地骨皮提取液对内皮祖细胞功能的影响

通过观察地骨皮提取液对高糖培养的人脐血内皮祖细胞(EPCs)的黏附、迁移、增殖等能力的影响,探讨地骨皮提取液对高糖所致的血管内皮损伤是否具有保护作用。分离人脐血单个核细胞,接种培养后收集贴壁细胞,采用双荧光染色法及ecNOS和Flk-1基因的表达对EPCs的生物学特征进行鉴定。将分离到的EPCs分成5组:正常对照NG组、高糖HG组、HG+地骨皮提取液不同浓度组(1 g/组、2 g/L组和4 g/L组);用重贴壁法测定EPCs黏附能力,改良Boyden小室法测定其迁移能力及CCK-8法测定增殖能力。结果显示:(1)HG组EPCs的黏附、迁移及增殖能力较NG组明显下降;(2)HG+地骨皮提取液不同浓度组EPCs黏附、迁移和增殖能力均比HG组高;对迁移和增殖能力的影响以2 g/L干预组效果最为明显。提示地骨皮提取液能部分恢复高糖对EPCs黏附、迁移及增殖能力的抑制,对高糖所致的血管内皮损伤具有保护作用。     

miR-92a对大鼠骨髓源性内皮祖细胞功能的影响

该文探讨了mi R-92a对大鼠骨髓源性内皮祖细胞(endothelial progenitor cells,EPCs)迁移、黏附、增殖以及血管形成能力等的影响。采用密度梯度离心法分离大鼠骨髓来源的单核细胞,体外培养于含10%胎牛血清的EGM-2MV完全培养基中。利用LipofectamineTM 3000分别将mi R-92a模拟物(mi R-92a mimics)、mi R-92a抑制剂(mi R-92a inhibitor)及其对应的对照组(negative control,NC)转染入第3~5代EPCs,即晚期EPCs。采用改良Boyden小室、黏附实验、CCK-8及Matrigel胶分别检测EPCs迁移、黏附、增殖及体外成血管能力。mi R-92a模拟物明显抑制了EPCs的迁移和体外成血管能力(P0.05),但不影响EPCs的黏附和增殖能力;而mi R-92a抑制剂则促进了EPCs的迁移和体外成血管能力(P0.05),亦不影响EPCs的黏附和增殖能力。结果表明,mi R-92a可抑制EPCs的迁移及体外成血管能力。该研究结果将为临床以mi R-92a和EPCs为靶点防治动脉粥样硬化性心血管疾病提供理论及实验依据。     

电磁辐射对大鼠内皮祖细胞和肾脏组织学的影响

目的:研究电磁辐射对体外培养骨髓来源的内皮祖细胞(EPCs)增殖、迁移、黏附能力的影响,并探讨其与肾脏疾病的可能关系。方法:密度梯度离心法获取大鼠骨髓单个核细胞(MNCs),接种至纤维连接素包被的培养板上,培养6d后进行免疫细胞化学和免疫荧光鉴定EPCs。采用MTT比色法、Transwell小室和黏附能力测定实验,观察平均功率密度为65mW/cm2,时间20min的电磁辐射对EPCs的增殖、迁移、黏附能力的影响;同等剂量全身照射大鼠,光镜和透射电镜观察大鼠肾脏组织学和超微结构的变化。结果:从大鼠骨髓能成功分离培养获得EPCs。EPCs的增殖、迁移、黏附能力较对照组显著下降;大鼠接受全身照射后各时相点无明显组织学改变,但超微结构显示在照射后3h后开始出现肾小球毛细血管袢足突肿胀,12h后出现部分足突融合。结论:电磁辐射导致EPCs生物功能显著减弱,肾小球超微结构改变,电磁辐射可能与起肾脏疾病的发生有关。     

电磁辐射对大鼠内皮祖细胞和肾脏组织学的影响

目的：研究电磁辐射对体外培养骨髓来源的内皮祖细胞（EPCs）增殖、迁移、黏附能力的影响,并探讨其与肾脏疾病的可能关系。方法：密度梯度离心法获取大鼠骨髓单个核细胞（MNCs）,接种至纤维连接素包被的培养板上,培养6d后进行免疫细胞化学和免疫荧光鉴定EPCs。采用MTT比色法、Transwell小室和黏附能力测定实验,观察平均功率密度为65mW/cm2,时间20min的电磁辐射对EPCs的增殖、迁移、黏附能力的影响;同等剂量全身照射大鼠,光镜和透射电镜观察大鼠肾脏组织学和超微结构的变化。结果：从大鼠骨髓能成功分离培养获得EPCs。EPCs的增殖、迁移、黏附能力较对照组显著下降;大鼠接受全身照射后各时相点无明显组织学改变,但超微结构显示在照射后3h后开始出现肾小球毛细血管袢足突肿胀,12h后出现部分足突融合。结论：电磁辐射导致EPCs生物功能显著减弱,肾小球超微结构改变,电磁辐射可能与起肾脏疾病的发生有关。     

细胞因子对内皮祖细胞功能影响的研究进展

内皮祖细胞(EPCs)是一种具有较强增殖能力的前体细胞,血管损伤或者缺血会刺激骨髓EPCs动员,迁移、归巢于相应的靶位,然后分化为内皮细胞(ECs),从而参与血管修复和血管新生。因此,EPCs的成功发现为缺血性和血管损伤性疾病的治疗提供了新策略。但是EPCs存在动员率低、靶向性较差和功能不全等问题。大量研究显示细胞因子对EPCs的动员、归巢、增殖和分化等均起着重要的调节作用,同时,通过调控细胞因子能改善EPCs的功能活性,因此选择合适的细胞因子来提高EPCs功能变得非常重要。现总结了近年来细胞因子对EPCs功能影响的研究进展,并提出有待解决的问题和作一定的展望。     

