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1.
Cold tolerance and dehydration in Enchytraeidae from Svalbard 总被引:4,自引:1,他引:3
L. Sømme T. Birkemoe 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(4):264-269
When cooled in contact with moisture, eight species of arctic Enchytraeidae from Svalbard were killed by freezing within
minutes or hours at −3 and −5 °C; an exception was Enchytraeus kincaidi which survived for up to 2 days. When the temperature approached 0 °C the enchytraeids apparently tried to escape from the
moist soil. The supercooling capacity of the enchytraeids was relatively low, with mean supercooling points of −5 to −8 °C.
In contrast, specimens of several species were extracted from soil cores that had been frozen in their intact state at −15 °C
for up to 71 days. Compared to freezing in a moist environment, higher survival rates were obtained during cooling at freezing
temperatures in dry soil. Survival was recorded in species kept at −3 °C for up to 35 days, and in some species kept at −6 °C
for up to 17 days. Slow warming greatly increased survival rates at −6 °C . The results strongly suggest that arctic enchytraeids
avoid freezing by dehydration at subzero temperatures. In agreement with this, weight losses of up to ca. 42% of fresh weight
were recorded in Mesenchytraeus spp. and of up to 55% in Enchytraeus kincaidi at water vapour pressures above ice at −3 to −6 °C. All specimens survived dehydration under these conditions.
Accepted: 12 December 1997 相似文献
2.
The effect of consuming terrestrial algae on the cold tolerance of two Antarctic micro-arthropods was examined. From the
results of preferential feeding experiments, seven species of Antarctic terrestrial micro-algae were chosen and fed to two
common, freeze-avoiding Antarctic micro-arthropods: the springtail Cryptopygus antarcticus (Collembola: Isotomidae), and the mite Alaskozetes antarcticus (Acari: Oribatida). Mites were very selective in their choice of food whereas the springtails were less discriminating. The
ice nucleating activity of each species of alga was measured using an ice nucleator spectrometer and a differential scanning
calorimeter. Pure cultures of individual species of algae had characteristic supercooling points ranging from ca. −5 to −18 °C.
The effect of eating a particular alga on the supercooling point of individual micro-arthropods cultured at two different
temperatures (0 and 10 °C) was examined. Neither species showed a preference for algae with low ice-nucleating activity and
there was no clear correlation between the supercooling point of food material and that of the whole animal. However, feeding
on certain algae such as Prasiola crispa, which contained the most active ice nucleators, decreased the cold tolerance of both species of arthropods.
Accepted: 6 May 2000 相似文献
3.
J. P. Costanzo J. B. Moore R. E. Lee Jr. P. E. Kaufman J. A. Wyman 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(3):169-176
This investigation examined the influence of soil moisture and associated parameters on the cold hardiness of the Colorado
potato beetle (Leptinotarsa decemlineata Say), a temperate-zone species that overwinters in terrestrial burrows. The body mass and water content of adult beetles
kept in sand at 4 °C varied over a 16-week period of diapause according to the substratum's moisture content. Changes in body
water content, in turn, influenced the crystallization temperature (range −3.3 to −18.4 °C; n = 417), indicating that environmental moisture indirectly determined supercooling capacity, a measure of physiological cold
hardiness. Beetles held in dry sand readily tolerated a 24-h exposure to temperatures ranging from 0° to −5 °C, but those
chilled in sand containing as little as 1.7% water (dry mass) had elevated mortality. Thus, burrowing in dry soils not only
promotes supercooling via its effect on water balance, but may also inhibit inoculative freezing. Mortality of beetles exposed
to −5 °C for 24 h was lower in substrates composed of sand, clay and/or peat (36–52%) than in pure silica sand (78%) having
an identical water content (17.0% dry mass). In addition to moisture, the texture, structure, water potential, and other physico-chemical
attributes of soil may strongly influence the cold hardiness and overwintering survival of burrowing insects.
Accepted: 10 September 1996 相似文献
4.
