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1.
《Journal of Fermentation and Bioengineering》1995,79(2):174-176
In a large amount of porphyrins produced by a bacterium isolated from soil, Arthrobacter hyalinus, cultured in a medium containing isopropanol as the sole carbon source, zinc porphyrins, identified based on the coincidence between their m/z values in LC/MS and the molecular weight of porphyrins, were also found to be produced. Since zinc is easily separated from porphyrins in acid during the esterification of porphyrins, zinc uroporphyrin III was prepared from its octamethyl ester formed by incorporating zinc into the octamethyl ester of uroporphyrin III which was isolated from the culture broth. Its UV spectra, fluorometric spectra, fast atom bombardment (FAB)-mass spectra, and 1H-NMR and 13C-NMR spectra were presented. 相似文献
2.
Sunita Singh Moreau Eric Inman Floyd Holmes D. Leonard 《Indian journal of microbiology》2012,52(3):325-331
Culturing the bioluminescent bacterium Photorhabdus luminescens in nutrient broth (NB) is used to recover phase I cells. These phase I cells were highly luminescent for up to 7 h in this media and the luminosity could also be seen with the naked eye after a 15 min eye adjustment period in a dark room. Red pigmentation is a known trait of phase I cells and was visually distinct within the culture media. The color shade of the red pigment varied on nutrient agar and in NB suggesting that the concentration of the pigment produced is dependent upon density of phase I cells within a specified area. The specific growth rate (μ) and doubling time (g) was determined during the logarithmic growth phase to be 0.36 h−1 and 2.1 h, respectively in NB medium. The nematode-bacterium suspension was injected into larvae of Galleria mellonella to test for entomopathogencity. Within 24 h post-injection insect mortality was seen along with dark red pigmentation and extremely high luminosity indicating infection with P. luminescens. 相似文献
3.
The porphyrin and phorbin biosynthetic activity of etiolated cucumber (Cucumis sativus, L.) cotyledons was compared to that of cotyledonary homogenates. Etiolated cotyledons incubated with δ-aminolevulinic acid accumulate protoporphyrin, coproporphyrin, small amounts of Mg protoporphyrin monoester, and trace amounts of uroporphyrin. They also incorporate 4-14C-δ-aminolevulinic acid into free porphyrins, protochlorophyllide, protochlorophyllide phytyl ester, and Mg protoporphyrin monoester. Homogenates incubated with δ-aminolevulinic acid likewise accumulate coproporphyrin, uroporphyrin, Mg coproporphyrin, and trace amounts of protoporphyrin. They also incorporate 4-14C-δ-aminolevulinic acid into Mg protoporphyrin monoester, Mg coproporphyrin, and free porphyrins. However, the capacity to synthesize protochlorophyllide and protochlorophyllide phytyl ester is lost and the endogenous protochlorophylls gradually disappear. Mg protoporphyrin monoester represents the terminal biosynthetic step in this cell-free system. 相似文献
4.
1. The partition of uroporphyrins I and III, coproporphyrins I and III, haematoporphyrin IX, porphyrin c and a hydrophilic porphyrin–peptide fraction from variegate-porphyria faeces has been studied in systems of equal volumes of cyclohexanone and sodium acetate buffers of varying pH and concentration. 2. The concentration of acetate in the aqueous phase has little effect on the partition of porphyrin c, but markedly influences that of uroporphyrin. At 50% acetate saturation and pH4·5, only 5% enters the cyclohexanone phase whereas 60% of porphyrin c is extracted under similar conditions. 3. This circumstance forms the basis of a method for the determination of hydrophilic porphyrin–peptides in variegate-porphyria urine. Its reliability has been checked in model experiments. 4. At pH1·5 and an aqueous phase half-saturated with sodium acetate, an equal volume of cyclohexanone removes 95–97% of uroporphyrin and about 55% of porphyrin c. Uroporphyrin may therefore be determined as a second step in the method. 5. For the routine determination of uroporphyrin in systems free from other hydrophilic porphyrins, cyclohexanone extraction may be performed at any pH in the range 1·0–3·0. 相似文献
5.
