A SUSPECTED CASE OF MYCOPHYCOBIOSIS BETWEEN MYCOSPHAERELLA APOPHLAEAE (ASCOMYCETES) AND APOPHLAEA SPP. (RHODOPHYTA)1

Apophlaea lyallii Hooker f. et Harvey and A. sinclairii Harvey in Hooker et Harvey, endemic Rhodophyta from New Zealand, were found to harbor a systemic fungus, Mycosphaerella apophlaeae Kohlmeyer. The algae occur on rocks in the upper intertidal zone and are resistant to desiccation and insolation. These suspected symbiotic associations which are similar to, but different from, lichens, are considered mycophycobioses. This is the first report of a supposed mycophycobiosis in members of the Rhodophyta.     

THE LIFE HISTORY OF CALLITHAMNION BYSSOIDES IN CULTURE

The life history of Callithamnion byssoides Arnott ex Harv. in Hook. has been shown to comprise a regular sequence of gametophytic, carposporophytic, and tetrasporophytic phases in unialgal culture using supplemented seawater media. Tetraspore germlings gave rise to gametophytes bearing either antheridia or carpogonia in 13 days. Fertilization, carposporophyte development, and carpospore release took place within 5 days. Carpospore germlings produced mature tetrasporophytes in 13 days. The life history thus required approximately 1 month for completion. No deviations from this pattern were observed.     

FIELD AND CULTURE STUDIES ON GROWTH AND REPRODUCTION OF CALLITHAMNION BYSSOIDES (RHODOPHYTA,CERAMIALES) IN NORTH CAROLINA1,2

The life history of Callithamnion byssoides Arnott ex Harv. in Hook. from North Carolina is shown to be of the Polysiphonia-type. Cross-gradient light-temperature culture in 8:16 and 14:10 LD cycles was utilized to investigate the effects of light intensity, temperature and day-length on growth and reproduction. Carposporophyte and tetrasporangium development were controlled by total light energy rather than photoperiod. Results of these studies explain the observed seasonal periodicity of growth and reproduction on the North Carolina coast. Variations between these results and previously published data for this species in Texas are explained in terms of ecotypic differentiation.     

SYNARTHROPHYTON,A NEW GENUS OF CORALLINACEAE (CRYPTONEMIALES,RHODOPHYTA) FROM THE SOUTHERN HEMISPHERE1

Synarthrophyton gen. nov. is described based on southern Australian material of Melobesia patena Hooker fils & Harvey, which shows features intermediate between Mesophyllum Lemoine and Lithothamnium Philippi. It has the thin-walled rounded epithallial cells and coaxial hypothallium characteristic of Mesophyllum and the type of procarp and male structures common to Lithothamnium. Synarthrophyton is unique within the subfamily Melobesioideae (J. E. Areschoug) Yendo because it displays secondary pit connections. Details of the vegetative and reproductive morphology of S. patena (Hooker fils. & Harvey) comb. nov. are given and its taxonomic position in the Corallinaceae Lamouroux discussed.     