T. M. Olsen J. G. Duman 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(2):105-113
Freeze-avoiding fire-colored beetle larvae, Dendroides canadensis, were monitored seasonally to explore the role of endogenous hemolymph ice nucleators and antifreeze proteins on the maintenance
of supercooling. In preparation for overwintering, D. canadensis depressed hemolymph ice nucleator activity and increased thermal hysteresis activity [mean value circa 0. 5 °C (summer) versus
circa 5 °C (midwinter)] resulting in decreased larval and hemolymph supercooling points [−7 °C (summer) versus −20 °C (midwinter)].
Results of gel filtration chromatography, flotation ultracentifugation and quantitative investigation of ice nucleator activity
using hemolymph from summer and winter collected larvae strongly suggest that highly active protein and lipoprotein ice nucleators
are removed in preparation for overwintering. Additions of either purified antifreeze proteins or midwinter hemolymph with
high antifreeze protein activity to a mixture of protein or lipoprotein ice nucleators isolated from D. canadensis hemolymph inhibited the activity of these nucleators. This suggests that in addition to seasonal removal, inhibition of hemolymph
ice nucleators by antifreeze proteins contributes to seasonal increases in hemolymph supercooling capacity.
Accepted: 8 August 1996 相似文献
5.
Supercooling points, lower lethal temperatures, and the effect of short-term exposures to low temperatures were examined
during both winter and summer in the adults of six weevil species from three different habitats on Marion Island. Upper lethal
limits and the effects of short-term exposure to high temperatures were also examined in summer-acclimatized adult individuals
of these species. Bothrometopus elongatus, B. parvulus, B. randi, Ectemnorhinus marioni, and E. similis were freeze tolerant, but had high lower lethal temperatures (−7 to −10°C). Seasonal variation in these parameters was not
pronounced. Physical conditions of the habitat appeared to have little effect on cold hardiness parameters because the Ectemnorhinus species occur in very wet habitats, whereas the Bothrometopus species inhabit drier areas. The adults of these weevil species are similar to other high southern latitude insects in that
they are freeze tolerant, but with high lower lethal temperatures. In contrast, Palirhoeus eatoni, a supra-littoral species, avoided freezing and had a mean supercooling point of −15.5 ± 0.94°C (SE) in winter and −11.8 ± 0.98°C
in summer. Survival of a constant low temperature of −8°C also increased in this species from 6 h in summer to 27 h in winter.
It is suggested that this strategy may be a consequence of the osmoregulatory requirements imposed on this species by its
supra-littoral habitat. Upper lethal temperatures (31–34°C) corresponded closely with maximum microclimate temperatures in
all of the species. This indicates that the pronounced warming, accompanied by the increased insolation that has been recorded
at Marion Island, may reduce survival of these species. These effects may be compounded as a consequence of predation by feral
house mice on the weevils.
Received: 4 February 1997 / Accepted: 3 May 1997 相似文献
6.
M. Holmstrup L. Sømme 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(3):197-203
Specimens of the Arctic Collembolon Onychiurus arcticus were exposed to desiccation at several subzero temperatures over ice and at 0.5 °C over NaCl solutions. The effects of desiccation
on water content (WC), body fluid melting point (MP), supercooling point (SCP) and survival were studied at several acclimation
temperatures and relative humidities. Exposure to temperatures down to −19.5 °C caused a substantial and increasing dehydration.
At the lowest exposure temperature unfrozen individuals lost 91.6% of the WC at full hydration but more than 80% of the individuals
survived when rehydrated. Exposure at 0.5 °C to decreasing relative humidities (RH) from 100% to 91.3% caused increasing dehydration
and increasing mortality. Survival of equally dehydrated individuals was higher at subzero temperatures than at 0.5 °C. Concurrent
with the decline in WC a lowering of the MP was observed. Animals exposed to −3 °C and −6 °C over ice for 31 days had a MP
of −3.8 and < −7.5 °C, respectively. Specimens from a laboratory culture had a mean SCP of −6.1 °C, and acclimation at 0 or
−3 °C had little effect on SCPs. Exposure at −8.2 °C over ice for 8 days, however, caused the mean SCP to decline to −21.8 °C
due to the severe dehydration of these individuals. Dehydration at 0.5 °C in 95.1 and 93.3% RH also caused a decline in SCPs
to about −18 °C. Individuals that had been acclimated over ice at −12.4 °C or at lower temperatures apparently did not freeze
at all when cooled to −30 °C, probably because all freezeable water had been lost. These results show that O. arcticus will inevitably undergo dehydration when exposed to subzero temperatures in its natural frozen habitat. Consequently, the
MP and SCP of the Collembola are substantially lowered and in this way freezing is avoided. The increased cold hardiness by
dehydration is similar to the protective dehydration mechanism described in earthworm cocoons and Arctic enchytraeids.
