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1.
1000-Grain weight and spikelet number per panicle are two important components for rice grain yield.In our previous study,eight quantitative trait loci(QTLs)conferring spikelet number per panicle and 1000-grain weight were mapped through sequencing-based genotyping of 150 rice recombinant inbred lines(RILs).In this study,we validated the effects of four QTLs from Nipponbare using chromosome segment substitution lines(CSSLs),and pyramided eight grain yield related QTLs.The new lines containing the eight QTLs with positive effects showed increased panicle and spikelet size as compared with the parent variety 93-11.We further proposed a novel pyramid breeding scheme based on marker-assistant and phenotype selection(MAPS).This scheme allowed pyramiding of as many as 24 QTLs at a single hybridization without massive cross work.This study provided insights into the molecular basis of rice grain yield for direct wealth for high-yielding rice breeding. 相似文献
2.
Quantitative Trait Loci Mapping for ChlorophyⅡ Fluorescence and Associated Traits in Wheat ( Triticum aestivum) 总被引:10,自引:0,他引:10
De-Long Yang Rui-Lian Jing Xiao-Ping Chang Wei Li 《植物学报(英文版)》2007,49(5):646-654
Parameters of chlorophyll fluorescence kinetics (PCFKs) under drought stress condition are generally used to characterize instincts for dehydration tolerance in wheat (Triticum aestivum L.). Therefore, it is important to map quantitative trait loci (QTLs) for PCFKs in wheat genetic improvement for drought tolerance. A doubled haploid (DH) population with 150 lines, derived from a cross between two common wheat varieties, Hanxuan 10 and Lumai 14, was used to analyze the correlation between PCFKs and chlorophyll content (CHIC) and to map QTLs at the grainfilling stage under conditions of both rainfed (drought stress, DS) and well-watered (WW), respectively. QTLs for these traits were detected by QTLMapper version 1.0 based on the composite Interval mapping method of the mixed-linear model. The results showed a very significant positive correlation between Fv, Fm, Fv/Fm and Fv/Fo. The correlation coefficients were generally higher under WW than under DS. Also, there was a significant or a highly significant positive correlation between Fv, Fm, Fv/Fm, Fv/Fo and CHIC. The correlation coefficients were higher in the DS group than the WW group. A total of 14 additive QTLs (nine QTLs detected under DS and five QTLs under WW) and 25 pairs of eplstatlc QTLs (15 pairs detected under DS and 10 pairs under WW) for PCFKs were mapped on chromosomes 6A, 7A, 1B, 3B, 4D and 7D. The contributions of additive QTLs for PCFKs to phenotype variation were from 8.40% to 72.72%. Four additive QTLs (two QTLs detected under DS and WW apiece) controlling Chic were mapped on chromosomes 1A, 5A and 7A. The contributions of these QTLs for ChIC to phenotype variation were from 7.27% to 11.68%. Several QTL clusters were detected on chromosomes 1B, 7A and 7D, but no shared chromosomal regions for them were identified under different water regimes, indicating that these QTLs performed different expression patterns under rainfed and well-watered conditions. 相似文献
3.
Quantitative trait loci for yield and yield components in an Oryza sativa×Oryza rufipogon BC2F2 population evaluated in an upland environment 总被引:1,自引:0,他引:1
P. Moncada C. P. Martínez J. Borrero M. Chatel H. Gauch Jr E. Guimaraes J. Tohme S. R. McCouch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(1):41-52
An advanced backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with eight agronomic
traits in a BC2F2 population derived from an interspecific cross between Caiapo, an upland Oryza sativa subsp. japonica rice variety from Brazil, and an accession of Oryza rufipogon from Malaysia. Caiapo is one of the most-widely grown dryland cultivars in Latin America and may be planted as a monoculture
or in a multicropping system with pastures. The objectives of this study were: (1) to determine whether trait-enhancing QTLs
from O. rufipogon would be detected in 274 BC2F2 families grown under the drought-prone, acid soil conditions to which Caiapo was adapted, (2) to compare the performance
with and without pasture competition, and (3) to compare putative QTL-containing regions identified in this study with those
previously reported for populations adapted to irrigated, low-land conditions. Based on analyses of 125 SSLP and RFLP markers
distributed throughout the genome and using single-point, interval, and composite interval mapping, two putative O. rufipogon derived QTLs were detected for yield, 13 for yield components, four for maturity and six for plant height.We conclude that
advanced backcross QTL analysis offers a useful germplasm enhancement strategy for the genetic improvement of cultivars adapted
to stress-prone environments. Although the phenotypic performance of the wild germplasm would not suggest its value as a breeding
parent, it is noteworthy that 56% of the trait-enhancing QTLs identified in this study were derived from O. rufipogon. This figure is similar to the 51% of favorable QTLs derived from the same parent in crosses with a high-yielding hybrid
rice cultivar evaluated under irrigated conditions in a previous study. In conclusion, parallel studies in rice using AB-QTL
analysis provide increasing evidence that certain regions of the rice genome are likely to harbor genes of interest for plant
improvement in multiple environments.