The Music Indici: its authors, types and localities

The sixty new taxa described in Musci Indici are reviewed. As a result of validation of 54 of these by plates alone, authorities, original specimens and provenance of many have been widely misinterpreted. Evidence from published material, unpublished correspondence and herbarium material demonstrates that W. H. Harvey (1836) is the sole validating author of most of the names; three were validated by J. D. Hooker in 1837, and two names jointly by J. D. Hooker and Harvey in 1840. None was validated by W. J. Hooker, in the past often considered to be the author of some of the names. It is shown that Musci Indici names should be typified by lectotypes; recent typifications of four of the names by ‘holotypes’ and six out of eight published ‘lectotypes’ are untenable as the specimens selected were not original material studied by Harvey, and should be superseded by new lectotypes. Five specimens are selected as new lectotypes. Original material, mostly in Harvey's herbarium in TCD, is identified as most suitable for future lectotypification of the other names. Types for the three names based solely on Royle material are located in LIV and BM. Original Harvey material is also preserved in GL, but most of the Wallich collections in BM, E and elsewhere are not part of the original material. For Neckera blanda no suitable type specimen was located; the original published plate is selected as lectotype with a specimen in BM as a supporting ‘epitype’. Many of the original localities published in 1840 as ‘Nepal’ are shown to be erroneous. As far as possible these are corrected but for some taxa provenance remains doubtful. Twenty-two of the new names are shown not to have been based on material from Nepal; as a result ten species (Acanthorrhynchium papillatum, Brachythecium kamounense, Chaetomitriopsis glaucocarpa, Meiothecium microcarpum, Mitthyridium repens, Rozea fulva, Splachnobryum flaccidum, Sterophyllum radiculosum, Trichosteleum boschii and Trismegistia lancifolia) and Trismegistia lancifolia) are deleted from the Nepal checklist. Twenty-eight of the new species are considered to be reliably based on material from Nepal, and a further five doubtfully so. Rozea microcarpa Broth. is shown to be an synonym of R. fulva (Harv.) M. Fleisch. Past taxonomic confusion between Hypnum cordatum Harv. and Neckera cordata [Hook. ex] Harv. is untangled; the first is shown to be a synonym of Eurhynchium hians (Hedw.) Sande Lac. and the second is the basionym of Penzigiella cordata (Harv.) M. Fleisch.     

TAXONOMY OF ADAMSIELLA L.E. PHILLIPS ET W.A. NELSON,GEN. NOV. AND EPIGLOSSUM KÜTZING (RHODOMELACEAE,CERAMIALES)1

In a taxonomic/phylogenetic study of the genus Lenormandia, several species were found to differ significantly from the type species, L. spectabilis Sonder, in apical morphology, blade‐surface pattern, medullary construction, the presence of pseudopericentral cells, and the position of reproductive structures. These species constitute two groups that differ morphologically, a finding largely supported by analysis of 18S rRNA sequences, as reported previously. The two putative Lenormandia species from New Zealand, along with two previously undescribed species also from New Zealand, comprised one such group, designated here by the new genus name Adamsiella L.E. Phillips et W.A. Nelson, gen. nov. and including A. melchiori L.E. Phillips et W.A. Nelson, sp. nov., A. lorata L.E. Phillips et W.A. Nelson, sp. nov., A. angustifolia (Harvey) L.E. Phillips et W.A. Nelson, comb. nov., and A. chauvinii (Harvey) L.E. Phillips et W.A. Nelson comb. nov. Adamsiella differs from Lenormandia by incurved apices, a chevron surface pattern, and reproductive structures on dorsi‐ventrally flattened apically incurved polysiphonous branchlets usually produced at the margins. Two species endemic to Australia formed the second group designated by the resurrected generic name Epiglossum and also characterized by a strongly incurved apex and chevron surface pattern but with reproductive structures produced on terete polysiphonous branchlets found either on the midrib or elsewhere on the blade surface but not the margins. Epiglossum contains E. smithiae (J.D. Hooker et Harvey) Kützing and E. proliferum (C. Agardh) L.E. Phillips, comb. nov.     

137 Gamete Recognition and Sexual Isolation in a Red Alga,Aglaothamnion Byssoides (Rhdophyta)

The binding of fluorescein isothiocyanate (FITC) conjugated lectins to gametes of Aglaothamnion byssoides Itono during the fertilization was studied by the use of confocal microscope. The physiological effects of lectins and carbohydrates on gamete binding were also examined. Three lectins, concanavalin A (ConA), Soybean agglutinin (SBA) and wheat germ agglutinin (WGA) bound to the surface of spermatia, but each lectin labeled different region of the spermatium. SBA bound only to the spermatial appendages but ConA bound to the whole spermatial surface except spermatial appendages. WGA labeled narrow region which connects spermatial body and appendages. During fertilization, ConA and WGA specific substances on the spermatial surface moved towards the area contacting with trichogyne and accumulated on the surface of fertilization canal. Spermatial binding to trichogynes was inhibited by pre‐incubation of spermatia with SBA, while trichogyne receptors were blocked by the complementary carbohydrate, N‐acetyl‐D‐galactosamine. WGA and its complementary carbohydrate had little effect on gamete binding. For searching the step of sexual isolation, crossing experiment was performed between Aglaothamnion byssoides and twelve other red algal species. Results showed that the gamete recognition was genus‐specific: the gametes bound freely with their partners of the same genus. When two species from same genus were crossed, sexual isolation occurs gradually during the fertilization process. Therefore, sexual isolation in red algae appears to be determined by multi‐step process and gamete binding is the initial step.     

