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1.
A novel nitrogen-fixing bacterium, BJ-18 T, was isolated from wheat rhizosphere soil. Strain BJ-18 T was observed to be Gram-positive, facultatively anaerobic, motile and rod-shaped (0.4–0.9 μm × 2.0–2.9 μm). Phylogenetic analysis based on a partial nifH gene sequence and an assay for nitrogenase activity showed its nitrogen-fixing capacity. Phylogenetic analysis based on full 16S rRNA gene sequences suggested that strain BJ-18 T is a member of the genus Paenibacillus. High similarity of 16S rRNA gene sequence was found between BJ-18 T and Paenibacillus peoriae DSM 8320 T (99.05 %), Paenibacillus jamilae DSM 13815 T (98.86 %), Paenibacillus brasiliensis DSM 13188 T (98.55 %), Paenibacillus polymyxa DSM 36 T (98.74 %), Paenibacillus terrae DSM 15891 T (97.99 %) and Paenibacillus kribbensis JCM 11465 T (97.92 %), whereas the similarity was below 96.0 % between BJ-18 T and the other Paenibacillus species. DNA–DNA relatedness between strain BJ-18 T and P. peoriae DSM 8320 T, P. jamilae DSM 13815 T, P. brasiliensis DSM 13188 T, P. polymyxa DSM 36 T, P. kribbensis JCM 11465 T and P. terrae DSM 15891 T was determined to be 43.6 ± 2.7, 34.2 ± 5.3, 47.9 ± 6.6, 36.8 ± 3.5, 27.4 ± 4.3 and 23.6 ± 4.1 % respectively. The DNA G+C content of BJ-18 T was determined to be 45.8 mol %. The major fatty acid was identified as anteiso-C 15:0 (67.1 %). The polar lipids present in strain BJ-18 T were identified as diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The phenotypic and genotypic characteristics, and DNA–DNA relatedness data, suggest that BJ-18 T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus beijingensis sp. nov. (Type strain BJ-18 T=DSM25425 T=CGMCC 1.12045 T) is proposed. 相似文献
3.
Two strains HN-1T and 39 were isolated from rhizospheres of different plants grown in different regions of PR China. The two strains exhibited high nitrogenase activities and possessed almost identical 16S rRNA gene sequences. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two strains were 99.9 and 99.8%, respectively, suggesting that they belong to one species. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strains HN-1T and 39 are the members of the genus Paenibacillus and both strains exhibited 99.5% similarity to Paenibacillus stellifer DSM 14472T and the both strains represented a separate lineage from all other Paenibacillus species. However, the ANI of type strain HN-1T with P. stellifer DSM 14472T was 90.69, which was below the recommended threshold value (<?95–96% ANI). The dDDH showed 42.1% relatedness between strain HN-1T and P. stellifer DSM 14472T, which was lower than the recommended threshold value (dDDH?<?70%). The strain HN-1T contain anteiso-C15:0 as major fatty acids and MK-7 as predominant isoprenoid quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four aminophospholipids and an unidentified glycolipid. Unlike the most closely related P. stellifer DSM 14472T, strain HN-1T or 39 was positive for catalase reaction. Distinct phenotypic and genomic characterisations from previously described taxa support the classification of strains HN-1T or 39 as representatives of a novel species of the genus Paenibacillus, for which the name Paenibacillus sinensis is proposed, with type strains HN-1T (=CGMCC 1.18902, JCM 34,620), and reference strain 39 (=CGMCC 1.18879, JCM 34,616), respectively. 相似文献
4.