Medium-Chain Fatty Acids Affect Citrinin Production in the Filamentous Fungus Monascus ruber 总被引:4,自引:0,他引:4
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Hassan Hajjaj Alain Klab Grard Goma Philippe J. Blanc Estelle Barbier Jean Franois 《Applied microbiology》2000,66(3):1120-1125
During submerged culture in the presence of glucose and glutamate, the filamentous fungus Monascus ruber produces water-soluble red pigments together with citrinin, a mycotoxin with nephrotoxic and hepatoxic effects on animals. Analysis of the 13C-pigment molecules from mycelia cultivated with [1-13C]-, [2-13C]-, or [1,2-13C]acetate by 13C nuclear magnetic resonance indicated that the biosynthesis of the red pigments used both the polyketide pathway, to generate the chromophore structure, and the fatty acid synthesis pathway, to produce a medium-chain fatty acid (octanoic acid) which was then bound to the chromophore by a trans-esterification reaction. Hence, to enhance pigment production, we tried to short-circuit the de novo synthesis of medium-chain fatty acids by adding them to the culture broth. Of fatty acids with carbon chains ranging from 6 to 18 carbon atoms, only octanoic acid showed a 30 to 50% stimulation of red pigment production, by a mechanism which, in contrast to expectation, did not involve its direct trans-esterification on the chromophore backbone. However, the medium- and long-chain fatty acids tested were readily assimilated by the fungus, and in the case of fatty acids ranging from 8 to 12 carbon atoms, 30 to 40% of their initial amount transiently accumulated in the growth medium in the form of the corresponding methylketone 1 carbon unit shorter. Very interestingly, these fatty acids or their corresponding methylketones caused a strong reduction in, or even a complete inhibition of, citrinin production by M. ruber when they were added to the medium. Several data indicated that this effect could be due to the degradation of the newly synthesized citrinin (or an intermediate in the citrinin pathway) by hydrogen peroxide resulting from peroxisome proliferation induced by medium-chain fatty acids or methylketones. 相似文献
6.
Isolation of uroporphyrin III from Clostridium thermoaceticum 总被引:1,自引:0,他引:1
S S Yang L G Ljungdahl G Lund 《Biochemical and biophysical research communications》1979,90(2):575-581
contains a porphyrin which, based on visible absorption and 1H nmr spectra as well as on chromatographic behavior, has been identified as uroporphyrin III. 相似文献
7.
The trochid gastropod Austrocochlea constricta (Lamarck) shows a variable pattern of shell banding. The concentration of the major shell pigment, uroporphyrin I, has been shown to differ between stripe-classes and, within each stripe-class, between shores. This paper describes the relation between amount of available food and shell pigmentation on six shores.Qualitative and quantitative methods were used to determine the concentrations of chlorophyll pigments in the substratum of each shore. Thin-layer chromatography showed differences in the type of microalgae present. Quantitative, spectrophotometric determinations of the chlorophyll concentrations of acetone extracts of scrapings of the substratum showed significant differences in total chlorophyll between the six shores. A uroporphyrin index gives an estimate of the total amount of uroporphyrin/unit area on each shore, and enables the wet wt/animal, density/area, and proportions of the population in different stripe-classes to be related to the concentrations of uroporphyrin/g shell within the stripe-classes. There was a highly significant linear regression of the uroporphyrin index on total chlorophyll concentration. This provides evidence for the effect of amount of available food on shell pigmentation. The mean concentration of pigment/g shell increased as the chlorophyll content of the substratum increased.A hypothesis is proposed to explain the observed differences in banding-pattern frequencies between shores; this represents the frequency distribution of animals with different concentrations of pigment/g shell as a normal distribution, with increasing mean and standard deviation as the concentration of chlorophyll in the substratum increases. Theoretical distributions are given to show that similar banding pattern frequencies to those observed on the six shores can be produced under this hypothesis. Neither a more complex hypothesis, nor selective nor genetic mechanisms are necessary to explain the observed distributions of banding pattern in Austrocochlea. 相似文献
8.
