Visual response of the tomato fruit fly, Neoceratitis cyanescens, to colored fruit models

To determine how mature females of the tomato fruit fly, Neoceratitis cyanescens (Bezzi) (Diptera: Tephritidae), detect host fruit after arriving in their host plant habitat, behavioral responses to colored models were observed in a laboratory flight chamber. Host‐seeking females oriented themselves preferentially towards bright orange spheres (3.7 cm in diameter), irrespective of their natal host fruit: tomato, bug weed, or black nightshade. Females oriented themselves preferentially towards the orange sphere when placed against a fluorescent yellow background as opposed to a black background, but the distribution of responses to the set of colored spheres did not vary significantly with background color. In a choice situation between bright orange spheres of various sizes (1.9, 3.7, and 7.5 cm in diameter), females landed preferentially on the bigger sphere. However, they preferred a yellow color when the latter was associated with two‐dimensional models, probably mimicking leaves. The attractiveness of orange spheres depended more on the proportion of reflected light in the spectral region around 610 nm than brightness of color in itself. Low light intensity significantly influenced the activity of the flies but not their visual preference. The strong response of females to bright orange spheres confirmed the importance of visual characteristics in short‐range mechanisms of host‐plant location in specialist insects. Responses to fruit visual stimuli are discussed relative to other Tephritidae, host‐finding strategy, and pest management.     

Oviposition preference in the oligophagous tomato fruit fly, Neoceratitis cyanescens

We assessed the role of visual and olfactory cues on oviposition preference in the oligophagous tomato fruit fly, Neoceratitis cyanescens (Bezzi) (Diptera: Tephritidae). In a field survey, we evaluated the stage of susceptibility of field‐grown tomatoes by monitoring N. cyanescens infestations from fruit‐setting up to harvest, in relation to post‐flowering time, size, and visual properties of fruit. In two‐choice laboratory experiments, we tested the degree to which females use visual and olfactory cues to select their host plant for oviposition. In addition, we investigated the ability of flies to avoid fruit already infested by conspecific eggs or larvae, and the influence of natal host fruit on oviposition preference. Neoceratitis cyanescens females preferentially lay their eggs in small yellow‐green unripe fruit (2–3.5 cm diameter, 10–21 days post‐flowering). Damage to fruit was significantly affected by brightness and size properties. In laboratory experiments, females chose to lay their eggs in bright orange rather than yellow domes. On the sole basis of olfactory stimuli, females showed a significant preference for unripe vs. ripe host fruit, for unripe fruit vs. flowers or leaves, and for host vs. non‐host fruit (or control). However, colour interacted with odour as females dispatched their eggs equally between the yellow dome and the bright orange dome when unripe fruit of tomato was placed under the yellow dome vs. ripe fruit under the bright orange dome. When offered real ripe and unripe tomatoes, females preferred unripe tomatoes. Females significantly chose to lay eggs in non‐infested fruit when they were given the choice between these or fruit infested with larvae. In contrast, recent stings containing eggs did not deter females from laying eggs. Rather, they could have an attractive effect when deposited within <1 h. Regardless of their natal host plant, tomato or bugweed, N. cyanescens females laid significantly more eggs in a dome containing bugweed fruit. However, 15% of females originating from tomato laid eggs exclusively in the dome with tomato, against 3% of females originating from bugweed.     

Physical and chemical factors influencing landing and oviposition by the cabbage root fly on host-plant models

Various plant models were used in both choice and no-choice tests in the laboratory to assess landing and oviposition preferences of the cabbage root fly,Delia radicum (L.). The main factor governing the site most suitable for landing was the conspicuousness of the object and not its shape. Oviposition was influenced considerably by the pre-conditioning of the females. Deprived females laid eggs even when denied access to both host plant chemicals and host-plant models. The dominant role of contact chemical stimuli in host acceptance was reconfirmed, but only a combination of physical and chemical stimuli appeared capable of eliciting normal oviposition. The combination of contact chemical stimuli and the presence of a stem on the test model had a synergistic effect on the numbers of eggs laid in both choice and no-choice situations. In choice bioassays, female cabbage root flies distinguished between models of different shapes, heights and sizes. The size and shape of the models appeared to be perceived in part after the flies had landed.     

