Deciphering archaeological palimpsests with bone micro-fragments from the Lower Magdalenian of El Mirón cave (Cantabria,Spain)

Abstract

Modern excavation techniques aim accurately to recover extant archaeological data. Usually bone micro-fragments are gathered as a result, however, during archaeological analysis these remains are often set apart as indeterminate bones and generally do not contribute to the interpretation of the deposits. How to decipher archaeological palimpsests using these small bone fragments is the aim of this paper. El Mirón Cave, located in northern Iberia, contains a very rich Cantabrian Lower Magdalenian deposit (17–15 uncal ka BP) with high densities of faunal remains and artefacts. Here, we present zooarchaeological, taphonomic and spatial distribution analyses of macromammal finds, including those small bone fragments, accumulated during a series of intensive and repeated human occupations found in the outer vestibule excavation area. Our results show that a broad spectrum of activities was performed there, including meat, marrow and grease processing and waste abandonment. We propose that bone micro-fragments must be considered when addressing human subsistence reconstructions from animal remains, as they represent the leftovers of the chaîne opératoire of animal carcass exploitation. The archaeological implications of their inclusion are extremely valuable, especially when deciphering palimpsests. A multidisciplinary approach to study these small animal remains provides information that otherwise would be missed.     

Seasonal variation of Pythium Pringsh (Pythiacea,Oomycetes) in the irrigation reservoirs of the Poniente Almeriense (Almería,Spain)

Population density (number of propagules/l) of Pythium in the irrigation water has been studied in the reservoirs of the Poniente Almeriense (Almería, Spain). These reservoirs are habitually used in the watering of the greenhouse crops of the district. From October 1994 to August 1997, 17 sampling campaigns were carried out, 23 reservoirs were visited and three of them were sampled during three years. 300 ml of superficial water were sampled and analyzed in the laboratory by means of filtration with paper Whatman number 1, placed on Ponchet selective medium and incubated at 20 degrees C. Data of the physical-chemical characteristics of the water (temperature, pH, CE, anions and cations) were taken. The geographical and seasonal distributions of the population were correlated with the analyzed characteristics. The more frequent densities were among 0-100 propagules/l. A population's seasonality was observed, with a maximum in winter and a minimum in summer. When Cl- concentration was higher, the number of propagules/l was smaller.     

Stratigraphy and sedimentology of Muschelkalk carbonates of the Southern Iberian Continental Palaeomargin (Siles and Cehegín Formations,Southern Spain)

The Triassic sediments of the External Zones of the Betic Cordillera were deposited on the Southern Iberian Continental Palaeomargin. Two coeval Ladinian formations, namely the Siles Formation and the Cehegín Formation, are described to illustrate the facies and lithostratigraphic variability in the Muschelkalk carbonates. There has been some dispute over the number of carbonate units present in the Siles Formation. Our studies assign a tectonic origin to these recurrent carbonate units. Both formations comprise only one carbonate unit, which is correlated to the Upper Muschelkalk of the Catalan and Germanic basins and some Iberian Range sections. To characterize the sedimentological features of these formations, 14 facies were defined. The most widespread sediment was originally lime mud, although bioclastic deposits are also common. In the facies succession, a main transgressive-regressive sequence could be identified. According to the facies model proposed here, a muddy coastal and shallow-water platform prograded over mid ramp deposits. There is no evidence for a seawards reefal or oolitic-bioclastic sandy barrier. The most significant feature of this sedimentary interpretation is that these carbonate facies show clear characteristics of an epicontinental platform.     

Middle Albian gymnosperms from the Río Martín Valley (Teruel,Spain)

The fossil-rich site within the Albian Escucha Formation in the Oliete Sub-basin, located in the Northeast of Spain, has provided a great abundance of impressions, including leave remains and seed cones of Coniferales (Sphenolepis kurriana, Sphenolepis sternbergiana, Elatides curvifolia, Brachyphyllum sp. and Geinitzia sp. cf. G. rigida), leaves of Caytoniales (Sagenopteris elliptica), Ginkgoales (Ginkgoites pluripartita, and Sphenobaiera sp. cf. S. longifolia), Bennettitales (Ptilophyllum sp. cf. P. pecten and aff. Zamites sp.), Cycadales (Nilssonia tenuinervis and Ctenozamites with entire and denticulate pinnae margins), Czekanowskiales, and reproductive structures of Gnetales. The gymnosperm remains are fairly abundant in the upper member, built up of sediments deposited in fluvial and swamp environments without marine influence. The different gymnosperm associations in this outcrop indicate a wide variety of environments in a subtropical climate, and a mixture of North American and European taxa in the Iberian plate during the Middle Albian.     

