Reproductive function in the Australian Swamp buffalo bull: Age effects and seasonal effects

Swamp buffalo exhibited seasonal variations in daily sperm production and in daily sperm production per gram of testis parenchyma. Maximum rates occurred in the late wet season and early dry season. There was no spermatogenesis detected in 1-yr-old bulls. Daily sperm production per gram of testis parenchyma increased thereafter up to 3.5 yr of age, and was similar in all older age groups. Scrotal circumference in bulls of this age ranged from 20 to 21 cm. Scrotal circumference and daily sperm production increased rapidly up to maturity, and increased less rapidly thereafter. Mean+/-SEM daily sperm production per gram in 146 mature buffalo bulls was 14.04 x 10(6)+/-0.39 x 10(6) and mean+/-SEM daily sperm production was 1.86 x 10(9)+/- 0.07 x 10(9). Mean+/-SEM epididymal sperm reserves in adult bulls numbered 9.7 x 10(9) +/- 0.07 x 10(9). These were distributed between the caput, corpus and cauda epididymis in the proportions of 28.82, 14.63 and 60.55, respectively. Mean+/-SEM epididymal transit time was 5.65+/-0.24 d.     

Sexual development in early- and late-maturing Bos indicus and Bos indicus x Bos taurus crossbred bulls in Brazil

Two experiments were conducted to evaluate sexual development in early- and late-maturing Nelore (Bos indicus) and Canchim (3/8 Bos indicus x 5/8 Bos taurus crossbred) bulls and to determine predictors of sexual precocity, and pubertal and maturity status. In Experiment 1, 12 Nelore bulls where examined from 300 to 900 days of age. Puberty was characterized by an ejaculate containing > or =50 million sperm with > or =10% motile sperm, and maturity by an ejaculate containing > or =70% morphologically normal sperm. In Experiment 2, 28 Canchim bulls where examined from 295 to 488 days of age and puberty was characterized by an ejaculate containing > or =30% motile sperm. In both experiments, bulls were classified as early- or late-maturing based on age at puberty. Early-maturing bulls were younger (P < 0.05) than late-maturing bulls at puberty (527 days versus 673 days in Experiment 1 and 360 days versus 461 days in Experiment 2) and at maturity (660 days versus 768 days in Experiment 1). In general, early-maturing bulls were heavier and had greater scrotal circumference (SC), testes, and testicular vascular cone diameter than late-maturing bulls during the experimental period. Scrotal circumference adjusted for 365 days of age was a good predictor of sexual precocity; minimum yearling SC of 19 and 24 cm for Nelore and Canchim bulls, respectively, had the best predictive values. Early-maturing bulls were lighter and had smaller SC at puberty than late-maturing bulls; therefore, sexual precocity was not related to the attainment of a threshold body weight or testicular size earlier, but to lower thresholds in early-maturing bulls. When predictors of pubertal status were evaluated, SC had the best sensitivity/specificity relationship in Nelore bulls, and high sensitivity and specificity in Canchim bulls. When predictors of sexual maturity were evaluated in Nelore bulls, age, weight, and SC had similar sensitivity, specificity, and predictive values. At puberty, approximately 60% of the sperm present in the ejaculate were morphologically defective. Changes in semen quality after puberty in Nelore bulls were characterized by increased motility and proportion of morphologically normal sperm, with a decrease in the proportion of major sperm defects. In conclusion, early-maturing bulls were more developed in the pre-pubertal period and attained puberty at earlier stages of body and testicular development than late-maturing bulls. Yearling SC could be used to select bulls for sexual precocity and SC was the best predictor of pubertal status. Age, weight, and SC were equally good predictors of sexual maturity in B. indicus bulls.     

