共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
The 5'-terminal ribonucleotide of an in vitro ColE1 transcript is not complementary to the DNA template 总被引:3,自引:0,他引:3
R C Parker 《Gene》1983,26(2-3):127-136
13.
14.
15.
M Carey J Kolman D A Katz L Gradoville L Barberis G Miller 《Journal of virology》1992,66(8):4803-4813
16.
17.
18.
Kevin J. Travers Chen-Shan Chin David R. Rank John S. Eid Stephen W. Turner 《Nucleic acids research》2010,38(15):e159
A novel template design for single-molecule sequencing is introduced, a structure we refer to as a SMRTbell™ template. This structure consists of a double-stranded portion, containing the insert of interest, and a single-stranded hairpin loop on either end, which provides a site for primer binding. Structurally, this format resembles a linear double-stranded molecule, and yet it is topologically circular. When placed into a single-molecule sequencing reaction, the SMRTbell template format enables a consensus sequence to be obtained from multiple passes on a single molecule. Furthermore, this consensus sequence is obtained from both the sense and antisense strands of the insert region. In this article, we present a universal method for constructing these templates, as well as an application of their use. We demonstrate the generation of high-quality consensus accuracy from single molecules, as well as the use of SMRTbell templates in the identification of rare sequence variants. 相似文献
19.
20.