Influence of bacteria and salinity on diatom biogenic silica dissolution in estuarine systems

Dissolution of diatom biogenic silica (bSiO₂) in estuaries and its control by water salinity and bacteria were investigated using the river euryhaline species Cyclotella meneghiniana as a model. Laboratory-controlled bioassays conducted at different salinities with an estuarine bacteria inoculum showed a faster dissolution of diatom bSiO₂ at the lowest salinity where bacteria were the most abundant. However in another experiment, salinity increase clearly enhanced the dissolution of cleaned frustules (organic matter free). The presence of active bacteria might therefore predominate on the effect of salinity for freshly lysed diatoms whereas salinity might rather control dissolution of organic-matter-free frustule remains. Incubation of cultivated diatoms at different protease concentrations revealed that high proteolytic activities had little effect on bSiO₂ dissolution at a 1-month scale in spite of an efficient removal of organic matter from the frustules. Altogether it is hypothesized that bacterial colonization increases bSiO₂ dissolution by creating a microenvironment at the diatom surface with high ectoproteolytic activity but also via the release of metabolic byproducts since the presence of organic matter seems generally to facilitate diatom bSiO₂ dissolution.     

Development of a diatom-based transfer function for lakes and streams severely impacted by secondary salinity in the south-west region of Western Australia

The association of diatom assemblages to salinity was studied in 95 lakes and streams ranging from freshwater to hypersaline in the south-west of Western Australia. The relationship between environmental variables and species composition was explored using canonical correspondence analysis (CCA) and partial CCA. Salinity was shown to account for a significant and independent amount of variation in the diatom data, enabling a transfer function to be developed based on the final dataset, which consisted of 89 sites and 150 diatom taxa. The most successful model was derived using tolerance-downweighted weighted averaging. Summary statistics showed that the transfer function performed very well with a high coefficient of determination and low prediction errors that remained high after the cross-validation method of jackknifing (r _apparent² = 0.97 and r _jackknifed² = 0.89). This suggests that salinity can be accurately predicted using relative abundances of diatoms, and the model can now be applied to paleolimnological reconstructions. However, the transfer function also provides the basis for use in future biomonitoring studies to detect increases in salinity for lakes and streams most at risk, as well as to evaluate the success of remediation measures implemented to secondary salinised systems. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Guest Editors: J. John & B. Timms Salt Lake Research: Biodiversity and Conservation—Selected papers from the 9th Conference of the International Society for Salt Lake Research     

The Holocene history of vegetation and settlement at the coastal site of Lake Voulkaria in Acarnania, western Greece

A palynological investigation of a Holocene profile from Lake Voulkaria, western Greece, was carried out as a contribution to the environmental history of the coastal area of northwestern Acarnania and the Classical city of Palairos. It shows that deciduous oaks dominated the natural vegetation of the area throughout the Holocene. Until ca. 7000 B.C. Pistacia occurred abundantly, while other evergreen woody taxa were rare. At ca. 6300 B.C. an expansion of Carpinus orientalis/Ostrya can be observed. Around ca. 5300 B.C. spreading of Erica indicates a change to a drier climate and/or first human impact. Since ca. 3500 B.C. an increase of evergreen shrubs now clearly indicates land-use. The foundation of the Classical city of Palairos led to a temporary expansion of Phillyrea maquis. Within this period, molluscs of brackish water indicate the use of the lake as a harbour after the construction of a connection to the sea. The deciduous Quercus woodland recovered when human impact decreased in the area, and lasted until modern times.     