Biofilm-Specific Cross-Species Induction of Antimicrobial Compounds in Bacilli 总被引:9,自引:1,他引:8
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Liming Yan Kenneth G. Boyd David R. Adams J. Grant Burgess 《Applied microbiology》2003,69(7):3719-3727
An air-membrane surface (AMS) bioreactor was designed to allow bacteria to grow attached to a surface as a biofilm in contact with air. When Bacillus licheniformis strain EI-34-6, isolated from the surface of a marine alga, was grown in this reactor, cells produced antimicrobial compounds which they did not produce when they were grown in shake flask cultures. An unidentified red pigment was also produced by surface-grown cells but not by planktonically grown cells. Glycerol and ferric iron were important for the production of antimicrobial compounds and the red pigment. Release of these secondary metabolites was not due to the onset of sporulation. Cell-free spent medium recovered from beneath the reactor membrane could induce production of antimicrobial compounds and red pigment in shake flask cultures. Neither glycerol nor ferric iron was required for production of these inducer compounds. Spent medium from beneath the membrane of an AMS bioreactor culture of Bacillus subtilis strain DSM10T and Bacillus pumilus strain EI-25-8 could also induce production of antimicrobial compounds and a red pigment in B. licheniformis isolate EI-34-6 grown in shake flask cultures; however, the corresponding spent medium from shake flask cultures of DSM10T and EI-25-8 could not. These results suggest that there is a biofilm-specific cross-species signaling system which can induce planktonically grown cells to behave as if they were in a biofilm by regulating the expression of pigments and antimicrobial compounds. 相似文献
9.
《Journal of Fermentation and Bioengineering》1992,73(1):31-36
Adventitious roots were induced from red beet (Beta vulgaris L. cv. Detroit dark red) by infecting the plant with a soil bacterium, Agrobacterium rhizogenes. Based on analysis of opines which are uniquely produced in transformed hairy roots, the established clone was proved to be a transformed hairy root. In a shake culture of the beet hairy root clone with a liquid medium, it was found that significant amounts of pigments, mainly betanin and vulgaxanthin-I, were released into the medium by the cessation of culture shaking (temporary limitation of oxygen supply). The hairy root cells were capable of propagation even after the cells were subjected to shaking cessation. Repeated-batch culture of the beet hairy root was performed with the cell growth phases for 9 or 10 d and with pigment leakage phases during shaking cessation for 2 d, and more than 20% of the total intracellular pigments were recovered from the culture broth at a culture time of 35 d. The released pigments were confirmed to be substantially identical to those extracted from the hairy root and original plant cells of red beet. 相似文献
10.
Abstract The anaerobic, Gram-positive coccus Staphylococcus aureus ssp. anaerobius and its aerobic mutant MVF-SR, when kept under anaerobic conditions, excreted coproporphyrin (mainly type III) into the medium and enriched uroporphyrin (mainly type I) within the cells.
The rate of porphyrin synthesis stayed practically unaltered when the growth medium was supplemented with 50 μ g/ml 5-aminolevulinic acid (ALA), but was significantly enhanced upon supplementation with hemin (0.5 μ g/ml). When hemin and ALA were given simultaneously, a more than two-fold increase in porphyrin production compared to normal growth medium was observed. These observations indicate a stimulation of porphyrin synthesis in S. aureus by hemin.
An as yet unidentified violet pigment with an intense red-violet fluorescence under UV light ( λ = 366 nm) was found to be present in considerable amounts in cells of S. aureus ssp. anaerobius , whereas the supernatant medium of aerobically grown cells of the mutant MVF-SR contained an equally unidentified blue, non-fluorescing pigment. 相似文献
The rate of porphyrin synthesis stayed practically unaltered when the growth medium was supplemented with 50 μ g/ml 5-aminolevulinic acid (ALA), but was significantly enhanced upon supplementation with hemin (0.5 μ g/ml). When hemin and ALA were given simultaneously, a more than two-fold increase in porphyrin production compared to normal growth medium was observed. These observations indicate a stimulation of porphyrin synthesis in S. aureus by hemin.
An as yet unidentified violet pigment with an intense red-violet fluorescence under UV light ( λ = 366 nm) was found to be present in considerable amounts in cells of S. aureus ssp. anaerobius , whereas the supernatant medium of aerobically grown cells of the mutant MVF-SR contained an equally unidentified blue, non-fluorescing pigment. 相似文献
11.
《The International journal of biochemistry》1994,26(2):255-258
- 1.1. The action of uroporphyrin I on erythrocytic ALA-D activity under dark and light conditions was examined.
- 2.2. Photo and non-photoinactivation of ALA-D induced by uroporphyrin I were observed.