Accepted: 5 January 1998 相似文献
7.
D. Audet D. W. Thomas 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(2):146-152
The present study questions whether hypothermia is an artifact due to captivity-induced stress or a thermoregulatory strategy
for bats of the neotropical family Phyllostomidae. In Guanacaste, Costa Rica, Carollia perspicillata and Sturnira lilium exhibited a bimodal distribution of body temperatures when submitted to an ambient temperature of 21 °C. Body temperature
was highly correlated with body mass in both species. C. perspicillata of mass ≥20 g and S. lilium of mass ≥17 g remained normothermic (body temperature >37 °C), whereas at masses below 18 g and 13 g, respectively, >80%
of individuals were hypothermic (body temperature ≤32 °C). In two treatment groups for each species, we restricted food intake
to ca. 20% of body mass on either night 1 or night 4 following capture. Hypothermia was significantly related to food-restriction,
but not time in captivity. Metabolic rate (ml O2 · g−1 h−1) at ambient temperature = 21 °C was MR = e
(–2.11 + 0.101 Tb) (r
2 = 0.7, P < 0.001) for C. perspicillata and MR = e
(−2.62 + 0.115 Tb) (r
2 = 0.89) for S. lilium. Free-ranging, radio tagged C. perspicillata exhibited daily depression of body temperature to 33–34 °C. We conclude that hypothermia is an thermoregulatory strategy
that allows phyllostomid bats to adjust metabolic rate to feeding success and the level of fat stores.
Accepted: 20 August 1996 相似文献
8.
H. Ramløv 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1999,169(3):224-235
The microclimate in the habitat of the New Zealand alpine weta Hemideina maori is very variable with winter temperatures down to −6 °C under the rocks where the insects are found. Subfreezing temperatures
may in winter prevail for up to 17 days but diurnal cycles of freezing and thawing are common, as is also the case in summer.
Rates of temperature change can be very high and up to −7.20 °C/h. During winter, humidity was high for extended periods ranging
from 70% to 100% relative humidity (RH). In the summer, humidity ranged from 30% RH during the day to 100% RH at night. The
supercooling point of the haemolymph was approximately −8 °C year round, caused by a heat labile substance. The supercooling
point of the haemolymph of an insect of the same genus, Hemideina femorata not regularly exposed to subfreezing temperatures, was ca. −16.5 °C. Thermal hysteresis was not detected in the haemolymph
of H. maori. Haemolymph osmolality varied from 380 mOsm (summer) to 700 mOsm (winter). Body water content was ca. 75% all year round.
Total concentrations of sodium, potassium and chloride in haemolymph varied from 170 mM (winter) to 250 mM (summer). The total
concentration of free amino acids varied from 58 mM (summer) to 263 mM (winter). This variation was mostly due to proline
which varied from ca. 15 mM (summer) to ca. 100 mM (winter). The freeze-tolerant weta H. maori is exposed to a highly variable and cold environment all year round and several properties of its haemolymph composition
can be attributed to these climatic conditions, e.g. the presence of ice-nucleating agents and an increase in the concentration
of proline during cold hardening in the autumn.
Accepted: 22 February 1999 相似文献
9.
