景观生态学在大气污染研究中的应用

快速城市化和工业化进程造成了一系列大气污染问题,亟需在宏观尺度上解析大气污染时空分布规律。景观生态学关注格局与过程耦合,景观"源汇"理论可对应解析大气污染物的源与汇效应,将景观生态学的理论与方法引入大气污染研究中已成为解决当前区域发展与大气污染权衡的有效途径。从景观生态学视角辨识了景观与大气污染物的源汇关系,系统梳理了景观格局与大气污染的定量关系,指出当前景观格局指标仍需进一步完善以表征大气污染时空分布特征,而高质量大气污染物时空数据的缺乏是限制景观格局与大气污染过程耦合分析的重要因素,拓展应用景观"源汇"理论,定量解析景观格局对大气污染的源汇效应,同时进一步研发遥感反演技术,实现大气污染物分布格局的精细刻画,将为区域景观规划提供重要支撑。强化大气污染研究中的景观生态学分析途径,将有助于深化景观生态学格局与过程耦合研究体系,也将为景观可持续管理提供有力的科学支撑。     

Life-cycle assessment of continuous pad-dyeing technology for cotton fabrics

Purpose

China is the largest producer of textile-dyeing products in the world. The production of these materials consumes high amounts of water and energy and results in the discharge of huge amounts of pollutants. This study aimed at evaluating the life-cycle environmental impacts of the textile-dyeing industry and determining the key processes for mitigating life-cycle environmental impacts efficiently and effectively, which will benefit the application of cleaner production technologies.

Methods

A life-cycle assessment was performed according to the ISO 14040 standard series. The system investigated includes the dyeing process and final disposal and the transportation of raw material, energy production, and transportation. The functional unit is 10,000 m of cotton fabric, which weighs 2,000 kg. Our study encompasses three types of data. The data regarding the production process and the major raw materials, necessary energy, and the source of the energy, as well as the emissions of some pollutants, were provided by a textile-dyeing enterprise in Jiangsu Province. The data regarding transport were generated using the GaBi version 4.3 database. Some emission factor data such as those on CO₂, CH₄, and N₂O emissions were obtained from the literature. Resources, energy consumption, and emissions are quantified, and some of the potential environmental effects were evaluated using the CML2001 method built into the GaBi version 4.3 database.

Results and discussion

Scouring and oxygen bleaching, dyeing, stentering and setting, wastewater treatment, and incineration are the key processes in terms of global warming potential, acidification potential, photochemical ozone creation potential, and eutrophication potential. It will therefore be useful to enhance the recycling of water, control the consumption of additives and dyes, and conserve energy as much as possible. Through scenario analysis, we note that motorized shipment should be used instead of shipment by trucks, when conditions permit.

Conclusions

To promote energy conservation and the clean production of continuous pad-dyeing technology for cotton fabrics, other environmental impact categories besides the impact of the water system should be given focus. Additional work can be performed on the following: considering a consumption-based perspective of the entire process, uncertainty in data on life-cycle inventory, the evaluation methodology employed, temporal and spatial variation, the normalized toxicity of dyes and additives, and weighting methods.     

Two-dimensional fluorometry coupled with artificial neural networks: a novel method for on-line monitoring of complex biological processes

The use of two-dimensional scanning fluorometry as an on-line, noninvasive, in situ bioreactor monitoring technique is extended to complex bioprocesses using mixed cultures, with particular attention to biofilm systems. Using the example of spectra subtraction, it is demonstrated that established methods for fluorescence data analysis have a limited capability of utilizing overall fluorometric information. Artificial neural networks (ANNs) are introduced as a novel nonlinear and nonmechanistic technique for interpreting the highly complex fluorescence maps. It is shown that ANNs are able to infer process performance parameters in a pattern recognition approach, based on the entire fluorescence "fingerprint" of the biological system. The studies were carried out using an extractive membrane bioreactor (EMB) for the degradation of chlorinated organic compounds, operating with mixed cultures. Model pollutants em- ployed were 1,2-dichloroethane, 3-chloro-4-methylaniline, and p-toluidine.     

