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Identification of Jasmonic Acid in Three Species of Higher Plants and Its Biological Activities 总被引:2,自引:0,他引:2
Yamane Hisakazu; Takagi Hideo; Abe Hisato; Yokota Takao; Takahashi Nobutaka 《Plant & cell physiology》1981,22(4):689-697
()-Jasmonic acid (JA) was isolated and characterizedfrom immature seeds of Phaseolus vulgaris L. as a growth inhibitoragainst rice seedlings. The presence of JA was also confirmedin both fresh leaves and insect galls of Castanea crenata Sieb.et Zucc. and immature seeds of Dolichos lablab L. by combinedgas chromatography-mass spectrometry analyses. The biologicalactivities of JA were evaluated in several bioassay systems,and it was shown that JA is a new type of plant growth regulator. (Received February 9, 1981; Accepted March 27, 1981) 相似文献
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During the imbibition of seeds in polyethyleneglycol (PEG),increasing amounts of ATP accumulated up to 24 h. Similar amountsaccumulated in the seeds during 45 h of imbibition inwater. Radioactive amino acids were increasingly incorporatedin the acid-insoluble fraction up to 24 h imbibition in PEG,as well as in water, after which a sharp decrease occurred upto 5 d of imbibition. If seeds were imbibed in PEG or waterin the presence of radioactive acetate, water-insoluble radioactivityincreased linearly in seeds during 5 d of imbibition. The amountsof incorporated amino acids or acetate were about double inPEG-imbibed as compared with in water-imbibed seed. The incorporationof AMP into the acid-insoluble fraction in seeds imbibed inPEG in the presence of radioactive AMP levelled off after 24h followed by a sharp decrease of up to 10% of the peak 5 dafter the start of imbibition. In water-imbibed seeds the incorporationof AMP continued to increase during at least 5 d of imbibition.During redrying of PEG-treated seeds (24 h), at least 80% ofthe accumulated ATP decreased during 18 d. The total radioactiveamino acids and nucleotide decreased during 3 d of redryingby 20% and 60%, respectively. At that time, the acid-insolubleincorporates increased by 20% and 50%, respectively. Some ofthe AMP was released as CO2. Key words: AMP, Germination, Nucleic acid synthesis, Osmoconditioning, PEG, Protein synthesis 相似文献
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L. M. Bartova V. P. Bogdanov T. S. Kalashnikova N. N. Kulagina O. S. Reshetova V. L. Sadovskaya V. D. Voblikova G. S. Muromtsev 《Journal of Plant Growth Regulation》1997,16(2):109-113
Fusicoccin-like ligands in higher plants were sought by combining high performance liquid chromatography with radioreceptor
analysis and radioimmunoassay. Such substances were found in genetically transformed cultured roots of horseradish, alteus
and lupine as well as in native horseradish, cucumber, horse chestnut, and maize plants. In root crops such as carrot and
sugar beet or in potato tubers, only traces of fusicoccin-like ligands were detected. Fusicoccin A was detected in genetically
transformed cultured roots of horseradish by gas chromatography/mass spectrometry.
Received October 15, 1996; accepted February 14, 1997 相似文献
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In pancreatic β-cells, K,ATP channels respond to changes in glucose to regulate cell excitability and insulin release. Confirming a high sensitivity of electrical activity to K,ATP activity, mutations that cause gain of K,ATP function cause neonatal diabetes. Our aim was to quantitatively assess the contribution of K,ATP current to the regulation of glucose-dependent bursting by reproducing experimentally observed changes in excitability when K,ATP conductance is altered by genetic manipulation. A recent detailed computational model of single cell pancreatic β-cell excitability reproduces the β-cell response to varying glucose concentrations. However, initial simulations showed that the model underrepresents the significance of K,ATP activity and was unable to reproduce K,ATP conductance-dependent changes in excitability. By altering the ATP and glucose dependence of the L-type Ca2+ channel and the Na-K ATPase to better fit experiment, appropriate dependence of excitability on K,ATP conductance was reproduced. Because experiments were conducted in islets, which contain cell-to-cell variability, we extended the model from a single cell to a three-dimensional model (10×10×10 cell) islet with 1000 cells. For each cell, the conductance of the major currents was allowed to vary as was the gap junction conductance between cells. This showed that single cell glucose-dependent behavior was then highly variable, but was uniform in coupled islets. The study highlights the importance of parameterization of detailed models of β-cell excitability and suggests future experiments that will lead to improved characterization of β-cell excitability and the control of insulin secretion. 相似文献
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In pancreatic β-cells, K,ATP channels respond to changes in glucose to regulate cell excitability and insulin release. Confirming a high sensitivity of electrical activity to K,ATP activity, mutations that cause gain of K,ATP function cause neonatal diabetes. Our aim was to quantitatively assess the contribution of K,ATP current to the regulation of glucose-dependent bursting by reproducing experimentally observed changes in excitability when K,ATP conductance is altered by genetic manipulation. A recent detailed computational model of single cell pancreatic β-cell excitability reproduces the β-cell response to varying glucose concentrations. However, initial simulations showed that the model underrepresents the significance of K,ATP activity and was unable to reproduce K,ATP conductance-dependent changes in excitability. By altering the ATP and glucose dependence of the L-type Ca2+ channel and the Na-K ATPase to better fit experiment, appropriate dependence of excitability on K,ATP conductance was reproduced. Because experiments were conducted in islets, which contain cell-to-cell variability, we extended the model from a single cell to a three-dimensional model (10×10×10 cell) islet with 1000 cells. For each cell, the conductance of the major currents was allowed to vary as was the gap junction conductance between cells. This showed that single cell glucose-dependent behavior was then highly variable, but was uniform in coupled islets. The study highlights the importance of parameterization of detailed models of β-cell excitability and suggests future experiments that will lead to improved characterization of β-cell excitability and the control of insulin secretion. 相似文献
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高等植物病原相关蛋白 总被引:4,自引:0,他引:4
在过去的三十年中,人们对诱导系统性抗性——这一普遍存在于高等植物抗病过程中的现象——进行了深入研究。被真菌、细菌或病毒侵染后,植物表现出广泛的、长时间的系统性抗性。在这一过程中,植物细胞壁组成成分发生改变,表达各种病原相关蛋白(PR蛋白),并合成多种植物抗毒素。本文就主要的PR蛋白家族的结构和功能特性,PR蛋白的发现和分类,及PR蛋白的应用作一综述。 相似文献
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高等植物中的多肽激素 总被引:1,自引:0,他引:1
高等植物的第一个多肽激素(系统素)发现已经有10多年的历史。到目前为止, 被普遍认可的植物多肽激素有4种: 系统素、PSK、CLV3和SCR, 分别参与了植物的防御反应、细胞的分裂、茎端生长点干细胞数目维持和花粉-柱头的识别过程。这些小分子多肽化合物以配基的形式与细胞膜表面的受体激酶相互作用, 从而实现细胞之间的信号交流。本文对这4种多肽激素及其相应受体的研究进展做了简要评述, 并着重介绍当前研究比较热门的CLV3多肽, 最后对相关领域的发展前景进行探讨。 相似文献
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