Weathers WW Gerhart KL Hodum PJ 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2000,170(8):561-572
We measured resting metabolic rates at air temperatures between ca. −5 and 30 °C in snow petrels (Pagodroma nivea), cape petrels (Daption capense), Antarctic petrels (Thalassoica antarctica), and Antarctic fulmars (Fulmarus glacialoides). We measured seven age classes for each species: adults, and nestlings that were 3, 8, 15, 28, 35, and 42 days old. Basal
metabolic rate (BMR) and thermal conductance (C) of adults averaged, respectively, 140% and 100% of values predicted allometrically
for nonpasserine birds. Minimum metabolic rates of unfasted nestlings aged 15–42 days averaged, respectively, 97% and 98%
of predicted adult BMR in Antarctic petrels and snow petrels, versus 119% and 126% of predicted in Antarctic fulmars and cape
petrels. Nestlings of the southerly breeding snow petrel and Antarctic petrel were relatively well insulated compared with
nestlings of other high-latitude seabirds. Adult lower critical temperature (Tlc) was inversely related to body mass and averaged 9 °C lower than predicted allometrically. As nestlings grew, their Tlc decreased with increasing body mass from ca. 14 to 22 °C (depending upon species) at 3 days of age, to −4 to 8 °C when nestlings
attained peak mass. Nestling Tlc subsequently increased as body mass decreased during pre-fledging weight recession. Nestling Tlc was close to mean air temperature from the end of brooding until fledging in the three surface nesting species.
Accepted: 12 July 2000 相似文献
10.
P. Meier J. Zettel 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(4):297-304
In the Swiss Prealps Entomobrya nivalis hibernates in an inactive state, hidden under bark flakes on spruce. For freeze avoidance it relies on thermal hysteresis
proteins (THPs) and polyols (mainly ribitol, with small amounts arabitol and threitol). Polyols are present only during the
inactive state, THPs additionally protect during the transition phase in spring and autumn, when animals are still active
but frosts may occur. Peak values were recorded in February/March for THPs (3.5 °C hysteresis between melting and freezing
point) and for polyols (26 μg mg−1 FW; hemolymph osmolality 680 mosmol l−1). E. nivalis is able to control its hemolymph osmolality independently of body water content. Mean osmolality in summer was 350– 440 mosmol l−1, in winter it was elevated to 650 mosmol l−1, due to a synthesis mainly of ribitol. Body water content varied between 1.8 and 3.3 mg H2O mg−1 DW, depending on humidity conditions. Experiments on triggering of antifreeze synthesis showed the action of temperature
and photoperiod as cues, but there was also evidence for an endogenous rhythm. No clear correlation between antifreeze concentration
and supercooling ability could be established, suggesting that gut content or other parameters also play an inportant role.
Accepted: 18 November 1995 相似文献
11.
Hydromedion sparsutum is a locally abundant herbivorous beetle on the sub-Antarctic island of South Georgia, often living in close association
with the tussock grass Parodiochloa flabellata. Over a 4-day period in mid-summer when the air temperature varied from 0 to 20°C, the temperature in the leaf litter 5–10 cm
deep at the base of tussock plants (the microhabitat of H. sparsutum) was consistently within the range of 5–7.5°C. Experiments were carried out to assess the ability of H. sparsutum larvae collected from this thermally stable environment to acclimate when maintained at lower (0°C) and higher (15°C) temperatures.
The mean supercooling points (freezing temperature) of larvae collected in January and acclimated at 0°C for 3 and 6 weeks
and 15°C for 3 weeks were all within the range of −2.6 to −4.6°C. Larvae in all treatment groups were freeze tolerant. Acclimation
at 0°C significantly increased survival in a 15-min exposure at −8°C (from 27 to 96%) and −10°C (from 0 to 63%) compared with
the field-fresh and 15°C-treated larvae. Similarly, survival of 0°C-acclimated larvae in a 72-h exposure at −6°C increased
from 20 to 83%. Extending the acclimation period at 0°C to 6 weeks did not produce any further increase in cold tolerance.
The concentrations of glucose and trehalose in larval body fluids increased significantly with low temperature acclimation.
Larvae maintained at 15°C for 3 weeks (none survived for 6 weeks) were less able to survive 1-h exposures between 30 and 35°C
than the 0°C-treated samples. Whilst vegetation and snow cover are an effective buffer against low winter temperatures in
many polar insects, the inability of H. sparsutum larvae to acclimate or survive at 15°C suggests that protection against high summer temperatures is equally important for
this species.
