Overcoming Obstacles to Evolution Education: The OOPSIE Compromise—A Big Mistake

Opt-out policies are typically invoked to excuse students from activities to which they or their parents may have religious objections, such as reciting the Pledge of Allegiance, dissecting animals in a laboratory, or attending sex education classes. Occasionally, however, a school or school district allows students to opt out of academic topics, including, sometimes, evolution. Opt-out policies specifically including evolution are a big mistake—for the students who opt out, for their classmates whose studies are disrupted, and especially for their teachers, who cannot fulfill their duty to instruct their charges about biology without emphasizing evolution.     

Self-disclosure with Dogs: Dog Owners’ and Non-dog Owners’ Willingness to Disclose Emotional Topics

Many owners talk to their pets about a wide range of issues, but there is very little research that has considered the content of this, or its impact on owner wellbeing. Verbal disclosure brings a range of potential health benefits, yet a number of factors may prevent individuals from confiding in their partners or friends (confidants). As such, in some circumstances, dogs may provide a more favorable alternative focus for disclosure. In a survey, we assessed dog owners’ (n = 286) and non-dog owners’ (n = 64) self-reported willingness to talk to their dog (dog owners only), their partner and their confidant. We used the Emotional Self Disclosure Scale (ESDS) for non-dog owners, and an adapted version of this for dog owners: Emotional Self Disclosure Scale–Dog Owners (ESDS-DO). Both dog owners and non-dog owners demonstrated a greater willingness to disclose to their partner than a confidant. For dog owners, their dog appeared to play a similar role as their partner, with greater willingness to talk to their dog about depression, jealousy, anxiety, calmness, apathy, and fear-related emotions, compared with a confidant. When talking about jealousy and apathy, dog owners reported greater willingness to talk to their dog than their partner or a confidant, but between-group comparisons (dog owner vs non-dog owner) revealed that dog owners and non-dog owners did not significantly differ in their willingness to talk to their partner or confidant, suggesting human relationships were not negatively affected by confiding to the dog. Participant age and length of relationship with their partner did not affect disclosure patterns for dog owners or non-dog owners. Males and females showed different willingness to disclose to confidants, but not to dogs. The results have implications for considering the value of dogs for human psychological health.     

The Color of My Skin: A Measure to Assess Children's Perceptions of Their Skin Color

The Color of My Skin is an instrument developed to assess children's internalized idea (abstraction) of the color of their skin; their satisfaction with that color; the desire, if any, to change the color of their skin; and their affect regarding their skin color. The assessment is part of a questionnaire utilized in a 3-year longitudinal study that examines psychosocial development, physical health, and behavioral adjustment of Puerto Rican children (N = 257) reared in the Greater Boston area. The results demonstrate that children's internalized representation of their skin color is a construct that can be reliably and validly measured. The children's ratings of their skin color were not associated with their sex, school grade, ethnic identity, the child's or the parent's nativity, or the racial or ethnic compositions of 3 social contexts: their neighborhood, their classmates, and their closest friends. Puerto Rican children did not show a preference for light-colored skin. Moreover, there were no significant differences in self-esteem based on the child's self-reported skin color. The lack of association between self-esteem and skin color was interpreted in light of a developmental tendency prevalent in early to middle childhood to place a positive value on different aspects of one's self. Whereas almost all children (96%) reported being happy or very happy with their color, 16% of the children would like to change their skin color if they could (51% to a lighter and 46% to a darker color).     

Phenolamides: Bridging polyamines to the phenolic metabolism

Phenolamides constitute a diverse and quantitatively major group of secondary metabolites resulting from the conjugation of a phenolic moiety with polyamines or with deaminated aromatic aminoacids. This review summarizes their bioactivities and their reported roles in plant development, adaptation and defence compared to those of their polyamine precursors. The most conclusive recent developments point to their contribution to cell-wall reinforcement and to direct toxicity for predators and pathogens, either as built-in or inducible defence. Phenolamides were often considered as accumulated end-chain products. Recent data bring a light on their biosynthesis and suggests their possible contribution in the branching of the phenylpropanoid metabolism.     

Plasticity in animal personality traits: does prior experience alter the degree of boldness?

