Miscellaneous notes on Discomycetes of the Bonin Islands,Ani-jima Island

Cup fungi of Ani-jima Island, the Bonin Islands, were collected in November 1990 and described for the first time from Ani-jima Island. Four species,Dicephalospora rufocornea, Lachnum abnormis, Lachnum pritzelianum, andOrbilia delicatula were collected from the materials in the litter layer ofLivistona chinensis var.boninensis community around Mt. Kita-hutago in Ani-jima Island.Pulvinula globifera was collected from the soil under a camellia (Schima mertensiana) community to the southeast of Mt. Kita-hutago.Lachnum pritzelianum was new to Japan.     

Two new species ofMarasmius from eastern Honshu, Japan

Two new species belonging toMarasmius (Agaricales) are described and illustrated from eastern Honshu, Japan:Marasmius capitatus sp. nov. (sectionEpiphylli), forming capitate cystidia and small basidiomata with a membranous, white pileus and a minutely pubescent, filiform stipe without basal mycelium, was found on dead fallen leaves ofCryptomeria japonica; Marasmius nivicola sp. nov. (sectionGlobulares), having entirely white, collybioid basidiomata with a furfuraceous to pruinose stipe and distinctly intervenose lamellae, was found on leaf litter of broad-leaved forest.     

Five new species of the Boletaceae from Japan

Five new species of the Boletaceae (Agaricales) from Japan are described and illustrated: (1) Boletus bannaensis sp. nov. (section Luridi), forming a grayish-brown pileus and rufescent flesh, found in subtropical evergreen broad-leaved forests; (2) Leccinum rhodoporosum sp. nov., forming discolorous red pores, a whitish stipe covered overall with violet-brown to blackish-brown furfuraceous scales and fusoid-cylindrical brown basidiospores, found in subtropical evergreen broad-leaved forests or warm-temperate Quercus-Pinus forests; (3) Pulveroboletus brunneoscabrosus sp. nov., forming a lemon-yellow pulverulent basidiomata covered overall with orange to brownish-orange appressed scales, found in subtropical to warm temperate evergreen broad-leaved forests; (4) Rubinoboletus monstrosus sp. nov., forming a brownish-orange to yellowish-brown pileus and a very short, nonreticulate, hollow stipe, found in subtropical evergreen broad-leaved forests; and (5) Tylopilus fuligineoviolaceus sp. nov., having a deep violet to blackish-brown pileus and brunnescent hymenophore, found in warm temperate Quercus-Castanopsis forests.     

Two new species of Agaricales from southwestern islands of Japan

Two new species of Agaricales are described and illustrated from Yaeyama Islands, southwestern Japan: (1) Amanita rubromarginata sp. nov. (section Caesareae Sing. ex Sing. in the subgenus Amanita), forming brownish-orange then reddish-yellow pileus, a pale yellow, squamulose stipe with a thin, membranous, reddish-orange annulus and a thick, saccate volva, and reddish-orange marginate lamellae, found in oak forests of Ishigaki Island; and (2) Tylopilus obscureviolaceus sp. nov. (section Tylopilus), having dark purple basidiomata, white, bitter, unchanging flesh, and a finely purplish-reticulate stipe, found in oak forests of Iriomote Island.     

Growth and reproductive patterns of <Emphasis Type="Italic">Undaria pinnatifida</Emphasis> sporophytes in a cultivation farm in Busan,Korea

Monthly growth and reproduction of Undaria pinnatifida sporophytes were examined over a period of 5 months in a cultivation farm in Korea. A total of 11 characters of Undaria were measured to determine a reliable morphological character representing its growth and reproduction. Plant weight of Undaria sporophytes increased steadily over the experimental period, but it increased in four different ways. Undaria pinnatifida increased body weight by growth in length and width (October–early December), and by growth in width with the thickening of blade and stipe when sporophytes began to be fertile (December–January). In the middle of January, growth in length and width had almost stopped with the maturation of Undaria sporophytes. Finally, the weight of Undaria increased again by growth in width at the end of February. Present results indicate that Undaria sporophytes increase body weight by growth in length and width at different times, and the relationship between reproduction and vegetative growth is exclusive. Plant weight was positively correlated and fitted well with stipe width and blade width. The blade of Undaria was very thin (ca. 254 μm) and breakable by wave action, but its stipe was strong and relatively thick (ca. 8.7 mm). Furthermore, the fertility of U. pinnatifida was fitted better with stipe width than blade width. Thus, we suggest that the stipe width is the most feasible character with which to estimate the growth and reproduction of U. pinnatifida sporophytes in the cultivation farm.     

A new variety ofCortinarius elatior from Japan

Cortinarius elatior var.albipes var. nov., found in the deciduous forest is described and illustrated. It is distinguished from the type variety by having a white stipe and longer spores. The differences betweenCortinarius elatior var.albipes and similar taxa are briefly discussed.     

