共查询到20条相似文献,搜索用时 31 毫秒
1.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1992,101(3):641-643
- 1.1. African sharptooth catfish individuals, heterozygous for glucose phosphate isomerase (GPI-2), were selected as broodstock by using horizontal starch-gel electrophoresis.
- 2.2. Ova of one heterozygous female were inseminated with cryopreserved and fresh milt from a corresponding male.
- 3.3. Significant deviations from expected Hardy-Weinberg proportions occured for offspring obtained by using cryopreserved milt.
- 4.4. Differences in genotypic variation seems to relate to different cryodiluents and the fertility thereof.
- 5.5. Selection of breeding stock for aquacultural practices based on the above information is discussed.
2.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1989,92(3):487-492
- 1.1. Subcellular distribution of (NA+, K+-ATPase and ouabain-insensitive ATPase (Mg2+-ATPase) are compared in branchial tissues of the euryhaline crab, Eriocheir sinensis, acclimated to fresh water.
- 2.2. Both the anterior and posterior gills contain cAMP-dependent protein kinase and endogenous protein substrate for phosphorylation.
- 3.3. Phosphorylation occurs in both “particulate” and “soluble” subcellular fractions but its stimulation by cAMP is restricted to the “soluble” fraction.
- 4.4. serotonin (5-HT) and dopamine receptors are present only in the “light particulate” fraction isolated from the posterior gills.
- 1.(a) Serotonin and dopamine have no effect on the phosphorylation observed in a subcellular fraction alone.
- 2.(b) Activation of the phosphorylation by serotonin and dopamine is found when the soluble fraction (source of cAMP-dependent protein kinase) is added to the fraction P3 from the posterior gills.
- 3.(c) No activation occurs with the fractions P3 as well as P1 or P2 (not shown) from anterior gills of fresh water crab.
- 4.(d) Cyproheptadine, a serotonin receptor antagonist, inhibits the 5-HT dependent increase in phosphorylation.
- 5.(e) The dopamine receptor antagonist, chlorpromazine, inhibits dopamine-stimulated phosphorylation.
- 6.5. Ouabain mimics the effect of cyproheptadine on the serotonin-stimulated phosphorylation found in the posterior gills.
3.
《Comparative biochemistry and physiology. A, Comparative physiology》1989,92(2):247-253
- 1.1. In sea-water, adult salmon (S. salar) exchange an average of 12.6% of total body sodium/hr.
- 2.2. Following transfer to fresh water sodium uptake follows Michaelis-Menton kinetics. Fmax = 2.40 mmol Na/1 ECF/hr, Km = 0.26 mmol Na/1. The uptake system is fully activated immediately following transfer to fresh water.
- 3.3. Post smolts adapted to sea-water for 3 months take up sodium at only one third of the rate of adult fish following return to fresh water.
- 4.4. The concentration of prolactin in the plasma is low in sea-water adapted fish and does not rise during the first 8 hr in fresh water.
- 5.5. At pH 5 sodium uptake is reduced by almost 90%, even in the absence of aluminium, but recovers immediately on return to neutral water.
- 6.6. At pH 5 and 20 μmol Al/1 there is little further effect on sodium uptake but after 6 hr in aluminium the inhibition of sodium uptake continues after return to neutral aluminium fresh water and uptake is only 50% of normal 24 hr later.
4.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1987,86(2):497-501
- 1.1. Main serum α1-protein (α1P) of rainbow trout was purified and its biochemical and physico-pathological properties were studied.
- 2.2. α1P was suggested to be a primitive protein having both properties of albumin and AFP in serum proteins of mammals according to the following results.
- 3.3. Molecular weight (75,000), two kinds of molecules (pI 4.55 and 5.05) and amino acid composition.
- 4.4. Dye- or ConA binding activity.
- 5.5. Estrogen binding activity and inhibitory effect on lymphoblastoid-forming activity.
- 6.6. Possible osmotic regulator.
- 7.7. Significant elevation of blood α1P level in the course of hepatoma induction.
5.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1986,83(4):783-785
- 1.1. Seven natural populations of Dacus dorsalis were analyzed for a dimeric esterase by means of horizontal starch-gel electrophoresis.
- 2.2. The electrophoretic phenotypes were governed by nine codominant Est-D alleles.
- 3.3. The commonest allele in all seven population samples was Est-D100 which encoded an electrophoretic band with intermediate mobility.
- 4.4. The distribution of EST-D phenotypes were in accordance with Hardy-Weinberg expectations.
- 5.5. There was no geographic variation in the distribution of Est-D alleles.
6.
《The International journal of biochemistry》1993,25(3):325-329
- 1.1. Hyaluronic acid (HA) can be digested with a Streptomyces hyaluronidase.