Theoreticians predict that animal 'personality' traits may be maladaptive if fixed throughout different contexts, so the present study aimed to test whether these traits are fixed or plastic. Rainbow trout (Onchorhyncus mykiss) were given emboldening or negative experiences in the forms of watching bold or shy individuals responding to novelty or winning or losing fights to examine whether prior experience affected boldness. Bold individuals that lost fights or watched shy demonstrators became more shy by increasing their latency to approach a novel object, whereas shy observers that watched bold demonstrators remained cautious and did not modify their responses to novelty. Shy winners became bolder and decreased their latency to approach a novel object, but shy losers also displayed this shift. In comparison, control groups showed no change in behaviour. Bold fishes given negative experiences reduced their boldness which may be an adaptive response; however, shy fishes may base their strategic decisions upon self-assessment of their relative competitive ability and increase their boldness in situations where getting to resources more quickly ensures they outcompete better competitors.     

Fathers' Influence on Their Children's Cognitive and Emotional Development: From Toddlers to Pre-K

The Color of My Skin is an instrument developed to assess children's internalized idea (abstraction) of the color of their skin; their satisfaction with that color; the desire, if any, to change the color of their skin; and their affect regarding their skin color. The assessment is part of a questionnaire utilized in a 3-year longitudinal study that examines psychosocial development, physical health, and behavioral adjustment of Puerto Rican children (N = 257) reared in the Greater Boston area. The results demonstrate that children's internalized representation of their skin color is a construct that can be reliably and validly measured. The children's ratings of their skin color were not associated with their sex, school grade, ethnic identity, the child's or the parent's nativity, or the racial or ethnic compositions of 3 social contexts: their neighborhood, their classmates, and their closest friends. Puerto Rican children did not show a preference for light-colored skin. Moreover, there were no significant differences in self-esteem based on the child's self-reported skin color. The lack of association between self-esteem and skin color was interpreted in light of a developmental tendency prevalent in early to middle childhood to place a positive value on different aspects of one's self. Whereas almost all children (96%) reported being happy or very happy with their color, 16% of the children would like to change their skin color if they could (51% to a lighter and 46% to a darker color).     

Climate change and local public health in the United States: preparedness, programs and perceptions of local public health department directors

While climate change is inherently a global problem, its public health impacts will be experienced most acutely at the local and regional level, with some jurisdictions likely to be more burdened than others. The public health infrastructure in the U.S. is organized largely as an interlocking set of public agencies at the federal, state and local level, with lead responsibility for each city or county often residing at the local level. To understand how directors of local public health departments view and are responding to climate change as a public health issue, we conducted a telephone survey with 133 randomly selected local health department directors, representing a 61% response rate. A majority of respondents perceived climate change to be a problem in their jurisdiction, a problem they viewed as likely to become more common or severe over the next 20 years. Only a small minority of respondents, however, had yet made climate change adaptation or prevention a top priority for their health department. This discrepancy between problem recognition and programmatic responses may be due, in part, to several factors: most respondents felt personnel in their health department--and other key stakeholders in their community--had a lack of knowledge about climate change; relatively few respondents felt their own health department, their state health department, or the Centers for Disease Control and Prevention had the necessary expertise to help them create an effective mitigation or adaptation plan for their jurisdiction; and most respondents felt that their health department needed additional funding, staff and staff training to respond effectively to climate change. These data make clear that climate change adaptation and prevention are not currently major activities at most health departments, and that most, if not all, local health departments will require assistance in making this transition. We conclude by making the case that, through their words and actions, local health departments and their staff can and should play a role in alerting members of their community about the prospect of public health impacts from climate change in their jurisdiction.     

How mothers find their pups in a colony of Antarctic fur seals

In Antarctic fur seals, Arctocephalus gazella, mothers must identify their own young among hundreds or even thousands of pups, if they are to invest in their own offspring and avoid misdirecting their parental care. When returning to their breeding colony from a foraging trip of several days at sea, mothers have to find and identify their young before suckling can occur. There appears to be little confusion about which pup belongs to a mother, and adoption is absent or rare. Using behavioral observations, we investigated the means by which female Antarctic fur seals identified their pups in a breeding colony of about 750 mother-pup pairs on Kerguelen Island. We evaluated the importance of vision, scent communication, vocalizations, and rendezvous locations as possible explanations of how mothers find their pups. Every pup that a mother examined, whether her own or not, exchanged naso-nasal inspection with her, suggesting a strong role for olfactory communication in individual recognition. Both mothers and pups called to each other, and mothers that searched for pups over a longer period gave more calls and encountered more pups. Thus, vocalizations may have been used to attract pups that might be offspring. Nursing usually occurred in the same place from the end of one maternal visit to the colony and the arrival at the beginning of the next visit, suggesting that nursing locations may serve as a meeting place, or rendezvous, for mothers and pups. These results suggest that finding pups is a two-stage process for females, in which pups for sampling are attracted by calls or examined at the previous nursing location, and then individual identification is made by olfactory cues.     