The effect of interspecific mating on sex ratios in the twospotted spider mite and the Banks grass mite (Acarina: Tetranychidae)

Mixed populations of the twospotted spider mite (TSM),Tetranychus urticae (Koch), and the Banks grass mite (BGM),Oligonychus pratensis (Banks), occur on corn and sorghum plants in late summer in the Great Plains. Interspecific matings between these arrhenotokous species occur readily in the laboratory but yield no female offspring. The effect of interspecific mating on female: male sex ratios was measured by examining the F₁ progeny of females that mated with both heterospecific and conspecific males in no-choice situations. TSM females that mated first with BGM males and then with TSM males produced a smaller percentage of female offspring than TSM females that mated only with TSM males (43.1±5.8 and 78.9±2.8% females, respectively). Similarly, BGM females mated with heterospecific males and then with conspecific males produced fewer female offspring than females mated only with BGM males (55.7±5.2 and 77.5±2.5%, respectively). Lower female: male sex ratios were produced also by BGM females that mated with TSM males after first mating with conspecifics (62.4±3.4%). In mixed populations containing males of both species, females also produced lower female: male sex ratios, but these ratios were not as low as expected based on mating propensities and progeny sex ratios observed in no-choice tests. These data suggest that interspecific mating may substantially reduce female fitness in both mite species by reducing the output of female offspring, but in mixed populations this effect is mitigated by unidentified behavioral mechanisms.     

Effect of physiological status on olfactory and visual responses of female Hypothenemus hampei during host plant colonization

The response of Hypothenemus hampei (Ferr.) (Coleoptera, Scolytidae) (coffee berry borer, CBB) females to olfactory stimuli was demonstrated to be related with the physiological status; especially with age, whether they were mated or not, and whether they have already oviposited. A special behaviour, the abandonment of the native berry, was also found to play a major role. In contrast, response to visual stimuli (false coffee berries) was found to be independent of the physiological status of CBB females. Variations in behavioural responses to visual and olfactory stimuli are discussed in relation to colonization strategy.     

Preference of the cabbage root fly, Delia radicum (L.), for coloured traps: influence of sex and physiological status of the flies, trap background and experimental design

Abstract. Catches of Delia rudicum (L.) (Diptera: Anthomyiidae) were compared in water traps that reflected predominantly wavelengths shorter (violet and blue traps) and longer (green and yellow traps) than 500 nm.Traps were positioned in choice and no-choice situations against backgrounds of bare soil and weeds in the field and against backgrounds of brown and green paper in the laboratory.The physiological status of the flies was modified in the laboratory by denying them access to food sources and oviposition sites.
Males discriminated significantly more clearly than females between yellow and blue traps.The discrimination between yellow and blue traps was significantly more pronounced when the traps were presented in the choice than in the no-choice situation in both sexes.Green background (weeds and green paper) was highly preferred for landing and thus competed with the traps to such an extent that few flies were caught when non-preferred violet and blue traps were sited on green backgrounds.Flies seldom landed on the brown background (soil and brown paper) which resulted in the relative increase of catches in the non-preferred violet and blue traps.The preference for yellow traps was innate even in young flies with immature egg-follicles.Females that were ready to lay eggs, even those deprived of an oviposition site till the age of 8 days, also preferred yellow traps.In the no-choice situation, flies deprived of food landed with the same frequency in yellow and blue traps.Food deprivation, however, did not affect preference for yellow traps over the blue traps presented in a choice situation.     

Sexual Attraction,Male Courtship and Female Remating in the Tomato Fruit Fly, <Emphasis Type="Italic">Neoceratitis cyanescens</Emphasis>

Sexual attraction in the tomato fruit fly, Neoceratitis cyanescens (Bezzi) (Diptera: Tephritidae) was tested in an olfactometer while major behavioral traits during courtship at a short range were described by video recording. In addition, 30 pairs of flies were monitored for mating activity in individual cages, during a 38-day study following adult emergence. Virgin females of N. cyanescens showed a significant attraction when males were placed in the upwind section of the olfactometer. Males adopted a typical calling behavior characterized by immobility, wings perpendicular to the body axis, swollen abdominal pleura and presence of a small transparent droplet at the tip of the abdomen. In addition, a specific odor was clearly perceptible by the observer. In individual cages, the first mating of N. cyanescens females took place on the second day after emergence. Females showed a high mating propensity, with 73 ± 18% females mated daily. Sexual conflicts due to mating system and applications to pest management strategies are discussed.     