A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83 T, is Gram-positive, aerobic, motile and rod-shaped (0.4–0.6 μm × 1.9–2.6 μm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83 T was a member of the genus Cohnella. High similarity of 16S rRNA gene sequence was found between YN-83 T and Cohnella ginsengisoli DSM18997 T (97.99%), whereas the similarity was below 96.0% between YN-83 T and the other Cohnella species. DNA–DNA relatedness between strain YN-83 T and C. ginsengisoli DSM18997 T was 27.4 ± 6.2%. The DNA G+C content of strain YN-83 T was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C 15:0 (44.3%), iso-C 15:0 (11.3%), iso-C 16:0 (18.6%) and C 16:0 (7.7%). The polar lipids of strain YN-83 T consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA–DNA hybridization, strain YN-83 T represents a novel species of the genus Cohnella, for which the name Cohnella plantaginis sp. nov. (type strain YN-83 T = DSM 25424 T = CGMCC 1.12047 T) is proposed. 相似文献
5.
A nitrogen-fixing bacterium, designated strain gs65 T, was isolated from a rhizosphere soil sample of Caragana kansuensis Pojark. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain gs65 T is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarity were found between strain gs65 T and Paenibacillus borealis DSM 13188 T (97.5 %), Paenibacillus odorifer ATCC BAA-93 T (97.3 %), Paenibacillus durus DSM 1735 T (97.0 %) and Paenibacillus sophorae DSM23020 T (96.9 %). Levels of 16S rRNA gene sequence similarity between strain gs65 T and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA–DNA relatedness between strain gs65 T and P. borealis DSM 13188 T, P. odorifer ATCC BAA-93 T (97.3 %), P. durus DSM 1735 T and P. sophorae DSM23020 T were 35.9, 38.0, 34.2 and 35.5 % respectively. The DNA G+C content of strain gs65 T was determined to be 51.6 mol%. The major fatty acids were found to be iso-C 14:0, anteiso-C 15:0 and iso-C 16:0. On the basis of its phenotypic characteristics and levels of DNA–DNA hybridization, strain gs65 T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus taohuashanense sp. nov. is proposed. The type strain is gs65 T (=CGMCC 1.12175 T = DSM 25809 T). 相似文献
6.
A Gram-positive, nitrogen-fixing and endospore-forming strain, designated P121T, was isolated from the gut of the armored catfish (Parotocinclus maculicauda) and identified as a member of the genus Paenibacillus based on the sequences of the 16S rRNA encoding gene, rpoB, gyrB and nifH genes and phenotypic analyses. The most closely related species to strain P121T were Paenibacillus rhizoplanae DSM 103993T, Paenibacillus silagei DSM 101953T and Paenibacillus borealis DSM 13188T, with similarity values of 98.9, 98.3 and 97.6%, respectively, based on 16S rRNA gene sequences. Genome sequencing revealed a genome size of 7,513,698 bp, DNA G?+?C content of 53.9 mol% and the presence of the structural nitrogenase encoding genes (nifK, nifD and nifH) and of other nif genes necessary for nitrogen fixation. Digital DNA-DNA hybridization (dDDH) experiments and average nucleotide identity (ANI) analyses between strain P121T and the type strains of the closest species demonstrated that the highest values were below the thresholds of 70% dDDH (42.3% with P. borealis) and 95% ANI (84.28% with P. silagei) for bacterial species delineation, indicating that strain P121T represents a distinct species. Its major cellular fatty acid was anteiso-C15:0 (42.4%), and the major isoprenoid quinone was MK-7. Based on physiological, genomic, biochemical and chemotaxonomic characteristics, we propose that strain P121T represents a novel species for which the name Paenibacillus piscarius sp. nov. is proposed (type strain?=?DSM 25072?=?LFB-Fiocruz 1636). 相似文献
7.
Strain DY6 T, a Gram-positive endospore-forming motile rod-shaped bacterium, was isolated from soil in South Korea and characterized to determine its taxonomic position. Phylogenetic analyses based on the 16S rRNA gene sequence of strain DY6 T revealed that strain DY6 T belongs to the genus Paenibacillus in the family Paenibacillaceae in the class Bacilli. The highest degree of sequence similarities of strain DY6 T were found with Paenibacillus gansuensis B518 T (97.9%), P. chitinolyticus IFO 15660 T (95.3%), P. chinjuensis WN9T (94.7%), and P. rigui WPCB173 T (94.7%). Chemotaxonomic data revealed that the predominant fatty acids were anteiso-C 15:0 (38.7%) and C 16:0 (18.0%). A complex polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The predominant respiratory quinone was MK-7. Based on these phylogenetic, chemotaxonomic, and phenotypic data, strain DY6 T (=KCTC 33026 T =JCM 18491 T) should be classified as a type strain of a novel species, for which the name Paenibacillus swuensis sp. nov. is proposed. 相似文献
8.