Adhesion molecule signalling: not always a sticky business

The signalling activity of cell adhesion molecules (CAMs) such as cadherins, immunoglobulin-like CAMs or integrins has long been considered to be a direct consequence of their adhesive properties. However, there are physiological and pathological processes that reduce or even abrogate the adhesive properties of CAMs, such as cleavage, conformational changes, mutations and shedding. In some cases these 'adhesion deficient' CAMs still retain signalling properties through their cytoplasmic domains and/or their mutated or truncated extracellular domains. The ability of CAMs to activate signal transduction cascades in the absence of cell adhesion significantly extends their range of biological activities.     

Adaptation versus pleiotropy: why do males harm their mates?

Recent studies have documented male traits that cause physicalharm to their mates during copulation. Such harm has been suggestedto either (1) arise as a negative pleiotropic side effect ofadaptations that give males a reproductive advantage in anothercontext or (2) represent a male adaptation per se. In otherwords, male traits that cause harm to their mates may becomeestablished despite the fact that they cause harm or becausethey do so. A critical assumption of the latter hypotheses isthat females respond to infliction of harm in a manner thatis beneficial to their mates: by reducing their propensity toremate and/or by elevating their current reproductive rate.In the present study, we test this assumption by experimentallyinflicting various forms of harm to females immediately aftercopulation in three different insect species. We reveal thatfemales do not delay remating or increase their reproductiverate after being harmed but, on the contrary, remate soonerand lay fewer eggs in some cases. We conclude that selectionfor infliction of harm to females per se is unlikely. Instead,available empirical evidence supports the hypothesis that harmfulmale traits arise as negative pleiotropic side effects of adaptationsthat yield other selective advantages to males during reproductivecompetition.     

Dispersal in Marine Organisms without a Pelagic Larval Phase

In contrast to marine organisms whose offspring go through an extended planktonic stage, the young of others develop directly into benthic juveniles or into yolky nonfeeding larvae that spend only a few hours in the plankton before settling. Yet, paradoxically, many such species have geographic distributions that are comparable to those with a pelagic dispersal stage. This article reviews some of the ways in which these organisms can expand their distributions: drifting, rafting, hitchhiking, creeping, and hopping. Drifting applies to species in which larvae may be short-lived, but adults can detach or be detached from their benthic substratum and be passively carried to new areas, floating at the water's surface or below it. Many encrusting species and mobile species can spread by rafting, settling on natural or artificial floating substrata which are propelled by wind and currents to new regions. Hitchhiking applies to those attaching to vessels or being carried in ballast water of ships to a distant region in which their offspring can survive. Other marine species extend their distributions by hopping from one island of hard substratum or favorable sedimentary microhabitat to another, while creeping species extend their distributions along shores or shelves where habitats remain similar for long distances.     

Olfactory navigation to the nesting burrow in Leach's petrel (oceanodroma leucorrhoa)

Visual, auditory, and olfactory navigation in the homing behaviour of Leach's petrel to the nesting burrow were investigated. Petrels returning to their burrows at night hovered above the thick spruce-fir canopy in the vicinity of the burrow before plummeting to the forest floor a few metres downwind of their burrows. They then walked upwind to their burrows. Birds landed closer to, and followed more circuitous routes to, their burrows in still air than in a wind. They failed to avoid obstacles in the path to burrows, often failed to locate accurately burrow entrances on first trial, and vocalized only after entering the burrow. In a Y-maze olfactorium, captive breeding petrels chose an air current coming from their own nest material in preference to one from similar materials collected on the forest floor. In the same apparatus, birds did not respond positively to air currents from their own stomach oil or preen gland oil. Petrels taken from burrows and released that same night did not return within a week if their external nares were plugged or if their olfactory nerves were transected. They did return if not operated on or if only subjected to sham operations. These results support an olfactory guidance system in burrow location and argue against visual or auditory guidance.     

