Experimental demonstration of possible cryptic female choice on male tsetse fly genitalia

A possible explanation for one of the most general trends in animal evolution - rapid divergent evolution of animal genitalia - is that male genitalia are used as courtship devices that influence cryptic female choice. But experimental demonstrations of stimulatory effects of male genitalia on female reproductive processes have generally been lacking. Previous studies of female reproductive physiology in the tsetse fly Glossina morsitans suggested that stimulation during copulation triggers ovulation and resistance to remating. In this study we altered the form of two male genital structures that squeeze the female's abdomen rhythmically in G. morsitans centralis and induced, as predicted, cryptic female choice against the male: sperm storage decreased, while female remating increased. Further experiments in which we altered the female sensory abilities at the site contacted by these male structures during copulation, and severely altered or eliminated the stimuli the male received from this portion of his genitalia, suggested that the effects of genital alteration on sperm storage were due to changes in tactile stimuli received by the female, rather than altered male behavior. These data support the hypothesis that sexual selection by cryptic female choice has been responsible for the rapid divergent evolution of male genitalia in Glossina; limitations of this support are discussed. It appears that a complex combination of stimuli trigger female ovulation, sperm storage, and remating, and different stimuli affect different processes in G. morsitans, and that the same processes are controlled differently in G. pallidipes. This puzzling diversity in female triggering mechanisms may be due to the action of sexual selection.     

Effects of Host Resistance on the Fecundity of Globodera rostochiensis

The fecundity of Globodera rostochiensis (R₁A) females that developed on resistant Rosa and susceptible Katahdin potato cultivars were compared. Cysts collected from each cultivar were bulked, separated into four sizes (> 500 μm, 355-500 μm, 250-355 μm, and < 250 μm), and crushed to determine fecundity as measured by viable egg content (VEC). Fewer and generally smaller cysts developed on Rosa than on Katahdin. Although cyst size significantly (P = 0.01) influenced VEC, cyst age (8 or 13 weeks) had no effect. Regardless of size, cysts produced on Rosa contained significantly fewer viable eggs than did cysts produced on Katahdin. The fecundity of progeny from cysts produced on Rosa was significantly reduced compared with that of progeny from cysts produced on Katahdin. After two generations on Katahdin, the VEC of cysts from a population originating from Rosa was significantly less than that of cysts from a population originating from Katahdin, indicating that in the presence of a pure population of G. rostochiensis R₁A, the females that develop on the resistant cultivar Rosa represent a diminished rather than a superior selected population.     

On the gametogenesis and early embryogenesis in some olive tree cultivars

A comparative embryological study on two Spanish cultivars and one growing in Bulgaria of Olea europaea was carried out. The embryo sac develops according to Allium (bisporic)-type. The critical phases during the development of male and female gametophyte that lead to sterility and abortiveness of the embryo sacs and ovules are reported. The embryogenesis follows the Asterad-type. The endosperm passes a free nuclear stage and later on becomes cellular.     

Mitochondria-responsive signaling between egg and central cell controls their coordinated maturation

Recent studies suggest a complex regulatory network in female gametophyte of angiosperm. The cell-cell communication between female gametes was confirmed during their maturation and functional specialization. The mitochondria-responsive signaling may play a critical role in this process. Here, we briefly summarized the recent discussion on this topic and proposed a two-pathway’s mechanism for regulating coordinated development of the female gamete cells.     

Females prefer to associate with males with longer intromittent organs in mosquitofish

Sexual selection is a major force behind the rapid evolution of male genital morphology among species. Most within-species studies have focused on sexual selection on male genital traits owing to events during or after copulation that increase a male''s share of paternity. Very little attention has been given to whether genitalia are visual signals that cause males to vary in their attractiveness to females and are therefore under pre-copulatory sexual selection. Here we show that, on average, female eastern mosquitofish Gambusia holbrooki spent more time in association with males who received only a slight reduction in the length of the intromittent organ (‘gonopodium’) than males that received a greater reduction. This preference was, however, only expressed when females chose between two large males; for small males, there was no effect of genital size on female association time.     

Discovery of the female of Pyrocoelia prolongata in Taiwan (Coleoptera, Lampyridae)

The female of Pyrocoelia prolongata Jeng & Lai, a diurnal lampyrid species from Taiwan, is described for the first time. A single individual was found in a small, shady, dry streambed at the edge of a mixed forest at 2700 m elevation. The individual glowed in darkness and would move its abdomen up and down when disturbed and as a deterring behavior. A key to the females of the species of Pyrocoelia Gorham in Taiwan is provided. The morphology of photogenic organs of females and the function of bioluminescence of day-active species of Pyrocoelia are discussed.