Home range, movement, and site fidelity of translocated northern bobwhite (Colinus virginianus) in southwest Georgia, USA

During 1997 and 1998, we compared home range, movement, and site fidelity characteristics of translocated wild northern bobwhite (Colinus virginianus) to resident birds using radiotelemetry. We captured wild bobwhites (n=74) in southwest Georgia, USA just before the breeding season and relocated them (>1.6 km from capture sites) to sites nearby where previous density estimates revealed that populations were low compared to surrounding areas. Translocated birds were equipped with radiotransmitters and released in groups of 8 to 12. Resident birds (n=166) were also captured and simultaneously monitored via radiotelemetry. We found no difference in home range size (F ₁=0.08, P=0.78), mean daily movements (F ₁=0.04, P=0.84), or distance moved from trap or release sites to arithmetic centers of home ranges (F ₁=1.58, P=0.21) between translocated and resident bobwhites. These results suggest that translocating wild bobwhites over relatively short distances into suitable habitat does not negatively influence bobwhite movement and renders site fidelity as reasonable. Therefore, translocation of wild bobwhites before breeding season can result in enhanced numbers of adult breeders in a target location and potentially augments fall populations via reproductive yield.     

Nest site selection and nesting success of five species of passerines in a South American open Prosopis woodland

I analyzed nest site preferences and whether these preferences were linked to nest success of five bird species breeding in an arid area of southern South America. Most nests (90%) were located in three plant species (Geoffroea decorticans, Capparis atamisquea, and Atriplex lampa). Serpophaga griseiceps, Poospiza ornata, and P. torquata nested in plants with size significantly different from randomly selected plants. At the mesohabitat scale (i.e., habitat patch surrounding the nest), four species showed clear differences from random patches within the general habitat, and the remaining species (Saltatricula multicolor) showed a non-significant trend. The daily survival rate of S. griseiceps nests placed in the preferred plant species (i.e., G. decorticans) was lower than those in the other nest-plants. In the other four bird species, nest survival did not differ between preferred and other plants used for nesting. At the scale of microhabitat (i.e., plant size and location of the nest inside the plant), no significant differences were detected between unsuccessful and successful nests. Similarly, unsuccessful nest patches did not differ significantly in habitat features from those that were successful. High nest predation rates found in this habitat (around 85% for the total assemblage) and a relatively high diversity of predators (and of predation tactics) appear to impede the presence of safe sites for nesting (i.e., there are trade-offs between avoidance strategies).     

Late holocene human impact on two coastal environments in New South Wales,Australia: a comparison of Aboriginal and European impacts

There are few historical analyses quantifying impacts of human activity in Australia. This paper compares vegetation change, fire regime, erosion and eutrophication rates between the European period and the recent prehistoric past in two lake systems on the south coast of New South Wales. The variance in pollen abundance and hence species population changes increased markedly in the historical period, especially amongst understorey taxa, and this could be related to changes in the local fire regimes and to the effects of grazing. Local fire activity decreased from the prehistorical period at both sites. Erosion rates increased in the historical period and both organic and inorganic components were deposited in the lakes. Erosion episodes could be related to fire during some periods but are clearly controlled by forest disturbance and land-use at other periods. The trophic status of the lakes was increasing from before European settlement but accelerated in the recent past. This was in part due to the increased erosion rates and in part due to fertiliser application. The results suggest that lower rates of erosional and eutrophic change occur in catchments with basaltic than with Holocene sand substrata.     

Vegetation development at a mountain settlement site in the Swedish Scandes during the late Holocene: palaeoecological evidence of human-induced deforestation

A palaeoecological study was conducted close to the forest limit in the northern Scandinavian mountain range. The aim was to elucidate the degree to which human impact has affected the vegetation at Hiednikvalta, a Stállo settlement site. Stállo settlements consist of round hut foundations that have a hearth in the middle and are surrounded by a low turf wall. They were probably established by Sámi people using the mountain areas for hunting and/or reindeer herding. In order to separate the effects of humans and climate on the vegetation, a reference area approach was adopted, i.e. the vegetation development at the Stállo settlement site Hiednikvalta was compared with the vegetation development in a forested reference area Avvuhatjåhkkå, at the same altitude as Hiednikvalta but with no archaeological remains of settlements. Peat stratigraphies were retrieved at the two sites and pollen analysis, loss-on-ignition (LOI), pollen accumulation rates (PAR), macrofossil analysis and Betula pollen size statistics were all examined. The results indicate that Hiednikvalta was forested with Betula trees prior to the Stállo settlement period, which occurred between the eighth and thirteenth centuries. Human activities resulted in a decrease in tree cover at the site, as found in a previous study at Adamvalta, another Stállo settlement site in the region. However, the magnitude of vegetation change, and the post-Stállo vegetation development differed between the two areas, suggesting that site-specific factors are important. The use of reference areas in palynological studies is also discussed.     

