共查询到20条相似文献,搜索用时 15 毫秒
1.
Background
The subcloning of a DNA fragment from an entry vector into a destination vector is a routinely performed task in molecular biology labs.Results
We here present a novel benchtop procedure to achieve rapid recombination into any destination vector of choice with the sole requirement of an endonuclease recognition site. The method relies on a specifically designed entry vector and the combined action of type II and type IIs endonucleases with ligase. The formulation leads to accumulation of a single stable cloning product representing the desired insert carrying destination vector.Conclusion
The described method provides a fast single step procedure for routine subcloning from an entry vector into a series of destination vectors with the same restriction enzyme recognition site. 相似文献2.
Philippos A Papathanos Nikolai Windbichler Miriam Menichelli Austin Burt Andrea Crisanti 《BMC molecular biology》2009,10(1):65-13
Background
Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae. 相似文献3.
Background
A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules. 相似文献4.
David M Markusic Niek P van Til Johan K Hiralall PJ Oude Ronald Elferink Jurgen Seppen 《BMC biotechnology》2009,9(1):85
Background
Lentiviral vectors are well suited for gene therapy because they can mediate long-term expression in both dividing and nondividing cells. However, lentiviral vectors seem less suitable for liver gene therapy because systemically administered lentiviral vectors are preferentially sequestered by liver macrophages. This results in a reduction of available virus and might also increase the immune response to the vector and vector products. 相似文献5.
6.
Bart GJ Knols Basilio N Njiru Evan M Mathenge Wolfgang R Mukabana John C Beier Gerry F Killeen 《Malaria journal》2002,1(1):19-13
Background
The development and implementation of innovative vector control strategies for malaria control in Africa requires in-depth ecological studies in contained semi-field environments. This particularly applies to the development and release of genetically-engineered vectors that are refractory to Plasmodium infection. Here we describe a modified greenhouse, designed to simulate a natural Anopheles gambiae Giles ecosystem, and the first successful trials to complete the life-cycle of this mosquito vector therein. 相似文献7.
Janneth Rodrigues Neema Agrawal Anil Sharma Pawan Malhotra Tridibes Adak Virander S Chauhan Raj K Bhatnagar 《BMC molecular biology》2007,8(1):33
Background
The main vector for transmission of malaria in India is the Anopheles culicifacies mosquito species, a naturally selected subgroup of which is completely refractory (R) to transmission of the malaria parasite, Plasmodium vivax; 相似文献8.
Martine Geraerts Sofie Willems Veerle Baekelandt Zeger Debyser Rik Gijsbers 《BMC biotechnology》2006,6(1):34
Background
Lentiviral vectors are efficient vehicles for stable gene transfer in dividing and non-dividing cells. Several improvements in vector design to increase biosafety and transgene expression, have led to the approval of these vectors for use in clinical studies. Methods are required to analyze the quality of lentiviral vector production, the efficiency of gene transfer and the extent of therapeutic gene expression. 相似文献9.
Background
The challenge of remote homology detection is that many evolutionarily related sequences have very little similarity at the amino acid level. Kernel-based discriminative methods, such as support vector machines (SVMs), that use vector representations of sequences derived from sequence properties have been shown to have superior accuracy when compared to traditional approaches for the task of remote homology detection. 相似文献10.
Potential biological control of Erwinia tracheiphila by internal alimentary canal interactions in Acalymma vittatum with Pseudomonas fluorescens
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D.C. Roberts S.J. Fleischer J.M. Sakamoto J.L. Rasgon 《Journal of applied microbiology》2018,125(4):1137-1146
Aims
We aim to determine if Pseudomonas fluorescens is a viable biological control for Erwinia tracheiphila within the insect vector, Acalymma vittatum.Methods and Results
Pseudomonas fluorescens secreted fluorescein and inhibited growth of E. tracheiphila in disc diffusion assays. To determine if this antagonism was conserved within the insect vector, we performed in vivo assays by orally injecting beetles with bacterial treatments and fluorescent in situ hybridization to determine bacterial presence within the alimentary canal.Conclusions
Pseudomonas fluorescens inhibited the growth of E. tracheiphila on a nutrient‐limiting medium. In situ experiments demonstrated that P. fluorescens is maintained within the alimentary canal of the beetle for at least 4 days, and co‐occurred with E. tracheiphila. When beetles were first presented with Pseudomonas and then challenged with E. tracheiphila, E. tracheiphila was not recovered via FISH after 4 days. These data suggest that P. fluorescens has potential as a biological control agent to limit E. tracheiphila within the insect vector.Significance and Impact of the Study
This is a novel approach for controlling E. tracheiphila that has the potential to decrease reliance on insecticides, providing a safer environment for pollinators and growers. 相似文献11.