- 2.2. The rate of production and the ratio of tetrasaccharide (T) and hexasaccharide (H), studied by HPLC, varied with the temperature and duration of hydrolysis.
- 3.3. The rates of production and the respective amounts of the two oligosaccharides depended on the rheological properties of the HA from different sources.
- 4.4. A close relationship was found between the initial rate of hydrolysis and the intrinsic viscosity of the HA (ηi).
- 5.5. Our data suggest that enzymatic degradation at a given pH value, temperature, and duration of hydrolysis is dependent on the conformation of HA.
- 6.6. Moreover, under given conditions, the relative proportions of the two oligosaccharides depend on the ηi and may also reflect the degree of hydrolysis of the substrate.
7.
《The International journal of biochemistry》1989,21(12):1337-1342
- 1.1. A novel glycogen phosphorylase inhibitor was partially purified from crayfish hepatopancreas.
- 2.2. The inhibitor was found only in two species of crayfish examined, and not in lobster, fresh and salt water clams, mussels or cockroaches.
- 3.3. The inhibitor is a small protein (Mr = 23,000) which did not show proteolytic activity.
- 4.4. Preliminary kinetic analysis of the inhibitory mechanism indicated that it bound to both glycogen and the glycogen phosphorylase protein.
- 5.5. Inhibitor binding to glycogen resulted in a competitive inhibition pattern with respect to glycogen phosphorylase (inhibition constant of ca 10 μg/ml).
- 6.6. The inhibitor also bound glycogen phosphorylase directly with a binding coefficient of 100 μg/ml resulting in a partially non-competitive inhibition pattern with respect to phosphate.
8.
《Comparative biochemistry and physiology. A, Comparative physiology》1986,83(4):761-764
- 1.1. Two columnar cacti in the Sonoran Desert, agria and organpipe, contain medium chain (C8−C12) fatty acids.
- 2.2. Necrotic tissues of these cacti serve as feeding and breeding substrates for Drosophila mojavensis but not D. nigrospiracula.
- 3.3. Results show that capric and lauric acids are the predominant fatty acids of both cacti.
- 4.4. Fatty acid chain length exhibits a differential effect on larval viability with caprylic acid (Q) having the greatest and myristic acid (C14) having the least effect.
- 5.5. Drosophila mojavensis is more tolerant of free fatty acids than D. nigrospiracula, and this partly explains the ability of D. mojavensis to utilize agria and organpipe cacti.
9.
《Comparative biochemistry and physiology. A, Comparative physiology》1992,101(3):451-454
- 1.1. Kidney, oesophagus and gill Na+-K+ ATPase activity and serum Na+, K+ and Cl− concentrations are evaluated in European sea bass during experimental acclimation to fresh water.
- 2.2. Kidney and oesophagus ATPase increase in low salinity and reach a maximum in fresh water.
- 3.3. Gill ATPase decreases during the acclimation trials and rises again to normal values after a 3-week stay in fresh water.
- 4.4. Na+ and K+ serum concentrations decrease during the trials and increase back after a 3-week stay in fresh water.
- 5.5. The correlations between enzymatic activities, serum ion concentrations, morphological changes and environmental salinity are discussed.
10.
《The International journal of biochemistry》1993,25(5):627-630
- 1.1. Phosphatase activities were determined in various seminal fluid and plant tissues.
- 2.2. Human semen showed a markedly higher phosphatase activity, and some plants and mushrooms exhibited a considerably higher phosphatase activity.
- 3.3. Phosphatase purified from human seminal fluid showed a pH optimum of 5–6 and was potently inhibited by l-(+)-tartrate.
- 4.4. Tartrate could not inhibit most plant phosphatases, but inhibited the mushroom phosphatase with a one-order lower affinity than that of the seminal enzyme.
11.
《Biometric Technology Today》2003,11(11):6
- Daon
- Musicrypt
- EMI Music Canada
- Digital Broadband Networks
- FaceKey Corporation
- Eystar Media Inc (EMI)
- Temasya Wira
- Animated Electronic Industries
- BIO-key International
- Entryport Corporation
12.
《Molecular & cellular proteomics : MCP》2019,18(7):1437-1453
- Download : Download high-res image (139KB)
- Download : Download full-size image
- •mRNA-seq, miRNA-seq, proteomes of P. fulvidraco, P. vachelli, hybrid Huangyou-1.
- •Predicted miRNA-mRNA-protein pairs were found and validated by qRT-PCR and PRM.
- •Immune, metabolism, digestion, absorption, proliferation, development generate heterosis.
- •High parental gene/protein with low parental miRNAs inherit from the mother or father.
13.