Macroinfauna community structure and biochemical composition of sedimentary organic matter along a gradient of wave exposure in sandy beaches (NW Spain)

Six sandy beaches on the North West coast of Spain, exposed to different wave action, were sampled in order to study the macroinfauna community and the biopolymeric fraction (proteins, lipids and carbohydrates) of sedimentary organic matter. According to McLachlan’s rating system (1980), three of them were classified as sheltered and the other three as exposed beaches. Sampling was carried out during August 2004 at three tidal levels: high, medium and low. Macroinfauna community and organic matter concentrations were found to be significantly different when sheltered and exposed beaches were compared. Macroinfauna diversity (H′), abundances and biomass became increasingly enriched along a gradient from exposed to sheltered beaches. Macroinfauna mean abundance was found higher in sheltered (ranked from 1535 ± 358 to 15062 ± 5771 ind m⁻²) than in exposed beaches (from 150 ± 41 to 5518 ± 1986 ind m⁻²). Macroinfauna biomass ranged from 3.2 to 14.7 g m⁻² and species richness from 25 to 27 in sheltered localities; while in exposed beaches, biomass ranged from 0.2 to 2.3 g m⁻² and the number of species from 5 to 14. The biopolymeric carbon concentration (BPC) was significantly higher in sheltered (from 84.7 ± 44.7 to 163.3 ± 34.8) than in exposed (from 30.3 ± 7.5 to 78.7 ± 12.3) beaches. The low hydrodynamic conditions of sheltered beaches favoured the settlement of organic rich fine sediments, being supported by the higher protein to carbohydrate ratio found in the exposed (from 23.5 ± 0.9 to 32.7 ± 4.4), rather than in the sheltered localities (from 6.2 ± 0.7 to 13.6). Mean macroinfauna abundances were higher at medium and low tidal levels in both sheltered and exposed beaches. Crustacea was found to be the main group inhabiting the upper part of both types of beaches, dominating all tidal levels of exposed sandy beaches. Mollusca and Polychaeta groups were dominant in sheltered beaches at the medium and lower levels. There was a significant negative relationship between the BPC and the beach face slope; thus, BPC decreased as the intertidal slope increased. It seems that exposed sandy beaches are mainly physically controlled, whereas hospitable sheltered beaches let other factors, such as biochemical compounds, enrich the benthic fauna scenery. Electronic Supplementary Material  Supplementary material is available in the online version of this article at and is accessible for authorized users Handling editors: K. Martens     

Wood properties of juvenile Pinus radiata growing in the presence and absence of competing understorey vegetation at a dryland site

Pinus radiata D. Don trees were grown in the presence and absence of the woody weed broom (Cytisus scoparius L.) on a dryland site for 2 years to determine the effects of competition from weeds on wood properties in juvenile trees. Wood property measurements made on cross-sections from the bark to the pith were scaled to convert results from distance to a time basis using sigmoidal equations fitted to monthly measurements of tree diameter. When averaged across the 2 years, the presence of the weeds significantly increased wood density (+11%), wall thickness (+6%) and modulus of elasticity (MOE_SS, +93%), and significantly reduced microfibril angle (MFA, –21%) and radial diameter (–8%). Radial growth rate was significantly correlated to wood density, and this relationship held across both treatment and age. At the seasonal scale, there was close correspondence between changes in MFA and growth rate. Ring width was significantly related to both MFA and MOE_SS at the annual scale. Although both of these relationships held across treatments, year significantly influenced the value of coefficients in the relationships. The results highlight the direct effects of the presence of weeds on wood properties and the need to consider silvicultural treatments appropriate for balancing gains in productivity with losses in wood quality for timber production.     