Accepted: 2 August 1999 相似文献
12.
Adaptation and acclimation of growth and photosynthesis of five Antarctic red algae to low temperatures 总被引:2,自引:0,他引:2
Temperature requirements for growth, photosynthesis and dark respiration were determined for five Antarctic red algal species.
After acclimation, the stenothermal species Gigartina skottsbergii and Ballia callitricha grew at 0 or up to 5 °C, respectively; the eurythermal species Kallymenia antarctica, Gymnogongrus antarcticus and Phyllophora ahnfeltioides grew up to 10 °C. The temperature optima of photosynthesis were between 10 and 15 °C in the stenothermal species and between
15 and 25 °C in the eurythermal species, irrespective of the growth temperature. This shows that the temperature optima for
photosynthesis are located well below the optima from species of other biogeographical regions, even from the Arctic. Respiratory
rates rose with increasing temperatures. In contrast to photosynthesis, no temperature optimum was evident between 0 and 25 °C.
Partial acclimation of photosynthetic capacity to growth temperature was found in two species. B. callitricha and Gymnogongrus antarcticus acclimate to 0 °C, and 5 and 0 °C, respectively. But acclimation did in no case lead to an overall shift in the temperature
optimum of photosynthesis. B. callitricha and Gymnogongrus antarcticus showed acclimation of respiration to 5 °C, and P. ahnfeltioides to 5 and 10 °C, resulting in a temperature independence of respiration when measured at growth temperature. With respect
to the acclimation potential of the species, no distinction can be made between the stenothermal versus the eurythermal group.
(Net)photosynthetic capacity:respiration (P:R) ratios showed in all species highest values at 0 °C and decreased continuously to values lower than 1.0 at 25 °C. In turn,
the low P:R ratios at higher temperatures are assumed to determine the upper temperature growth limit of the studied species. Estimated
daily carbon balance reached values between 4.1 and 30.7 mg C g−1 FW day−1 at 0 °C, 16:8 h light/dark cycle, 12–40 μmol m−2 s−1.
Received: 4 November 1999 / Accepted: 7 March 2000 相似文献
13.
Borgwardt N Culik BM 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1999,169(2):100-106
Open-flow oxygen and carbon dioxide respirometry was used in Neumünster Zoo (Germany) to examine the energy requirements
of six Asian small-clawed otters (Amblonyx cinerea) at rest and swimming voluntarily under water. Our aim was to compare their energy requirements with those of other warm-blooded
species to elucidate scale effects and to test whether the least aquatic of the three otter species differs markedly from
these and its larger relatives. While at rest on land (16 °C, n = 26), otters (n = 6, mean body mass 3.1 ± 0.4 kg) had a respiratory quotient of 0.77 and a resting metabolic rate of 5.0 ± 0.8 Wkg−1(SD). This increased to 9.1 ± 0.8 Wkg−1 during rest in water (11–15 °C, n = 4) and to 17.6 ± 1.4 Wkg−1 during foraging and feeding activities in a channel (12 °C, n = 5). While swimming under water (n = 620 measurements) in an 11-m long channel, otters preferred a speed range between 0.7 ms−1 and 1.2 ms−1. Transport costs were minimal at 1 ms−1 and amounted to 1.47 ± 0.24 JN−1 m−1 (n = 213). Metabolic rates of small-clawed otters in air were similar to those of larger otter species, and about double those
of terrestrial mammals of comparable size. In water, metabolic rates during rest and swimming were larger than those extrapolated
from larger otter species and submerged swimming homeotherms. This is attributed to high thermoregulatory costs, and high
body drag at low Reynolds numbers.
Accepted: 21 December 1998 相似文献
14.
H. Arabi H. Vandewalle P. Pitor J. de Lattre H. Monod 《European journal of applied physiology and occupational physiology》1997,76(2):122-127
The present experiment was designed to study the importance of strength and muscle mass as factors limiting maximal oxygen
uptake (V˙O2
max
) in wheelchair subjects. Thirteen paraplegic subjects [mean age 29.8 (8.7) years] were studied during continuous incremental
exercises until exhaustion on an arm-cranking ergometer (AC), a wheelchair ergometer (WE) and motor-driven treadmill (TM).
