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1.
A fungus causing zonate leaf blight diseases in various evergreen and deciduous woody plant species in Japan was characterized by a discoid multicellular propagule arising from a hyaline sclerotium-like structure in the leaf tissue and dark-coloured microconidia produced enteroblastically from the terminal cells on the surface of the discoid propagules. Myrioconium-like microconidiophores also producing microconidia were occasionally produced in culture. No teleomorphic characteristics were observed on the fungus. Molecular analysis based on the partial nu-rDNA sequence data revealed that the fungus was phylogenetically related to the Sclerotiniaceae, Leotiomycetes, and Ascomycota. Because the morphology and sequence data of this fungus does not coincide with those of any known anamorphic fungi, Haradamyces foliicola is proposed here as a new anamorphic genus and species for this fungus. 相似文献
2.
Michael M?ller Alan Forrest Yi-Gang Wei Anton Weber 《Plant Systematics and Evolution》2011,292(3-4):223-248
Based on a considerably enlarged sampling, a phylogenetic analysis of the largest group of didymocarpoid Gesneriaceae, the ??advanced Asiatic and Malesian genera??, was performed, covering all but 3 of the 60 genera presently recognised in this group (20 of these, mostly from China, are monotypic). The results suggest that no fewer than 17 out of the 57 genera examined are poly- (or rarely para-)phyletic. Highly polyphyletic are Briggsia, Chirita, Henckelia and Raphiocarpus. Only a dozen of the non-monotypic genera (including the three species-richest genera, Cyrtandra, Aeschynanthus and Agalmyla) are confirmed as monophyletic entities, though some exhibit considerable genetic variation. For eight genera, no statement can be made, as only one (of two or several) species was included in the analysis. For a dozen of the (particularly Chinese) monotypic genera a close relationship (or possible congenerity) with other genera was found. In China, only Allostigma, Cathayanthe, Conandron and Metapetrocosmea seem to have no strong affinities to other genera, indicating that they represent phylogenetically isolated lineages or represent remnants of previously larger and earlier diversified groups. The present study forms the foundation for targeted molecular, morphological and phytogeographic studies of the polyphyletic and monotypic genera and particular of clades of genera with interrelations uncovered here for the first time. 相似文献
3.
Yuzo Yamada Hiroko Kawasaki Takashi Nakase Isao Banno 《Bioscience, biotechnology, and biochemistry》2013,77(11):2993-3001
The eleven strains of conidium-forming yeasts classified in the basidiomycetous anamorphic genera Sterigmatomyces, Kurtzmanomyces, Tsuchiyaea and Fellomyces, and the teleomorphic genus Sterigmatosporidium were examined as to the partial sequences of 18S rRNA and 26S rRNA. The positions determined (in Saccharomyces cerevisiae) were 1451 through 1618 of 18S rRNA, and 1618 through 1835 and 470 through 626 of 26S rRNA. The partial sequence determination of positions 470 through 626 of 26S rRNA indicated that T. wingfieldii should be included in the group comprised of Fellomyces and Sterigmatosporidium species. However, the genera Sterigmatomyces, Kurtzmanomyces, Tsuchiyaea and Fellomyces constituted their own separate clusters as to the partial sequences of positions 1451 through 1618 of 18S rRNA and 1618 through 1835 of 26S rRNA. The classification of the conidium-forming yeasts in the four basidiomycetous anamorphic genera mentioned above was proved to be reasonable from the phylogenetic point of view. 相似文献
4.
基于rDNAITS序列分析Alternaria与相似属的关系及A.leucanthemi的分类地位 总被引:6,自引:0,他引:6
对Alternaria,Stemphylium,Ulocladium,Nimbya,Embellisia等五个形态相似属的代表性种进行了5.8S/ITS区段序列测定,连同从GenBank下载的相关有性型Pleospora,Lewia的同项资料,由Neighbor-joining方法构建系统发育树。序列对比结果显示,所有参试的菌株/种的5.8SrDNA序列保守程度较高,ITS序列变化较大,ITS1比ITS2变化更大。由5.8S/ITS构建的系统发育树可以足够将供试菌区分到属;系统发育树显示Alternaria,Ulocladium,Nimbya和Embellisia系统学关系较近,又存在一定的分化;Stemphylium聚在相对较远的分支上,与上述四属关系较远,相对独立进化;有性型的Pleospora与无性型的Stemphylium被聚到一个分支,这种不同阶段菌株在遗传基础上的一致,充分证明所用方法及所选测序的rDNA区段对本类群真菌的分类很有价值。Alternarialeucanthemi位于系统发育树的外侧,较为独立进化,与Alternaria的其它种亲缘关系较远,却与Stemphyliumspp.和Bipolarisspp.关系较近,对其目前的分类地位提出质疑。 相似文献
5.
