共查询到20条相似文献,搜索用时 46 毫秒
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M. Salemi A.E. Calogero G. Zaccarello R. Castiglione A. Cosentino M. Catanuso E. Vicari G. Rappazzo 《European journal of histochemistry : EJH》2010,54(3)
The sperm protein associated with the nucleus in the X chromosome (SPANX) gene family encodes for proteins that are not only expressed in germ cells, but also in a number of tumors. In addition, SPANX genes map in an interval of the X chromosome (namely, Xq27), which has been found to be associated with familial prostate cancer by linkage analysis. The aim of this study was therefore to evaluate SPANX protein expression in normal prostate tissues and in prostate carcinoma. For this purpose, formalin-fixed and paraffin-embedded sections obtained from 15 normal (at autopsy) donors and 12 men with prostate cancer were analyzed by immunohistochemistry. About 40% of both normal and tumor prostate samples resulted SPANX positive. Signals were exclusively within the nucleus in normal prostate cells, whereas both nuclear and cytoplasmic positivity was observed in tumor cells. In conclusion, these findings showed that SPANX genes are expressed in both normal and tumor prostate gland, but the latter showed a peculiar cytoplasmic staining positivity. This suggests a possible association between SPANX over expression and prostate cancer development. Additional studies are needed to corroborate this hypothesis.Key words: cancer, prostate, immunohistochemistry, SPANX gene 相似文献
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Deanna Acosta Masako Suzuki Diana Connolly Reid F. Thompson Melissa J. Fazzari John M. Greally Cristina Montagna 《Mammalian genome》2011,22(3-4):249-259
Epigenetic inactivation due to aberrant promoter methylation is a key process in breast tumorigenesis. Murine models for human breast cancer have been established for nearly every important human oncogene or tumor suppressor gene. Mouse-to-human comparative gene expression and cytogenetic profiling have been widely investigated for these models; however, little is known about the conservation of epigenetic alterations during tumorigenesis. To determine if this key process in human breast tumorigenesis is also mirrored in a murine breast cancer model, we mapped cytosine methylation changes in primary adenocarcinomas and paired lung metastases derived from the polyomavirus middle T antigen mouse model. Global changes in methylcytosine levels were observed in all tumors when compared to the normal mammary gland. Aberrant methylation and associated gene silencing was observed for Hoxa7, a gene that is differentially methylated in human breast tumors, and Gata2, a novel candidate gene. Analysis of HOXA7 and GATA2 expression in a bank of human primary tumors confirms that the expression of these genes is also reduced in human breast cancer. In addition, HOXA7 hypermethylation is observed in breast cancer tissues when compared to adjacent tumor-free tissue. Based on these studies, we present a model in which comparative epigenetic techniques can be used to identify novel candidate genes important for human breast tumorigenesis, in both primary and metastatic tumors. 相似文献
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Craig P. Giacomini Steven Sun Sushama Varma A. Hunter Shain Marilyn M. Giacomini Jay Balagtas Robert T. Sweeney Everett Lai Catherine A. Del Vecchio Andrew D. Forster Nicole Clarke Kelli D. Montgomery Shirley Zhu Albert J. Wong Matt van de Rijn Robert B. West Jonathan R. Pollack 《PLoS genetics》2013,9(4)
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Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice 总被引:1,自引:0,他引:1 下载免费PDF全文
Huang S Li Y Chen Y Podsypanina K Chamorro M Olshen AB Desai KV Tann A Petersen D Green JE Varmus HE 《Genome biology》2005,6(10):R84-13