- 3.3. Both effects were dependent on uroporphyrin concentration, temperature and time of exposure of the protein to the porphyrin.
- 4.4. Light-dependent effect of uroporphyrin I is related with the phototoxicity of porphyrins and could be produced by primary amino acid photooxidation followed by secondary cross-linking of the protein.
- 5.5. Light-dependent effect of uroporphyrin I could be ascribed to a direct enzyme inhibition due to binding of the porphyrin to the protein inducing structural changes at or near its active site.
12.
Influence of Environmental Factors and Medium Composition on Vibrio gazogenes Growth and Prodigiosin Production 总被引:1,自引:0,他引:1
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Vibrio gazogenes ATCC 29988 growth and prodigiosin synthesis were studied in batch culture on complex and defined media and in chemostat cultures on defined medium. In batch culture on complex medium, a maximum growth rate of 0.75 h−1 and a maximum prodigiosin concentration of 80 ng of prodigiosin · mg of cell protein−1 were observed. In batch culture on defined medium, maximum growth rates were lower (maximum growth rate, 0.40 h−1), and maximum prodigiosin concentrations were higher (1,500 ng · mg of protein−1). In batch culture on either complex or defined medium, growth was characterized by a period of logarithmic growth followed by a period of linear growth; on either medium, prodigiosin biosynthesis was maximum during linear growth. In batch culture on defined medium, the initial concentration of glucose optimal for growth and pigment production was 3.0%; higher levels of glucose suppressed synthesis of the pigment. V. gazogenes had an absolute requirement for Na+; optimal growth occurred in the presence of 100 mM NaCl. Increases in the concentration of Na+ up to 600 mM resulted in further increases in the concentration of pigment in the broth. Prodigiosin was synthesized at a maximum level in the presence of inorganic phosphate concentrations suboptimal for growth. Concentrations of KH2PO4 above 0.4 mM caused decreased pigment synthesis, whereas maximum cell growth occurred at 1.0 mM. Optimal growth and pigment production occurred in the presence of 8 to 16 mg of ferric ion · liter−1, with higher concentrations proving inhibitory to both growth and pigment production. Both growth and pigment production were found to decrease with increased concentrations of p-aminobenzoic acid. The highest specific concentration of prodigiosin (3,480 ng · mg protein−1) was observed in chemostat cultures at a dilution rate of 0.057 h−1. The specific rate of prodigiosin production at this dilution rate was approximately 80% greater than that observed in batch culture on defined medium. At dilution rates greater than 0.057 h−1, the concentration of cells decreased with increasing dilution rate, resulting in a profile comparable to that expected for linear growth kinetics. No explanation could be found for the linear growth profiles obtained for both batch and chemostat cultures. 相似文献
13.
1. The preparation of a crude extract of Clostridium tetanomorphum containing cobalt porphyrin synthase but little haem-synthase activity is described. 2. The properties of cobalt porphyrin synthase in the clostridial extracts is compared with the properties of a haem synthase present in crude extracts of the yeast Torulopsis utilis. 3. Cobalt porphyrin synthase in extracts of C. tetanomorphum inserts Co2+ ions into the following dicarboxylic porphyrins in descending order of rate of insertion: meso-, deutero- and proto-porphyrins. Esterification renders meso- and deutero-porphyrins inactive as substrates. Neither the tetracarboxylic (coproporphyrin III) nor the octacarboxylic (uroporphyrin III) compounds are converted into cobalt porphyrins by the extract, but the non-enzymic incorporation of Co2+ ions into these two porphyrins is rapid. These extracts are unable to insert Mn2+, Zn2+, Mg2+ or Cu2+ ions into mesoporphyrin. 4. Crude extracts of T. utilis readily insert both Co2+ and Fe2+ ions into deutero-, meso, and proto-porphyrins. Unlike the extracts of C. tetanomorphum, these preparations catalyse the insertion of Co2+ ions into deuteroporphyrin more rapidly than into mesoporphyrin. This parallels the formation of haems by the T. utilis extract. 5. Cobalt porphyrin synthase is present in the particulate fraction of the extracts of C. tetanomorphum but requires a heat-stable factor present in the soluble fraction. This soluble factor can be replaced by GSH. 6. Cobalt porphyrin synthase in the clostridial extract is inhibited by iodoacetamide and to a smaller extent by p-chloromercuribenzoate and N-ethylmaleimide. The haem synthases of T. utilis and Micrococcus denitrificans are also inhibited by various thiol reagents. 相似文献
14.