A novel acidophilic fungus was isolated by an acidic enrichment culture of microbial mats and biofilms collected at an extremely
acidic and high temperature hot spring. In culture studies, this fungus was revealed to produce ascomycetous teleomorph structures.
Molecular phylogenetic study and morphological observation showed this fungus is a new species of the genus Teratosphaeria (Capnodiales, Dothideomycetes) and is phylogenetically close to Acidomyces acidophilus and Bispora sp., which were previously reported as acidophilic anamorphic fungi. This new fungus is described here as a new species of
Teratosphaeria, and its physiological properties adapting to its habitat are demonstrated. This is the first report of a teleomorphic fungus
having highly acidophilic and thermophilic properties. 相似文献
6.
The phylogenetic position of the anamorphic genus Calcarisporiella was investigated. Three isolates of Calcarisporiella, including an authentic strain and a newly obtained isolate, were analyzed phylogenetically using rDNA sequences. The result indicated that Calcarisporiella, which was classified as an ascomycetous anamorph, is a member of Mucoromycotina. It formed an independent clade separated from the other known orders of this subphylum. 相似文献
7.
Chang Sun Kim Takashi Shirouzu Akira Nakagiri Kozue Sotome Nitaro Maekawa 《Mycological Progress》2013,12(4):739-753
Trichoderma eijii and T. pseudolacteum, are described here as new species from Japan. These species were isolated from decaying wood in the Tomakomai Experimental Forest in Hokkaido Prefecture and from bedlogs on shiitake mushroom (Lentinula edodes) farms, respectively. The species were characterized using a combined approach that included cultural studies, holomorph morphology, and phylogenetic analyses of internal transcribed spacer and protein coding gene sequences (RNA polymerase subunit II, translation elongation factor 1-α, endochitinase, and actin). The results of phylogenetic analyses of these gene sequences indicate that T. eijii belongs to the Hamatum clade and is closely related to Hypocrea pezizoides, H. flaviconidia, and H. atroviridis/T. atroviride, from which it differs mainly in part-ascospore size and anamorphic characteristics. Trichoderma pseudolacteum, which was previously recognized as H. lactea sensu Doi, is morphologically distinct from H. lactea (= Hypocrea citrina) and is strongly supported as a separate lineage based on our phylogenetic analyses. 相似文献
8.
Marc Stadler Jacques Fournier Thomas Læssøe Cony Decock Derek Peršoh Gerhard Rambold 《Mycological Progress》2010,9(2):169-179
During a foray to the mountain rainforests of the Democratic Republic of the Congo, a peculiar species of Xylariaceae was
found, which could not be accommodated in any of the existing genera. It is recognised as representative of a new genus, named
Ruwenzoria, owing to the presence of a new combination of teleomorphic and anamorphic characters that are regarded as significant for
generic segregation within the Xylariaceae. Studies on its secondary metabolites in stromata and cultures by high performance
liquid chromatography, coupled with diode array and mass spectrometric detection (HPLC-DAD/MS), and on its phylogenetic affinities
based on 5.8S/ITS rDNA sequence data, respectively, revealed a close relationship of the new taxon to the genera Daldinia and Entonaema, from which it differs by having neither a hollow, gelatinous nor a conspicuously zonate stromatal interior, and an anamorph
featuring enteroblastic rather than holoblastic conidiogenesis. A specimen from the same geographic region, previously identified
as Daldinia bakeri by R.W.G. Dennis was found to constitute a mixture of stromata of Ruwenzoria and an additional, undescribed Daldinia species. The latter fungus is not formally described due to the scantiness of the material, but its morphological characteristics
are illustrated. 相似文献
9.
10.
