共查询到20条相似文献,搜索用时 15 毫秒
1.
Effects of various combinations of nutrient solution salinity (0.3, 171, and 342 mM NaCl), photosynthetically active radiation (PAR) of 600 or 1150 μmol/(m 2 s), and type of nitrogen nutrition (amide-N or nitrate-N) on the productivity and the content of accumulated mineral nutrients and free amino acids were studied in Salicornia europaea plants. At PAR of 600 μmol/(m 2 s), plant productivity increased with elevation of salinity level; at 1150 μmol/(m 2 s), the maximum productivity was observed in the plants grown at 171 mM of NaCl. The content of free amino acids in shoots, regardless of PAR, decreased with growing salinity level, whereas Na content, on the opposite, increased. Glutamic acid, rather than proline, was shown to be the main organic osmolyte in this plant species. Comparison of the productivity of plants grown on solutions with amide (urea) or nitrate nitrogen showed that higher biomass accumulation was achieved in the former case. 相似文献
2.
Plant productivity of a common glasswort Salicornia europaea L. was investigated in relation to the type of nitrogen nutrition and as a function of macronutrient concentrations mimicking the mineral composition of human urine. The source of nutrient nitrogen had no substantial effect on productivity of Salicornia europaea. In plants grown on media with amide as a nitrogen source, the content of nitrogenous substances, including glutamic and aspartic amino acids, was higher than in plants grown with nitrate. In plants grown on media with mineral composition analogous to that of human urine, the shoots accumulated Na and K in almost equally high amounts, on the background of high and nearly equal Na and K concentrations in the nutrient media. 相似文献
3.
The role of calcium in the salt relations of the bean plant, Phaseolus vulgaris, was examined. Brittle wax bush bean plants were cultured in nutrient solutions containing 50 m M NaCl. In the absence of added calcium the plants showed a general breakdown of the roots. A low concentration of calcium in the nutrient solution (0.1 mM) prevented this. Without added calcium the plants absorbed and translocated sodium at such a rate that high concentrations of it built up in the leaves within two days. With increasing concentrations of calcium in the nutrient solution the leaves contained progressively less sodium, and at 3 m M CaSO 4 the concentrations of sodium in the leaves was equal to that of the control plants grown without addition of salt. Even after both roots and stems had reached a high concentration of sodium, the leaves of plants grown in the presence of adequate concentrations of calcium contained little sodium. 相似文献
4.
The effect of different N supply on amino acid export to the phloem was studied in young plants of wheat ( Triticum aestivum L. cv. Klein Chamaco), using the exudation in EDTA technique. Plants were grown in a growth cabinet in pots with sand, and supplied with nutrient solutions of different NO 3? concentrations. When plants were grown for 15 days with nutrient solutions containing 1.0, 3.0, 5.0, 10.0, 15.0 or 20.0 m M KNO 3, the exudation rate of sugars from the phloem was unaffected by N supply, but sugars accumulated in the leaf tissue when the N supply was limiting for growth. On the other hand, the rate of exudation of amino acids was proportional to the NO 3? concentration in the nutrient solution. When the supply of N to plants grown for 15 days with 15.0 m M NO 3? was interrupted, the exudation of sugars was again unaffected, but there was a fast decrease in the amount of amino acids exudated, and of the concentration of amino acids and nitrogen in the tissues. Also, when 10-day-old plants grown without N were supplied with 15.0 m M NO 3?, there was a sharp increase in the exudation of amino acids, without changes in the amount of sugar exudated. The rate of exudation of amino acids to the phloem was independent of the concentration of free amino acids in the leaves in all three types of experiment. Asp was the most abundant amino acid in the leaf tissue, while Glu was the one most abundant in the phloem exudate. Asp and Ala were exported to the phloem at a rate lower than expected from their leaf tissue concentrations, indicating some discrimination. On the contrary, Glu showed a preferential export at low N supply. It is concluded that the rate of amino acid export from the leaf to the phloem is dependent on the N available to the plant. This N is used for synthesis of leaf protein when the supply is low, exported to the phloem when supply is adequate, and accumulated in the storage pool when supply is above plant demand. 相似文献
5.