Farhan M. Said John Brooks Yusuf Chisti 《World journal of microbiology & biotechnology》2014,30(9):2471-2479
The carbon-to-nitrogen (C:N) ratio in the biomass of microfungi tends to be quite different (e.g. 10–15) compared with the C:N ratio in the red pigments (e.g. >20) of the fungus Monascus ruber. Therefore, determining an optimal C:N ratio in the culture medium for maximizing the production of the pigments is important. A culture medium composition is established for maximizing the production of the red pigment by the fungus M. ruber ICMP 15220 in submerged culture. The highest volumetric productivity of the red pigment was 0.023 AU L?1 h?1 in a batch culture (30 °C, initial pH of 6.5) with a defined medium of the following composition (g L?1): glucose (10), monosodium glutamate (MSG) (10), MgSO4·7H2O (0.5), KH2PO4 (5), K2HPO4 (5), ZnSO4·7H2O (0.01), FeSO4·7H2O (0.01), CaCl2 (0.1), MnSO4·H2O (0.03). This medium formulation had a C:N mole ratio of 9:1. Under these conditions, the specific growth rate of the fungus was 0.043 h?1 and the peak biomass concentration was 6.7 g L?1 in a 7-day culture. The biomass specific productivity of the red pigment was 1.06 AU g?1 h?1. The best nitrogen source proved to be MSG although four other inorganic nitrogen sources were evaluated. 相似文献
15.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1976,53(1):151-155
- 1.1. There is no uniform pattern of porphyrin synthesis by whole blood and haemolysates, between the different mammalian species studied.
- 2.2. Coproporphyrin, is the dominant porphyrin synthesised by intact red cells.
- 3.3. Uroporphyrin synthesis increases significantly in the majority of species when the cells are haemolysed.
- 4.4. In the mouse large amounts of protoporphyrin are synthesised by intact red cells which increases further on haemolysis.
- 5.5. The low porphyrin synthesising capacity of cow and sheep red cells is not due to any rate-limiting activity of the enzyme ALA-dehydratase.
- 6.6. The dog has a pattern of porphyrin synthesis and excretion similar to that found in the rabbit, and the possibility exists, that a similar energy-dependent carrier mechanism for movement of uroporphyrin and coproporphyrin across red cell membranes found in the rabbit may be present in this species.
- 7.7. The findings may be of significance in the interpretation of porphyrin excretion patterns in experimental porphyria.
16.
《Enzyme and microbial technology》1987,9(12):721-725
An enzymatic production of l-tryptophan from dl-5-indolylmethylhydantoin by the action of hydantoinase and carbamoylase has been investigated. A strain identified as (Flavobacterium) sp. I-3 isolated from soil was found to form l-tryptophan from dl-5-indolylmethylhydantoin. Cultural conditions for the formation of the l-tryptophan-forming activity were investigated, and the highest activity of 0.81 μmol min−1of l-tryptophan formed per 1 ml of culture broth (hydantoinase, 3.6 μmol min−1of N-carbamoyl-l-tryptophan formed per 1 ml of culture broth; carbamoylase, 0.92 μmol min−1of l-tryptophan formed per 1 ml of culture broth) was obtained. These activities were found to be inducible and intracellular. Optimization of the parameters of the conversion reaction resulted in accumulation of 50 mg of l-tryptophan per 1 ml of cultural broth per day. The conversion yield from dl-5-indolylmethylhydantoin was about 100%. Accumulated l-tryptophan was readily isolated in pure form by ordinary procedures. 相似文献
17.