匹伐他汀对体外培养人脐静脉血内皮祖细胞数量及功能的影响

目的:通过体外培养人脐静脉血内皮祖细胞(endothelial progenitor cells,EPCs),观察他汀类新药(匹伐他汀)对EPCs数量及增殖、迁移和粘附功能的影响。方法:采用密度梯度离心法分离培养人脐静脉血单个核细胞,将其接种在包被有人纤维连接蛋白培养板上,培养7 d后,收集贴壁细胞,加入不同浓度匹伐他汀(分别为0.001μmol/L、0.01μmol/L、0.1μmol/L、1.0μmol/L)培养24h,用免疫荧光法观察EPCs吸收FITC-UEA-I和Dil-acLDL情况对EPCs进行鉴定,然后分别采用MTT比色法、改良的Boyden小室、粘附能力测定实验对各实验组测定,来观察匹伐他汀对EPCs数量及增殖、迁移和粘附功能影响。结果:匹伐他汀组与对照组相比,匹伐他汀显著提高了体外培养EPCs的数量及增殖、迁移与粘附能力(P0.05)。匹伐他汀浓度在0.1μmol/L时对EPCs影响达到最大。随着药物浓度的继续增大,EPCs的上述功能反呈下降趋势,但1.0μmol/L组仍高于对照组。结论:匹伐他汀能增加体外培养EPCs的数量及增殖、迁移和粘附能力,可作为EPCs培养的一种改良方法,为其更好的应用于临床具有重要的意义。     

内皮祖细胞移植在慢性阻塞性肺疾病小鼠体内分布

目的:观察经气管移植内皮祖细胞(EPCs)在烟雾暴露所致慢性阻塞性肺疾病模型小鼠中的分布及分化.方法:体外分离小鼠骨髓单个核细胞于EGM-2MV培养基中培养并鉴定.24只C57BL/6J小鼠随机分为正常对照组、COPD+PBS干预组及COPD+EPCs干预组;香烟烟雾暴露90 d建立慢性阻塞性肺疾病(COPD)小鼠模型;烟雾暴露结束后,COPD+PBS干预组及COPD+EPCs干预组分别经气管注入30 μ LPBS和30 μ L细胞悬液(含1×105个CM-DiI标记EPCs).移植后观察30 d处死小鼠,通过荧光显微镜观察移植细胞在肺内的分布及通过免疫荧光检测广谱细胞角蛋白的表达水平.结果:EPCs移植后30d可见EPCs分布于肺血管及气道,部分细胞表达上皮特异标志广谱细胞角蛋白.结论:EPCs移植后可定植COPD模型小鼠肺血管及气道,且可能转化为支气管及肺泡上皮细胞.     

匹伐他汀对体外培养人脐静脉血内皮祖细胞数量及功能的影响

目的：通过体外培养人脐静脉血内皮祖细胞（endothelial progenitor cells,EPCs）,观察他汀类新药（匹伐他汀）对EPCs数量及增殖、迁移和粘附功能的影响。方法：采用密度梯度离心法分离培养人脐静脉血单个核细胞,将其接种在包被有人纤维连接蛋白培养板上,培养7 d后,收集贴壁细胞,加入不同浓度匹伐他汀（分别为0.001 μmol/L、0.01 μmol/L、0.1 μmol/L、1.0 μmol/L）培养24 h,用免疫荧光法观察EPCs 吸收FITC-UEA-I 和Dil-acLDL情况对EPCs 进行鉴定,然后分别采用MTT 比色法、改良的Boyden小室、粘附能力测定实验对各实验组测定,来观察匹伐他汀对EPCs 数量及增殖、迁移和粘附功能影响。结果：匹伐他汀组与对照组相比,匹伐他汀显著提高了体外培养EPCs的数量及增殖、迁移与粘附能力（P〈0.05）。匹伐他汀浓度在0.1 μmol/L 时对EPCs影响达到最大。随着药物浓度的继续增大,EPCs的上述功能反呈下降趋势,但1.0 μmol/L 组仍高于对照组。结论：匹伐他汀能增加体外培养EPCs的数量及增殖、迁移和粘附能力,可作为EPCs 培养的一种改良方法,为其更好的应用于临床具有重要的意义。     

消渴丸对GK糖尿病大鼠内皮祖细胞功能的影响

目的:观察消渴丸对GK大鼠外周血中内皮祖细胞(endothelial progenitor cells,EPCs)数量及功能的影响。方法:消渴丸连续给药12周后测定并比较GK大鼠血清EPCs的粘附能力、迁移能力和体外生成血管能力。结果:消渴丸可使GK大鼠EPCs数量显著升高,其粘附能力、迁移能力和体外生成血管能力也明显改善。结论:消渴丸可以升高GK大鼠动脉血中EPCs数量并改善其功能。     

内皮祖细胞生物学特性及其进展

内皮祖细胞(Endothelial Progenitor Cells,EPCs)是血管内皮细胞的前体细胞,即能分化为成熟血管内皮细胞的祖细胞。随着对EPCs功能和影响其分化、生存、归巢和组织分布因素的了解,EPCs作为临床诊断、预后判断和治疗方法将有广阔的前景。本文就EPCs的的来源,EPCs的分离、培养、鉴定,EPCs的表面标志,EPCs的动员、分化和归巢等生物学特性及其进展展开综述。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.