Lean arm volume (LAV) was estimated using an anthropometric method based upon the measurement of various circumferences of
the arm and forearm. Maximal strength (MVF) was measured while pushing on the rim of the wheelchair for three positions of
the hand on the rim (−30°, 0° and +30°). The results indicate that paraplegic subjects reached a similar V˙O2
max
[1.23 (0.34) l · min−1, 1.25 (0.38) l · min−1, 1.22 (0.18) l · min−1 for AC, TM and WE, respectively] and V˙O2
max
/body mass [19.7 (5.2) ml · min−1 · kg−1, 19.5 (6.14) ml · min−1 · kg−1, 19.18 (4.27) ml · min−1 · kg−1 for AC, TM and WE, respectively on the three ergometers. Maximal heart rate f
c
max
during the last minute of AC (173 (17) beats · min−1], TM [168 (14) beats · min−1], and WE [165 (16) beats · min−1], were correlated, but f
c
max
was significantly higher for AC than for TM (P<0.03). There were significant correlations between MVF and LAV (P<0.001) and between the MVF data obtained at different angles of the hand on the rim [311.9 (90.1) N, 313.2 (81.2) N, 257.1
(71) N, at −30°, 0° and +30°, respectively]. There was no correlation between V˙O2
max
and LAV or MVF. The relatively low values of f
c
max
suggest that V˙O2
max
was, at least in part, limited by local aerobic factors instead of central cardiovascular factors. On the other hand, the
lack of a significant correlation between V˙O2
max
and MVF or muscle mass was not in favour of muscle strength being the main factor limiting V˙O2
max
in our subjects.
Accepted: 31 January 1997 相似文献
15.
The effects of temperature on photosynthesis of a rosette plant growing at ground level, Acaena cylindrostachya R. et P., and an herb that grows 20–50 cm above ground level, Senecio formosus H.B.K., were studied along an altitudinal gradient in the Venezuelan Andes. These species were chosen in order to determine
– in the field and in the laboratory – how differences in leaf temperature, determined by plant form and microenvironmental
conditions, affect their photosynthetic capacity. CO2 assimilation rates (A) for both species decreased with increasing altitude. For Acaena leaves at 2900 m, A reached maximum values above 9 μmol m−2 s−1, nearly twice as high as maximum A found at 3550 m (5.2) or at 4200 m (3.9). For Senecio leaves, maximum rates of CO2 uptake were 7.5, 5.8 and 3.6 μmol m−2 s−1 for plants at 2900, 3550 and 4200 m, respectively. Net photosynthesis-leaf temperature relations showed differences in optimum
temperature for photosynthesis (A
o.t.) for both species along the altitudinal gradient. Acaena showed similar A
o.t. for the two lower altitudes, with 19.1°C at 2900 m and 19.6°C at 3550 m, while it increased to 21.7°C at 4200 m. Maximum
A for this species at each altitude was similar, between 5.5 and 6.0 μmol m−2 s−1. For the taller Senecio, A
o.t. was more closely related to air temperatures and decreased from 21.7°C at 2900 m, to 19.7°C at 3550 m and 15.5°C at 4200 m.
In this species, maximum A was lower with increasing altitude (from 6.0 at 2900 m to 3.5 μmol m−2 s−1 at 4200 m). High temperature compensation points for Acaena were similar at the three altitudes, c. 35°C, but varied in Senecio from 37°C at 2900 m, to 39°C at 3550 m and 28°C at 4200 m. Our results show how photosynthetic characteristics change along
the altitudinal gradient for two morphologically contrasting species influenced by soil or air temperatures.
Received: 5 July 1997 / Accepted: 25 October 1997 相似文献
16.