Influence of season of birth on growth and reproductive development of Brahman bulls

Seasonal effects on reproduction are more dramatic in Bos indicus than Bos taurus cattle. This experiment evaluated reproductive development of fall- (n=7) versus spring- (n = 10) born Brahman bulls to determine if season of birth affects reproductive development. Measurements of growth and reproductive development began after weaning and continued at bi-weekly intervals until each bull reached sexual maturity. Different stages of sexual development were classified according to characteristics of the ejaculate and included first sperm in the ejaculate, puberty (> 50 x 10(6) sperm/ejaculate), and sexual maturity (two ejaculates with > 500 = 10(6) sperm/ejaculate). Average daily increases in all measured traits were similar in fall- and spring-born bulls and there were no differences in age, body weight, scrotal circumference, or paired testis volume between groups at first sperm or puberty. However, fall-born bulls were older (P < 0.05) than spring-born bulls at sexual maturity (553 days versus 481 days, respectively) as the interval between puberty and sexual maturity was longer (P < 0.05) in fall- than in spring-born bulls (82 days versus 54 days, respectively). The prolonged interval between puberty and sexual maturity in fall-born calves coincided with a short photoperiod (winter) whereas the short interval between puberty and sexual maturity in spring-born calves coincided with a long photoperiod (summer). In conclusion, season of birth affected sexual development; photoperiod might be involved in regulating testicular function immediately after puberty in Brahman bulls.     

Effect of growth rate from 6 to 16 months of age on sexual development and reproductive function in beef bulls

Sexual development and reproductive function were studied in 22 Angus × Charolais and 17 Angus bulls from 6 to 16 mo of age. Associations of average daily gain (ADG) and body weight with ages at puberty and at maturity (satisfactory semen quality), scrotal circumference, paired-testes volume and weight, testicular vascular cone diameter and fat thickness, scrotal temperature, sperm production and morphology, and testicular histology, were determined. There were no significant correlations between cumulative average daily gain and any of the end points investigated. Body weight at various ages was negatively correlated with ages at puberty and maturity in Angus × Charolais bulls, positively correlated with paired-testes weight in Angus × Charolais and Angus bulls, and positively correlated with seminiferous tubule volume in Angus bulls (P < 0.05). Semen quality improved gradually with age and the interval between puberty and maturity (mean ± SD; 309.4 ± 29.7 and 357 ± 42 days of age) was approximately 50 days. Age, weight, scrotal circumference, and paired-testes volume were all good predictors of pubertal and mature status, with moderate to high sensitivity and specificity (71.6% to 92.4%). In summary, growth rate between 6 and 16 mo of age did not affect sexual development and reproductive function in beef bulls. However, greater body weight at various ages was associated with reduced age at puberty and maturity, and with larger testes at 16 mo of age, indicating that improved nutrition might be beneficial, but only when offered before 6 mo of age. Average daily gains of approximately 1 to 1.6 kg/day did not result in excessive fat accumulation in the scrotum, increased scrotal temperature, or reduction in sperm production and semen quality, and could be considered “safe” targets for growing beef bulls.     

Slow increase of Sertoli cell efficiency and daily sperm production causes delayed establishment of full sexual maturity in the rodent Chinchilla lanigera

Puberty in the male Andean rodent Chinchilla lanigera occurred approximately 3 mo after birth, whereas full sexual maturity was established much later. The objective of the present study was to investigate testis function in postpubertal chinchillas, with an emphasis on the estimation of seminiferous epithelium cycle length (n=6) and Sertoli cell (SC) and spermatogenic efficiencies (n=26). Samples of testes were collected between May and November. Each spermatogenic cycle lasted 10.2d and the total duration of spermatogenesis was approximately 46 d. The SC efficiency (spermatids/SC) and the daily sperm production per testis gram increased markedly (P<0.05) from 5 to 17-22 mo of age, whereas the conversion rates of type A1 spermatogonia to preleptone and the number of spermatids per pachytene remained stable (P>0.05) from 5 to 30 mo. Therefore, efficiency of the spermatogenic process increased equally during all phases of spermatogenesis. In conclusion, based on the gradual and striking postpubertal increases for SC and sperm production, we inferred that more undifferentiated spermatogonia and/or spermatogonial stem cells were produced and therefore, that the chinchilla might represent a good experimental model to investigate regulation of this crucial aspect of spermatogenesis.     