Seasonal variations of pollution indicators in a wildfowl reserve (Do?ana National Park, Spain)

Pollution indicators in Do?ana National Park show a cyclic seasonal pattern with maximum level in the autumn and minimum in the spring. While faecal streptococci proved to be the best indicator micro-organisms in this type of salty, alkaline environment, total coliforms gave an overestimation of pollution because of the presence of hydroteluric coliforms.     

Bone remodelling in Neanderthal mandibles from the El Sidrón site (Asturias, Spain)

Skull morphology results from the bone remodelling mechanism that underlies the specific bone growth dynamics. Histological study of the bone surface from Neanderthal mandible specimens of El Sidrón (Spain) provides information about the distribution of the remodelling fields (bone remodelling patterns or BRP) indicative of the bone growth directions. In comparison with other primate species, BRP shows that Neanderthal mandibles from the El Sidrón (Spain) sample present a specific BRP. The interpretation of this map allows inferences concerning the growth directions that explain specific morphological traits of the Neanderthal mandible, such as its quadrangular shape and the posterior location of the mental foramen.     

Microalgae associated with deteriorated stonework of the fountain of Bibatauín in Granada,Spain

The fountain of Bibatauín, in Granada (Spain), is in a poor state of conservation, and biodeterioration is occurring. Colonization by microalgae and its effects on the fountain were investigated. The microorganisms from representative sampling areas were identified by optical microscopy, and the biogenic carbonate crusts they formed analysed by X-ray diffraction and field emission scanning electronic microscopy. The most representative genera found were Cosmarium, Phormidium and Symploca, and the main mineral was calcite.     

The spread of agriculture in northern Iberia: new archaeobotanical data from El Mirón cave (Cantabria) and the open-air site of Los Cascajos (Navarra)

This paper presents archaeobotanical results from the Neolithic levels (5,300–4,000 b.c.) of two recently excavated sites in northern Iberia: El Mirón cave (Cantabria) and the open-air site of Los Cascajos (Navarra). A cereal grain from El Mirón is currently the earliest domesticated plant remain from this region. Despite the large number of samples examined, plant remains are few. They include basically cereals (Triticum monococcum, T. dicoccum, T. aestivum/durum/turgidum and Hordeum vulgare) and some nuts and fruits (Corylus avellana, Quercus sp., Vitis sp., etc.). The presence of free-threshing wheats at El Mirón opens up an interesting subject for debate, as until now naked wheats have been absent from the early Neolithic archaeobotanical record of the coastal Cantabrian region. Hulled wheat chaff is the main plant component from Los Cascajos, south of the Cantabrian Cordillera in Navarra, indicating waste from processing activities. The association of barley almost exclusively with both a burial and a ritual vase in Los Cascajos could be related specific rituals or ceremonies.     

Chronology of the Middle to Upper Palaeolithic transition at Abric Romaní, Catalunya

This paper presents new data from Abric Romaní, a key site in the region of Catalunya, northeastern Iberia, which is central to discussions of the transition between the Middle and Upper Palaeolithic in Europe. Until now, the Mid-Upper Paleolithic transition had been dated at the site through samples from the remaining baulk sections of levels A and B (typologically classified as ‘earliest Aurignacian’ and Mousterian, respectively) at the rear of the rockshelter, which were left from excavations in the late 1900s and early 1910s. We dated samples of bone and charcoal from these remnant sections with accelerator mass spectrometry (AMS) methods. We also analysed several humanly-modified artefacts (bone points and perforated shells) excavated from other areas of the same layers. From the initial series, we obtained ages of c. 20 ka BP (thousands of years before present); much younger than expected if they indeed dated to the early Upper Palaeolithic. We sampled additional material to test the robustness of these initial ages, and older determinations that were more comparable with the chronology outlined by [Bischoff et?al., 1988] and [Bischoff et?al., 1994] resulted. All of the old and new results have been compared in a Bayesian model using the new INTCAL09 ¹⁴C calibration dataset. The results appear to confirm the suggestion of some researchers (e.g., Zilhão and d’Errico, 1999) that there was no Aurignacian in the north of Iberia until c. 36,500 BP. The chronometric model shows a good level of agreement between the radiocarbon and U-series chronologies previously obtained, and the new results published in this paper.     