Four ecotypes of the species Lycopersicon cheesmanii ssp. minor (Hook.) C.H. Mull. from the Galapagos Islands were compared with L. esculentum Mill cv. VF 36 with respect to salt tolerance. The L. cheesmanii ecotype that proved most salt-tolerant was selected for detailed comparison with the L. esculentum cultivar. Plants were grown in modified Hoagland solution salinized with synthetic seawater salt mix. Growth rates under saline conditions were examined and amino acid, sugar, total amino nitrogen, free acidity, and Na and K levels in the tissues of the most and least tolerant plants were measured under salt stress and nonstress conditions. Results indicate that all Galapagos ecotypes were far more salt-tolerant than was the esculentum cultivar. They could survive in full strength seawater nutrient solution while the esculentum cultivar could not in most cases withstand levels higher than 50% seawater. Growth rates were reduced in both species under saline conditions but the esculentum cultivar was more severely affected. High levels of total amino nitrogen, specific amino acids, and free acidity along with low sodium content were found in the salt stressed VF 36 cultivar. The opposite responses were noted in the salt stressed treatments of the Galapagos ecotype. Tissue sugar levels did not appear to be similarly correlated with salt stress in either species. Potassium content fell sharply during salinization in the Galapagos ecotype while in the esculentum cultivar it declined relatively little even at high levels of salinity. 相似文献
6.
The concentrations and composition of free amino acids in phloem sap from two cultivars of oats and barley, both susceptible to the aphid Rhopalosiphum padi, were determined by means of high performance liquid chromatography. Sap was collected from excised aphid stylets at three developmental stages (seedlings, tillering plants and plants undergoing stem elongation) from plants given or not given fertiliser and grown outdoors. In connection, the growth of individual R. padi nymphs was estimated at the same phenological stages on plants grown in the greenhouse. The content of free amino acids was consistently higher in seedlings than in plants at the early tillering stage. Only in seedlings did the addition of fertiliser increase amino acid levels. Barley phloem sap contained more free amino acids than that of oats when fertiliser was added and at later developmental stages. Phloem sap of oats and barley showed similar patterns in their composition of free amino acids at the seedling stage, but as the plants grew older the patterns became increasingly different. Plants given fertiliser had higher amounts of dicarboxylic amino acids (glutamic and aspartic acid) than unfertilised plants. The concentrations of γ-amino butyric acid, glycine, histidine, and methionine were very low in all treatments. The relative growth rates of R. padi nymphs were low when amino acid content was low and vice versa. The results are discussed in relation to host plant suitability and plant resistance mechanisms. 相似文献
7.
Nicotiana rustica plants were grown under two grades of salinity conditions, 50 m M and 100 m M NaCl. High levels of NaCl added to the nutritive solution produced a progressive absorption of Na in detriment of K, thereby causing an ionic disequilibrium, particularly in leaves. The weight and longitudinal growth of plants grown under salinity conditions were lower than control plants, while their protein content was higher. Deficit of K induced by salinity increased the levels of free amino acids, especially of aspartic acid, glutamic acid and proline. On the other hand, the levels of nicotine in leaves of treated plants were lower than controls. In contrast, treatment of the plants with 100 m M NaCl induced in general an increase of nicotine in the roots. These results indicate that there was an unclear effect of salinity either on synthesis or on translocation of nicotine from roots to leves. 相似文献
8.
The effect of the nitrogen source (ammonium and nitrate) and its interaction with magnesium on various physiological processes
was studied in sunflower plants ( Helianthus annuusL.). Plants were grown in hydroponic culture with nitrate (5 mM) or ammonium (5 mM) and four concentrations of magnesium (0.1, 0.8, 5 and 10 mM). After 2 weeks, growth, gas exchange and fluorescence parameters, soluble carbohydrates, free amino acids, soluble protein
and mineral elements were determined. Ammonium nutrition resulted in a reduction of dry matter accumulation, as well as in
a decrease in the CO 2 assimilation. Moreover, ammonium-fed plants showed a greater content of free amino acids, soluble protein, Rubisco and anions,
and a lower cation content, mostly Mg 2+. The presence of high levels of Mg 2+ in the nutrient solution containing NH 4
+ resulted in a stimulation of growth and CO 2 assimilation to the levels observed in nitrate-fed plants. The lower photosynthetic rate of ammonium-fed plants grown with
low level of magnesium does not seem to be due to a lower photosynthetic pigment content, or a deficiency in Photosystem II
activity, or to lower Rubisco content. Hence, Rubisco activity or other enzymes involved in CO 2 fixation could have been affected in ammonium-fed plants.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
9.