Testicular and epididymal function during the peripuberal period in Brahman bulls receiving various amounts of protein degradable in the rumen

Thirty-nine Brahman bulls with an initial age and weight of 301.7 +/- 4.1 d and 202.7 +/- 4.7 kg, respectively, were randomly allocated to 1 of 2 dietary treatment groups within age, weight and sire in order to study the influence of source of protein and stage of peripuberal period on testicular and epididymal function. In the soybean meal treatment the amount of protein undegradable in the rumen averaged 47%, while it was 72% in the fish meal treatment. The supplements were isocaloric and isonitrogenous. Bulls were electroejaculated, and castrations were performed randomly in a predetermined order when the first ejaculate with the first motile sperm cells (Stage 1), 10 to 25 million (Stage 2), and 50 million or more sperm cells (Stage 3 - puberty) was obtained. Testicular and epididymal traits were analyzed for a single testicle and epididymis. Daily sperm production, daily sperm production per gram of testicular parenchyma, testicular weight and testicular parenchyma weight were not affected by treatment. Bulls receiving fish meal had heavier (P < 0.01) epididymis than soybean meal-fed bulls (6.6 +/- 1.0 vs 3.9 +/- 0.6 g) but similar (P > 0.05) epididymal sperm reserves. Daily sperm production (1 testicle) was 115.2 +/- 0.1, 447.4 +/- 0.1, 792.7 +/- 0.1 million sperm cells, and daily sperm production per gram of testicular parenchyma was 1.5 +/- 0.5, 3.2 +/- 0.6 and 6.4 +/- 0.6 million sperm cells for bulls at Stage 1, 2 and 3, respectively. Sire and amount of undegradable intake protein had significant (P < 0.05) affects on the distribution of epididymal sperm reserves, with soybean meal-fed bulls having the higher proportions of epididymal sperm reserves in the cauda epididymis.     

Patterns of puberal development in Sahiwal and Brahman cross bulls in tropical Australia. I. Growth and semen characteristics

Reproductive development was monitored in 13 1 2 Brahman (BX), 20 3 4 BX, 8 1 2 Sahiwal (SX) and 11 3 4 SX crossbred bulls between one and two years of age. Liveweight increased during wet season (0.7 kg/day) and decreased during dry season conditions (-0.25 kg/day). Scrotal circumference (SC) increased from 18.6 to 25.0 cm, but decreased slightly during the later stages of the dry season. First ejaculates containing 50 x 10(6) spermatozoa were obtained from 25% of the bulls at 15.3 months of age and from 84% by two years of age. Sperm motility and morphology showed no consistent pattern, but number of sperm per ejaculate increased and seminal fructose concentrations decreased with advancing age. Significant genotype differences were noted for SC and seminal characteristics. Estimated age and liveweight at puberty were 570, 563, 578 and 542 days, and 251, 265, 247 and 256 kg for 1 2 BX, 3 4 BX, 1 2 SX and 3 4 SX bulls, respectively.     

Influence of unilateral castration and increased plane of nutrition on sexual development of Holstein bulls. I. Growth and sperm production

Sixty-five Holstein bull calves were used to study the effects of unilateral castration (UC) and increased plane of nutrition on the growth and development of the reproductive system. Bulls were slaughtered at 1 wk., 2, 4, 8 and 16 months. Half of each slaughter group above one week was unilaterally castrated at 7 days of age. Half of the bulls remaining at 6 months of age received 90% of their recommended daily TDN allowance while the remainder received 120%. Compensatory hypertrophy was evident as early as 2 months and the degree of compensation increased for the duration of the experiment (Age x UC, P<.01). By 16 months of age the remaining testis of UC animals was 73% heavier than the average testis weight of intact bulls. While epididymal weight was significantly increased by UC, seminal vesicle weight was not. UC bulls produced significantly more sperm per testis than intact bulls both from the onset of puberty to slaughter and for the 16 week period prior to slaughter. Testis sperm concentration was similar in UC and intact bulls. UC at one weel of age caused greater testis growth and greater sperm production per testis, but did not promote earlier puberty.     