Received: 3 September 1999 / Accepted: 16 May 2000 相似文献
4.
Song-Ping Hu ;Ying Zhou ;Lin Zhang ;Xiu-Dong Zhu ;Lin Li ;Li-Jun Luo ;Guo-Lan Liu ;Qing-Ming Zhou 《Acta Botanica Sinica》2009,(9):879-888
In order to explore the relevant molecular genetic mechanisms of photosynthetic rate (PR) and chlorophyll content (CC) in rice (Oryza sativa L.), we conducted a series of related experiments using a population of recombinant inbred lines (Zhenshan97B x IRAT109). We found a significant correlation between CC and PR (R = 0.19**) in well-watered conditions, but no significant correlation during water stress (r = 0.08). We detected 13 main quantitative trait loci (QTLs) located on chromosomes 1, 2, 3, 4, 5, 6, and 10, which were associated with CC, including six QTLs located on chromosomes 1, 2, 3, 4, and 5 during water stress, and seven QTLs located on chromosomes 2, 3, 4, 6, and 10 in well-watered conditions. These QTLs explained 47.39% of phenotypic variation during water stress and 56.19% in well-watered conditions. We detected four main QTLs associated with PR; three of them (qPR2, qPR10, qPR11) were located on chromosomes 2, 10, and 11 during water stress, and one (qPR10) was located on chromosome 10 in well-watered conditions. These QTLs explained 34.37% and 18.41% of the phenotypic variation in water stress and well-watered conditions, respectively. In total, CC was largely controlled by main QTLs, and PR was mainly controlled by epistatic QTL pairs. 相似文献
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6.
Sujuan Li Song Yan A-hong Wang Guihua Zou Xuehui Huang Bin Han Qian Qian Yuezhi Tao 《Plant cell reports》2013,32(1):103-116
Key message
The performance of callus induction and callus differentiation was evaluated by 9 indices for 140 RILs; 2 major QTLs associated with plant regeneration were identified.Abstract
In order to investigate the genetic mechanisms of tissue culture response, 140 recombinant inbred lines (RILs) derived from 93-11 (Oryza sativa ssp. indica) × Nipponbare (Oryza sativa ssp. japonica) and a high quality genetic map based on the SNPs generated from deep sequencing of the RIL genomes, were used to identify the quantitative trait loci (QTLs) associated with in vitro tissue culture response (TCR) from mature seed in rice. The performance of callus induction was evaluated by indices of induced-callus color (ICC), induced-callus size (ICS), induced-callus friability (ICF) and callus induction rate (CIR), respectively, and the performance of callus differentiation was evaluated by indices of callus proliferation ability (CPA), callus browning tendency (CBT), callus greening ability (CGA), the average number of regenerated shoots per callus (NRS) and regeneration rate (%, RR), respectively. A total of 25 QTLs, 2 each for ICC, ICS, ICF, CIR and CBA, 3 for CPA, 4 each for CGA, NRS and RR, respectively, were detected and located on 8 rice chromosomes. Significant correlations were observed among the traits of CGA, NRS and RR, and QTLs identified for these three indices were co-located on chromosomes 3 and 7, and the additive effects came from both Nipponbare and 93-11, respectively. The results obtained from this study provide guidance for further fine mapping and gene cloning of the major QTL of TCR and the knowledge of the genes underlying the traits investigated would be very helpful for revealing the molecular bases of tissue culture response. 相似文献7.
Isaac Kofi Bimpong Rachid Serraj Joong Hyoun Chin Joie Ramos Evelyn M. T. Mendoza Jose E. Hernandez Merlyn S. Mendioro Darshan S. Brar 《Journal of Plant Biology》2011,54(4):237-250
Drought is a major abiotic stress that limits rice productivity in rain-fed and upland ecosystems. African rice, Oryza glaberrima, has low yields but is tolerant to drought and other stresses. We evaluated 513 BC2F3 progenies from alien introgression lines (AILs) that were derived from crosses of Oryza sativa (IR64) × O. glaberrima. They were assessed for yield and other traits when grown under drought at two locations. Such conditions reduced grain production
by 59% compared with the recurrent parent (IR64). However, 33 AILs had higher yields, thus demonstrating their potential as
genetic material for transferring drought-related traits from O. glaberrima to O. sativa. A set of 200 AILs was selectively genotyped with 173 simple sequence repeat and sequenced tagged site markers. Molecular
analysis showed that a mean of 4.5% of the O. glaberrima genome was introgressed in BC2F3 AILs. Our analysis revealed 33 quantitative trait loci (QTLs; including 10 novel) for different traits. O. glaberrima contributed 50% of the alleles to those newly identified QTLs, with one for grain yield per plant (ypp9.1) being new. A QTL at RM208 on chromosome 2 positively affected yield under stress, accounting for 22% of the genetic variation.
Our identification of drought-related QTLs for yield and yield components will be useful to future research efforts in marker-assisted
selection. 相似文献