克拉豆的组织培养与快速繁殖

1植物名称克拉豆(Cratylia argentea Benth.)。2材料类别幼嫩种子。3培养条件(1)种子萌发培养基:MS基本培养基;(2)分化培养基:MS 6-BA3mg·L-1(单位下同) NAA0.3;(3)生根培养基:1/2MS NAA1.0     

垂花火鸟蕉的组织培养

1植物名称垂花火鸟蕉(HeliconiarostrataRuiz&Pavon)。2材料类别种子长成的无菌苗茎段。3培养条件种子萌发培养基:1/2MS培养基。芽启动培养基:(1)1/2MS+6-BA3mg·L-1(单位下同)+NAA0.5+1%活性炭;(2)1/2MS+6-BA5+NAA0.5+1%活性炭;(3)1/2MS+6-BA10+NAA0.5+1%活性炭。芽增殖培养基:(4)MS+6-BA3+NAA0.3。生根培养基:(5)MS+NAA0.5+0.5%活性炭。以上培养基均添加7g·L-1琼脂粉和30g·L-1蔗糖,pH5.8,在121℃下高压灭菌20min。培养温度为(25±2)℃,光照时间12h·d-1,光照强度50μmol·m-2·s-1。4生长与分化情况4.1无菌材料的获得…     

单色蝴蝶草的组织培养和快速繁殖

1植物名称单色蝴蝶草(Torenia concolor Lindl.)。2材料类别叶片。3培养条件丛生芽诱导培养基:(1)MS 6-BA 2.0 mg·L~(-1)(单位下同);(2)MS 6-BA 2.0 NAA 0.2;(3)MS 6-BA 2.0 NAA 0.5。生根培养基:(4) 1/2MS;(5)1/2MS NAA 0.2;(6)1/2MS NAA     

古代稀的组织培养和快速繁殖

1植物名称古代稀(GodetiagrandifloraLindl.)(王意成2005)。2材料类别无菌萌发的种子苗。3培养条件种子萌发培养基:(1)MS;芽诱导和分化培养基:(2)MS+6-BA2mg·L-1(单位下同),(3)MS+6-BA2+NAA0.1,(4)MS+6-BA2+NAA0.2;生根培养基:(5)1/2MS+IBA1,(6)1/2MS+IBA2。上述培养基添加3%蔗糖、0.8%琼脂粉,pH5.8￣6.2。培养温度为25℃,光照时间12h·d-1,光照强度27μmol·m-2·s-1。4生长与分化情况4.1无菌种子苗的获得将种子用70%的酒精消毒50s,经0.1%升汞消毒4min后,用无菌水冲洗3￣6次,播种于培养基(1)上。待种子萌发的幼苗高5￣6cm以…     

皱叶山苏的组织培养和植株再生

1植物名称皱叶山苏(Asplenium antiquum Makinocv‘.Victoria’)。2材料类别孢子。3培养条件孢子萌发及原叶体形成的培养基:(1)1/2MS。丛芽分化及增殖培养基:(2)1/2MS;(3)1/2MS 6-BA0.5mg·L-1(单位下同) NAA0.1;(4)1/2MS 6-BA1.0 NAA0.1     

紫斑蝴蝶草的组织培养和快速繁殖

1 植物名称 紫斑蝴蝶草（Torenia fordii Hook. f.）。 2 材料类别 种子萌发形成的叶片。 3 培养条件 不定芽诱导培养基：（1）MS＋CPPU（氯吡苯脲,forchlorfenuron）0.01mg·L^-1（单位下同）;（2）MS＋CPPU0.05;（3）MS＋CPPU0.10;（4）MS＋TDZ（噻苯隆,thidiazuron）0.01;（5）MS＋TDZ0.05;（6）MS＋TDZ0.10。     

黑果枸杞的组织培养

1植物名称黑果枸杞(Lycium ruthenicum Murr.). 2材料类别带腋芽的嫩茎段. 3培养条件诱导分化培养基:(1)MS 6-BA 1.0mg·L-1(单位下同) NAA 0.2,(2)MS 6-BA 0.5 NAA0.2;芽增殖培养基:(3)MS 6-BA 0.5 NAA 0.1(4)MS 6-BA 0.5 NA 0.2;生根培养基为:(5)1/2MS NAA 0.1.以上诱导分化及增殖培养基均加2.5%蔗糖和0.8%琼脂,生根培养基的蔗糖和琼脂量减半,pH 5.8～6.0.培养温度为23～25℃,光照时间10～12 h·d-1,光照度为2000 1x.     