Nutritional influences on growth and onset of puberty in Boran and Boran x Friesian bulls in Ethiopia

Twenty-seven Boran and 37 Boran x Friesian crossbred bulls were weaned at 6 months of age and randomly assigned to either a dry season supplementary feed containing 16% crude protein or control groups. Data were collected on body weight, wither height and scrotal circumference (SC) at monthly intervals. Bulls were also challenged with 100 mug of gonadotropin releasing hormone (GnRH) at 2-month intervals and plasma testosterone responses were determined. Semen was collected at monthly intervals by electroejaculation. Post weaning average daily weight gain was significantly (P<0.001) higher in supplemented than in control groups (302 vs 208 g/day). Scrotal circumference growth rates were also significantly (P<0.001) higher in supplemented than in control bulls (0.38 vs 0.31 mm/day). Bulls fed supplemented diets were younger (430 vs 473 days; P<0.001) and heavier (217 vs 203 kg; P<0.05) and had better semen quality at puberty than the control bulls. There was a trend for larger SC at puberty in supplemented than in control bulls (P=0.06). There were no significant differences in wither height or in testosterone response to GnRH at puberty between supplemented and control bulls. Body and SC growth rates to puberty for Boran and Boran x Friesian bulls were 189 and 321 g/day (P<0.001) and 0.27 and 0.42 mm/day (P<0.01), respectively. Boran bulls were older (513 vs 406 days; P<0.01) and lighter (201 vs 215 kg; P<0.05), and had smaller SC (23.9 vs 26 cm; P<0.01) at puberty than Boran x Friesian bulls. Testosterone responses to GnRH increased (P<0.001) with age and were significantly higher (P<0.05) in supplemented and in Boran x Friesian bulls than in control and Boran bulls. Wither height, testosterone response to GnRH and semen quality at puberty did not differ between breeds. Dry season supplementation improved growth rate and enhanced sexual development and maturity in both Boran and Boran x Friesian bulls, and would be a useful management strategy for enhancing fertility in bulls in the tropics.     

Effects of dietary gossypol on aspects of semen quality, sperm morphology and sperm production in young Brahman bulls

Eight young reproductively normal Brahman bulls (average age and bodyweight 20 months and 500 kg, respectively) received either cottonseed meal delivering 8.2 g free gossypol/bull/d (treatment group, n=4) or soybean meal (control group, n=4) for 12 wk. After adjustment (1 wk), weekly procedures (11 wk) included blood collection, scrotal circumference measurement and electroejaculation. Semen assessments included sperm motility, percentage of live spermatozoa, general sperm morphology (using brightfield microscopy), and midpiece morphology (using DIC microscopy). After sacrifice (Week 12), sperm production rates (daily and per gram testicular parenchyma) were determined. Treated bulls did not differ from controls in scrotal circumference or the percentage of live spermatozoa. Sperm motility differed at Weeks 9 (P<0.05), 10 and 11 (both P=0.06). Treated bulls had fewer normal spermatozoa at Weeks 5 (P<0.05), 6 (P<0.01) and 7 thru 11 (P<0.001). Beginning from Week 3, treated bulls showed an increased proportion of sperm midpiece abnormalities (P<0.05) which stabilized at 52 to 62.5% between Weeks 5 and 11 (P<0.01 or P<0.001). Treated bulls also had lower sperm production than untreated bulls, both on a daily (P<0.01) and per gram testicular parenchyma (P<0.001) basis. A cottonseed supplement providing 8.2 g of free gossypol per bull per day had adverse effects upon both sperm morphology and spermatogenesis in young Brahman bulls, with the former being first evident within 3 to 4 weeks of feeding of cottonseed meal.     