角唇隔距兰,中国隔距兰属(兰科)一新记录种

报道了中国兰科(Orchidaceae)隔距兰属(Cleisostoma Blume)一新记录种:角唇隔距兰(C.tricornutum Averyanov)。该种与C.crochetii(Guillaumin)Garay、C.parishii(J.D.Hooker)Garay相近,因其有不分枝的线状花粉团柄、球状的花粉团块和一个先端短喙状的半球形药帽而与C.crochetii(Guillaumin)Garay相区别;因其有松散、罕分枝的花序,长角状的唇瓣侧裂片而与C.parishii(J.D.Hooker)Garay相区别。提供了新记录种的形态描述及图片。     

Mastophoropsis canaliculata (Harvey in Hooker) gen. et comb. nov. (Corallinaceae,Rhodophyta) in Southern Australia

Mastophoropsis canaliculata (Harvey in Hooker) gen. et comb. nov. (Corallinaceae, Rhodophyta) is restricted to south-eastern Australian waters. It is unique among Corallinaceae in possessing an erect, tenacular, branched, taeniform, non-geniculate thallus which produces multiporate tetrasporangial conceptacles. Based on a detailed morphological and anatomical study, including an examination of the designated lectotype, this taxon is referred to the tribe Phymatolitheae in the subfamily Melobesioideae and its relationships to other non-geniculate Corallinaceae are discussed. A simplified microtechnique procedure involving decalcification with nitric acid, resin embedding and staining serially mounted sections with KMnO₄ also is outlined. X-ray microanalysis of surface tissues indicates that calcification occurs largely as CaCO₃ and that various structures contain substantially differing amounts of Ca.     

CROSSING EXPERIMENTS,LIPID COMPOSITION,AND THE SPECIES CONCEPT IN ECTOCARPUS SILICULOSUS AND E. FASCICULATUS (PHAEOPHYCEAE,ECTOCARPALES)1

Sporophytes of Ectocarpus siliculosus (Dillwyn) Lyngbye and E. fasciculatus Harvey were collected in the vicinity of Roscoff Brittany, France. Gametophytes derived from meiospores were used for intra- and interspecific crosses. Intraspecific gamete combinations gave viable zygotes, which developed into fertile sporophytes. Interspecific crosses were unsuccessful. Gamete fusions did not occur between female gametes of E. fasciculatus and male gametes of E. siliculosus. Hybrid zygotes were formed in the reciprocal combination but died soon after germination. We conclude that the two species of Ectocarpus at Roscoff represent distinct taxonomic entities, which are separated by pre- and postzygotic compatibility barriers. These biological findings are confirmed by the differential occurrence of the chemotaxonomic marker betaine-lipid diacetylglycerylhydroxymethyltrimethyl-β-alanine, which is present in our cultures of E. fasciculatus but absent in E. siliculosus.     

Mobile epiphytic invertebrates inhabiting the brown macroalga, Desmarestia chordalis, under the coastal fast ice of Lützow-Holm Bay, East Antarctica

The species composition of mobile epiphytic animals inhabiting the brown alga, Desmarestia chordalis Hooker and Harvey, in Lützow-Holm Bay, East Antarctica, was investigated. A total of 797 individual invertebrates in 22 taxa were collected from 3 clumps of algae. The assemblage was dominated by two amphipod crustaceans, Prostebbingia sp. and Haplocheira plumosa Stebbing, which composed 70.3% of the total number of individuals, followed by a gastropod species, Skenella paludinoides (Smith). The species composition differs from those found in previous studies conducted in Prydz Bay and Ross Sea, suggesting the existence of various types of epifaunal communities along the East-Antarctic coast.     