球花石斛的组织培养与快速繁殖

1植物名称球花石斛(Dendrobium thyrsiflorum Rchb.f.)。2材料类别种子。3培养条件种子萌发培养基:(1)1/2VW 100 mL·L~(-1)香蕉汁:(2)VW 100 mL·L~(-1)香蕉汁。原球茎继代增殖培养基:(3)VW KT 0.1 IBA 0.02 mg·L~(-1)(单位下同) 100 mL·L~(-1)香蕉汁:(4)VW KT 1.0 IBA 0.02 100 mL·L~(-1)香蕉汁。壮苗及生根培养基:(5)3 g·L~(-1)花宝1号(台和园艺企业股份有限公     

凤蝶兰的组织培养与快速繁殖

1植物名称凤蝶兰[Papilionanthe teres(Roxb.) Schltr.]。2材料类别种子。3培养条件种子萌发培养基:(1)MS:(2)MS 0.1%活性炭:(3)MS 椰子水100mL·L~(-1)(单位下同);(4)1/2MS:(5)1/2MS 0.1%活性炭;(6)1/2 MS 椰子水100:(7)1/2MS 0.2mg·L~(-1)NAA。以上培养基均添加7g·L~(-1)琼脂粉和30g·L~(-1)蔗糖,pH 5.8,在121℃下高压灭菌20min。培养温度为(25 2)℃,光照12h·d~(-1),光照度50μmol·m~(-2)·s~(-1)。     

Foliar blight in Centrosema pubescens (Benth.) in southwestern Nigeria

The etiology and epidemiology of leaf blight of centro (Centrosema pubescens Benth.) was investigated at Ibadan, southwestern Nigeria, in the lowland humid tropics. Rhizoctonia solani was associated with the leaf blight of centro. The pathogen was harboured by Rottboellia cochinchiensis, a weed growing within and outside the centro plot. A high inoculum population of 4.0 ± 0.3 × 106 colony forming units/g soil was estimated in the soil. The seed of centro were free of R. solani. Field infection of the disease occurs during the peak of the rainy season beginning in patches which later spread, causing extensive defoliation of the sward. This revised version was published online in August 2006 with corrections to the Cover Date.     

毛酸浆的组织培养

1植物名称 毛酸浆（PhysalispubescensL．）。 2材料类别种子长成的无菌苗的子叶。     

Development of the nitrogen fixing apparatus in the legumes,Centrosema pubescens Benth., and Vigna unguiculata L. Walp.

The sequence of events leading up to the establishment of symbiotic nitrogen-fixation were studied in two tropical legumes, Centrosema pubescens Benth, and Vigna unguiculata L. Walp. Parameters measured included fresh and dry weights, chlorophyll and leghaemoglobin contents, as well as the activities of NADH-nitrate reductase (EC 1.6.6.1), and nitrogenase (nitric-oxide reductase-EC 1.7.99.2) in plants that were inoculated with suitable rhizobia or which were watered with potassium nitrate. Dry weight and photosynthetic activity of both species followed the sigmoidal pattern which is characteristic of most plants. Growth was little different in either a qualitative or quantitative sense whether nitrogen was supplied as nitrate or through dinitrogen fixation. Although the biochemical sequence of events was dependent on the limiting sensitivities of the individual assays used, the data suggest that nitrate reductase is the first measurable enzymatic activity in the nodules (and roots), followed by acetylene reduction and leghaemoglobin in that order. It is possible therefore, that low levels of symbiotic nitrogen fixation occur in the nodules in the absence of leghaemoglobin. Nitrate reductase activity in C. pubescens nodules was negatively exponentially correlated with nitrogenase activity of the same nodules, suggesting a changing metabolism in old nodules. These data are discussed in terms of environmental and physical factors known to control nitrogen fixation.     