Porto Neto LR Jonsson NN Ingham A Bunch RJ Harrison BE Barendse W;Cooperative Research Centre for Beef Genetic Technologies 《Immunogenetics》2012,64(5):379-388
Ticks and tick-borne diseases have a detrimental impact on livestock production causing estimated losses of around $200 million
per year in Australia alone. Host resistance to ticks is heritable, within-breed heritability estimates being around 0.35,
and with large differences between breeds. Previously a QTL for tick burden was detected on BTA14 at ~72 Mb distal to the
centromere, near the gene receptor-interacting serine-threonine kinase 2 (RIPK2). To identify polymorphisms in this region, we sequenced all exons of the RIPK2 gene, identifying 46 single nucleotide polymorphism (SNP). Using SNP from RIPK2 as well as SNP from the bovine genome sequence, we genotyped two samples, one of 1,122 taurine dairy cattle and one of 761
zebu and zebu composite beef cattle. We confirmed that SNP and haplotypes from this region, including from RIPK2, were associated with tick burden in both dairy and beef cattle. To determine whether RIPK2 influences response to tick salivary gland extract (SGE), an immunisation experiment with tick SGE in a RIPK2 knockout (RIPK2 −/−) mouse strain was conducted. There was a significant (P < 0.05) reduction in IgG production in the RIPK2 −/− mouse in response to the SGE compared to its background strain C57BL/6
as well as the outbred CD1 mouse strain. In addition, antibodies generated by RIPK2 −/− mice recognised a different set of
antigens within SGE when compared to parental-derived antibodies. In summary, the SNP association with tick burden at BTA14
was confirmed and quantitative and qualitative differences in antibody production were observed between RIPK2 −/− and wild-type
mice. 相似文献
17.
Baudinette RV Churchill SK Christian KA Nelson JE Hudson PJ 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2000,170(5-6):439-446
The ghost bat, Macroderma gigas, and the orange leaf-nosed bat, Rhinonycteris aurantius, occupy similar ranges across northern Australia and are often found in the same roost caves. Both species are considered
rare and vulnerable to further population decline. A third small species, the large bent-wing bat, Miniopterus schreibersii, has a similar body mass to R. aurantius, but has one of the largest ranges of any Australian mammal. In the present study we examine the effect and sensitivity of
the animals' roosting microclimates on their energy and water balance. M. schreibersii exhibits a basal metabolic rate about 40% greater than other bats of similar body mass, whereas the other two species are
close to predicted levels. R. aurantius shows a decrease in body temperatures below thermoneutrality. R. aurantius has levels of pulmocutaneous water loss among the highest seen for a mammal, and calculations based on nasal tip temperatures
suggest that most of this loss is across the skin. Calculated ambient temperatures at which metabolic water production is
equal to pulmocutaneous water loss in dry air are −14.7 °C for R. aurantius, 9.8 °C for M. schreibersii and −0.3 °C for M. gigas. Exposing the animals to relative humidities of between 80% and 90% shifted these calculated temperatures to 5.6 °C, 25.2 °C,
and 2.9 °C, respectively. For each species the ratio of metabolic water production to evaporative water loss has been treated
as a joint function of humidity and ambient temperature. The resulting surface plot shows that under known roosting conditions
in caves R. aurantius and M. schreibersii remain in positive water balance, whereas M. gigas does not.
Accepted: 20 May 2000 相似文献
18.