《Biometric Technology Today》2003,11(10):5
Including information on:
- ScanSoft
- SpeechWorks International
- Viisage Technology
- Firstec
- BIO-key International
- HP
- ZN Vision Technologies
- Unisys
- US Government’s
- Communication Intelligence Corporation
- Infinity Technologies
14.
《Molecular & cellular proteomics : MCP》2019,18(8):1630-1650
- Download : Download high-res image (106KB)
- Download : Download full-size image
- •Our search identifies 2,134 kinase-substrate phosphosite pairs in breast cancer.
- •CDKs and MAPKs are dominant regulators of trans substrate-phosphorylation.
- •Druggability, outcomes, and immune signatures related to kinase-substrates.
- •Experimentally validated activated phosphosites of ERBB2, EIF4EBP1, and EGFR.
15.
《Comparative biochemistry and physiology. A, Comparative physiology》1986,83(3):489-493
- 1.1. Fundamental chitin digestion characteristics of Crassostrea virginica crystalline style were investigated.
- 2.2. Optimum temperature and pH were 34°C and 4.8. respectively.
- 3.3. The colloidal regenerated chitin (0.56mol/0.5 ml: GlcNAc equivalents) was saturating under all enzyme levels encountered.
- 4.4. There was no evidence of end product inhibition, even after 100 hr incubation.
- 5.5. Calculated Km for the chitinase complex was 1.19mM when determined using a 30 min assay, but was only 0.70 mM when determined using a 4.6 hr assay.
- 6.6. Both Km values are lower than reported for similar assays in other molluscs and for most bacteria.
- 7.7. Effect of substrate preparation on the kinetics are discussed.
- 8.8. Eight peaks of chitinase activity were resolved by DEAE-Fractogel ion exchange chromatography.
16.
《The International journal of biochemistry》1994,26(2):155-162
- 1.1. Pseudomonas aeruginosa phospholipase C from culture supernatants of bacteria grown in high-Pi basal salt medium with choline, as the sole carbon and nitrogen source, was purified by precipitation with 70% saturation ammonium sulfate in the presence of celite.
- 2.2. The PLC activity was eluted of this mixture by the use of a reverse gradient of 70-0% ammonium sulfate.
- 3.3. The peak containing the PLC activity revealed a single protein after SDS-PAGE.
- 4.4. The method could also be applied to purify PLC produced in a low-Pi complex medium. The resultant preparation was not homogeneous.
- 5.5. The molecular weight for both PLC preparations was about 70 kDa.
- 6.6. Both PLC used phosphatydilcholine and sphingomyelin as substrates, displayed hemolytic activity an exhibited an apparent KM of 25 mM for p-nitrophenylphosphorylcholine.
- 7.7. They were not inhibited by 1% sodium deoxycholate but were 30% inhibited by 1% Triton X-100.
- 8.8. 2% sodium dodecylsulfate and 1% tetradecyltrimethylammonium bromide inhibited the PLC from the HPl-BSM plus choline but not the enzyme from the LPl-CM.
17.
《Biometric Technology Today》2003,11(10):3
Including information on:
- Martin State Airport
- Bioscrypt
- Saflink
- Office of the Secretary of Defense
- Department of Defense
- Boeing Corporation
- Bell ID, Gemplus
- Siemens
- Foreign Ministry
18.
《Biometric Technology Today》2003,11(9):5
- Bioscrypt
- Saflink
- Dell
- Fujitsu Microelectronics America
- Identix
- Viisage
- Acsys Biometrics
- US Government
19.
《The International journal of biochemistry》1985,17(3):341-345
- 1.1. A new tetralysine endopeptidase from Escherichia coli AJ005 has been purified about 135-fold.
- 2.2. The peptidase seems to be specific to tetralysine among lysine homopolymers.
- 3.3. The optimal pH was about 7.5
- 4.4. The activity was inhibited by KCN but not inhibited by soybean trypsin inhibitor.
- 5.5. The apparent Km value was 2.5 × 1O−3 M for tetralysine.
20.
- 1.1. The oxygen consumption of crabs in normoxic and hypoxic (50% O2) seawater was measured directly after collection.
- 2.2. The influences of size and lunar cycles were removed by scaling the data.
- 3.3. Strong negative correlations between low individual levels of O2 consumption and the ability to compensate for hypoxia were apparent in Wicklow (subtidal) crabs.
- 4.4. Compensation for hypoxia was much greater on the flood tide than on the ebb.
- 5.5. Crabs from Roscoff (intertidal) had lower levels of compensation than those from Wicklow.
- 6.6. Size, sex and condition had no apparent effect upon these relationships.
- 7.7. Crabs acclimated to laboratory conditions have not shown this tidal variation in compensation for hypoxia.