Lawrey JD Diederich P Nelsen MP Sikaroodi M Gillevet PM Brand AM van den Boom P 《Fungal biology》2011,115(2):176-187
Lichenicolous fungi are obligately lichen-associated organisms that have evolved many times throughout the Ascomycota and Basidiomycota. Approximately 20% of lichenicolous ascomycetes are recognized only from asexual (anamorphic) characteristics, so the phylogenetic position of many groups has never been resolved. Here we present the first molecular phylogeny of Lichenoconium, a genus of strictly asexual, obligately lichenicolous species with broad geographic distributions and diverse host ecologies. We obtained nuclear and mitochondrial rDNA sequences from fungal cultures isolated from four species in the genus, including a new species, Lichenoconium aeruginosum sp. nov., collected in France, Luxembourg and Netherlands. Our multilocus phylogeny supports the monophyly of fungi in the genus Lichenoconium, and places the genus in the Dothideomycetes, an ascomycete class made up mainly of saprobes and plant-associated endophytes and pathogens. There are only a few recognized groups of lichen-formers in the Dothideomycetes, but Lichenoconium is not supported as being closely related to any of these, nor to any other recognized order within the Dothideomycetes. Given that Lichenoconium is but one of over 100 genera of anamorphic lichenicolous fungi, most of which have never been studied phylogenetically, we suggest that asexual lichenicolous fungi may represent novel and evolutionarily significant phylogenetic groups in the Kingdom Fungi. 相似文献
11.
To estimate the phylogenetic position of the strictly anamorphic genus Thysanophora among the class Ascomycetes sensu Kirk et al. and to examine the phylogenetic relationships among T. penicillioides and other Thysanophora species, 18S and 28S rDNA (D1 and D2 regions) sequences of 22 strains of four known and two unidentified Thysanophora species were determined and phylogenetically analyzed. The 18S rDNA analysis suggested that all Thysanophora species examined were members of Eurotiomycetidae, Eurotiales, Trichocomaceae. The 28S rDNA analysis indicated that these
species were clustered together with Chromocleista, Eupenicillium, Geosmithia, and Penicillium assignable to three subgenera – Aspergilloides, Furcatum, and Penicillium. In the Eupenicillium lineage, a monophyly of T. penicillioides, T. longispora, T. taxi, T. canadensis, and T. cf. canadensis was supported by comparatively high bootstrap values. However, the ex-type strain and two strains of T. longispora isolated in Japan were of different phylogenetic positions. Thysanophora sp. was positioned at the base of the Thysanophora clade, although it was not supported by significant bootstrap values. From the results of this study, we consider that two
anamorphic genera, Penicillium and Thysanophora, are clearly distinct in morphology but that they are not phylogenetically separable.
Received: August 13, 2001 / Accepted: January 11, 2002 相似文献
12.
The anamorphic fungus Synchaetomella lunatospora gen. et sp. nov. is described from a culture isolated from leaf litter in Singapore. The genus is characterized by the production
of synnematous conidiomata with brown stipes, phialidic conidiogenesis with terminal and acropleurogenous conidiogenous cells,
and falcate, 1-septate conidia, aggregating in white, mucilaginous heads. Its phylogenetic relationships, inferred from the
partial nuclear small ribosomal subunit (18S) sequence, are with the coelomycetous genera Sphaerographium, Chaetomella, and Hainesia. However, the order and family affinities of this group are unclear. 相似文献
13.
Laura Selbmann Benedetta Turchetti Andrey Yurkov Clarissa Cecchini Laura Zucconi Daniela Isola Pietro Buzzini Silvano Onofri 《Extremophiles : life under extreme conditions》2014,18(4):707-721
In the framework of a large-scale rock sampling in Continental Antarctica, a number of yeasts have been isolated. Two strains that are unable to grow above 20 °C and that have low ITS sequence similarities with available data in the public domain were found. The D1/D2 LSU molecular phylogeny placed them in an isolated position in the genus Taphrina, supporting their affiliation to a not yet described species. Because the new species is able to grow in its anamorphic state only, the species Taphrina antarctica f.a. (forma asexualis) sp. nov. has been proposed to accommodate both strains (type strain DBVPG 5268T, DSM 27485T and CBS 13532T). Lalaria and Taphrina species are dimorphic ascomycetes, where the anamorphic yeast represents the saprotrophic state and the teleomorph is the parasitic counterpart on plants. This is the first record for this genus in Antarctica; since plants are absent on the continent, we hypothesize that the fungus may have focused on the saprotrophic part of its life cycle to overcome the absence of its natural host and adapt environmental constrains. Following the new International Code of Nomenclature for Algae, Fungi and Plants (Melbourne Code 2011) the reorganization of Taphrina–Lalaria species in the teleomorphic genus Taphrina is proposed. We emend the diagnosis of the genus Taphrina to accommodate asexual saprobic states of these fungi. Taphrina antarctica was registered in MycoBank under MB 808028. 相似文献
14.