Cross-mating tests and the species status of Trissolcus basalis reared from the eggs of differentheteropteran species

Laboratory choice and no-choice cross-mating tests were conducted to establish whether Trissolcus basalis individuals from green vegetable bug, Nezara viridula, and nine other host species sharemating characteristics and belong to the same species. In no-choice cross-mating tests, all individuals in self- and out-crosses mated within 10 minutes. Although all females mated, they were not all inseminated successfully. However, a similar proportion of females was inseminated across all self- and out-crosses in each set of cross-mating tests. Mate-choice tests indicated that males and females selected mates at random. Overall, the results indicate that T. basalis individuals from the ten host species represent a single genetical species. This revised version was published online in July 2006 with corrections to the Cover Date.     

Flower and fruit volatiles assist host‐plant location in the Tomato fruit fly Neoceratitis cyanescens

Mature females of the tomato fruit fly Neoceratitis cyanescens can detect host fruit at a short distance using only visual stimuli, but little is known about the role of airborne volatile cues in the host searching strategy. A series of experiments is conducted in a laboratory wind tunnel, in which the behavioural responses of individual flies to volatiles from Solanaceae host plants (including tomato Lycopersicum esculentum Mill., bug weed Solanum mauritianum Scop. and Turkey berry Solanum torvum Sw.) are observed, according to some environmental (air speed) and physiological (age and mating status of females, time of day) factors. Mature females respond primarily to specific olfactory cues from blends of flowers or host fruit, preferentially unripe fruit for bug weed, as opposed to ripe fruit for Turkey berry or tomato. Males are also highly attracted by the odour of unripe fruit of bug weed. Wind plays a key role, as shown by the proportion of flies that reach the upwind section of the tunnel in the presence of both fruit odour and air flow (66.7%) and in the absence of either fruit odour (13.3%) or wind (36.7%). In response to fruit volatiles carried by wind, flies embark in a ‘plume tracking’ or ‘aim and shoot' flight, consistent with odour‐conditioned anemotaxis. Females respond to host fruit odour regardless of their age, egg load or mating status, and also more consistently in the afternoon, which is their preferential time of day for egg‐laying. Searching behaviour and response to host volatiles in N. cyanescens are discussed in the light of host‐finding and an adaptive strategy.     

Pea plant volatiles guide host location behaviour in the pea moth

Identification of plant volatiles that attract mated insect females for oviposition can provide important information about plant–insect relationships that can be used to develop pest control strategies involving manipulation of the female host search. Our study represents a first step towards identifying volatiles that affect the host location behaviour of the pea moth Cydia nigricana. The behaviours of virgin and mated males and females were analysed in cage experiments testing a two-choice situation at close range and in wind tunnel experiments evaluating upwind orientation over a distance. In both experimental setups, flowering pea plants constituted the most attractive phenological stage for mated females, with 58 % landing on such plants in the wind tunnel. Testing headspace extracts of different phenological stages of pea and of detached pea buds and flowers in the wind tunnel, mated females showed the highest landing responses to volatiles during flower development (budding 42 % and flowering 56 %) and from detached buds (46 %) and flowers (66 %). Volatile compounds collected from the various phenological stages of pea were analysed by gas chromatography–mass spectrometry, and the antennal responses to these headspace collections were evaluated by gas chromatography–electroantennography. Ten antennally active compounds were identified, nine of which were present in the headspace extracts of the whole pea plants at all tested phenological stages and in detached buds and flowers. Overall, our results demonstrate a clear link between host plant phenology, the corresponding plant odour, and the behaviour of mated C. nigricana females.     