Airborne pollen and spores of León (Spain)

A qualitative and quantitative analysis of airborne pollen and spores was carried out over 2 years (from September 1987 to August 1989) in the city of León. Slides were prepared daily using a volumetric pollen trap, which was placed on the Faculty of Veterinary Science building (University of León) 12m above ground-level. Fifty-one pollen types were observed; the most important of these were: Cupressaceae during the winter,Pinus andQuercus in spring, and Poaceae, Leguminosae and Chenopodiaceae in the summer. The results also showed the existence of a rich mould spore assemblage in the atmosphere. The group of Amerospores (Penicillium, Aspergillus andCladosporium) as well as Dictyospores (Alternaria) were the most abundant;Puccinia was common in the air in August. Fluctuations in the total pollen and spores m³ of air were compared with meteorological parameters (temperature, relative humidity and rainfall). From the daily sampling of the atmosphere of León, considering the maximum and minimum temperature and duration of rainfall, the start of the pollen grain season was observed generally to coincide with a rise in temperature in the absence of rain.     

Metal Accumulation Screening of the Río Tinto Flora (Huelva,Spain)

Río Tinto (Huelva, Spain) is located in one of the most important mining regions in the world. Its soils are characterized by their extreme acidity and elevated concentrations of heavy metals. Due to these characteristics, the Tinto ecosystem is considered unique and an ideal location to study biological adaptations to this type of habitat. Plant species that present these adaptations might be useful to mining and other metal pollution restoration programs. This study reports the results for the screening of Ca, Mg, Na, Mn, Fe, Ni, Cu, Zn, As, and Pb in aerial tissues of 97 plant species from the Tinto basin flora. In addition, plant–soil relationships were analyzed using the biological absorption coefficient (BAC) to detect the main plant adaptations in the Tinto flora. The species selected are representative of the biomass of the main dominant edaphophile and climatophile vegetation communities of the three river sections, forest, and subseral stages. Plant and soil elemental analyses were performed using inductively coupled plasma–mass spectrometry technique (ICP-MS). The results indicate that in general, Tinto flora shows a pattern of accumulation of the analyzed elements in aerial tissues which agrees with the nutritional requirements of vascular plants (macronutrients > micronutrients > indifferent or toxic elements). Among macronutrients, Ca seems to be an essential element in this habitat. This element accumulates in the aerial plant tissues. Basically, the Río Tinto flora is made of Fe, Cu, Zn, Ni, As, and Pb excluders, although some analyzed species of Erica, Quercus, Lavandula, Cistus, Genista, and Cytisus genera can be considered Mn accumulators. The results of this study make up a body of fundamental knowledge of the strategies used by plants to thrive in habitats with high concentrations of toxic heavy metals. This information is vital when it comes to planning a restoration program. Plants must be selected and used according to the requirements, always respecting the characteristics of the territory and facilitating the development of suitable vegetation.     

Possible Further Evidence of Low Genetic Diversity in the El Sidrón (Asturias,Spain) Neandertal Group: Congenital Clefts of the Atlas

We present here the first cases in Neandertals of congenital clefts of the arch of the atlas. Two atlases from El Sidrón, northern Spain, present respectively a defect of the posterior (frequency in extant modern human populations ranging from 0.73% to 3.84%), and anterior (frequency in extant modern human populations ranging from 0.087% to 0.1%) arch, a condition in most cases not associated with any clinical manifestation. The fact that two out of three observable atlases present a low frequency congenital condition, together with previously reported evidence of retained deciduous mandibular canine in two out of ten dentitions from El Sidrón, supports the previous observation based on genetic evidence that these Neandertals constituted a group with close genetic relations. Some have proposed for humans and other species that the presence of skeletal congenital conditions, although without clinical significance, could be used as a signal of endogamy or inbreeding. In the present case this interpretation would fit the general scenario of high incidence of rare conditions among Pleistocene humans and the specific scenariothat emerges from Neandertal paleogenetics, which points to long-term small and decreasing population size with reduced and isolated groups. Adverse environmental factors affecting early pregnancies would constitute an alternative, non-exclusive, explanation for a high incidence of congenital conditions. Further support or rejection of these interpretations will come from new genetic and skeletal evidence from Neandertal remains.     

Annual,daily and dirunal variations of Urticaceae airborne pollen in Málaga (Spain)

In the Mediterranean area, Urticaceae pollen, together with the pollen of olive and grasses, are the aeroallergens with the highest incidence in the population. From October 1991 to September 1993, with the aid of a Burkard spore-trap, we carried out a study on the Urticaceae pollen content in the atmosphere of Málaga, a seaside resort situated in the Costa del Sol (southern Spain). In Málaga, the Urticaceae pollen season is very long and their pollen grains are detected throughout the year. However, peaks were recorded in March and April and the variables most influencing concentration were maximum air temperature, sunshine hours and relative humidity. Diurnal patterns show that peaks occur generally from 10:00 h to 16:00 h when the temperature reaches its highest values.     