中国鸢尾兰属(兰科)植物二新记录种

该文报道了兰科(Orchidaceae)鸢尾兰属(Oberonia Lindl.)2个中国新记录种,即心唇鸢尾兰(O. orbicularis Hooker f.)和凭祥鸢尾兰(O. delacourii Gagnep.)。其中,心唇鸢尾兰(O. orbicularis)与国内分布的全唇鸢尾兰(O. integerrima)和扁葶鸢尾兰(O. pachyrachis)均较为相似,但该种花葶中下部两侧具狭翅,不与叶合生,唇瓣边缘具明显的啮蚀状齿或细流苏状,先端中部具明显凹缺,可明显区别。心唇鸢尾兰(O. orbicularis)于2013年首次发现于云南省勐腊县,为中国新记录种。凭祥鸢尾兰(O. delacourii)与剑叶鸢尾兰(O. ensiformis)最为相近,但前者唇瓣中裂片先端小裂片近方形,小裂片先端边缘具流苏状齿,可明显区别于后者。凭祥鸢尾兰自1929年发表以来,除模式产地老挝外,至今未见新分布点的报道,为中国新记录种。文中提供了两个新记录种的形态特征描述,凭证标本存放于中国科学院华南植物园标本馆(IBSC)。     

Genetic and morphological variation in an ecosystem engineer,Lithophyllum byssoides (Corallinales,Rhodophyta)

Lithophyllum byssoides is a common coralline alga in the intertidal zone of Mediterranean coasts, where it produces biogenic concretions housing a high algal and invertebrate biodiversity. This species is an ecosystem engineer and is considered a target for conservation efforts, but designing effective conservation strategies currently is impossible due to lack of information about its population structure. The morphological and molecular variation of L. byssoides was investigated using morphoanatomy and DNA sequences (psbA and cox2,3) obtained from populations at 15 localities on the Italian and Croatian coasts. Lithophyllum byssoides exhibited a high number of haplotypes (31 psbA haplotypes and 24 cox2,3 haplotypes) in the central Mediterranean. The psbA and cox2,3 phylogenies were congruent and showed seven lineages. For most of these clades, the distribution was limited to one or a few localities, but one of them (clade 7) was widespread across the central Mediterranean, spanning the main biogeographic boundaries recognized in this area. The central Mediterranean populations formed a lineage separate from Atlantic samples; psbA pair‐wise divergences suggested that recognition of Atlantic and Mediterranean L. byssoides as different species may be appropriate. The central Mediterranean haplotype patterns of L. byssoides were interpreted as resulting from past climatic events in the hydrogeological history of the Mediterranean Sea. The high haplotype diversity and the restricted spatial distribution of the seven lineages suggest that individual populations should be managed as independent units.     

VARIATION IN OCHLOCHAETE HYSTRIX (CHAETOPHORALES. CHLOROPHYYCEAE) STUDIED IN CULTURE1

Six different isolates of Ochlochaete Thwaites ex Harvey have been studied under identical culture conditions. All the isolates show open branching, a character previously ascribed specifically to O. hystrix Thwaites ex Harv. sensu stricto, and all form hairs on rounded cells in the central part of the thallus, a character hitherto attributed only to O. ferox Huber. Consequently, separation of these two entities on the species level is untenable. The plant described by Huber is referred to O. hystrix var. ferox (Huber) var. nov. The type material of Chaetobolus gibbus Rosenvinger is similar to one of the isolates studied and is included in O. hystrix. Quadriflagellate zoospores have been observed in all the isolates, and in one of them also biflagellate swarmers.     

