玉竹的组织培养与快速繁殖

以玉竹[Polygonatum odoratum (Mill.) Druce]根状茎、叶片和茎段为外植体,于附加不同激素配比的MS培养基中诱导愈伤组织、不定芽和不定根,探讨增殖培养和植株再生的条件.结果表明,叶片和茎段外植体诱导愈伤组织和芽的分化率很低;而根状茎外植体易于培养,有较高的诱导率和增殖倍数,其愈伤组织、不定芽和不定根的诱导率分别可达87%、90%和99%以上.适宜根状茎外植体愈伤组织诱导的培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA,有利于增殖和丛生芽分化的培养基为MS+2.0 mg/L 6-BA+0.5 mg/L IBA和MS+3.0 mg/L 6-BA+0.1 mg/L NAA,而1/2MS+3.0～5.0 mg/L NAA适宜诱导试管苗生根培养.试管苗的移栽成活率可达85%以上.     

商陆离体再生体系的构建

目的:建立商陆离体再生体系。方法:选取商陆的幼茎、茎节、叶片、叶柄和顶芽为外植体,以MS作为基本培养基,通过添加不同浓度配比的植物生长调节剂分别进行愈伤组织、丛生芽和生根诱导,筛选商陆离体再生体系方案。结果:顶芽和幼茎为外植体诱导的愈伤组织出愈时间早,愈伤组织质量高,以培养基MS+6-BA0.5 mg/L+2.4-D 0.5 mg/L的诱导率最高,达到100%;其中,只有以顶芽产生的愈伤组织才能分化出丛生芽,芽分化培养基为MS+6-BA 2.0 mg/L+NAA 0.25 mg/L,诱导率为98%;诱导生根的适宜培养基为1/2 MS+NAA 0.3 mg/L,诱导率达100%。结论:建立和完善了商陆离体再生体系方案,为商陆遗传转化体系的构建奠定了基础。     

蚊净香草快速繁殖研究

以蚊净香草嫩叶和叶柄为外植体进行培养,筛选合适的外植体与诱导、增殖和生根的最佳培养基。结果表明,叶片外植体在MS+6-BA 2.0mg/L+NAA 0.5mg/L培养基最适于诱导愈伤组织和不定芽;MS+6-BA 1.0mg/L+NAA 0.3mg/L培养基有利于不定芽增殖;无根苗在含有低浓度无机盐和生长素水平的生根培养基上易生根,生根率高达100%。     

TDZ和CPPU对红掌快速繁殖的影响

以红掌带主脉叶切片、叶柄切段为外植体,研究TDZ和CPPU对其快速繁殖的影响。结果表明,在愈伤组织诱导上,与6-BA相比,TDZ和CPPU具有更强的诱导外植体脱分化活性;TDZ 1.0mg/L和6-BA0.5mg/L配比是诱导红掌外植体脱分化的最佳配方,与其它培养基比较,愈伤组织诱导率提高50.0%~166.0%。在附加TDZ 0.3mg/L+NAA 0.1~0.4mg/L或CPPU 0.3mg/L+NAA 0.1~0.4mg/L的MS培养基上,愈伤组织均能很好分化,并能长成完整植株。     

康乃馨茎段愈伤组织诱导及植株再生(简报)

以康乃馨无菌苗茎段为外植体,在MS 6-BA 0.5mg/L NAA0.2mg/L培养基上诱导产生愈伤组织,愈伤组织在MS 6-BA 0.5mg/L NAA 0.2mg/L培养基上诱导芽效果较好,芽苗在1/2MS NAA 0.1mg/L培养基上可诱导生根。     

紫色大花矮牵牛组织培养与植株再生

矮牵牛叶片外植体在MS+6-BA 1.0mg/L+NAA 0.1mg/L培养基上培养3周后产生致密的浅绿色愈伤组织;转入芽分化培养基MS+6-BA 0.5mg/L+4-PU 0.5mg/L+NAA 0.1mg/L 1周后,从愈伤组织表面不断分化产生幼芽;待幼芽长至3cm时转接至生根培养基1/2MS+NAA 1.0 mg/L+GA₃0.5mg/L中生根,长成完整植株。     