Certain soybean [ Glycine max (L.) Merr.] cultivars that are grown in saline nutrient cultures are killed when the inorganic phosphate (Pi) concentration in the substrate exceeds 0.10 m M. To determine the role of Na and Cl on this adverse salinity×Pi interaction, four cultivars, Clark, Clark 63, Lee, and Lee 74 were grown in the greenhouse in nutrient solutions salinized with 1) Cl and NO 3 salts to produce treatments with variable amounts of Cl or 2) with NaCl or KCl and CaCl 2 to obtain treatments with and without Na. At an osmotic potential of ?0.34 MPa, all salts enhanced Pi uptake and accumulation in the tissue of plants grown in ≧0.12 m M substrate Pi. Leaf Cl concentration was linearly related (r 2≥0.9) to the mole fraction (mf) of Cl in the substrate, therefore excess substrate NO 3 did not greatly influence leaf Cl accumulation. Foliar injury was only observed on plants grown in saline solutions at high Pi (≥0.12 m M) and was not alleviated when KCl replaced NaCl in the substrate. This indicates that Na did not play a direct role in the salinity×Pi interaction. However, as the mf of Cl increased, severity of injury increased. The severity of injury, and its symptoms, were dependent upon leaf P and Cl concentration. Plants died when Cl and P in their leaves exceeded 800 and 600 mmol kg ?1 dry wt, respectively ( e.g., Clark 63 grown at mf of Cl=1). The necrotic leaves were beige in color. Leaves that contained P in excess of 600 mmol kg ?1 dry wt and Cl between 150–200 mmol kg ?1 dry wt, were severely injured and reddish-brown in color ( e.g., Clark 63 at mf of Cl=1/4 and Lee 74 Pi grown at mf of Cl=1). When leaf Cl was below 150 mmol kg ?1 dry wt, development of reddish-brown coloration in the leaves was sporadic. The adverse salinity×Pi interaction observed on these soybean variaties, therefore, was caused by a synergistic interaction between P and Cl in the leaves. 相似文献
10.
A method is described by which the effect of chelated Fe can be compared with the effect of ionic Fe in nutrient solution cultures over a prolonged period of time. Plants are grown in two solutions in succession: the one containing all nutrient elements except Fe, the other one containing the Fe compound together with Ca(NO 3) 2. In experiments with soybeans ( Glycine maxima ( L.) Merr.) and with corn ( Zea mays L.) it was shown that a 7-day cycle with the ratio of 4 days nutrient solution to 3 days Fe solution resulted in growth and Fe nutrition similar to plants grown with a normal nutrient solution. 相似文献
11.
Young plants of a rhizomatous grass Calamagrostis epigejos (L.) Roth were grown from seed in nutrient solutions containing nitrogen in concentrations 0.1, 1.0, and 10 mM. After six
weeks of cultivation the plants were defoliated and changes in growth parameters and in content of storage compounds were
measured in the course of regrowth under highly reduced nitrogen availability. Plants grown at higher nitrogen supply before
defoliation had higher amount of all types of nitrogen storage compounds (nitrates, free amino acids, soluble proteins), which
was beneficial for their regrowth rate, in spite of lower content of storage saccharides. Amino acids and soluble proteins
from roots and stubble bases were the most important sources of storage compounds for regrowth of the shoot. Faster growth
of plants with higher N content was mediated by greater leaf area expansion and greater number of leaves. In plants with lower
contents of N compounds number of green leaves decreased after defoliation significantly and senescing leaves presumably served
as N source for other growing organs. Results suggest that internal N reserves can support regrowth of plants after defoliation
even under fluctuating external N availability. Faster regrowth of C. epigejos with more reserves was mediated mainly by changes in plant morphogenesis. 相似文献
12.
The free amino acid and ammonia pools of Azolla caroliniana were analyzed by quantitative column chromatography on columns capable of separating all of the nitrogenous constituents normally found in physiological fluids. Comparisons were made of plants containing symbiotic algae and grown on nitrogen-free media, plants grown on media containing nitrate, and algae-free plants also grown on nitrate media. The major feature of the data was a very high level of intracellular ammonia found in plants which contain N 2-fixing algal symbionts. In addition to the more usual amino acids, serine and cystathionine were found in the free amino acid pool. 相似文献
13.