Dhiman Maitra Jared S. Elenbaas Steven E. Whitesall Venkatesha Basrur Louis G. D'Alecy M. Bishr Omary 《The Journal of biological chemistry》2015,290(39):23711-23724
Hepatic accumulation of protoporphyrin-IX (PP-IX) in erythropoietic protoporphyria (EPP) or X-linked-dominant protoporphyria (XLP) cause liver damage. Hepatocyte nuclear lamin aggregation is a sensitive marker for PP-IX-mediated liver injury. We tested the hypothesis that extracellular or intracellular protoporphyria cause damage to different subcellular compartments, in a light-triggered manner. Three hepatoma cell lines (HepG2, Hepa-1, and Huh-7) were treated with exogenous PP-IX (mimicking XLP extrahepatic protoporphyria) or with the iron chelator deferoxamine and the porphyrin precursor 5-aminolevulinic acid (ALA) (mimicking intracellular protoporphyrin accumulation in EPP). Exogenous PP-IX accumulated predominantly in the nuclear fraction and caused nuclear shape deformation and cytoplasmic vacuoles containing electron-dense particles, whereas ALA+deferoxamine treatment resulted in higher PP-IX in the cytoplasmic fraction. Protein aggregation in the nuclear and cytoplasmic fractions paralleled PP-IX levels and, in cell culture, the effects were exclusively ambient light-mediated. PP-IX and ALA caused proteasomal inhibition, whereas endoplasmic reticulum protein aggregation was more prominent in ALA-treated cells. The enhanced ALA-related toxicity is likely due to generation of additional porphyrin intermediates including uroporphyrin and coproporphyrin, based on HPLC analysis of cell lysates and the culture medium, as well as cell-free experiments with uroporphyrin/coproporphyrin. Mouse livers from drug-induced porphyria phenocopied the in vitro findings, and mass spectrometry of liver proteins isolated in light/dark conditions showed diminished (as compared with light-harvested) but detectable aggregation under dark-harvested conditions. Therefore, PP-IX leads to endoplasmic reticulum stress and proteasome inhibition in a manner that depends on the source of porphyrin buildup and light exposure. Porphyrin-mediated selective protein aggregation provides a potential mechanism for porphyria-associated tissue injury. 相似文献
18.
Formation of red pigment by Monascus purpureus via diauxic growth on glucose and ethanol in submerged culture was optimized based on inoculum preparation and culture medium. A vegetative inoculum was prepared from spores grown on ethanol. The optimized culture medium was low in phosphates, and had an initial pH?of 5.5. The characteristics of Monascus purpureus grown on glucose and on ethanol were compared: the specific consumption rate of glucose (qG) was higher than the specific consumption rate of ethanol (qE), whereas the specific growth rate was greatest with ethanol. The specific production rate of red pigment (pOD) and pigment yield (YOD/s) with glucose was twice that with ethanol. A novel fermentation process was developed with M. purpureus initially grown with controlled ethanol formation, and consumption of the latter during pigment formation. 相似文献
19.
A hyperpigment-producing mutant, R-10847, was derived from Monascus kaoliang F-2 (ATCC 26264) through a series of mutagenesis steps. The mutant produced a large quantity of Monascus pigment when grown in mantou (steamed bread) by solid culture. The mutant produced pigments extracellularly by extruding the pigments outside the cell in a lump together with some viscous substances. The productivity of pigment was about 100-fold greater than that of the wild type. The mutant lost the capability of spore formation, the growth rate decreased, and both the size and quantity of conidia were reduced. The color of the pigment produced by the mutant changed from orange to deep red. 相似文献
20.
Monascus purpureus is a biopigment-producing fungi whose pigments can be used in many biotechnological and food industries. The growth kinetics
of biopigment production were investigated in a liquid fermentation medium in a 5-l stirred tank bioreactor at 30°C, pH 7,
for 8 days with 100 rpm agitation and 1.38 × 105 N/m2 aeration. Thai Monascus purpureus strains TISTR 3002, 3180, 3090 and 3385 were studied for color production, growth kinetics and productivity. Citrinin as
a toxic metabolite was measured from the Monascus fermentation broth. The biopigment productions were detected from fermentation broth by scanning spectra of each strain produced.
Results showed a mixture of yellow, orange and red pigments with absorption peaks of pigments occurring at different wavelengths
for the four strains. It was found that for each pigment color, the color production from the strains increased in the order
TISTR 3002, 3180, 3090, 3385 with 3385 production being approximately 10 times that of 3002. Similar results were found for
growth kinetics and productivity. HPLC results showed that citrinin was not produced under the culture conditions of this
study. The L*, a* and b* values of the CIELAB color system were also obtained for the yellow, orange and red pigments produced from the TISTR 3002,
3180, 3090 and 3385 strains. The colors of the pigments ranged from burnt umber to deep red. 相似文献