凉粉草的组织培养及快速繁殖

1植物名称凉粉草(Mesona chinensis Benth.). 2材料类别茎尖及带腋芽的茎段. 3培养条件基本培养基为MS.(1)丛芽分化培养基:MS 6-BA 1.0 mg·L-1(单位下同) NAA 0.1;(2)增殖培养基:MS 6-BA 0.5 NAA 0.1;(3)生根培养基:MS NAA 0.5.以上培养基均附加3%白糖、0.7%琼脂,pH 5.8.培养温度为25℃左右,光照时间14 h·d-1,光强为20～30 μmol·m-2·s-1.     

玉叶金花的组织培养和植株再生

1　植物名称　玉叶金花 (Ｍｕｓｓａｅｎｄａｐｕｂｅｓｃｅｎｓ)。2　材料类别　嫩茎、幼叶。3　培养条件　以ＭＳ为基本培养基。诱导愈伤组织附加 :( 1 )ＮＡＡ 0 .2ｍｇ·Ｌ- 1 (单位下同 ) ＫＴ0 .2 5 6 ＢＡ 0 .1 ;( 2 )ＮＡＡ 0 .5 ＫＴ 0 .2 5 6 ＢＡ0 .1 ;( 3)ＮＡＡ 0 .5 6 ＢＡ 0 .1 ;( 4 ) 2 ,4 Ｄ 0 .2 ＫＴ0 .1。继代培养基附加 :( 5 ) 6 ＢＡ 4 ＫＴ0 .2 5 ＩＡＡ 0 .5。不定芽诱导及增殖培养基附加 :( 6)6 ＢＡ 4 ＫＴ 0 .5 ＩＡＡ 0 .1。生根培养基 :( 7)1 /2ＭＳ ＩＢＡ 0 .5。上述培养基均含 0 .7%琼脂、3%蔗…     

毛冬青的组织培养与快速繁殖

1植物名称 毛冬青（Ilex pubescens Hook．et Am．）。 2材料类别 成熟果实。 3培养条件 基本培养基为1／2MS。（1）种子无菌发芽培养基：1／2MS＋6-BA0．5mg．L-1（单位下同）＋IBA0．2;（2）丛芽增殖培养基：1／2MS＋6-BA1．0＋IBA0．2;（3）生根培养基：1／2MS＋6-BA1．0＋NAA0．5。     

粉葛的离体培养和无糖生根

1植物名称粉葛(Pueraria thomsonii Benth),又名甘葛藤. 2材料类别幼嫩的茎段、茎尖. 3培养条件丛芽诱导培养基:(1)MS 6-BA 0.2mg·L-1(单位下同) NAA 0.2;(2)MS 6-BA 0.5 NAA 0.2;(3)MS 6-BA 1.0 NAA 0.2.继代增殖培养基:(4)MS 6-BA 0.3 NAA 0.3;(5)MS 6-BA0.6 NAA 0.3.无糖生根培养基:(6)MS大量元素 NAA 0.2.粉葛的生根利用箱式无糖培养专利技术[1]进行,基质为蛭石,在培养基中不添加糖分,以C O2作为碳源,培养箱中C O2浓度为0.1%～0.12%,空气流速为2 L·min-1,光照度为5 000lx.在丛芽诱导和继代阶段,培养基中的糖浓度均为3.0%,琼脂的用量为6.0 g·L-1,pH 5.6～5.8.培养温度24～26℃,光照时间10～12 h·d-1,光照度1 500～2000 lx.     

裂叶荆芥的组织培养和快速繁殖

1植物名称裂叶荆芥[Schizonepeta tenuifolia (Benth.)Briq.],又称假苏、四棱杆蒿。2材料类别种子无菌萌发苗的叶和茎段。3培养条件种子萌发培养基:(1)MS;不定芽