A Gram-positive and endospore-forming strain, JH8T, was isolated from deep-sea sediment and identified as a member of the genus Paenibacillus on the basis of 16S rRNA gene sequence and phenotypic analyses. According to a phylogenetic analysis, the most closely related species was Paenibacillus wynnii LMG 22176T (96.9%). Strain JH8T was also facultatively anaerobic and grew optimally at 20-25degreesC. The major cellular fatty acid was anteiso-C15:0, and the DNA G+C content was 53.1 mol%. The DNA-DNA relatedness between the isolate and Paenibacillus wynnii LMG 22176T was 7.6%, indicating that strain JH8T and P. wynnii belong to different species. Based on the phylogenetic, phenotypic, and chemotaxonomic characteristics, strain JH8T would appear to belong to a novel species, for which the name Paenibacillus donghaensis sp. nov. is proposed (type strain =KCTC 13049T=LMG 23780T). 相似文献
11.
A Gram-stain positive, facultative anaerobic endospore-forming bacterium, designated strain YIM h-19 T, was isolated from a tobacco sample. Cells were observed to be motile rods by means of peritrichous flagella and colonies were observed to be convex, yellow, circular and showed catalase-positive and oxidase-negative reactions. Strain YIM h-19 T was able to grow at 4–45 °C, pH 6.0–8.0 and 0–3 % NaCl (w/v). The predominant respiratory quinone was identified as MK-7. Major fatty acids were identified as anteiso-C 15:0, anteiso-C 17:0 and C 16:0. The polar lipids were found to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. The genomic DNA G+C content was determined to be 54 mol%. 16S rRNA gene sequence analysis showed the strain YIM h-19 T was most closely related to Paenibacillus hordei RH-N24 T and Paenibacillus hunanensis FeL05 T with similarities of 98.30 and 94.64 % respectively. However, DNA–DNA hybridization data indicated that the isolate represented a novel genomic species with the genus Paenibacillus. All data from genotypic and phenotypic analyses support the conclusion that strain YIM h-19 T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus nicotianae sp. nov. is proposed. The type strain is YIM h-19 T (=CGMCC1.12819 T = NRRL B-59112 T). 相似文献
12.
Strain CO 4–7 T was isolated from greenhouse soil used for cultivation of cucumbers in Korea. The 16S rRNA gene sequence of strain CO 4–7 T showed the highest sequence similarity with Paenibacillus contaminans CKOBP-6 T (94.2%) among the type strains. Strain CO 4–7 T was a strictly aerobic, Gram-staining-positive, endospore-forming, and motile rodshaped bacterium. Strain CO 4–7 T grew at 10–45°C (optimum, 30°C), at pH 6.0–7.5 (optimum, pH 6.5) and in the presence of 0–5% NaCl (optimum, 0.5%). The DNA G+C content of strain CO 4–7 T was 48.5 mol%. It contained MK-7 as the major isoprenoid quinone and anteiso-C15:0 (51.8%), C16:0 (12.7%), and iso-C16:0 (8.6%) as the major fatty acids. The cell wall contained meso-diaminopimelic acid. Based on evidence from our polyphasic taxonomic study, it was concluded that strain CO 4-7T should be classified as a novel species of the genus Paenibacillus, for which, the name Paenibacillus cucumis sp. nov. is proposed. The type strain is CO 4–7 T (=KACC 17444 T=JCM 19515 T). 相似文献
15.