Olfactory response of Anastrepha striata (Diptera: Tephritidae) to guava and sweet orange volatiles

The behavioral responses of virgin and mated female Anastrepha striata Schiner (Diptera: Tephritidae) to guava (Psidium guajava L.) or sweet orange (Citrus sinensis L.) were evaluated separately using multilure traps in two‐choice tests in field cages. The results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). The physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. Combined analysis of gas chromatography coupled with electroantennography (GC‐EAD) of volatile extracts of both fruits showed that 1 and 6 compounds from orange and guava, respectively elicited repeatable antennal responses from mated females. The EAD active compounds in guava volatile extracts were identified by gas chromatography‐mass spectrometry (GC‐MS) as ethyl butyrate, (Z)‐3‐hexenol, hexanol, ethyl hexanoate, hexyl acetate, and ethyl octanoate. Linalool was identified as the only antennal active compound in sweet orange extracts. In field cage tests, there were no significant differences between the number of mated flies captured by the traps baited with guava extracts and the number caught by traps baited with the 6‐component blend that was formulated according to the relative proportions in the guava extracts. Similar results occurred when synthetic linalool was evaluated against orange extracts. From a practical point of view, the compounds identified in this study could be used for monitoring A. striata populations.     

Orange-derived stimuli regulating oviposition in the Mediterranean fruit fly

Abstract:  Some visual and olfactory host stimuli influencing oviposition of the Mediterranean fruit fly ( Ceratitis capitata Wiedemann) in orange fruit were investigated in a laboratory-reared strain of this species. Mated females of C. capitata were found to be attracted to the same extent by fragrant orange fruits and odourless sham oranges, while unmated females were notably less attracted than mated females by the above objects. Mated females laid significantly more eggs in orange-coloured than in whitish (optically neutral) spherical wax dummies (diameter, 9.0 cm) as well as in both orange-coloured and whitish wax dummies supplemented with high internal humidity (80–100%) compared with respective wax dummies supplied with low internal humidity (30–50%). The orange-like colour together with high internal humidity provides basic stimuli supporting adequate oviposition of C. capitata in oranges. The oviposition rate of female C. capitata was not significantly changed when graded dosages ranging from 0.004 to 19.6  μ l of orange peel oil per cm² were added to orange-coloured wax dummies, while oviposition was considerably subdued by addition of 3.9  μ l orange peel oil and completely disrupted by addition of 9.8 or 19.6  μ l of orange peel oil per cm² of whitish wax dummies. Oviposition of C. capitata in ripe orange fruit may thus be interpreted by the predilection of this tephritid species for an orange-coloured, glossy pericarp, being capable of counteracting the deterrent effect of the essential oil found in orange flavedo.     

Aggressive Behavior and Courtship Rejection in Brightly and Plainly Colored Female Keeled Earless Lizards (Holbrookia propinqua)

Relationships between bright secondary sexual coloration and behavior were studied in female Holbrookia propinqua, which develop striking orange and yellow colors during the breeding season. In tethered introduction studies, brightly colored females performed aggressive courtship rejection behaviors toward conspecific males; plainly colored females were not aggressive toward males, but attempted to avoid them. Responses of females of the two color patterns toward conspecific females of both color phases were not detectably different. Experimental introductions of lizards with coloration modified by paint showed that females of both color patterns recognize any other lizard bearing the bright female colors as female, regardless of actual sex. Both the orange and yellow components were shown to contribute to sex recognition. The yellow component alone allowed accurate sex identification, but only half the females responded to males painted with only the orange female component as if they were females. Because females did not behave differently toward other females on the basis of coloration, the hypothesis that bright coloration evolved as an adaptive signal between females is rejected. The dark ventrolateral stripes of male and plainly colored females did not appear to affect intraspecific social responses by females.     

Calling behavior of the female german cockroach,Blattella germanica (Dictyoptera: Blattellidae)

Virgin German cockroach females, Blattella germanica(L.), were observed, for the first time, to exhibit a characteristic calling behavior during which females emit a volatile sex pheromone. Under a photoperiod of 12L12D, the percentage of 7-day-old virgin females that exhibited this behavior peaked before the end of the scotophase in a similar pattern to the diel periodicity of mating. A clear relationship was evident between calling and stages of sexual receptivity during successive gonotrophic cycles. Females initiated calling 5–6 days after the imaginal molt, when their basal oocytes were 1.6 mm long. If not mated, females continued to exhibit bouts of calling during the next 3–4 days until 24 h before ovulation. Calling was completely suppressed by mating as well as the presence of an egg case in the genital atrium in both virgin and mated gravid females. We suggest that calling and the emission of a volatile sex pheromone serve to attract males from a distance as well as to potentiate responses to contact sex pheromone in aggregations.     