Life history costs and benefits of encephalization: a comparative test using data from long-term studies of primates in the wild

The correlation between brain size and life history has been investigated in many previous studies, and several viable explanations have been proposed. However, the results of these studies are often at odds, causing uncertainties about whether these two character complexes underwent correlated evolution. These disparities could arise from the mixture of wild and captive values in the datasets, potentially obscuring real relationships, and from differences in the methods of controlling for phylogenetic non independence of species values. This paper seeks to resolve these difficulties by (1) proposing an overarching hypothesis that encompasses many of the previously proposed hypotheses, and (2) testing the predictions of this hypothesis using rigorously compiled data and utilizing multiple methods of analysis. We hypothesize that the adaptive benefit of increased encephalization is an increase in reproductive lifespan or efficiency, which must be sufficient to outweigh the costs due to growing and maturing the larger brain. These costs and benefits are directly reflected in the length of life history stages. We tested this hypothesis on a wide range of primate species. Our results demonstrate that encephalization is significantly correlated with prolongation of all stages of developmental life history except the lactational period, and is significantly correlated with an extension of the reproductive lifespan. These results support the contention that the link between brain size and life history is caused by a balance between the costs of growing a brain and the survival benefits the brain provides. Thus, our results suggest that the evolution of prolonged life history during human evolution is caused by increased encephalization.     

Short and long-term effects of unguided internet-based cognitive behavioral therapy for chronic insomnia in morning and evening persons: a post-hoc analysis

ABSTRACT

A post-hoc analysis comparing morning and evening persons with insomnia on sleep and mental health characteristics was conducted in order to investigate whether an Internet-based cognitive behavioral therapy for insomnia (ICBTi) was effective both for morning and evening persons. Adult patients (N = 178, mean age = 44.8, 67% females) with insomnia were randomized to either ICBTi (N = 92; morning persons = 41; evening persons = 51) or a web-based patient education condition (N = 86; morning persons = 44; evening persons = 42). All patients were assessed with sleep diaries, the Insomnia Severity Index (ISI), the Bergen Insomnia Scale (BIS), the Dysfunctional Beliefs and Attitudes about Sleep Scale (DBAS-16), the Hospital Anxiety and Depression Scale (HADS) and the Chalder Fatigue Scale (CFQ). Patients were characterized as morning or evening persons based on a median split on the Horne-Östberg Morningness Eveningness Questionnaire. Short and long-term effects of treatment were examined with mixed-model repeated-measures analyses. Morning and evening persons did not differ in terms of age, gender or educational status. At baseline, morning persons had more wake time after sleep onset (d= 0.54, p < .001) and more early morning awakening (d= 0.38, p < .05) compared to evening persons, while evening persons reported longer sleep onset latency (d= 0.60, p < .001), more time in bed (d= 0.56, p < .001), longer total sleep time (d= 0.45, p < .01), more fatigue (d= 0.31, p < .05) and more dysfunctional beliefs and attitudes about sleep (d= 0.47, p < .01). Despite these differences at baseline, both morning and evening persons receiving ICBTi benefitted more across most measures compared to morning and evening persons who received patient education. For morning persons in the ICBTi group, ISI scores were reduced from 17.3 at baseline to 8.8 (d_pre-post = 2.48, p < .001) at post-assessment, and to 10.0 at 18-month follow up (d_pre-post18m = 2.13, p < .001). Comparable results were found for evening persons in the ICBTi group, with a reduction in ISI scores from 17.4 at baseline to 8.6 (d_pre-post = 2.24, p < .001) at post-assessment, and to 8.7 at 18-month follow up (d_pre-post18m = 2.19, p < .001). Similar results were found on the BIS, DBAS, HADS, CFQ and sleep diary data. Despite different insomnia symptomatology between the two groups, the current study suggests that ICBTi is effective across scores on the morningness-eveningness dimension. The study was pre-registered at: ClinicalTrials.gov Identifier: NCT02261272.     