A novel actinomycete, designated strain NEAU-NH11 T, was isolated from muddy soil collected from a lake and characterized using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-NH11 T belongs to the genus Streptosporangium, and was most closely related to Streptosporangium amethystogenes subsp. amethystogenes DSM 43179 T (99.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-NH11 T formed a monophyletic clade with Streptosporangium purpuratum CY-15110 T (98.3 %) and Streptosporangium yunnanense CY-11007 T (98.0 %), an association that was supported by a bootstrap value of 80 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from S. amethystogenes subsp. amethystogenes DSM 43179 T, S. purpuratum CY-15110 T and S. yunnanense CY-11007 T. Moreover, strain NEAU-NH11 T could also be differentiated from its closest related strains by phenotypic characteristics. Therefore, it is proposed that strain NEAU-NH11 T represents a novel Streptosporangium species, Streptosporangium nanhuense sp. nov. The type strain of S. nanhuense is NEAU-NH11 T. (=CGMCC 4.7131 T = DSM 46674 T). 相似文献
17.
A Gram-negative, nonmotile, endospore-forming, rod-shaped bacterial strain LHW35 T, which belonged to the genus Paenibacillus, was isolated from marine sediment collected from the south coast of the Republic of Korea. A phylogenetic analysis of 16S rRNA gene sequences indicated that strain LHW35 T was most closely related to Paenibacillus taiwanensis G-soil-2-3 T (97.2% similarity). The optimal growth conditions for strain LHW35 T were 37°C, pH 6.0, and 0% (w/v) NaCl. The main isoprenoid quinone was menaquinone-7 (MK-7) and the major polyamine was spermidine. The diamino acid present in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major fatty acids were anteiso-C 15:0 and C 16:0. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified amino-hospholipids, unidentified phospholipids, and unidentified polar lipids. A DNA-DNA hybridization experiment using the type strain of P. taiwanensis indicated <40% relatedness. The DNA G+C content was 45.0 mol%. Based on these phylogenetic, genomic, and phenotypic analyses, strain LHW35 T should be classified as a novel species within the genus P aenibacillus, for which the name Paenibacillus marinisediminis sp. nov. is proposed. The type strain is LHW35 T (=KACC 16317 T =JCM 17886 T). 相似文献
19.
A Gram-positive, yellow pigmented strain, BKS 3-46 T was isolated from a soil sample collected from the rhizosphere of Ficus benghalensis (banyan tree) in Bhitarkanika mangrove forest, in the Indian state of Odisha, and subjected to polyphasic taxonomic study. The 16S rRNA gene sequence of the strain was determined and the sequence analysis showed that strain BKS 3-46 T should be assigned to the genus Isoptericola. The chemotaxonomic data supported this taxonomic placement i.e. presence of menaquinone MK-9(H 4); major fatty acids anteiso C 15:0 and iso-C 15:0; and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol (PI) as major polar lipids. Further phylogenetic analysis of the 16S rRNA gene sequence confirmed that the strain BKS 3-46 T belongs to the genus Isoptericola and is closely related to Isoptericola halotolerans MTCC 11265 T (98.6 %) followed by Isoptericola nanjingensis MTCC 11633 T (98.4 %) and Isoptericola chiayiensis MTCC 11634 T (98.1 %). However, the DNA–DNA hybridization values obtained between strain BKS 3-46 T and other related strains were well below the threshold that is required for the proposal of a novel species. The G+C content of the genomic DNA was determined to be 70.4 mol%. The phenotypic and genotypic data showed that the strain BKS 3-46 T merits the recognition as a representative of a novel species of the genus Isoptericola. It is proposed that the isolate should be classified in the genus Isoptericola as a novel species, Isoptericola rhizophila sp. nov. The type strain is BKS 3-46 T (=MTCC 11080 T=JCM 19252 T). 相似文献
20.
Antonie van Leeuwenhoek - A novel protease-producing actinobacterium, designated strain NEAU-A11T, was isolated from soil collected from Aohan banner, Chifeng, Inner Mongolia Autonomous Region,... 相似文献
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