Host location by chemical stimuli in Bracon hylobii (Ratzeburg) (Hymenoptera: Braconidae), a larval parasitoid of Hylobius abietis (L.) (Coleoptera: Curculionidae)

The Large Pine Weevil, Hylobius abietis, is one of the most important pests in European coniferous forests. The ectoparasitoid Bracon hylobii is known to cause around 50% larval mortality. Chemical volatiles are among the most important stimuli involved in the host location. In the present study, a 4-arm olfactometer and a 2-choice wind-tunnel were used to test the behavioural responses of B. hylobii females to four odour sources from the host-plant/host-insect complex. For each odour, the behaviour of twenty virgin females was individually recorded in an olfactometer, whereas twenty virgin and twenty mated females were individually tested in a wind-tunnel. The following behavioural parameters were analysed: a) time spent walking; b) time spent stopped; c) total time spent in each arm; d) total speed; e) net speed; f) the rate of turning per unit distance walked; g) rate of turning per second. Comparison between odours shows clear differences in the behavioural parameters analysed. In particular, the bark disc/larva complex is the only odour source able to elicit behavioural responses of both naive and mated females. As strong responses were obtained only using experienced females, this suggests that previous searching activities increase the ability of B. hylobii to find its host.     

Vision and electroreception: Integration of sensory information in the optic tectum of the weakly electric fishApteronotus albifrons

Summary The responses of single neurons to visual and electrosensory stimulation were studied in the optic tectum of the weakly electric fishApteronotus albifrons. Most of the cells recorded in the region of the tectum studied, the anterior medial quadrant, were poorly responsive or completely insensitive to flashes of light or to bursts of AC electrical stimuli applied to the entire fish. However, these cells gave vigorous responses to moving visual or electrosensory stimuli. Most cells showed differences in their response contingent upon the direction of the stimulus movement and most received input from both the visual and electrosensory systems. Electrosensory responses to moving stimuli were depressed by jamming stimuli, 4 Hz amplitude modulation of the animal's electric organ discharge, presented simultaneously with the moving stimulus. However, the jamming signal presented alone typically evoked no response. Moving visual stimuli, presented simultaneously with the electrosensory, were usually able to restore the magnitude of a response toward its value in the unjammed situation. For most of the cells studied the receptive fields for vision and electroreception were in register. In some cases the visual and electrosensory components could be separated by presenting the two types of stimuli separately, or by presenting both simultaneously but with some amount of spatial separation, which causes the two to be misaligned relative to the fish. In other cases the individual responses could not be separated by spatial manipulations of the two stimuli and in these cases differences in the alignment of the two types of stimuli could cause changes in the intensity of the cells' responses.Abbreviations AM amplitude modulation - EOD electric organ discharge - PLLL posterior lateral line lobe     

Male mate choice and the potential for complex mating dynamics in the tree lizard (Urosaurus ornatus)

A growing body of literature is recognizing that males may also play a role in the mating process by behaving non‐randomly toward potential female mates during courtship. In numerous species, discrete color polymorphisms in males are inferred to represent alternative mating tactics, which often correspond with concomitant asymmetries in ecology and behavior. In terms of their mating behavior, these ecological outcomes of a color polymorphism should affect a morph's likelihood and frequency of encountering females in a population, possibly favoring the evolution of morph‐specific mating preferences. Knowledge of how male morphs contribute to a species’ overall mating dynamics will improve our understanding of how sexual selection shapes phenotypic diversity in color polymorphic systems. We conducted a mate choice experiment to evaluate the extent and morph specificity of non‐random mating preferences by male ornate tree lizards, Urosaurus ornatus. We observed the behavior of blue and yellow males in an experimental arena in response to a choice between an orange or yellow female. We found that blue males preferred yellow females over orange females, and although yellow males visited females more often than blue males overall, their attention was not morph‐specific. Given male morph differences in choosiness, and their differences in social dominance, we conclude that female throat color may be partly under sexual selection in U. ornatus. However, a lack of concordance between male and female mating preferences (drawn from an earlier study) suggests that overall mating dynamics may serve to maintain, rather than enhance, color morph differences in this species.     