Multiproxy study of anthropogenic and climatic changes in the last two millennia from a small mire in central Poland

The Żabieniec kettle hole is the first peatland in central Poland analyzed quantitatively with four biotic proxies (plant macrofossils, pollen, testate amoebae and chironomids) to reconstruct the past environmental change. Palaeoecological data were supported by historical and archaeological records. We focused on autogenic vegetation change and human impact in relation to climatic effects. The aims of our study were (a) to describe the development history of the mire during the last 2,000 years, (b) to date and reconstruct the anthropogenic land-use changes and (c) to discuss a possible climatic signal in the peat archive. The combination of proxies revealed dramatic shifts that took place in the peatland since the Roman Period. Żabieniec was a very wet telmatic habitat until ca. AD 600. Then, the water table declined, and the site transformed into a Sphagnum-dominated mire. This dry shift took place mainly during the Early Medieval Period. Human impact was gradually increasing, and it was particularly emphasized by deforestation since AD 1250 (beginning of the Late Medieval Period). Consequently, surface run-off and aeolian transport from the exposed soils caused the eutrophication of the mire. Furthermore, chironomids and testate amoebae reveal the beginning of a wet shift ca. AD 1350. Openness considerably increased in the Late Medieval and the Modern Periods. The highest water table during the last 1,000 years was recorded between AD 1500 and 1800. This wet event is connected with deforestation, but it could be also associated with the Little Ice Age. Our study shows plant succession in the Żabieniec peatland, which can be explained with the recent landscape transformation. However, such changes are also possibly linked with the major climatic episodes during the last two millennia, such as the Medieval Warm Period and the Little Ice Age. Guest editors: K. Buczkó, J. Korponai, J. Padisák & S. W. Starratt Palaeolimnological Proxies as Tools of Environmental Reconstruction in Fresh Water     

Effects of ethynylestradiol on vitellogenin synthesis and sex differentiation in juvenile grey mullet (Mugil cephalus) persist after long-term exposure to a clean environment

We investigated the continuing effects of exposure to ethynylestradiol (EE₂) in juvenile grey mullet after transfer to a clean environment. Eleven-month-old juvenile fish containing immature phenotype gonad were fed dry diets; the low and high EE₂-treated groups were fed diets with 0.04 and 4 μg EE₂/g body weight for 4 weeks, respectively. After treatment, they were transferred to clean seawater, and reared with an EE₂ free diet for 350 days. Vitellogenin (VTG) was not detected in the serum of the control group throughout the experimental period. However, in both treatment groups, abnormal values of serum VTG were detected until approximately 100 days after transfer to a clean environment. In the control group, sex differentiation was not confirmed until 206 days after transfer to a clean environment. However, some of the fish in the 0.04 μg EE₂-treated group had ovarian cavity and oocytes at 26 days. In most of the fish in the 4 μg EE₂-treated group, the ovarian cavity had already appeared at the end of EE₂ treatment (0 day), and oocytes were observed at 26 days, suggesting that EE₂ accelerates ovarian differentiation. These results suggest that previous exposure to EE₂ has long-term effects on VTG synthesis and gonadal development.     

森林与空旷地空气温湿度及土壤温度的长期对比研究

森林对温湿度的影响是其生态功能的基础,为了探讨森林的这种小气候效应,本文采用小气候对比观测的方法,根据2005 -2007年观测资料,对长白山阔叶红松林与附近空旷地温度、湿度等小气候要素进行了差异性研究.结果表明,林内近地表层空气温度白天低于林外空旷地,晚间高于林外空旷地,因而具有较低的日较差.非生长季二者的月平均值差异不显著,但生长季差异明显,月平均气温最高差值出现季节与森林叶面积指数最大值出现时间一致.气温年较差的平均值林内小于空旷地,差值可达6.3℃.年均森林与空旷地土壤温度全年均表现出明显差异,以0℃为界,0℃以上,林内土壤温度低于空旷地,0℃以下高于空旷地,其中2005年1月5 cm深处土壤温度差值达到了5.3℃.空气相对湿度生长季差异较大,其中以7、8月份差异最为明显,2006年7月差值最大,达7.0％.