Molecular motors: how to make models that can be used to convey the concept of molecular ratchets and thermal capture

A wide variety of cellular processes use molecular motors, including processive motors that move along some form of track (e.g., myosin with actin, kinesin or dynein with tubulin) and polymerases that move along a template (e.g., DNA and RNA polymerases, ribosomes). In trying to understand how these molecular motors actually move, many apply their understanding of how man-made motors work: the latter use some form of energy to exert a force or torque on its load. However, quite a different mechanism has been proposed to possibly account for the movement of molecular motors. Rather than hydrolyzing ATP to push or pull their load, they might use their own thermal vibrational energy as well as that of their load and their environment to move the load, capturing those movements that occur along a desired vector or axis and resisting others; ATP hydrolysis is required to make backward movements impossible. This intriguing thermal capture or Brownian ratchet model is relatively more difficult to convey to students. In this report, we describe several teaching aids that are very easily constructed using widely available household materials to convey the concept of a molecular ratchet.     

The Emotional Lives of Companion Animals: Attachment and Subjective Claims by Owners of Cats and Dogs

There is a growing body of scientific evidence supporting the existence of emotions in nonhuman animals. Companion-animal owners show a strong connection and attachment to their animals and readily assign emotions to them. In this paper we present information on how the attachment level of companion-animal owners correlates with their attribution of emotions to their companion cat or dog and their attribution of mirrored emotions. The results of an online questionnaire, completed by 1,023 Dutch-speaking cat and/or dog owners (mainly in the Netherlands and Belgium), suggest that owners attribute several emotions to their pets. Respondents attributed all posited basic (anger, joy [happiness], fear, surprise, disgust, and sadness) and complex (shame, jealousy, disappointment, and compassion) emotions to their companion animals, with a general trend toward basic emotions (with the exception of sadness) being more commonly attributed than complex emotions. All pet owners showed strong attachment to their companion animal(s), with the degree of attachment (of both cat and dog owners) varying significantly with education level and gender. Owners who ascribed human characteristics to their dog or cat also scored higher on the Pet Bonding Scale (PBS). Finally, owners who found it pleasant to pet their dog or cat had a higher average PBS score than those who did not like to do so. The relationship between owners’ attributions of mirrored emotions and the degree of attachment to dogs was significant for all emotions, whilst for cats this relationship was significant only for joy, sadness, surprise, shame, disappointment, and compassion.     

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Elastin-like polypeptides are repetitive biopolymers that exhibit a lower critical solution temperature phase transition behavior, existing as soluble unimers below a characteristic transition temperature and aggregating into micron-scale coacervates above their transition temperature. The design of elastin-like polypeptides at the genetic level permits precise control of their sequence and length, which dictates their thermal properties. Elastin-like polypeptides are used in a variety of applications including biosensing, tissue engineering, and drug delivery, where the transition temperature and biopolymer architecture of the ELP can be tuned for the specific application of interest. Furthermore, the lower critical solution temperature phase transition behavior of elastin-like polypeptides allows their purification by their thermal response, such that their selective coacervation and resolubilization allows the removal of both soluble and insoluble contaminants following expression in Escherichia coli. This approach can be used for the purification of elastin-like polypeptides alone or as a purification tool for peptide or protein fusions where recombinant peptides or proteins genetically appended to elastin-like polypeptide tags can be purified without chromatography. This protocol describes the purification of elastin-like polypeptides and their peptide or protein fusions and discusses basic characterization techniques to assess the thermal behavior of pure elastin-like polypeptide products.     