羊肚菌烂柄病发生对土壤真菌群落结构的影响

为了解羊肚菌(Morel)烂柄病的发生对土壤真菌群落结构的影响,采用Illumina MiSeq高通量测序技术,对健康羊肚菌根际土、烂柄病发病子实体根际土及相同环境下未栽培羊肚菌土壤的真菌群落结构进行研究。结果表明,测序样品共获得344 163条序列,归为7个真菌门。各样品真菌多样性分析结果表明,未栽培土壤真菌群落多样性较高,栽培羊肚菌根际土壤真菌多样性显著降低,烂柄病发生后土壤真菌多样性增加。群落结构分析表明,烂柄病根际优势真菌类群为拟青霉属(Paecilomyces)、木霉属(Trichoderma)、葡萄穗霉属(Stachybotrys)、枝顶孢属(Acremonium)、Paratritirachium、Zopfiella、被孢霉属(Mortierella)和柄孢壳属(Podospora)。烂柄病的发生改变了土壤真菌群落结构,促进了根际真菌的繁殖。为了解羊肚菌烂柄病的发生、传播机理及防治提供了参考。     

Seasonal variations in sterol composition of Delesseria sanguinea (Ceramiales,Rhodophyta)

On Normandy coasts, the red alga Delesseria sanguinea perennates by its stipe; fronds grow in January and disappear in June. Seasonal variations in sterol composition in relation to the biology of D. sanguinea are reported. Sterols in cellular membranes are free or conjugated by esterification with fatty acids, heterosides or lipid complexes like phospholipids. Both kinds of sterols were analyzed by GC-MS. The major sterol (80%) found in fronds was cholesterol whereas in stipes, cholesterol was also the major sterol in spring, but in September, an important reduction in cholesterol yield was noted with proportional increase in sitosterol content. It appears that cholesterol is synthesized in fronds in spring, then transferred to the stipe, which loses an important amount of cholesterol with loss of the blades.     

Influence of light on the morphological changes that take place during the development of the <Emphasis Type="Italic">Flammulina velutipes</Emphasis> fruit body

We show that fruit bodies of Flammulina velutipes can be induced in complete darkness after a sharp temperature reduction (23° to 16°C). However, the fruit bodies that form in complete darkness have a long stipe with an undeveloped pileus on the top (pinhead fruit bodies) and are thinner and whiter than the normal fruit bodies which are formed in the light. This finding suggests that F. velutipes fruit bodies cannot mature in complete darkness. However, when we irradiated the fruit bodies that had formed in complete darkness, a pileus developed immediately, and 4 days later the separation between the stipe and the pileus could be observed. Immediately after light exposure, the stipe also thickened and became increasingly pigmented. The stipe elongation was inhibited until 8 days after light exposure, although stipe elongation progressed very quickly thereafter. Basidospores were also visible in the gills 8 days after light exposure. We consider that the basidiospore development is involved in this rapid stipe elongation, which aids the effective dispersal of basidiospores.     

<Emphasis Type="Italic">Strobilomyces verruculosus</Emphasis> sp. nov. from Japan

A new species, Strobilomyces verruculosus, is described and illustrated. It is morphologically distinct from other species of Strobilomyces by having a verruculose pileus with small subpyramidal scales, a long and thick stipe with small warty to appressed scales, subdecurrent tubes, and incompletely reticulate basidiospores. Molecular phylogenetic analyses using the mitochondrial cytochrome oxidase subunit 3 (cox3) gene support that it belongs to the genus Strobilomyces and is highly differentiated from the other members of this genus found in Japan.     

Three new species ofMarasmius sectionSicci from eastern Honshu, Japan

Three new species belonging toMarasmius sectionSicci (Agaricales) are described and illustrated from eastern Honshu, Japan:Marasmius nocturnus sp. nov., forming a dark brown pileus and marginate lamellae, was found on leaf litter inPasania—Quercus forests;Marasmius occultatus sp. nov., producing brownish orange or light brown basidiomata and long, cylindrical-fusoid basidiospores, was found on dead fallen twigs ofAphananthe aspera andQuercus myrsinaefolia;Marasmius opulentus sp. nov., having a reddish orange pileus and a pubescent stipe, was found on leaf litter in laurel-leaved forest.     

Variations in the alginate content and composition of Durvillaea antarctica and D. willana from southern New Zealand

The brown seaweeds Durvillaea antarctica andD. willana are dominant components of the lowerlittoral and upper sublittoral of exposed rocky shoresin southern New Zealand. Tissue samples of bothspecies, harvested from a site on the south-east coastof South Island over a period of 2 years, wereanalysed for alginate content and composition.Individuals of both species were further separatedinto different blade (lamina and palm) and stipe(cortex and medulla) fractions to assess variationwithin the thallus. On average the alginate contentand frequency of mannuronic acid (F_m) was higherin D. antarctica than in D. willana. Blades contained more alginate than stipes, laminaeand stipes were rich in mannuronic acid whereasholdfasts were rich in guluronic acid. Variations incomposition are considered to reflect the functionaldifferences of the tissue, giving flexibility to bladeand stipe and rigidity to the holdfast. Despitefluctuations in content and composition betweencollection times no seasonal trends in eithercomponent were apparent.     