Environmental pollutants and skin cancer

We are increasingly exposed to environmental pollution. Pollutants can be inhaled, ingested or come into contact with the skin depending on the form in which they occur. On metabolization, activation, or accumulation, pollutants can become extremely toxic for the vital organs and this is often related to a strong genotoxic effect. Since the skin acts as a barrier between the organism and the environment, it is frequently directly exposed to pollution. It is very often degraded by polluting agents and acts as an inlet toward other tissues. Numerous studies in man recognize and demonstrate the carcinogenic power of certain pollutants in the digestive and respiratory tracts. The "pollutants" that react most specifically with the skin are: ultraviolet radiation, polycyclic aromatic hydrocarbons (e.g., benzo[a]pyrene), volatile organic compounds (e.g., benzene), heavy metals, and ozone. Ultraviolet radiation, a "physical" pollutant, has been described as being the factor responsible for most skin cancers in man. The genotoxicity of UV light is well documented (type of lesion or mutation, etc.) and its carcinogenic effect is clearly demonstratedin vivo in man. A few epidemiological studies describe the carcinogenicity of certain pollutants such as arsenic or lead on the skin. However, most of the evidence for the role of pollutants in skin cancers comes fromin vivo animal studies or fromin vitro studies (e.g., PAHs). In this report, different studies are presented to illustrate the research strategies developed to investigate the mechanism of action of "chemical" pollutants and their potential role in human skin pathology. All the study models and the associated techniques of investigation are tools for a better understanding and thus more efficient prevention of the deleterious effects caused by the environment. This revised version was published online in August 2006 with corrections to the Cover Date.     

Source-sink landscape theory and its ecological significance

Exploring the relatiouships between landscape pattern and ecological processes is the key topic of landscape ecology,for which,a large number of indices as well as landscape pattern analysis model were developed.However,one problem faced by landscape ecologists is that it is hard to link the landscape indices with a specific ecological process.Linking landscape pattern and ecological processes has become a challenge for landscape ecologists."Source" and "sink" are common concepts used in air pollution research,by which the movement direction and pattern of different pollutants in air can be clearly identified.In fact,for any ecological process,the research can be considered as a balance between the source and the sink in space.Thus,the concepts of "source" and "sink" could be implemented to the research of landscape pattern and ecological processes.In this paper,a theory of sourcesink landscape was proposed,which include:(1) In the research of landscape pattern and ecological process,all landscape types can be divided into two groups,"source"landscape and "sink" landscape."Source" landscape contributes positively to the ecological process,while "sink" landscape is unhelpful to the ecological process.(2) Both landscapes are recognized with regard to the specific ecological process."Source" landscape in a target ecological process may change into a "sink"landscape as in another ecological process.Therefore,the ecological process should be determined before "source"or "sink" landscape were defined.(3) The key point to distinguish "source" landscape from "sink" landscape is to quantify the effect of landscape on ecological process.The positive effect is made by "source" landscape,and the negative effect by "sink" landscape.(4) For the same ecological process,the contribution of "source" landscapes may vary,and it is the same to the "sink"landscapes.It is required to determine the weight of each landscape type on ecological processes.(5) The sourcesink principle can be applied to non-point source pollution control,biologic diversity protection,urban heat island effect mitigation,etc.However,the landscape evaluation models need to be calibrated respectively,because different ecological processes correspond with different source-sink landscapes and evaluation models for the different study areas.This theory is helpful to further study landscape pattern and ecological process,and offers a basis for new landscape index design.     

Application of remote sensing techniques for water quality monitoring

A detailed study and experimental work performed to establish the properties of multispectral remote sensing techniques as applied to the monitoring of water quality, is hihhly desirable since direct synoptic field measurements concerning the quantity and type of pollutants in water is not possible at present.The multispectral characteristics of the signal received from water are a function of hydrological, biological and chemical characteristics of water, and the physical properties of the remote sensor related environment. Spectral data encompassing of the spectral region 0.36 m and 2.36 m showed that the detectibility of water pollutants, using spectral signatures in the 0.4 to 0.9 m range, is promising and feasible.     