Determination of onset of puberty in Zebu bulls under tropical conditions of northern Nigeria

Age at puberty and related phenomena were investigated in White Fulani (Bunaji - WF), Sokoto Gudali (SG) and Friesian X Bunaji (FRXWF) Zebu bulls from 28 weeks to 72 weeks of age. Pubertal traits studied included body weight, heart girth, scrotal circumference and sperm production. Puberty was defined as the age at which an ejaculated semen contained at least 50 x 10(6) spermatozoa with a minimum of 10% motility. Age at puberty was 66.89 +/- 1.0, 73.4 +/- 2.2 and 62.4 +/- 10.7 weeks for WF, SG and FRXWF bulls respectively. The Bunaji bulls were significantly younger than the Sokoto Gudali bulls at production of first motile sperm cell and also at puberty (P<0.01). The SG bulls were significantly heavier (P<0.01) and had larger heart girth (P<0.05) than the FRxWF at production of first motile sperm cells. Body weight and heart girth for all the breeds were not different at puberty (P>0.1). Scrotal circumference of the bulls between the breeds at production of first motile sperm cells and at puberty, did not differ (P 0.>1), despite the variations in age, weight and heart girth at production of first motile sperm cell and at puberty. It is concluded from the data obtained that the indigenous breeds of bulls (Bos indicus ) seem to attain puberty later than exotic breeds (Bos taurus ). The inter-play of genetic, nutritional and environmental factors as a contribution to this delay in attainment of puberty could not be ruled out.     

Peripubertal traits of Brahman bulls in Yucatan

In order to investigate elements of puberty in Brahman (Bos indicus) bulls, 12 animals were studied from 10 mo of age until puberty, which was defined as the moment of production of the first ejaculate with at least 50 x 10(6) sperm with 10% progressive motility. Body weight and scrotal circumference were recorded every 2 weeks. From 14 mo of age onwards, the bulls were electroejaculated at the same intervals and ejaculates were evaluated. Blood samples were taken from 6 of the bulls every 30 min for 6 h at 12, 14, 17 and 20 mo of age to study serum concentrations of testosterone, which were determined by RIA. Mean age (+/- SEM) at puberty was 17 +/- 0.4 mo, body weight was 374.6 +/- 22.5 kg and scrotal circumference was 28.6 +/- 0.6 cm. Serum testosterone increased from 12 to 17 mo and then decreased slightly, mean (+/- SEM) concentration being 0.4 +/- 0.1, 1.4 +/- 0.4, 2.9 +/- 0.5 and 2.7 +/- 0.3 at 12, 14, 17 and 20 mo of age, respectively. It was concluded that Brahman bulls, under the conditions studied, attain puberty between 16 and 17 mo of age, with considerable variation, and that serum concentrations of testosterone prior to puberty seem to form a pattern similar to that shown by Bos taurus bulls, however the pattern occurs at a later age.     

Testicular development and its relationship to semen production in Murrah buffalo bulls

The objectives of this study were to determine the relationship of age and body weight to testicular development and to establish norms for breeding soundness evaluations of Murrah buffalo bulls. Testicular measurements of 133 Murrah buffalo bulls of various ages were recorded with a caliper and a tape. Semen was collected twice a week for 5 weeks from groups of bulls which were 25-36 (n=17), 37-48 (n=16), 49-60 (n=14), of >60 (n=10) months of age. After examining volume, sperm concentration, and progressive motility semen was diluted in Tris-citric acid-egg yolk-fructose extender and frozen in 0.5 ml French straws. Testicular measurements of buffalo bulls were lower than those recorded for European breeds of cattle bulls. Nevertheless, like cattle bulls, scrotal circumference was highly correlated with other testicular measurements. Also, it had a significant positive relationship with semen volume and sperm concentration per ejaculate. Average sperm output per week in order of increasing age group was 15.3, 18.2, 19.8 and 23.6 x 10(9). Corresponding values for sperm output per week per gram of testis were 59.1, 45.8, 41.1, 36.2 x 10(6) indicating a reduction in spermatogenesis per unit of testis with advancing age. Compared to European breeds, daily sperm output in Murrah bulls was nearly 45% lower, presumably due to their nearly 40% lower scrotal circumference than Holstein bulls of the same age. These results indicate that in buffalo, as in cattle, scrotal circumference is a useful indicator of potential sperm output and may serve as an important criterion for selecting young bulls as AI sires.     