During high altitude acclimatization the highest number of amino acids were found in temperate Artemisia species ( A. vestita) and the lowest one in tropical species ( A. scoparia). The amount of free amino acids in temperate Artemisia species was higher when this was grown at 3600 m altitude. In A. scoparia, A. vulnaris and A. parviflora, the higher amount of individual amino acids was ascertained in plants grown at lower altitudes.
In A. vestita, the nitrogen contents per unit dry matter was lower in plants grown at high altitude. In the other three species, the contents
were significantly higher in the leaves of plants grown at a higher altitude. The nitrogen contents per unit leaf dry matter
determined during active growth of plants were minimum in A. parviflora and maximum in A. vestita. With progressing plant senescence the concentration of nitrogen decreased in all species studied. 相似文献
14.
Two sugar beet ( Beta vulgaris L.) genotypes were cultivated at different K +/Na + concentration in nutrient solutions (mM, 3/0 (control groups), 0.03/2.97 (K-Na replacement groups), and 0.03/0 (K deficiency groups)) to investigate the effects of potassium deficiency and replacement of potassium by sodium on plant growth and to explore how sodium can compensate for a lack of potassium. After 22 days of growth were determined: (i) dry weights of leaves, stems, and roots, (ii) the Na + and K + contents, (iii) MDA level, (iv) the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX), and (v) the level of free amino acids. Potassium deficit inhibited plant growth, decreased the K + content in leaves and roots, activated GPX and SOD, suppressed CAT activity, and increased the content of most amino acids. In K-Na replacement groups, the effects of K + deficiency, including changes in the MDA level, antioxidant enzyme activities, and the level of free amino acids, were alleviated, but the degree of recovery did not reach the values characteristic for the control groups. Based on these results, we concluded that low potassium could lead to the inhibition of seedling growth, oxidative damage, and amino acid accumulation. While sodium was able to substitute potassium to a large extent, it cannot fulfil potassium fundamental role as an essential nutrient in sugar beet. 相似文献
15.
Summary Loss of amides and amino acids from pine roots was two and a half times greater from roots grown in phosphate-deficient nutrient solution than from those grown in complete nutrient solution and ten times greater than from plants grown in nitrogen-deficient solution. The major factor in increased loss from phosphate-deficient plants was an increase in free amides and amino acids in these plants. Increased amide and amino acid loss from phosphate-deficient plants may have important consequences in micro-organism—plant interactions. 相似文献
16.
Soybeans ( Glycine max [L.] Merr.) grown in Ni-deficient nutrient solutions accumulated toxic urea concentrations which resulted in necrosis of their leaflet tips, a characteristic of Ni deficiency. Estimates of the Ni requirement of a plant were made by using seeds produced with different initial Ni contents. When compared to soybeans grown from seeds containing 2.5 nanograms Ni, plants grown from seeds containing 13 nanograms Ni had a significantly reduced incidence of leaflet tip necrosis. Plants grown from seeds containing 160 nanograms Ni produced leaves with almost no leaflet tip necrosis symptoms. Neither Al, Cd, Sn, nor V were able to substitute for Ni. In other experiments, a small excess of EDTA was included in the nutrient solution in addition to that needed to chelate micronutrient metals. Under these conditions, nodulated nitrogen-fixing soybeans had a high incidence of leaflet tip necrosis, even when 1 micromolar NiEDTA was supplied. However, in nutrient solutions containing inorganic sources of N, 1 micromolar NiEDTA almost completely prevented leaflet tip necrosis, although no significant increase in leaf urease activity was observed. Cowpeas (Vigna unguiculata [L.] Walp) grown in Ni-deficient nutrient solutions containing NO3 and NH4 also developed leaflet tip necrosis, which was analogous to that produced in soybeans, and 1 micromolar NiEDTA additions prevented these symptoms. These findings further support our contention that Ni is an essential element for higher plants. 相似文献
17.
Percentage germination, and growth of hyphae from single conidia of Erysiphe cichoracearum DC., were measured on leaf discs from topped and intact tobacco plants, grown in aerated nutrient solutions consisting of basal medium plus large or small amounts of potassium. The effect of supplying sodium was also studied. Discs were incubated on water and on 10% sucrose solution. Changes in free amino nitrogen and carbohydrate in comparable uninfected leaf discs, before and after incubation, were also measured. Potassium deficiency resulted in more free amino nitrogen and soluble carbohydrate and less insoluble carbohydrate, per cm. 2 of leaf. Spore germination was not greatly affected by treatments, though it was usually less on discs from potassium-deficient leaves. The pathogen grew slower on potassium-deficient leaf discs, whether they were incubated on water or on sucrose. Incubating discs from some leaves on sucrose, compared with water, gave greatly increased sugar content and less fungal growth; discs from other leaves had a much smaller increase in sugar, and hyphal length was similar to that on discs incubated on water. Sodium, when potassium was scarce, increased potassium deficiency symptoms, free amino nitrogen and sugar content, and resistance to powdery mildew. 相似文献
18.