Annual,daily and diurnal variations of Urticaceae airborne pollen in Málaga (Spain)

In the Mediterranean area, Urticaceae pollen, together with the pollen of olive and grasses, are the aeroallergens with the highest incidence in the population. From October 1991 to September 1993, with the aid of a Burkard spore-trap, we carried out a study on the Urticaceae pollen content in the atmosphere of Málaga, a seaside resort situated in the Costa del Sol (southern Spain). In Málaga, the Urticaceae pollen season is very long and their pollen grains are detected throughout the year. However, peaks were recorded in March and April and the variables most influencing concentration were maximum air temperature, sunshine hours and relative humidity. Diurnal patterns show that peaks occur generally from 10:00 h to 16:00 h when the temperature reaches its highest values.     

Ecology,morphology and physiology of Chroothece richteriana (Rhodophyta,Stylonematophyceae) in the highly calcareous Río Chícamo,south-east Spain

The ecology of Chroothece was studied in the highly calcareous Río Chícamo, south-east Spain, in order to explain its success there, but rarity elsewhere. The river, which originates mainly from an underground aquifer, has water with high conductivity, sulphate and nitrate but low phosphate concentrations, the latter mainly organic. Chroothece occurs in mats and in lobed colonies reaching 4 cm in the broadest dimension. The colony surface consists of one layer of cells, each of which is attached to a stalk, which dichotomizes when the cell divides; stalks often extend to the colony base. The central region of many mat cells and almost all colony cells has a yellow to orange-brown colour, associated with the numerous lipid droplets densely covering the surface of the pyrenoid and arms of the star-shaped chloroplast. Field material and laboratory isolates indicate that stalk formation occurs under moderate P limitation and both stalks and cell sheath show high phosphatase activities. This also occurred in a culture collection strain maintained for 30 years in a very P-rich medium, but then transferred to a moderately P-limiting medium (c. 0.9 mg l^?1). We suggest that colony formation is initiated by aggregation of motile cells following P pulses in the water. Comparisons are made with Rivularia, a competitor in this nitrate-rich river, in spite of being a N₂-fixer. One difference is that Chroothece cells lie at the periphery of the colonies and are therefore exposed to maximum sunlight, whereas Rivularia trichomes grow inside colonies with photoprotection by scytonemin. However, the ability to withstand heavy grazing pressure may be an especially important factor favouring Rivularia here.     

Seasonal morphophysiological variations in the prostatic complex of the Tarabul’s gerbil (Gerbillus tarabuli)

Gerbillus tarabuli is a nocturnal Saharan rodent which has an annual reproductive cycle characterized by the reproductive activity in spring and a long phase of sexual quiescence in other seasons. We describe the morphology and hormonal regulation of the prostatic complex of this rodent in the two periods, based on anatomical, histological, morphometric, and immunohistochemical analyses. The organisation of this prostatic complex is similar to that reported for Meriones unguiculatus, but different from the prostate of Psammomys obesus, the rat, and the mouse. In addition to the anterior lobes, ventral lobes, and dorsal lobes, the prostatic complex of Gerbillus tarabuli, also includes dorsolateral lobe. Each lobe is composed of a fibro-muscular stroma surrounding a glandular epithelium. Dorsolateral lobes are easily distinguishable by their big volume. The prostate grows and regresses cyclically throughout the year. During the resting season, ventral lobes and anterior lobes showed atrophy, with a significant decrease in both epithelial height and supranuclear area size, and a strong thickening of the fibro-muscular compartment. In dorsal lobes, the epithelial and stromal compartments atrophied and regenerated simultaneously, whereas in dorsolateral lobe the thickness of the epithelium, the supranuclear zone and the stroma increased during resting period. Furthermore, seasonal variations were observed in the distribution and expression of both androgen receptors, and estrogens receptors. Expression patterns of all receptors were lobe-specific. In conclusion, both androgens and estrogens are involved in the homeostasis and regulation of the prostate in Gerbillus tarabuli. Dorsolateral lobe seems to be controlled by a different mechanism than other lobes.     

Assemblages of peracarid crustaceans in subtidal sediments from the Ría de Aldán (Galicia,NW Spain)

Peracarid crustaceans inhabit many marine benthic habitats and are good indicators of environmental conditions. There is, however, a lack of information about diversity and distribution of peracarid crustaceans on the shallow subtidal sediments of the Galician rias. In the summer of 1997, 27 subtidal stations were sampled in the Ría de Aldán, a ria on the southern margin of the mouth of the Ría de Pontevedra (Galicia, NW Spain). A total of 16,191 peracarid individuals were collected, comprising 125 species belonging to five orders. Amphipods were dominant in number of species and individuals, followed by isopods and cumaceans. Multivariate analyses of these data indicated that depth and sediment granulometry were major determinants of distribution and composition of peracarid assemblages in the ria.