Androgen binding protein, sperm production, semen output and LH and testosterone profiles in young postpubertal bulls

An androgen binding protein (ABP), which binds 5alpha-dihydrotestosterone with high affinity (Ka = 0.3 x 10(9) M(-1)), has been demonstrated in testicular and epididymal cytosols of 5 young post pubertal bulls (15-17 months old) of the Montbeliarde dairy breed. Simultaneously, daily sperm production (DSP), semen output and plasma LH and testosterone concentrations (from frequent samplings) were determined. ABP levels were 21 fmoles/mg protein in testis and 59, 22 and 43 fmoles/mg, respectively, in caput, corpus and cauda epididymis. Mean DSP, per gram of testis, was 16.6 x 10(6) spermatozoa, and the mean sperm output was approximately 1.5 x 10(9) spermatozoa per ejaculate. Mean LH and testosterone levels were 1.5 ng/ml and 2.1 ng/ml, respectively. One bull (882) was clearly distinguishable from the others, in showing higher ABP and testosterone levels together with a lower daily sperm production. Results of this study may (1) suggest a physiological role of ABP in sperm epididymal maturation and (2) give a new parameter in the evaluation of individual bulls testicular function.     

Gonadal sperm reserve in purebred Landrace and Large White boars of high average daily gain

The present study investigated the effects of average growth rate (AGR) levels and age on the number of sperm cells per gram of testis parenchyma and on the gonadal reserve in Landrace (LD) and Large White (LW) boars. In Experiment 1, the effects of breed (LD, LW), level of AGR from birth up to 90 days of age (Level 1: 384 +/- 32 g/day; Level 2: 512 +/- 22 g/day; Level 3: 624 +/- 41 g/day), and age (13, 15, 17, 19 and 21 weeks) on testicular cell concentration were evaluated. Data were analyzed under a 2 x 3 x 4 factorial design. There were significant effects associated with breed (P < 0.001) and age (P < 0.001) but not with AGR (P > 0.05) on sperm cell number per gram of testicular parenchyma. The number of cells increased with age and was greater in LW than in LD young boars, mainly those up to 19 weeks of age. In Experiment 2, the effect of two AGR levels (Level 1: 649-694 g/day; Level 2: 813-885 g/day) from birth up to 100 kg body weight on the number of sperm cells per gram of testis parenchyma and on the gonadal reserve was investigated using 59 purebred LD and LW boars. The boars were castrated at 23, 25, 29 and 33 weeks of age. Age of boars significantly affected gonadal sperm reserve and the number of sperm cells per gram of testicular tissue (P < 0.001). Breed of boars and AGR Levels did not significantly affect number of sperm cells and gonadal sperm reserve (P > 0.05). It was concluded that the number of sperm cells in the testicular tissue of young boars is influenced by their breed and age, but not by the level of their AGR.     