如意皇后粗肋草愈伤组织及丛生芽诱导培养基优化

本研究以粗肋草‘Red Valentine’带侧芽的根茎为外植体,研究不同培养基类型、不同外源激素及浓度、不同转接周期对其愈伤组织及丛生芽诱导的影响,进而优化如意愈伤组织和丛生芽诱导培养基。结果表明:最佳愈伤组织诱导培养基为MS+0.5 mg/L TDZ+2.0 mg/L 2,4-D和1/2MS+0.5 mg/L TDZ+2.5 mg/L 2,4-D;最佳愈伤组织分化培养基为1/2 MS+0.4 mg/L TDZ,最佳转接周期为30 d;最佳丛生芽诱导培养基为5.0 mg/L6-BA+0.2 mg/L NAA和1/2 MS+0.5 mg/L TDZ,最佳转接周期为30 d。优化培养基后愈伤组织及丛生芽诱导效率更高。本研究通过设计优化试验,筛选出了愈伤组织诱导及丛生芽诱导的最佳培养基,为粗肋草属植物的快速繁殖及规模化生产提供了一定的理论指导。     

金线莲外植体筛选及愈伤组织诱导研究

以金线莲茎段、叶片、茎片、不定芽为试材,分别在添加6-BA、ZT、NAA、KT 5个不同处理的MS及1/2MS培养基上培养,诱导愈伤组织。结果表明,以茎段、茎片、不定芽为外植体,在MS + 6-BA 2.0 mg/L + NAA0.5 mg/L、MS + NAA 2.0 mg/L + KT 0.1 mg/L和MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + ZT 0.2 mg/L培养基中培养,均能成功诱导愈伤组织。不定芽为诱导愈伤组织最佳外植体,最佳培养基为MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + KT 0.2 mg/L。     

川芎高频再生体系的建立

为建立川芎(Ligusticum chuanxiong Hort.)高频再生体系,优化了诱导和分化培养基及培养条件。以叶柄为外植体,以MS为基本培养基,KT 2.0 mg/L+IAA 0.5 mg/L的激素组合对不定芽分化最有利。在此基础上,针对外植体来源、培养条件和愈伤组织继代时间3个因素进行优化。结果表明:采用川芎无菌苗叶柄作为外植体,黑暗条件下诱导出愈伤组织,再在光照下继代培养15 d后转入分化培养基中对不定芽诱导最为有利,分化率为44.4%。分化后得到的不定芽在含NAA 0.5 mg/L和IBA 0.5 mg/L的 1/2MS培养基上生根率达90%,移栽存活率为95%。     

川芎高频再生体系的建立

为建立川芎(Ligusticum chuanxiong Hort.)高频再生体系,优化了诱导和分化培养基及培养条件.以叶柄为外植体,以MS为基本培养基,KT2.0 mg/L+ IAA0.5mg/L的激素组合对不定芽分化最有利.在此基础上,针对外植体来源、培养条件和愈伤组织继代时间3个因素进行优化.结果表明:采用川芎无菌苗叶柄作为外植体,黑暗条件下诱导出愈伤组织,再在光照下继代培养15d后转入分化培养基中对不定芽诱导最为有利,分化率为44.4％.分化后得到的不定芽在含NAA0.5 mg/L和IBA 0.5 mg/L的1/2MS培养基上生根率达90％,移栽存活率为95％.     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.     

杆状病毒出芽病毒粒子膜融合蛋白的研究进展

杆状病毒是一类感染节肢动物的病原微生物,其基因组为双链环状DNA,大小为80～180kb.     

Inception of the Modern Public Health System in China and Perspectives for Effective Control of Emerging Infectious Diseases: In Commemoration of the 140th Anniversary of the Birth of the Plague Fighter Dr. Wu Lien-Teh

In this article, we systematically review Dr. Wu Lien-Teh's academic achievements and outstanding contributions in the prevention and control of the plague epidemic in northeast China and introduce the development of the earliest public health epidemic prevention system in China in order to commemorate the 140th anniversary of Dr. Wu Lien-Teh's birth. We hope that this article will provide insights into the effective prevention and control of emerging infectious diseases as well as the current worldwide pandemic of COVID-19, facilitating the improvement and development of public health systems in China and around the globe.