Phytoremediative Capacity of Plants Enriched with Melatonin

Melatonin is an environmentally friendly-molecule with broad spectrum antioxidant capacity. Melatonin is widely present in the plant kingdom. High levels of melatonin exist in an aquatic plant, the water hyacinth, which is highly tolerant of environmental pollutants. Elevated levels of melatonin probably help plants to protect against environmental stress caused by water and soil pollutants. To investigate the potential relationships between melatonin supplementation and environmental tolerance in plants, pea plants were treated with high levels of copper in the soil. The results show that copper contamination kills pea plants; however, melatonin added to the soil significantly enhanced their tolerance to the copper contamination and, therefore, increased their survival. Based on the theory and these preliminary data, we speculate that melatonin could be used to improve the phytoremediative efficiency of plants against different pollutants. Since melatonin is safe to animals and humans as well as being inexpensive, it may be a feasible and cost-effective approach to clean environmental contaminations.Key Words: phytoremediation, plants, melatonin, antioxidant     

Soil immobilization: new concept for biotreatment of soil contaminants

A new concept for the development of microbial consortia for the degradation of persistent soil pollutants and for pollutant treatment is proposed. The concept defined as "soil immobilization" is based on the entrapment of soil particles, showing microbial activity in degrading the target pollutant, into a solid membrane with a large pore size distribution. The particular hydrodynamic and mass transfer properties of this system result in a very efficient process. A new type of bioreactor is proposed for carrying out the immobilized soil process. The performance of the system was tested by developing a microbial system for the mineralization of pentachlorophenol (PCP). The results show that the volumetric efficiency of the process for PCP mineralization in the immobilized soil bioreactor is 1-3 orders of magnitude higher than reported literature values. Chlorine and carbon atoms of PCP are both nearly completely (99%) mineralized.     

城市化的生态风险及其管理

城市化体现在人口向城市聚集和城市景观扩张的同时,社会结构发生了极大变化,城市生产生活方式向更加广泛的地区扩散。然而,随着人口的过度密集和城市的日益扩张,交通拥堵、空气污染、废物堆积、流行病肆虐等"城市病"频发。城市通过大量汲取和消耗自然资源,向周围环境排放大量污染物,改变了区域生态系统的原有结构和功能,产生了严峻的生态风险,进而制约着城市生态系统的可持续发展。城市化的生态风险正逐步得到广泛关注,但对其具体的内涵和影响尚不明晰。归类描述了城市化过程中面临的各种生态风险,分析了导致生态风险的主要因素,阐述了辨识与评价生态风险的基本方法,提出了城市化生态风险的调控管理对策。     

Water quality studies on Buckingham Canal (Madras,India) — A discriminant analysis

Stepwise multiple discriminant analysis was applied to a two year water quality monitoring study of the Buckingham Canal at Madras, India. The variables were divided into

1. physical and chemical
2. pollutants; and
3. biological

to test which group is efficient in discriminating stations which differ in water quality. Biological variables obtained higher loadings on the discriminant functions than the other two groups. The derived discriminant functions were tested for their discriminating power through the classification phase with the same raw data. The \lsHit and Misses\rs tables of classification indicate that 81.660% of the samples were correctly classified by the biological variables, against only 46.660% of the samples in the case of physical and chemical variables and 48.33% in the case of pollutants.     