Visual responses of apple maggot flies, Rhagoletis pomonella (Diptera: Tephritidae): Orchard studies

Apple maggot flies, Rhagoletis pomonella, were found to be highly responsive to visual stimuli, with maximum responses dependent upon color, shape, and size characteristics of the stimulus. Each sex had a significant preference for yellow 30×40 cm rectangles over green, orange, red, blue, violet, black, white and clear rectangles of the same size. On the other hand, each sex had a significant preference for red, blue, violet, dark organge, and black spheres 7.5 cm in diameter over green, light orange, yellow, white, and clear spheres of the same size. Both sexes had a significant preference for 7.5 cm spheres over equivalent-sized cubes, cylinders and rectangles. As the diameter of a sphere was increased from 7.5 to 45 cm, there was an orderly and significant decrease in the attractiveness of those that were darkcolored (red) but a progressive and significant increase in the attractiveness of those that were yellow. The flies did not congregate on trees with apples or respond strongly to 7.5 cm, darkcolored spheres until they were sexually mature (at least in the case of females). Relative to other colors, females were often significantly more attracted than males to yellow. The attractiveness of an olfactory stimulus eliciting feeding-type reactions was enhanced to a substantial degree when employed in conjunction with a 30×40 cm yellow rectangle, but to only a very slight degree when in conjunction with a 7.5 cm red sphere.Evidence presented suggests the following explanation for these findings. The flies are attracted to large surfaces of yellow because they react to yellow, on the basis of true color discrimination, as if it were foliage on which to find food. On the other hand, they are attracted to small, dark-colored spheres because they react to such spheres as they react to apples, which are the oviposition site and which also appear to serve as a rendezvous site for the sexes in mating activity. Once the flies have arrived on apple trees, they detect apples solely through vision. Red, blue, violet, dark orange, and black small spheres are preferred over small spheres of other colors on the basis that they stand out in strongest contrast against the background and not on the basis of true color discrimination. Large, dark-colored spheres are unattractive because the range of sphere sizes eliciting positive responses is near the size of an apple.These findings have proven useful in designing effective devices for sampling orchard populations of the flies and may also prove useful in fly control.

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Zusammenfassung Imagines von Rhagoletis pomonella reagieren positiv auf visuelle Reize, wobei Farbe, Form und Grösse des Stimulus von ausschlaggebender Bedeutung sind. Beide Geschlechter zeigten in den Versuchen eine signifikante Präferenz für gelbe Rechtecke (30×40 cm), die sie häufiger anflogen als grüne, orange, rote, blaue, violette, schwarze, weisse und farblose Flächen gleicher Grösse. Ebenso zeigte es sich, dass Kugeln von 7.5 cm Durchmesser von den Fliegen eindeutig Kuben, Zylindern und Rechtecken gleicher Grössenordnung für den anflug bevorzugt wurden. Mit zunehmendem Durchmesser der Kugeln von 7.5 cm bis 45 cm konnte eine abnehmende Anziehung von dunkel gefärbten Kugeln, jedoch eine zunehmende Anziehung von gelben Kugeln auf die Fliegen beobachtet werden. Vor der Geschlechtsreife reagierten die Weibchen nicht in ausgeprägtem Maße auf die 7.5 cm dunkel gefärbte grossen Kugeln, sondern wurden mehr als die Männchen von Gelb angezogen. Die Wirkung eines Nahrungsköders wurde in Kombination mit einer gelben Fläche von 30×40 cm signifikant erhöht, während die Kombination des Köders mit einer roten Kugel von 7.5 cm Durchmesser die Anziehung von Weibchen nur unwesentlich erhöhte.Die Resultate lassen sich damit erklären, dass die Fliegen durch gelbe Flächen angezogen werden, weil sie dank Diskriminierung der Farbe auf Gelb reagieren als wäre es Blattwerk, auf dem sich die Nahrungsquellen befinden. Auf der anderen Seite werden sie von dunklen Kugeln in den Dimensionen eines Apfels angezogen, da im Freiland Äpfel Orte für die Eiablage und offenbar Treffpunkte für die Geschlechter darstellen. Sobald die Fliegen den Apfelbaum beflogen haben, orientieren sie sich allein mit dem Gesichtssinn gegen die Früchte. Kleinere rote, blaue, dunkel orange, violette und schwarze Kugeln werden anders gefärbten vorgezogen, weil sie den grössten Kontrast gegenüber dem Hintergrund bilden und nicht auf Grund echter Farbdiskriminierung. Grosse dunkle Kugeln verlieren ihre Wirkung auf die Fliegen, weil nur Kugeln in der Grössenordnung eines Apfels positive Reaktionen auslösen.Diese Befunde haben sich als nützlich erwiesen in der Ausarbeitung von Sammelmethoden für Freilandpopulationen und dürften auch in der Bekämpfung der Apfelfliegen Anwendung finden.