小菜蛾经历"保卫德"后产卵选择行为的变化

植食性昆虫成虫对非寄主植物源驱避素的经历,可导致其对这类化合物产生习惯性反应或诱导嗜好性.让小菜蛾雌蛾对源于印楝的驱避剂——“保卫德”(BIOACT-T^TM EC508)经历2～6次或2～6日后,测定它们对经过保卫德处理的白菜植株的产卵选择行为.结果表明,保卫德对2日龄、4日龄、6日龄无经历雌蛾均有很强的驱避作用,它们在经过保卫德处理的白菜植株上的相对产卵率均只有10%左右;对保卫德有2次或2 d经历的2日龄雌蛾,该产卵率平均上升到17%;对保卫德有4次或4 d经历的4日龄雌蛾,该产卵率上升到21%～24%;对保卫德有6次或6 d经历的6日龄雌蛾,该产卵率上升到29%.表明经历可导致雌蛾对保卫德产生习惯性反应或诱导嗜好性,使保卫德的驱避作用下降,但这种经历导致的产卵选择行为变化在个体之间存在较大差异.     

Reglucosylation by UDP-glucose:glycoprotein glucosyltransferase 1 delays glycoprotein secretion but not degradation

UDP-glucose:glycoprotein glucosyltransferase 1 (UGT1) is a central quality control gatekeeper in the mammalian endoplasmic reticulum (ER). The reglucosylation of glycoproteins supports their rebinding to the carbohydrate-binding ER molecular chaperones calnexin and calreticulin. A cell-based reglucosylation assay was used to investigate the role of UGT1 in ER protein surveillance or the quality control process. UGT1 was found to modify wild-type proteins or proteins that are expected to eventually traffic out of the ER through the secretory pathway. Trapping of reglucosylated wild-type substrates in their monoglucosylated state delayed their secretion. Whereas terminally misfolded substrates or off-pathway proteins were most efficiently reglucosylated by UGT1, the trapping of these mutant substrates in their reglucosylated or monoglucosylated state did not delay their degradation by the ER-associated degradation pathway. This indicated that monoglucosylated mutant proteins were actively extracted from the calnexin/calreticulin binding-reglucosylation cycle for degradation. Therefore trapping proteins in their monoglucosylated state was sufficient to delay their exit to the Golgi but had no effect on their rate of degradation, suggesting that the degradation selection process progressed in a dominant manner that was independent of reglucosylation and the glucose-containing A-branch on the substrate glycans.     

Experimental evidence for costs due to chewing lice in the European bee-eater (Merops apiaster)

Animals frequently host organisms on their surface which can be beneficial, have no effect or a negative effect on their host. Ectoparasites, by definition, are those which incur costs to their host, but these costs may vary. Examples of avian ectoparasites are chewing lice which feed exclusively on dead feather or skin material; therefore, costs to their bird hosts are generally considered small. Theoretically, many possible proximate effects exist, like loss of tissue or food, infected bites, transmission of microparasitic diseases or reduced body insulation due to loss of feathers, which may ultimately also have fitness consequences. Here, we experimentally examined a possible negative impact of 2 feather-eating louse species (Meropoecus meropis and Brueelia apiastri) on male and female European bee-eaters (Merops apiaster) by removing or increasing louse loads and comparing their impact to a control group (lice removed and immediately returned) after 1 month. A negative effect of chewing lice was found on body mass and sedimentation rate and to a lesser extent on haematocrit levels. Males and females lost more weight when bearing heavy louse loads, and were more susceptible to infestations as indicated by the higher sedimentation rate. Our results further suggest differences in sex-specific susceptibility.     

Natal philopatry and recruitment of willow ptarmigan in north central and northwestern Canada

Summary Natal philopatry and recruitment were measured in two populations of willow ptarmigan; one near Churchill, Manitoba and the other in northwestern British Columbia. We examined the return of tagged offspring in subsequent years with respect to geographical area, annual variation, their age when tagged, their sex, their body weight, age and number of their parents, and time of hatch (first nest or renest). Most chicks were tagged before they fledged, but chicks tagged after that had the highest rate of return. We also observed a strong positive relationship between fledging success of broods and offspring return in following years.Patterns of offspring return were similar in both populations except that male offspring in Manitoba settled closer to their natal sites than those in British Columbia and more yearling captured in Manitoba had been tagged as chicks. Return of offspring did not vary with year, their body weights shortly after hatch, or with the age or number of parents raising them. However, a significantly higher proportion of offspring hatched from first nests (first-initiated clutches) returned compared to those hatched from renests (replacement clutches). The low return of chicks hatched from renests may due to low survival, low philopatry, or both. We observed no differences in the mating status (recruitment) of returning offspring with respect to the time they hatched or the number of parents that raised them.