭乙型肝炎病毒(DHBV)感染鸭,是研究IFN-γ在机体自然感染过程中机体与病毒的相互作用和病毒的清除机制的良好动物模型。从PHA刺激后的鸭外周血单核细胞(PBMC)中提取RNA,通过RT-PCR获得鸭IFN-γ(DuIFN-γ)cDNA基因,构建DuIFN-γ真核表达质粒,转染COS-7细胞,经细胞病变效应(CPE)抑制分析和MTT法对重组DuIFN-γ滴度进行测定。实验表明,重组DuIFN-γ能够抑制VSV感染鸭胚成纤维细胞而产生的细胞病变效应。抗-DuIFN-γ抗体,能中和这种抗病毒活性。并且,GST-DuIFN-γ融合蛋白在大肠杆菌中得到表达和进一步纯化。 相似文献
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LIGHT sensitizes IFNγ-mediated apoptosis of HT-29 human carcinoma cells through both death receptor and mitochondria pathways 总被引:7,自引:0,他引:7
LIGHT [homologous to lymphotoxins, shows inducible expression, and competes with herpes simplex virus glycoprotein D for herpes virus entry mediator (HVEM/TR2)] is a new member of TNF superfamily. The HT-29 colon cancer cell line is the most sensitive to LIGHT-induced, IFNγ-mediated apoptosis among the cell lines we have examined so far. Besides downregulation of Bcl-XL, upregulation of Bak, and activation of both PARP [poly (ADP-ribose) polymerase] and DFF45 (DNA fragmentation factor), LIGHT-induced, IFNy-mediated apoptosis of HT-29 cells involves extensive caspase activation. Caspase-8 and caspase-9 activation, as shown by their cleavages appeared as early as 24 h after treatment, whereas caspase-3 and caspase-7 activation, as shown by their cleavages occurred after 72h of LIGHT treatment. Caspase-3 inhibitor Z-DEVD-FMK (benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone) and a broad range caspase inhibitor Z-VAD-FMK (benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone) were able to block LIGHT-induced, IFNγ-mediated apoptosis of HT-29 cells. The activity of caspase-3, which is one of the major executioner caspases, was found to be inhibited by both Z-DEVD-MFK and Z-VAD-FMK. These results suggest that LIGHT-induced, IFNy-mediated apoptosis of HT-29 cells is caspase-dependent, and LIGHT signaling is mediated through both death receptor and mitochondria pathways. 相似文献