The indirect effect of hemoglobin on the electrophoretic mobility of human blood erythrocytes

Using the factor analysis, it was shown that the total content of hemoglobin in human blood plays a limited and indirect role in the regulation of average electrophoretic mobility of erythrocytes. In this case, it is not the only parameter governing this level. The statistical relationship between the total content of hemoglobin and erythrocyte mobility in electrical field is not stable and is determined by the dependence of both indices on their common factor of control of erythroid homeostasis.     

Effect of procaine hydrochloride on the electrophoretic mobility of human red blood cells

The interaction of cationic anesthetics with biological membranes and the resulting alterations of membrane electrokinetic properties continue to be of current interest. The present study was designed to examine the effects of procaine hydrochloride (PRHCL) on the mobility of human red blood cells (RBC); electrophoretic measurements were made on RBC suspended in phosphate-buffered saline (PBS, pH = 5.0, 7.4, or 9.2), autologous plasma or 3 g% dextran T70/PBS (pH = 7.4), with PRHCL concentrations from 8 x 10(-6) to 8 x 10(-2) M. Low concentrations of PRHCL (8 x 10(-5)-8 x 10(-3) M) significantly (p less than 0.001) increased RBC mobility, with a maximal increase of 8.2% at 8 x 10(-4) M. Conversely, a higher PRHCL concentration (8 x 10(-2) M significantly (p less than 0.001) decreased RBC mobility. Both glutaraldehyde fixation and lipid extraction abolished any PRHCL-induced increase in RBC mobility; the observed increases in mobility for normal cells are, thus, consistent with a mechanism based on expansion of the RBC membrane glycocalyx. Microelectrophoretic methods were also used to study the effect of PRHCL (8 x 10(-4) and 8 x 10(-2) M) on RBC membrane calcium binding, with the results indicating that PRHCL competes with calcium for neuraminate binding sites. We conclude that the observed changes in RBC electrokinetic properties reflect incorporation of PRHCL into the RBC membrane; such changes may be of importance in modulating cell-cell interactions.     

The effect of immune complexes on the electrophoretic mobility of sheep erythrocytes

Influence of concentration of aggregated immunoglobulins with varying molecular weights on the electrophoretic mobility of ram erythrocytes has been explored. It was shown, that electrophoretic mobility of ram erythrocytes depend on the quantity and the size of adsorption complexes.     

The influence of unipolar air ions on the electrophoretic mobility of erythrocytes

Positive air ions decreased the electrophoretic mobility of erythrocytes of cats while negative air ions increased it. Inhalation of normal air supplied by a fan did not affect the electrophoretic mobility of erythrocytes.

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Zusammenfassung Positive Luftionen setzten die elektrophoretische Beweglichkeit der Erythrocyten von Katzen herab, während negative Luftionen sie steigerten. Die Inhalation normaler Luft, die von einem Ventilator unterstüzt wurde,beeinflusste die elektrophoretische Beweglichkeit von Erythrocyten nicht.

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Resume Les ions positifs diminuent la mobilité électrophorétique des érythrocytes des chats, alors que les ions négatifs l'augmentent. L'inhalation d'air normal,même activée par un ventilateur, ne l'influence par contre pas.

     

Effect of physiological parameters of blood on magnetophoretic mobility of erythrocytes

Magnetophoresis is the process of the particle motion under the influence of a magnetic field. The magnetic particle and medium are considered responsive to the imposed magnetic field, and the material property that describes the response to the external magnetic field is relative magnetic permeability, and the magnetic susceptibility. The present work aims to evaluate the effect of internal and external physiological parameters on the erythrocytes' magnetophoretic mobility (MM) using Cell Tracking Velocimetry (CTV). The results of this study showed that there are a strong correlation between MM and several physiological blood parameters such as mean corpuscle hemoglobin (MCH), red blood cells distribution width (RDW), mean corpuscle hemoglobin concentration (MCHC), and fibrinogen.     