Free amino acid constituents were determined of grape stem and Phylloxera leaf gall callus in tissue culture. Fast, medium and slow growing single cell clones of, respectively, stem and gall origins were grown on a mineral salt-sucrose medium supplemented with coconut milk and α-naphthaleneacetic acid. Stem and gall clones showed qualitative similarities and quantitative variations in the amino acids and nitrogenous constituents. Nineteen amino acids, glucosamine, ethanolamine, sarcosine, methionine sulfoxides and ammonia were identified. Two free polypeptides accounted for over 30% of the amino compounds in the stem and gall callus tissues which were not found in the intact plant parts. Stem clones of different growth rates grown on agar showed generally an excess of amino acid constituents over gall tissues of similar growth rates, except for the free polypeptides. Fast growing stem clones grown on agar medium contained lower amounts of certain amino acids than the fast growing gall clones, but when grown in liquid medium they contained higher amounts of these acids than the gall clones. The total and nonsoluble nitrogen of stem clones were higher than in the gall clones. Tissue cultures differed from the original plant parts with respect to their free polypeptides and high amino acid contents. 相似文献
19.
Summary The effects of P nutrition under various salinity levels on the protein, amino acids, and nutrients in mature wheat grain were studied. Mexican dwarf wheat ( Triticum aestivum L. var Inia) was grown to maturity in solution cultures with variable concentrations of P (0.5-, 5-, 25- and 50 mg P/l) in combination with NaCl at concentrations producing osmotic potentials ( s) of –0.4-, –1.4-, –2.4- and –4.4-bars. All other essential nutrients were present in adequate concentrations for vigorous plant growth.Increasing levels of P in the nutrient solutions tended to decrease the grain yield, N, Cl, protein-glutamic acid,-proline,-leucine,-glycine, and-serine, while P, K, Mg, Zn, Mn and Cu in the grain were increased. The sum of all protein amino acids in the grain decreased as the concentration of P increased in the nutrient solution. The effect of P on the individual and sum of amino acids tended to show peak amounts at the 5.0 mg P/l treatment level.Increased levels of salinity significantly reduced grain yield, N, proteinglutamic acid,-proline,-leucine,-glycine,-serine,-aspartic acid,-alanine, and-phenylalanine in the grain. The sum of the protein amino acids (mol/g dry wt.) was decreased in the grain from plants grown at –4.4 bars salinity level, but not in the grain from plants receiving less saline treatments. The concentrations of free amino acids: serine, aspartic acid, glutamic acid, alanine, and arginine were lower in the grain produced at the –4.4 bars salinity than at –0.4 bars salinity level. The sum of free amino acids (mol/g dry wt.) in the grain were decreased at the highest salinity level as compared with concentrations found for grain produced at lower salinity levels.There were some interactions found between P and salinity on the protein amino acids and nutrients in the grains.The amounts of essential amino acids expressed in mg/g of protein were not significantly affected by the increasing levels of P and salinity in the nutrient solution and they were found in adequate or greater amounts than those recommended by the World Health Organization and FAO. 相似文献
20.
Lettuce ( Lactuca sativa L.) and tomato ( Lycopersicon esculentum Mill.) plants were grown in purified nutrient solutions with and without the addition of 50 nanograms per milliliter V. These experiments showed that lettuce and tomato plants can be grown to maturity on nutrient solutions containing less than 0.04 nanogram per milliliter V with tissue concentrations of less than 2 to 18 nanograms per gram V. Growth and dry matter yield were comparable to those of plants grown on nutrient solutions containing 50 nanograms per milliliter with tissue levels of V from 117 to 418 nanograms per gram. Thus if V is an essential element for lettuce and tomato plants, the adequate tissue level would be less than 2 nanograms per gram V derivable from a growth medium containing less than 0.04 nanogram per milliliter V. 相似文献
|