Gymnopus piceipes sp. nov. from Japan

Gymnopus piceipes is proposed as a new species within the sectionVestipedes. It is characterized by a brown pileus, pale brown close lamellae, a solid black stipe that is not insititious, and conspicuous pleurocystidia.     

Microscopic observations on tissue calcium distribution in the brown alga,Undaria pinnatifida (Harvey) Suringar

The distribution of Ca²⁺ in thallus tissues of the brown algaUndaria pinnatifida was investigated by microspectro-photometric analysis after fixation with Carnoy's reagent and staining with an alizarine sulfonate dye. Ca²⁺ accumulation in the young thallus was much higher in mucilage cells of the basal than the apical region; there was least in the pith of the basal region. A roughly uniform pattern was found in the stipe from the central part of the young thallus, while a highly irregular pattern occurred in both stipe and blade from the corresponding area of the mature thallus; the Ca²⁺ content was much lower than in the cortex, mucilage cells and vacuoles of the mature stipe than in the corresponding area of the young stipe. The contents of all regions of the mature blade were higher than those in the young blade; they were particularly high in mucilage cells.     

Three new species ofCrinipellis found in Iriomote Island, southwestern Japan, and central Honshu, Japan

Three new species ofCrinipellis (Agaricales, Basidiomycetes) in Japan are described and illustrated:Crinipellis canescens sp. nov., forming small, canescent basidiomata, was found on a dead fallen twig in the subtropical laurelleaved forest of Iriomote Island (southwestern Japan);Crinipellis corvina sp. nov., forming fibrillose-strigose, puriplish black hairs enveloping the whole basidioma, was found on bark ofTorreya nucifera in Mt. Takao, Tokyo;Crinipellis nigricaulis sp. nov., having a reddish brown pileus with a minute, black papilla and a blackish stipe accompanied by blackish, branched rhizomorphs, was found on leaf litter in the lowland forest of Kanagawa.     

Notes on the genus <Emphasis Type="Italic">Inocybe</Emphasis> of Japan. IV. Species having metuloids collected from Hokkaido,Honshu, and Kyushu

This fourth paper in the series considers five species of Inocybe occurring in Hokkaido, Honshu, and Kyushu. (1) Inocybe furfurea (section Tardae) is recorded from Hokkaido as new to Japan. (2) Inocybe luteola sp. nov. (section Tardae) is described from eastern Honshu (Chiba). It has smooth basidiospores and thick-walled caulocystidia descending to the middle of the stipe. (3) Inocybe napiformis sp. nov. [section Inocybe (= Cortinatae)], known from Hokkaido, appears close to Inocybe napipes, but characters of the metuloids distinguish the two species. (4) Inocybe grammata (section Marginatae) is recorded from Hokkaido and Nagano as new to Japan. (5) Inocybe pyriformis sp. nov. (section Marginatae) is described from Kyushu (Miyazaki). It has nodulose basidiospores and caulocystidia wholly covering the stipe surface. However, I. pyriformis is not typical in the section, lacking a marginate bulbous base in its stipe.     

角唇隔距兰,中国隔距兰属(兰科)一新记录种

报道了中国兰科(Orchidaceae)隔距兰属(Cleisostoma Blume)一新记录种:角唇隔距兰(C.tricornutum Averyanov)。该种与C.crochetii(Guillaumin)Garay、C.parishii(J.D.Hooker)Garay相近,因其有不分枝的线状花粉团柄、球状的花粉团块和一个先端短喙状的半球形药帽而与C.crochetii(Guillaumin)Garay相区别;因其有松散、罕分枝的花序,长角状的唇瓣侧裂片而与C.parishii(J.D.Hooker)Garay相区别。提供了新记录种的形态描述及图片。     

A new species of <Emphasis Type="Italic">Gymnopilus</Emphasis> Sect. <Emphasis Type="Italic">Microspori</Emphasis> from Japan

Gymnopilus ombrophilus sp. nov., growing on rotten wood of conifers and hardwoods from Niigata, Japan is described and illustrated. It is characterized by its medium-sized, brownish-orange basidiocarps with a finely squamulose pileus, stipe lacking an annulus, and mild taste, and microscopically by dextrinoid, small, broadly ellipsoid basidiospores. The new species belongs to the section Microspori. The differences between the taxon and similar species are briefly discussed.     

Two new species ofMarasmiellus from eastern Honshu, Japan

Two new species ofMarasmiellus are described from eastern Honshu, Japan:Marasmiellus atrostipitatus sp. nov. (sectionRameales subsectionOpacini) is characterized by a white pileus and a blackish stipe covered with white pruinose to flocculos scales.Marasmiellus brunneocarpus sp. nov. (sectionTricolores) has small brownish basidiomata. Both species occur on dead leaves and twigs inQuercus-Eurya forests.