RNA‑seq技术在水生生物生态毒理学中的应用进展

水域是地球环境的重要组成部分,也是最易受污染的生态系统之一。水生态系统中不同营养级别的水生生物可通过摄食、接触等多种途径摄入水体中的污染物。因此,监测水域污染物对水生生物和生态系统的影响,解析污染物对不同水生生物的毒性机制,筛选敏感、有效的生物标志物对生态毒理学研究和环境风险评价具有重要意义。RNA测序（RNA sequencing,RNA?seq）技术因所需样品量少,且不需参考序列,可在整体水平上鉴定基因差异表达,成为水生生物生态毒理学研究的最佳方法之一。基于此,介绍了RNA?seq技术的基本流程与数据分析过程,对该技术在不同生态位的水生生物（如鱼类、两栖类、贝类、甲壳类等）生态毒理学中的应用展开综述,并对RNA?seq技术面临的不足、挑战及发展趋势进行探讨,以期为该技术在水生生物生态毒理学研究中的应用,尤其是水生态环境中污染物胁迫水生生物机制的阐明及污染水域生态环境恢复提供参考。     

Tree pollen viability in areas subject to high pollutant deposition

Summary The wet deposition process, including fog, is a major pathway of acidic pollutants from the atmosphere to the biosphere. Pollen grains are known to be sensitive biological indicators of pollution at this interface. In southern Québec, pollen viability of three hardwood species (sugar maple, white and yellow birch), was significantly reduced above the mean cloud base height (600 m) for two successive years (1987–1988) at two mountain sites (Roundtop and Tremblant). Ozone concentrations, fog water and precipitation pH values over the sampling periods are discussed.     

Bridge processes: a solution for LCI datasets independent of background databases

Introduction

New platforms are emerging that enable more data providers to publish life cycle inventory data.

Background

Providing datasets that are not complete LCA models results in fragments that are difficult for practitioners to integrate and use for LCA modeling. Additionally, when proxies are used to provide a technosphere input to a process that was not originally intended by the process authors, in most LCA software, this requires modifying the original process.

Results

The use of a bridge process, which is a process created to link two existing processes, is proposed as a solution.

Discussion

Benefits to bridge processes include increasing model transparency, facilitating dataset sharing and integration without compromising original dataset integrity and independence, providing a structure with which to make the data quality associated with process linkages explicit, and increasing model flexibility in the case that multiple bridges are provided. A drawback is that they add additional processes to existing LCA models which will increase their size.

Conclusions

Bridge processes can be an enabler in allowing users to integrate new datasets without modifying them to link to background databases or other processes they have available. They may not be the ideal long-term solution but provide a solution that works within the existing LCA data model.

     

Weather patterns and asthma epidemics in New York City and New Orleans,U.S.A.

Twenty-five years of data on asthmatic attacks in New Orleans (covering approximately 170,000 asthma attacks) have been analyzed to identify asthma epidemic days, defined as days on which an unusually high number of asthmatic individuals had attacks. Similar data covering three years was obtained for New York City. A preliminary examination of detailed meteoroligical data revealed a consistent meteorological pattern preceding and associated with such asthma epidemic days which consisted of a cold front preceding an asthma epidemic by one to three days followed by a high pressure system. The significance of these meteorological findings and their relationship to other environmental agents such as natural or man-made atmospheric pollutants that are likely to be associated with asthma attacks will be discussed.Presented at the Eighth International Congress of Biometeorology, 9–14 September 1979, Shefayim, Israel.     

A model useful in deriving standards for environmental pollutants

Abstract

In order to facilitate the informed participation of citizens in the governmental review process, a model is developed to assist students in attempting to establish the biological basis for environmental health standards.

When establishing a standard, the high-risk (or hypersusceptible) segments of the human population should be identified. By setting a standard which protects these portions of the population, the less susceptible portion of the population will also be protected. Factors to be considered when identifying high-risk groups include: hereditary traits, age, nutrition, eating habits, pregnancy, health status, stress, and physical activity.

Whenever possible, studies should be selected as a basis for the standard which have utilized experimental animals which most closely simulate the human population at high risk to the pollutant. Epidemiological studies, when available, should also be utilized as well as controlled studies using human subjects. Extrapolation of data from these types of studies is most reliable. These data can then be utilized to determine a safe level of intake and assimilation of pollutants in the high risk groups. Calculations to determine safe exposure levels vary depending on whether the pollutant is cumulative or not. Once these safe levels are calculated, standards for pollutants can then be recommended for air, food, or water.     