Effect of physiological parameters of blood on magnetophoretic mobility of erythrocytes

Magnetophoresis is the process of the particle motion under the influence of a magnetic field. The magnetic particle and medium are considered responsive to the imposed magnetic field, and the material property that describes the response to the external magnetic field is relative magnetic permeability, and the magnetic susceptibility. The present work aims to evaluate the effect of internal and external physiological parameters on the erythrocytes' magnetophoretic mobility (MM) using Cell Tracking Velocimetry (CTV). The results of this study showed that there are a strong correlation between MM and several physiological blood parameters such as mean corpuscle hemoglobin (MCH), red blood cells distribution width (RDW), mean corpuscle hemoglobin concentration (MCHC), and fibrinogen.     

Seasonal activity of frog erythrocytes by data of electrophoretic mobility

The peculiarities of frog erythrocyte electrophoretic mobility, coupled to the seasonal course of temperatures, have been studied. At the periods of anabiosis and of burst of hemopoiesis, in the vascular bed there increases the portion of functionally young erythrocytes (up to 22%) with increased values of the cell membrane surface charge. Preparation to winter is accompanied by a rise of the number of circulating functionally worn-down blood cells (up to 60%) with low values of the superficial charge and low mobility in electrical field. Use of the cell microelectrophoresis method of evaluation of seasonal activity of frog erythrocytes allows obtaining objective data about the cellular surface charge and its depending functional cell activity without submitting the erythrocytes to modifying actions.     

Effect of oxidized glutathione on some enzymes of erythrocytes and its relation to erythrocytic enzyme activity and electrophoretic mobility

1. Oxidized glutathione reacts or interacts with some erythrocytic enzymes (glucose 6-phosphate dehydrogenase, EC 1.1.1.49, aspartate aminotransferase, EC 2.6.1.10) but not with some others (lactate dehydrogenase, EC 1.1.1.27). 2. GSSG does not diminish the activity of any of these enzymes and is therefore not responsible for the decreased enzyme activities associated with older erythrocytes. 3. It may be that the reaction of aspartate aminotransferase with GSSG is the cause for the more rapid anodic electrophoretic mobility of this enzyme derived from human erythrocytes when compared with the mobility of the same enzyme from other human tissues. 4. A reinterpretation of some related, previously published, data with regard to the electrophoretic mobility of the above-mentioned enzymes from young and old erythrocytes is presented.     

Effect of the species origin of erythrocytes and of the methods for their subsequent handling on their electrophoretic mobility in producing diagnostic agents

Changes in electrophoretic motility correlate with the effect produced by different reagents used in the production of erythrocyte reagents. This characteristic may be one of the criteria for the evaluation of the quality of erythrocytes in the development of diagnosticums.     

Changes in the electrophoretic mobility of erythrocytes under the action of low-intensity pulsed magnetic field

Exposure of intact rats and human erythrocytes to low-intensity pulsed magnetic field leads to similar biphasic changes in the electrophoretic mobility of erythrocytes; this is accompanied by modification of their membrane and cytoskeletal protein spectrum.     

Effect of neuraminidase and proteolytic enzymes on electrophoretic mobility of erythrocytes and their aggregation induced by La(3+)

The effect of neuraminidase, trypsin and pronase on the electrophoretic mobility of human erythrocytes and their aggregation induced by La3+ was studied in vitro. The aggregation of intact red cells differs from that of cells treated with neuraminidase and proteolytic enzymes. The above differences are observed in the range of La3+ suspension concentration from 20 to 330 microM. A possible mechanism of aggregation is discussed.     

Changes in the electrophoretic mobility of erythrocytes by the action of low-intensity pulse magnetic field

It has been shown that the exposure of "intact" rats and human blood to low-intensity pulse magnetic field leads to similar changes in the electrophoretic mobility of erythrocytes, which may be connected with the modification of the spectrum of their membrane proteins.