Matthias Paar Dieter Klein Brian Salmons Walter H Günzburg Matthias Renner Daniel Portsmouth 《BMC molecular biology》2009,10(1):8-14
Background
The recent advent of murine leukaemia virus (MLV)-based replication-competent retroviral (RCR) vector technology has provided exciting new tools for gene delivery, albeit the advances in vector efficiency which have been realized are also accompanied by a set of fresh challenges. The expression of additional transgene sequences, for example, increases the length of the viral genome, which can lead to reductions in replication efficiency and in turn to vector genome instability. This necessitates efforts to analyse the rate and mechanism of recombinant emergence during the replication of such vectors to provide data which should contribute to improvements in RCR vector design. 相似文献12.
Background
Pseudomonas aeruginosa encodes a putative topoisomerase with sequence similarity to the eukaryotic type IB topoisomerase from Vaccinia virus. Residues in the active site are conserved, notably Tyr292 which would be predicted to form the transient covalent bond to DNA. 相似文献13.
Shan Goh Andrea Camattari Daniel Ng Ruth Song Kevin Madden Janet Westpheling Victor VT Wong 《BMC biotechnology》2007,7(1):72
Background
The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One way to increase AP-3 production would be to constitutively express selected genes so as to relieve bottlenecks in the biosynthetic pathway; however, an integrative expression vector for A. pretiosum is lacking. The aim of this study was to construct a vector for heterologous gene expression in A. pretiosum. 相似文献14.
Luísa DP Rona Carlos J Carvalho-Pinto Camila J Mazzoni Alexandre A Peixoto 《BMC evolutionary biology》2010,10(1):91
Background
Anopheles cruzii is the primary human Plasmodium vector in southern and southeastern Brazil. The distribution of this mosquito follows the coast of the Brazilian Atlantic Forest. Previous studies indicated that An. cruzii is a complex of cryptic species. 相似文献15.
Background
Over the last decade several prediction methods have been developed for determining the structural and functional properties of individual protein residues using sequence and sequence-derived information. Most of these methods are based on support vector machines as they provide accurate and generalizable prediction models. 相似文献16.
Cynthia M Balion Carolyn Benson Parminder S Raina Alexandra Papaioannou Christopher Patterson Afisi S Ismaila 《BMC neurology》2007,7(1):38
Background
The pathological processes underlying dementia are poorly understood and so are the markers which identify them. Carnosinase is a dipeptidase found almost exclusively in brain and serum. Carnosinase and its substrate carnosine have been linked to neuropathophysiological processes. 相似文献17.
Zhijun Wang Li Xiang Junjie Shao Alicja Węgrzyn Grzegorz Węgrzyn 《Microbial cell factories》2006,5(1):34-18
Background
Although understanding of physiological interactions between plasmid DNA and its host is important for vector design and host optimization in many biotechnological applications, to our knowledge, global studies on plasmid-host interactions have not been performed to date even for well-characterized plasmids. 相似文献18.
Background
Protein remote homology detection and fold recognition are central problems in bioinformatics. Currently, discriminative methods based on support vector machine (SVM) are the most effective and accurate methods for solving these problems. A key step to improve the performance of the SVM-based methods is to find a suitable representation of protein sequences. 相似文献19.
Kaya T Ishizawa Hiroaki Kumano Atsushi Sato Hiroshi Sakura Yasuhiko Iwamoto 《BioPsychoSocial medicine》2010,4(1):1
Background
This study was performed to examine whether patients with type 2 diabetes have cognitive deficits associated with the prefrontal cortex (PFC). 相似文献20.
Camila J Mazzoni Nataly A Souza Claudia Andrade-Coelho Charalambos P Kyriacou Alexandre A Peixoto 《BMC evolutionary biology》2006,6(1):85-11