Endocrine disrupting chemicals in mixture and obesity,diabetes and related metabolic disorders

Obesity and associated metabolic disorders represent a major societal challenge in health and quality of life with large psychological consequences in addition to physical disabilities. They are also one of the leading causes of morbidity and mortality. Although, different etiologic factors including excessive food intake and reduced physical activity have been well identified, they cannot explain the kinetics of epidemic evolution of obesity and diabetes with prevalence rates reaching pandemic proportions. Interestingly, convincing data have shown that environmental pollutants, specifically those endowed with endocrine disrupting activities, could contribute to the etiology of these multifactorial metabolic disorders. Within this review, we will recapitulate characteristics of endocrine disruption. We will demonstrate that metabolic disorders could originate from endocrine disruption with a particular focus on convincing data from the literature. Eventually, we will present how handling an original mouse model of chronic exposition to a mixture of pollutants allowed demonstrating that a mixture of pollutants each at doses beyond their active dose could induce substantial deleterious effects on several metabolic end-points. This proof-of-concept study, as well as other studies on mixtures of pollutants, stresses the needs for revisiting the current threshold model used in risk assessment which does not take into account potential effects of mixtures containing pollutants at environmental doses, e.g., the real life exposure. Certainly, more studies are necessary to better determine the nature of the chemicals to which humans are exposed and at which level, and their health impact. As well, research studies on substitute products are essential to identify harmless molecules.     

The organization of the microbial biodegradation network from a systems-biology perspective

Microbial biodegradation of environmental pollutants is a field of growing importance because of its potential use in bioremediation and biocatalysis. We have studied the characteristics of the global biodegradation network that is brought about by all the known chemical reactions that are implicated in this process, regardless of their microbial hosts. This combination produces an efficient and integrated suprametabolism, with properties similar to those that define metabolic networks in single organisms. The characteristics of this network support an evolutionary scenario in which the reactions evolved outwards from the central metabolism. The properties of the global biodegradation network have implications for predicting the fate of current and future environmental pollutants.     

Expression Procedure Optimization of <Emphasis Type="Italic">Carassius aurantus</Emphasis> CYP1A in <Emphasis Type="Italic">Shewanella</Emphasis> Using Plasmid Construction Strategy

Cytochrome P4501A (CYP1A) has attracted an increasing interest due to its important role in metabolism of contaminants in aquatic environment and kinds of biomarkers to monitor the pollutants. CYPs are reported to express in E. coli, yeast and insect cells, while expression levels in these systems are too low to continue further study, such as functional and structural research. In this study, we construct an expression system using Shewanella oneidensis to produce goldfish CYP1A. RBS sequence that can elevate expression level by initiating the translation was added. A leading signal peptide which will direct the goal protein into periplasm of the host was introduced. Moreover, large-size plasmid construction strategy was applied during the successful construction process of expression system. At the position of ~60 kDa, a single band was seen clearly after expression; furthermore the amount of expressed CYP1A was as high as 0.02 micromoles per liter in the culture. Heme test was also performed, the result showed the typical P450 hemoprotein spectra. All these data suggest the possible suitable expression system for fish P4501A system was constructed.     

The phreatic aquifer of the ‘Plana de Castellón’ (Spain):relationships between animal assemblages and groundwater pollution

An applied groundwater study of the Plana de Castellón was performed to investigate possible impacts of pollutants on ecological characteristics of the aquifer. The area is illustrative for a western mediterranean coastal plain, densely populated, industrialized, and with intensive agricultural land-use. During one year, six wells in a subsection of the plain were monitored, simultaneously sampled for zoological and physicochemical analysis. Heavy metals and pesticides were not (or at very low levels) detected in the wells. Low correlations were found between physicochemical and biological data, but there were indications that organic pollution affects the abundance of the fauna. A logarithmic relationship between abundance and COD/BOD₅-ratio is postulated. Salinization, an important threat to the groundwater of the area, was clearly reflected in the chemistry of one of the wells. Probably, this process has important consequences for abundance and diversity of the fauna.