New records of temperate and sub-Antarctic marine benthic macroalgae from Antarctica

 During visits to several localities on the South Shetland Islands and the Antarctic Peninsula in summer 1994 we made collections of marine benthic algae. Among the algae we collected were three new records for Antarctica [Petalonia fascia (OF Müller) Kuntze; Enteromorpha intestinalis (L.) Nees; Rhodymenia subantarctica Ricker] and seven other species [Scytosiphon simplicissimus (Clemente) Cremades; Chordaria linearis (Hooker et Harvey) Cotton; Halopteris obovata (Hooker et Harvey) Sauvageau; Acrosiphonia arcta (Dillwyn) J. Agardh; Enteromorpha compressa (L.) Nees; Bangia atropurpurea (Roth) C. Agardh; Porphyra plocamiestris Ricker] that had been reported on only one or two previous occasions (or, in one case, three). The ten species detailed in this paper fall into two groups: four species previously known from sub-Antarctic islands and/or locations in southern South America, and six species having a wider distribution in temperate regions. We discuss the possibility that the less accessible subtidal habitats of some species may have prevented earlier discovery. Other species may be comparatively recent adventives, most likely introduced with shipping. In view of possible global climate changes, species of this latter group are regarded as suitable organisms for monitoring changes of water temperature. Received: 12 December 1995/Accepted: 14 April 1996     

Observations on Japanese species of the genus Martensia (Delesseriaceae, Rhodophyta), with the description of Neomartensia gen. nov.

The morphology of vegetative and reproductive structures of Martensia fragilis Harvey, Martensia australis Harvey and Martensia flabelliformis Harvey ex J. Agardh was studied based on material collected from Japan. M. fragilis and M. australis have cystocarps with terminal carposporangia, and their membranous portions are composed of cell layers with regularly stacked flat cells of similar size. M. flabelliformis has the following combination of characters: (i) carposporangia are formed in short chains; and (ii) the membranous portion is composed of irregularly arranged flat cells of various sizes. These characters are distinctly different from those of Martensia and warrant the establishment of Neomartensia gen. nov., with the type species Neomartensia flabelliformis comb. nov.     

I. Pathogenic variation in fungi and bacteria and mycorrhizal compatibility: Intraspecific variation in a mycorrhizal association

Laboratory studies with combinations of the fly agaric, Amanita muscaria (L. ex Fries) Hooker and birch (Betula pendula (Roth.)) suggest that the genetical control of mycorrhizal formation has features in common with the legume/Rhizobium symbiosis. There are at least four factors that can be genetically controlled by either the host or the fungus: (a) mycorrhizal formation; (b) the extent of mycorrhizal development; (c) the pattern of mycorrhizal branching, and (d) the shape and size of the host root system.     

Contribution to the seaweed flora of Namibia: new records of Chlorophyta and Phaeophyceae

Six species of benthic marine Chlorophyta and Phaeophyceae are recorded for the first time from the coast of Namibia: Ectocarpus fasciculatus Harvey, Feldmannia irregularis (Kützing) G. Hamel, Bachelotia antillarum (Grunow) Gerloff, Straguloria clavata (Harvey in Hooker) Hamel, Entocladia leptochaete (Huber) Burrows and Ulvella lens P. Crouan & H. Crouan. Data concerning local and world distribution of these species as well as some taxonomical remarks are provided.     

On the sexual reproduction ofDictyosiphon foeniculaceus (Phaeophyceae,Dictyosiphonales)

Dictyosiphon foeniculaceus from Sweden and Newfoundland was studied in laboratory culture. Zoids from unilocular sporangia developed into dioecious microscopic filamentous gametophytes which produced uniseriate plurilocular gametangia in low temperatures (0 to 8 °C). Zygotes and unfused isogametes gave rise to filamentous protonemata on which parenchymatous macroscopic sporophytes were formed. Isolates from Sweden and Newfoundland were interfertile. Although formed in culture, genetically unisexual sporophytes were not detected in nature. Female gametes ofD. foeniculaceus produced a sexual pheromone. It was identified as finavarrene, which is also known as the sperm attractant inAscophyllum nodosum.