Testicular development, histology, and hormone profiles in three yearling angus bulls with spermatogenic arrest

This article discusses the interactions between testis criteria and hormone profiles in Angus bulls with spermatogenic arrest. From 2 to 12 months (mo), testis diameter and hormone concentrations (basal and GnRH-stimulated) were evaluated in 27 bulls. At 12 mo, testes were excised. The z statistical test was used to determine whether parameters in three infertile bulls were different (P < 0.05) from those in 24 bulls with normal spermatouenesis. Bull 1 had Sertoli cell-only syndrome and Bull 2 had 90% of the tubules without germ cells and only A1 spermatogonia in the remaining. In Bull 3, germ cells did not advance beyond the primary spermatocyte stage. At 12 mo, testes of Bull 1 (99 g), Bull 2 (105 g) and Bull 3 (32 g) weighed less than those of normal bulls (251.5 +/- 56 g). Sertoli cell numbers/testis in Bull 1 (3.8 x 10(9)) and Bull 2 (4.3 x 10(9)) were not different from those in normal bulls (4.9 +/- 0.3 x 10(9)), but were reduced in Bull 3 (1.6 x 10(9)). The number of Leydig cells per gram of testis parenchyma was higher in Bull 1 (5.4 x 10(7)), Bull 2 (7.3 x 10(7)) and Bull 3 (19 x 10(7)) than in normal bulls (3.6 +/- 0.2 x 10(7)). In Bulls 1 and 2, basal and GnRH-stimulated LH, FSH, testosterone (T), androstenedione (delta4A) and estradiol 17-beta (E2) were within normal ranges at most ages. However, basal FSH and LH were greater in Bull 3 than in normal bulls, probably the causes for higher Leydig cell density. Also in the same animal, GnRH induced lower responses in LH and FSH, consequence of low basal T and E2 at some ages. Basal and GnRH-stimulated delta4A in Bull 3 were greater than in normal bulls after 6 mo, indicating impairment of Leydig cell differentiation. Deficiency in hormone secretion did not appear to be the cause of infertility, which points toward impaired gonadal responses or secretion of intratesticular factors, or genetic defects. Moreover, infertile animals may not always show pronounced changes in hormone secretion, but evaluation of testis growth around puberty can help identify those animals that do not have proper gonadal development.     

Testicular ultrasonogram pixel intensity during sexual development and its relationship with semen quality, sperm production, and quantitative testicular histology in beef bulls

This study was conducted to evaluate testicular ultrasonogram pixel intensity during sexual development in bulls and to determine its relationship with semen quality, sperm production, and quantitative testicular histology. Beef bulls (N = 152) were examined from 14 - 26 to 70 - 74 wk of age in four different years. Testicular echogenicity increased during sexual development, but the pattern of change differed among years. Echogenicity increased between 26 and 42 to 46 wk of age in 2 yr, but increased considerably earlier in the other 2 yr, reaching maximum values at 34 wk of age. Because increased echogenicity was likely associated with testicular changes leading to initiation of spermatogenesis, these differences were difficult to explain considering that age at puberty did not differ significantly among years. When data were evaluated according to age normalized to puberty, echogenicity started to increase 16 to 12 wk before puberty and reached maximum values 4 wk before or at puberty. These results indicate that a certain developmental stage of the testicular parenchyma must be reached before puberty and that the composition of the parenchyma remained consistent after puberty. Testicular echogenicity was associated with sperm production, seminiferous tubule and epithelium area, and sperm morphology, but the associations were not consistent. Testicular echogenicity was a good indicator of pubertal and mature status, but was not superior to scrotal circumference. In conclusion, although testicular ultrasonogram pixel intensity analysis might be useful for research purposes, clinical application of this technology in the present form for bull breeding soundness evaluation is not justifiable.     