Rungtiwa Phookamsak Rajesh Jeewon Junfu Li Saranyaphat Boonmee Itthayakorn Promputtha 《Mycological Progress》2018,17(5):647-660
Two novel species of bambusicolous fungi in the genus Kamalomyces, collected from northern Thailand, are described and illustrated herein. Kamalomyces bambusicola and K. thailandicus spp. nov. are typical of the genus Kamalomyces (Tubeufiaceae, Tubeufiales) and are morphologically distinct from known species with respect to their size of ascomata, asci and ascospores, ascospore septation and peridium structure, including the subiculum comprising hyphae on the host surface. Morphological examination reveals that the asexual morph of K. bambusicola is associated with its sexual morph in a subiculum forming dictyochlamydosporous conidia, which are similar to the asexual morph of Chlamydotubeufia. Phylogenetic analyses of combined LSU, ITS and TEF1-α sequence data also support these two species as distinct and confirm their phylogenetic affinities within the Tubeufiaceae. In particular, Kamalomyces shares a close phylogenetic relationship to Helicoma. 相似文献
15.
Three genera of asexual, helical-spored fungi, Helicoma, Helicomyces and Helicosporium traditionally have been differentiated by the morphology of their conidia and conidiophores. In this paper we assessed their phylogenetic relationships from ribosomal sequences from ITS, 5.8S and partial LSU regions using maximum parsimony, maximum likelihood and Bayesian analysis. Forty-five isolates from the three genera were closely related and were within the teleomorphic genus Tubeufia sensu Barr (Tubeufiaceae, Ascomycota). Most of the species could be placed in one of the seven clades that each received 78% or greater bootstrap support. However none of the anamorphic genera were monophyletic and all but one of the clades contained species from more than one genus. The 15 isolates of Helicoma were scattered through the phylogeny and appeared in five of the clades. None of the four sections within the genus were monophyletic, although species from Helicoma sect. helicoma were concentrated in Clade A. The Helicosporium species also appeared in five clades. The four Helicomyces species were distributed among three clades. Most of the clades supported by sequence data lacked unifying morphological characters. Traditional characters such as the thickness of the conidial filament and whether conidiophores were conspicuous or reduced proved to be poor predictors of phylogenetic relationships. However some combinations of characters including conidium colour and the presence of lateral, tooth-like conidiogenous cells did appear to be predictive of genetic relationships. 相似文献
16.
Kuraishi H Itoh M Katayama Y Ito T Hasegawa A Sugiyama J 《Antonie van Leeuwenhoek》2000,77(2):179-186
The ubiquinone (coenzyme Q) systems were determined for 176 teleomorphic isolates, 14 anamorphic isolates, and three samples of fruit-bodies of Dendrosphaera eberhardtii, which belonged to Eurotiales, Onygenales, and related taxa. In Eurotiales, Ascosphaera had Q-9, whereas Bettsia had Q-10. All isolates of Monascaceae had the Q-10 system, whereas those of four genera of Pseudeurotiaceae had the Q-10(H2) system. The Q-10(H2) system was found in genera of Trichocomaceae, except for Aspergillus, Penicillium, Paecilomyces, and their related taxa. However, Thermoascus had the Q-9 system. In Onygenales, members of Arthrodermataceae had Q-9, and those of Gymnoascaceae had Q-10(H2). Isolates of Myxotrichaceae were characterized by Q-10(H2) with few exceptions, which had Q-10. The quinones of Onygenaceae belonged to complex systems, i.e., Q-9, 0-10 and 0-10(H2), and a combination of two systems. Families Onygenaceae and Trichocomaceae are likely a phylogenetic heterogeneity. Ubiquinone analysis provides a very useful criterion of great promise for classifying eurotialean taxa and also for identifying their isolates. 相似文献
17.
Although dark septate fungal endophytes (DSE) occur widely in association with plant roots in cold-stressed habitats, little is known of the taxonomic status of DSE in Antarctica. Here we investigate the phylogenetic affinities of DSE colonising the roots of Colobanthus quitensis and Deschampsia antarctica, the two maritime Antarctic vascular plant species. Two hundred and forty-three DSE were isolated from roots collected from 17 sites across a 1 470 km transect through maritime and sub-Antarctica. The ITS1-5.8S-ITS2 nuclear ribosomal gene cluster of representative isolates was sequenced, and the sequences were recovered in 10 sequence groups and sub-groups. Nine of the sequence groupings could be placed in the Helotiales and the remaining one showed high homology to a large number of currently unassigned anamorphic ascomycete sequences. Of the Helotiales, Leptodontidium orchidicola, Rhizoscyphus ericae and species of Tapesia and Mollisia could be confidently identified. This study demonstrates that members of the Helotiales, including several widely-recognised DSE genera, commonly colonise the roots of C. quitensis and D. antarctica in the Antarctic. 相似文献
18.