     

Habitat cues synergize to elicit chemically mediated landing behavior in a specialist phytophagous insect,the grape berry moth

Many phytophagous insects locate their host plant using mixtures of volatile compounds produced by the plant. A key behavior in the host location process that has been the focus of decades of behavioral research is optomotor anemotaxis. Another key step in host location is landing on (or near) the odor source. In previous work, rubber septa emitting a synthetic blend of volatiles extracted from young shoots of grape plants, Vitus spp. (Vitaceae), elicited equivalent levels of oriented upwind flight by female grape berry moths (GBM), Paralobesia viteana (Clemens) (Lepidoptera: Tortricidae), as did actual (control) grape shoots. However, in contrast to the shoots, females did not land on the odor source. In this study, we used flight tunnel assays to investigate the landing response of GBM females with respect to chemical and visual stimuli, as well as differences in relative humidity. When stimuli were presented individually, only the synthetic blend of host plant volatiles elicited equivalent levels of oriented upwind flight compared to the plants. Interestingly, wet cotton strips elicited low but consistent levels of upwind flight. In paired assays, only the synthetic blend paired with wet cotton strips elicited landing, although at significantly lower levels than that elicited by grape shoots. To achieve landing rates equivalent to live grape shoots, grape berry moth females required all three stimuli we tested: host odor cues, moisture, and visual cues simulating a grape shoot. These results suggest the cues have a synergistic effect, and that landing behavior requires complex sensory processing using multiple sensory inputs. Furthermore, these results suggest that moisture plays an important role in the host plant location process.     

Attraction of fruit flies of the genus Bactrocera to colored mimics of host fruit

In tests on feral populations of polyphagous Bactrocera tryoni (Froggatt) adults on host guava trees, both sexes were significantly more attracted to Tangletrap‐coated 50 mm diameter spheres colored blue or white than to similar spheres colored red, orange, yellow, green, or black or to Tangletrap‐coated 50 mm diameter yellow‐green guava fruit. In contrast, in tests on feral populations of oligophagous Bactrocera cacuminata (Hering) on host wild tobacco plants, both sexes were significantly more attracted to Tangletrap‐coated 15 mm diameter spheres colored orange or yellow than to other colors of spheres or to Tangletrap‐coated 15 mm diameter green wild tobacco fruit. Both sexes of both tephritid species were significantly more attracted to blue (in the case of B. tryoni) or orange (in the case of B. cacuminata) 50 mm spheres displayed singly than to blue or orange 15 mm spheres displayed in clusters, even though fruit of wild tobacco plants are borne in clusters. Finally, B. tryoni adults were significantly less attracted to non‐ultraviolet reflecting bluish fruit‐mimicking spheres than to bluish fruit‐mimicking spheres having a slightly enhanced level of ultraviolet reflectance, similar to the reflectance of possible native host fruit of B. tryoni, whose bluish skin color is overlayed with ultraviolet‐reflecting waxy bloom. Responses to fruit visual stimuli found here are discussed relative to responses found in other tephritid species.