Gonadal and extragonadal sperm reserves in Boran and Boran x Friesian bulls raised on two planes of nutrition in the Highlands of Ethiopia

Gonadal and extragonadal sperm reserves were determined in testicular and epididymal tissues obtained from Boran (n=10) and Boran x Friesian (n=12) bulls fed either a high or low plane nutrition diet for a 1-year period. The bulls were 32 months of age at castration. Mean (+/-SEM) daily body weight gains over a 1-year period were 776+/-34 and 264+/-34 g/day (P<0.001) for bulls on high and low nutrition, and their respective body weights at castration were 458+/-17 and 276+/-17 kg (P<0.01). Mean body weights did not differ between Boran and Boran x Friesian bulls. Mean scrotal circumference measurements were 32.1+/-0.6 and 28.8+/-0.6 cm for the high and low planes of nutrition (P<0.01) and 31.8+/-0.7 and 29.2+/-0.6 cm for the Boran and the Boran x Friesian bulls (P<0.05). Paired testes and epididymal weights averaged 432+/-22 and 313+/-22 g (P<0.01) and 46.3+/-2.0 and 32.5+/-2.0 g (P<0.001) for bulls on the high and low planes of nutrition, respectively. Boran x Friesian bulls had significantly heavier (P<0.05) testes, but epididymal weithts did not differ between breeds. Although daily sperm production per gram was not influenced by either plane of nutrition or breed, daily sperm production differed between high (5.3 x 10(9)) and low nutrition (2.9 x 10(9)) bulls (P<0.001) and between Boran (4.6 x 10(9)) and Boran x Friesian (3.5 x 10(9)) bulls (P<0.01). Extragonadal sperm reserves were significantly higher in high than in low nutrition bulls (13.1 x 10(9) vs 6.9 x 10(9); P<0.001) and in Boran than Boran x Friesian bulls (12.9 x 10(9) vs 7.1 x 10(9); P<0.01). The cauda epididymis contributed 50 to 54% to the total epididymal sperm reserves. It was concluded that the plane of nutrition influenced growth rates and testes and epididymal weights, and it improved gonadal and extragonadal sperm reserves in young Boran and Boran x Friesian bulls.     

Testicular shape and its relationship to sperm production in mature Holstein bulls

This study was conducted to determine the relationship between testicular shape, scrotal circumference (SC) and sperm production. Twenty-seven mature Holstein bulls were evaluated subjectively and objectively for testicular shape as indicated by testicular length and width, then placed in 1 of 3 groups. Group 1 contained 17 bulls with a normal ovoid testicular shape and a length to width ratio of 1.61:1 +/- 0.01 (SEM). Group 2 was composed of 4 bulls with a long, slender testicular shape and a length to width ratio of 1.95:1 +/- 0.06 (SEM). Group 3 was comprised of 6 bulls with spheroid-shaped testicles and a length to width ratio of 1.3:1 +/- 0.03 (SEM). All the groups were statistically different for length to width ratios (P < 0.05). Length measurements from cranial to caudal pole of the testis proper were also different between groups (P < 0.05). Width or testicular diameter was different between Group 2 and Group 3 at P < 0.05; however, there was no difference between Group 1 and Group 2 or between Group 1 and Group 3. Predicted volumes and weights of testicles were not significantly different between groups. Scrotal circumference measurements were significantly different between groups (P < 0.05). Group 1 had an average SC of 43.07 +/- 0.36 cm (SEM), Group 2 of 39.33 +/- 1.18 cm (SEM) and Group 3 of 46.22 +/- 0.69 cm (SEM). Sperm production for a twice daily, 2-day-per-week collection schedule revealed a statistically significant difference for sperm output. A total of 2742 ejaculates was evaluated. A total of 1818 ejaculates was evaluated in Group 1, 440 ejaculates in Group 2 and 484 ejaculates in Group 3. The mean spermatozoal harvest per day for Group 1 bulls was 13.62 +/- 0.09 x 10(9) (SEM). Group 2 bulls with the longer-shaped testicles produced 14.82 +/- 0.18 x 10(9) (SEM) spermatozoa per day, and Group 3 bulls, with the more rounded testicle shape and the significantly larger SC produced 11.72 +/- 0.64 x 10(9)(SEM) sperm cells per day. All 3 groups were statistically different at the P = 0.05 level. The results suggest that prediction of sperm production may be dependent on factors other than SC, testicular volume, or weight. Testicular shape may influence sperm output in mature Holstein bulls.