Salman V Amann R Girnth AC Polerecky L Bailey JV Høgslund S Jessen G Pantoja S Schulz-Vogt HN 《Systematic and applied microbiology》2011,34(4):243-259
The colorless, large sulfur bacteria are well known because of their intriguing appearance, size and abundance in sulfidic settings. Since their discovery in 1803 these bacteria have been classified according to their conspicuous morphology. However, in microbiology the use of morphological criteria alone to predict phylogenetic relatedness has frequently proven to be misleading. Recent sequencing of a number of 16S rRNA genes of large sulfur bacteria revealed frequent inconsistencies between the morphologically determined taxonomy of genera and the genetically derived classification. Nevertheless, newly described bacteria were classified based on their morphological properties, leading to polyphyletic taxa. We performed sequencing of 16S rRNA genes and internal transcribed spacer (ITS) regions, together with detailed morphological analysis of hand-picked individuals of novel non-filamentous as well as known filamentous large sulfur bacteria, including the hitherto only partially sequenced species Thiomargarita namibiensis, Thioploca araucae and Thioploca chileae. Based on 128 nearly full-length 16S rRNA-ITS sequences, we propose the retention of the family Beggiatoaceae for the genera closely related to Beggiatoa, as opposed to the recently suggested fusion of all colorless sulfur bacteria into one family, the Thiotrichaceae. Furthermore, we propose the addition of nine Candidatus species along with seven new Candidatus genera to the family Beggiatoaceae. The extended family Beggiatoaceae thus remains monophyletic and is phylogenetically clearly separated from other related families. 相似文献
19.
Desiree Johnson Nigel L. Hywel-Jones Joseph F. Bischoff Joseph W. Spatafora 《Mycological Research》2009,113(3):279-289
Torrubiella is a genus of arthropod-pathogenic fungi that primarily attacks spiders and scale insects. Based on the morphology of the perithecia, asci, and ascospores, it is classified in Clavicipitaceae s. lat. (Hypocreales), and is considered a close relative of Cordyceps s. 1., which was recently reclassified into three families (Clavicipitaceae s. str., Cordycipitaceae, Ophiocordycipitaceae) and four genera (Cordyceps s. str, Elaphocordyceps, Metacordyceps, and Ophiocordyceps). Torrubiella is distinguished morphologically from Cordyceps s. lat. mainly by the production of superficial perithecia and the absence of a well-developed stipitate stroma. To test and refine evolutionary hypotheses regarding the placement of Torrubiella and its relationship to Cordyceps s. lat., a multi-gene phylogeny was constructed by conducting ML and Bayesian analyses. The monophyly of Torrubiella was rejected by these analyses with species of the genus present in Clavicipitaceae, Cordycipitaceae, and Ophiocordycipitaceae, and often intermixed among species of Cordyceps s. lat. The morphological characters traditionally used to define the genus are, therefore, not phylogenetically informative, with the stipitate stromata being gained and/or lost several times among clavicipitaceous fungi. Two new genera (Conoideocrella, Orbiocrella) are proposed to accommodate two separate lineages of torrubielloid fungi in the Clavicipitaceae s. str. In addition, one species is reclassified in Cordyceps s. str. and three are reclassified in Ophiocordyceps. The phylogenetic importance of anamorphic genera, host affiliation, and stipitate stromata is discussed. 相似文献
20.
Arthropod pests in forest and agricultural systems are affected by many pathogenic organisms. Among them, entomopathogenic
fungi are the one most common control agents that regulate their populations. This review compiles the information available
from Argentina and Brazil about the entomopathogenic fungi occurring in agricultural and natural environments. The scientific
names of the fungi are listed according to the latest phylogenetically based classification of fungi. We present an updated
list of arthropod-pathogenic fungi occurring in 15 of the 23 provinces of Argentina and 20 of the 27 states of Brazil based
on published literature and our personal observations. The list includes a total of 114 fungal species from 53 genera: of
Blastocladiomycetes (2 genera), Entomophthorales (8 genera), Harpellales (13 genera), and a diverse assortment of ascomycetes
(primarily from Hypocreales) in 22 anamorphic and 5 teleomorphic genera. In the both countries, molecular studies on arthropod-pathogenic
fungi are still in their early stages and have focused primarily on intraspecific variability and adequate generic assignment.
This listing seeks to encourage more active collection and characterization of these fungi by both traditional and molecular
approaches from the obviously rich but underexplored flora of fungi affecting arthropods throughout this large region of South
America. 相似文献