Cryoprotective and osmotic responses to cold acclimation and freezing in freeze-tolerant and freeze-intolerant earthworms 总被引:3,自引:3,他引:0
M. Holmstrup J. P. Costanzo R. E. Lee Jr 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1999,169(3):207-214
In this paper we present the results of physiological responses to winter acclimation and tissue freezing in a freeze-tolerant
Siberian earthworm, Eisenia nordenskioeldi, and two freeze-intolerant, temperate earthworm species, Lumbricus rubellus and Aporrectodea caliginosa. By analysing the physiological responses to freezing of both types we sought to identify some key factors promoting freeze
tolerance in earthworms. Winter acclimation was followed by a significant increase in osmolality of body fluids in E. nordenskioeldi, from 197 mosmol kg−1 in 10 °C-acclimated animals to 365 mosmol kg−1 in animals acclimated to 0 °C. Cold acclimation did not cause any change in body fluid osmolality in the two freeze-intolerant
species. As a response to ice formation in the body, the freeze-intolerant species produced copious amounts of slime and expulsion
of coelomic fluids, and thereby lost 10–30% of their total water content. Contrary to this, the freeze-tolerant species did
not lose water upon freezing. At temperatures down to −6.5 °C, the ice content in the freeze-tolerant E. nordenskioeldi was significantly lower than in L. rubellus. At lower temperatures there were no differences in ice content between the two species. Cold acclimated, but unfrozen, specimens
of all three species had low levels of ammonia, urea, lactate, glycerol and glucose. As a response to ice formation, glucose
levels significantly increased within the first 24 h of freezing. This was most pronounced in E. nordenskioeldi where a 153-fold increase of glucose was seen (94 mmol · l−1). In L. rubellus and A. caliginosa a 19-fold and 17-fold increase in glucose was seen. This is the first study on physiological mechanisms promoting freeze
tolerance in E. nordenskioeldi, or any other oligochaete. Our results suggest that the cryoprotective system of this species more closely resembles that
of freeze-tolerant anurans, which synthesize cryoprotectants only after tissues begin to freeze, than that of cold-hardy invertebrates
which exhibit a preparatory accumulation of cryoprotectants during seasonal exposure to low temperature.
Accepted: 10 February 1999 相似文献
19.
Booth DT Thompson MB Herring S 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2000,170(4):269-276
Eggs of two small Australian lizards, Lampropholis guichenoti and Bassiana duperreyi, were incubated to hatching at 25 °C and 30 °C. Incubation periods were significantly longer at 25 °C in both species, and
temperature had a greater effect on the incubation period of B. duperreyi (41.0 days at 25 °C; 23.1 days at 30 °C) than L. guichenoti (40.1 days at 25 °C; 27.7 days at 30 °C). Patterns of oxygen consumption were similar in both species at both temperatures,
being sigmoidal in shape with a fall in the rate of oxygen consumption just prior to hatching. The higher incubation temperature
resulted in higher peak and higher pre-hatch rates of oxygen consumption in both species. Total amount of oxygen consumed
during incubation was independent of temperature in B. duperreyi, in which approximately 50 ml oxygen was consumed at both temperatures, but eggs of L. guichenoti incubated at 30 °C consumed significantly more (32.6 ml) than eggs incubated at 25 °C (28.5 ml). Hatchling mass was unaffected
by either incubation temperature or the amount of water absorbed by eggs during incubation in both species. The energetic
production cost of hatchling B. duperreyi (3.52 kJ · g−1) was independent of incubation temperature, whereas in L. guichenoti the production cost was greater at 30 °C (4.00 kJ · g−1) than at 25 °C (3.47 kJ · g−1). Snout-vent lengths and mass of hatchlings were unaffected by incubation temperature in both species, but hatchling B. duperreyi incubated at 30 °C had longer tails (29.3 mm) than those from eggs incubated at 25 °C (26.2 mm). These results indicate that
incubation temperature can affect the quality of hatchling lizards in terms of embryonic energy consumption and hatchling
morphology.
Accepted: 27 January 2000 相似文献
20.
F O Ayorinde K A Saeed E Price A Morrow W E Collins F McInnis S K Pollack B E Eribo 《Journal of industrial microbiology & biotechnology》1998,21(1-2):46-50
Saponified vernonia oil was converted exclusively to poly(β-hydroxybutyrate) (PHB) by Alcaligenes eutrophus in a single-stage batch culture. After harvesting, centrifugation followed by lyophilization, the resulting dried cells contained
up to 42.8 wt% PHB having a peak molecular mass of 381 863 Da, weight-average molecular mass of 308 390 Da, and a polydispersity
of 1.1. The PHB had a melting point (Tm) range of 163–174°C with a maximum at 172°C (lit. Tm, 175°C), and heat of fusion of 18.43 cal g−1. Fermentation performed under varying conditions of nitrogen limitation indicated that there was no significant effect of
nitrogen concentration on the molecular mass of PHB produced from vernonia oil by A. eutrophus.
Received 27 March 1998/ Accepted in revised form 17 July 1998 相似文献