共查询到20条相似文献,搜索用时 93 毫秒
1.
2.
3.
4.
5.
Stapleton M Carlson J Brokstein P Yu C Champe M George R Guarin H Kronmiller B Pacleb J Park S Wan K Rubin GM Celniker SE 《Genome biology》2002,3(12):research0080.1-808
6.
VdNEP, an Elicitor from Verticillium dahliae, Induces Cotton Plant Wilting 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jian-Ying Wang Yu Cai Jin-Ying Gou Ying-Bo Mao Yan-Hua Xu Wei-Hong Jiang Xiao-Ya Chen 《Applied microbiology》2004,70(8):4989-4995
Verticillium wilt is a vascular disease of cotton. The causal fungus, Verticillium dahliae, secretes elicitors in culture. We have generated ~1,000 5′-terminal expressed sequence tags (ESTs) from a cultured mycelium of V. dahliae. A number of ESTs were found to encode proteins harboring putative signal peptides for secretion, and their cDNAs were isolated. Heterologous expression led to the identification of a protein with elicitor activities. This protein, named V. dahliae necrosis- and ethylene-inducing protein (VdNEP), is composed of 233 amino acids and has high sequence identities with fungal necrosis- and ethylene-inducing proteins. Infiltration of the bacterially expressed His-VdNEP into Nicotiana benthamiana leaves resulted in necrotic lesion formation. In Arabidopsis thaliana, the fusion protein also triggered production of reactive oxygen species and induced the expression of PR genes. When added into suspension cultured cells of cotton (Gossypium arboreum), the fusion protein elicited the biosynthesis of gossypol and related sesquiterpene phytoalexins at low concentrations, and it induced cell death at higher concentrations. On cotton cotyledons and leaves, His-VdNEP induced dehydration and wilting, similar to symptoms caused by a crude preparation of V. dahliae elicitors. Northern blotting showed a low level of VdNEP expression in the mycelium during culture. These data suggest that VdNEP is a wilt-inducing factor and that it participates in cotton-V. dahliae interactions. 相似文献
7.
Vincentz M Cara FA Okura VK da Silva FR Pedrosa GL Hemerly AS Capella AN Marins M Ferreira PC França SC Grivet L Vettore AL Kemper EL Burnquist WL Targon ML Siqueira WJ Kuramae EE Marino CL Camargo LE Carrer H Coutinho LL Furlan LR Lemos MV Nunes LR Gomes SL Santelli RV Goldman MH Bacci M Giglioti EA Thiemann OH Silva FH Van Sluys MA Nobrega FG Arruda P Menck CF 《Plant physiology》2004,134(3):951-959
8.
The anthracnose resistance locus Co-4 of common bean is located on chromosome 3 and contains putative disease resistance-related genes 总被引:1,自引:0,他引:1
Melotto M Coelho MF Pedrosa-Harand A Kelly JD Camargo LE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(4):690-699
9.
10.
11.
12.
13.
Ospina-Giraldo MD Collopy PD Romaine CP Royse DJ 《Fungal genetics and biology : FG & B》2000,29(2):81-94
Two complementary DNA (cDNA) libraries were constructed from tissues isolated from primordia and basidiomes of Agaricus bisporus to characterize genes involved in mushroom development. Using single-pass sequencing of 869 cDNA clones, we found 477 expressed sequence tags (ESTs), including 466 not previously described in the databases for A. bisporus. A BLASTX search revealed that 374 ESTs had similarities with protein sequences available from databases; 193 of these ESTs were categorized according to their putative function. Most ESTs were assigned to one of four roles: metabolism (23%), cell structure (15%), cell growth and division (12%), and protein destination and storage (10%). The remaining ESTs with putative homologues were classified in 10 additional categories. Many ESTs could not be functionally assigned. Based on redundancy levels, at least 4 ESTs were preferentially expressed in each tissue type. Sequence analysis also suggested the presence of paralog tyrosinase genes in the A. bisporus genome. 相似文献
14.
Jang-Seu Ki Young-Mi Lee Sang-Oun Jung Toshihiro Horiguchi Hyeon-Seo Cho Jae-Seong Lee 《Molecular phylogenetics and evolution》2010,54(3):1016-1020
Class Gastropoda includes a large number of described species, many with extensively rearranged mitochondrial genomes. We sequenced the mitogenome of the rock shell, Thais clavigera (Gastropoda: Muricidae), an intertidal snail, using long PCR with primers designed on the basis of expressed sequence tags. The mitogenome of T. clavigera consists of 2 rRNAs, 22 tRNAs, and 13 protein-coding genes, but no control region. Structural comparisons revealed that the order Sorbeoconcha, including T. clavigera, have nearly identical mitochondrial gene patterns. However, they have an inversion between a tRNAPhe–tRNAGlu cluster that comprises 21 genes, but most of the remaining structure is similar to the putative mollusk ground pattern. These findings will provide a better insight into mitochondrial gene rearrangement over the course of gastropod evolution. 相似文献
15.
A total of 1000 expressed sequence tags (ESTs) corresponding to 760 unique sequence sets were identified using random sequencing of clones from a cDNA library constructed from mycelial RNA of Phytophthora infestans. A number of software programs, represented by a relational database and an analysis pipeline, were developed for the automated analysis and storage of the EST sequence data. A set of 419 nonredundant sequences, which correspond to a total of 632 ESTs (63.2%), were identified as showing significant matches to sequences deposited in public databases. A putative cellular identity and role was assigned to all 419 sequences. All major functional categories were represented by at least several ESTs. Four novel cDNAs containing sequences related to elicitins, a family of structurally related proteins that induce the hypersensitive response and condition avirulence of P. infestans on Nicotiana plants, were among the most notable genes identified. Two of these elicitin-like cDNAs were among the most abundant cDNAs examined. The set also contained several ESTs with high sequence similarity to unique plant genes. 相似文献
16.
17.
18.
Two expressed sequence tags were isolated from a porcine skeletal muscle cDNA library and identified as the putative partial cDNAs of the porcine Na+ , K+ -ATPase subunit α 2 ( ATP1A2 ) and muscle phosphofructokinase ( PFKM ) genes after sequencing and homology search. Results of analysis of a pig–rodent somatic cell hybrid panel by PCR allowed the assignments of ATP1A2 to porcine chromosome (chr) 4 and of PFKM to porcine chr 5. These assignments support previously observed conservation of syntenic relationships between human chr 1 and porcine chr 4 and between human chr 12 and porcine chr 5. 相似文献
19.
Kiyoko Taniai Chikara Hirayama Kazuei Mita Kiyoshi Asaoka 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2014,184(7):827-834
Four glycine-rich protein (GRP) genes were identified from expressed sequence tags of the maxillary galea of the silkworm. All four genes were expressed in the maxillary pulp, antenna, labrum, and labium, but none of the genes were expressed in most internal organs. Expression of one of the genes, termed bmSIGRP, was further increased approximately fivefold in the mouth region (including the maxilla, antenna, labrum, labium, and mandible) after 24 h of starvation. bmSIGRP expression peaked at 24 h and gradually declined during the subsequent 2 days. When a synthetic diet not containing proteins was fed, bmSIGRP expression increased significantly in the mouth region to levels similar to that observed in starved larvae. Synthetic diets that lacked vitamins or salts but contained amino acids did not significantly affect bmSIGRP expression. These results suggest that amino acid depletion increases bmSIGRP expression. 相似文献
20.
Holly L Williams Rona N Sturrock Muhammad A Islam Craig Hammett Abul K M Ekramoddoullah Isabel Leal 《BMC genomics》2014,15(1)