Studies on the relation between density of Longidorus elongatus and growth of sugar beet, with supplementary observations on Trichodorus spp

Longidorus elongatus attacks sugar beet on light sandy soils in the West Midlands. Severely damaged plants may die or recover, producing fanged roots. Up to 335 L. elongatus/200 g of soil were found around attacked seedling plants and were often visible to the unaided eye on the roots of freshly lifted plants. Five experiments were made and regressions computed of yield of beet, total numbers of plants and numbers of normal and fanged beet on L. elongatus numbers. Between 24% and 50% of roots per 100 L. elongatus/ 200 g were killed or became fanged. Corresponding figures for loss of plant were between 7.5% and 33%. Estimated loss of yield varied between 0.8 and 7.3 tons (2.0–18.3 t/ha)/acre/100 L. elongatus/200 g, the former where the potential yield was high and the latter where poor growing conditions hindered recovery. Large numbers of Trichodorus occurred in two trial sites and there is some evidence of competition between the two genera. Significant negative regressions for Trichodorus spp. were obtained in one trial suggesting a loss of 12% total and 17% normal roots per 100 Trichodorus spp./200 g.     

Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots

Sporamin, a sweet potato tuberous storage protein, is a Kunitz-type trypsin inhibitor. Its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. To test its potential as an anti-feedant for the beet cyst nematode (Heterodera schachtii Schm.), the sporamin gene SpTI-1 was introduced into sugar beet (Beta vulgaris L.) by Agrobacterium rhizogenes-mediated transformation. Twelve different hairy root clones expressing sporamin were selected for studying nematode development. Of these, 8 hairy root clones were found to show significant efficiency in inhibiting the growth and development of the female nematodes whereas 4 root clones did not show any inhibitory effects even though the SpTI-1 gene was regularly expressed in all of the tested hairy roots as revealed by northern and western analyses. Inhibition of nematode development correlated with trypsin inhibitor activity but not with the amount of sporamin expressed in hairy roots. These data demonstrate that the trypsin inhibitor activity is the critical factor for inhibiting growth and development of cyst nematodes in sugar beet hairy roots expressing the sporamin gene. Hence, the sweet potato sporamin can be used as a new and effective anti-feedant for controlling cyst nematodes offering an alternative strategy for establishing nematode resistance in crops.     

The effect of field margins on the yield of sugar beet and cereal crops

The effect of field margins on the yield of sugar beet, wheat and barley was studied on commercial farms and in a series of field experiments from 1992–1997. There was always a trend of increasing yield from the edge of the field to the centre, with a marked reduction around the ‘tramlines’ and the area where machinery turns. In the studies on commercial farms, headland yield loss varied widely. In sugar beet the headlands yielded 19–41% less than the centre, with a mean reduction of 26%. In cereals the range was 3–19%, with a mean loss of 7%. Headland yield reductions were generally smaller in the field experiments than those found on commercial farms. These headland effects did not move towards the centre of the field when grass margins were planted at the edge of the field; there was no significant effect on the yield of the adjacent crop. The presence of boundary trees had the greatest effect on yield: in the outer 9 m of the field, the area shaded by trees produced 4.4 t ha^-1 of wheat, and the area that was not shaded 8.1 t ha^-1. Turning of machinery also significantly reduced yield, while grazing by rabbits and hares surprisingly had no effect. Following the reform of the Common Agricultural Policy in 1992, the main effect of which was to change from a price support policy to direct payments to producers, farmers in the European Union who produce more than a specified tonnage of ‘eligible crops’ per year, are required to fallow a given percentage of their land (currently 5%), to qualify for Arable Area Payments. Growers can elect to fallow fields on a rotational basis, or permanently. Headland set-aside is a term used to describe strips of set-aside, a minimum of 20 m wide around the edges of fields. In these experiments, the headland effect did not extend beyond 20 m from the field edge. Therefore, particularly in fields with boundary hedges or trees, headland set-aside could effectively remove the poor-yielding area at the field margin.     

The effect of soil fumigation with D-D on the yields of sugar beet and other crops

An experiment in a field where sugar beet in 1965 had suffered from Docking disorder caused by Longidorus attenuatus tested the effect of fumigating the soil with 3741/ha D-D and two amounts of nitrogen fertilizer on different crop sequences between 1966 and 1969. Although severe Docking disorder did not recur in sugar beet, fumigation increased yield in each of the three following years. Yield of barley was increased for 4 yr and of wheat, potatoes and ryegrass for 1 or 2 yr after treatment. All plant parasitic nematodes were controlled by the first fumigation and the numbers of those in unfumigated plots 3 yr after treatment. Fumigation also largely prevented infection of sugar beet by the fungus Helicobasidium purpureum.     

Development and incorporation of microsatellite markers into the linkage map of sugar beet (Beta vulgaris spp.)

A set of informative simple sequence repeat markers has been identified for use in the marker-assisted breeding of Beta vulgaris. Highly enriched small insert genomic libraries were constructed, consisting of 1536 clones (with inserts of between 250–900 bp). Screening the clones with CA, CT, CAA, CATA and GATA nucleotide-repeat probes revealed positive hybridisation to over 50% of the clones. Of these 340 were sequenced. Primer pairs were designed for sequences flanking the repeats and, of these, 57 pairs revealed length polymorphism with 12 Beta accessions. Heterozygosity levels of the SSR loci ranged from 0.069 to 0.809. Heterozygosity levels were found to be similar to those detected employing RFLP probes with the same accessions. Phenetic analysis using the markers, indicated relationships in accordance with known pedigrees. Twenty three of the SSR markers were polymorphic in one or both of two F₂ mapping populations, and were placed relative to a framework of RFLP probes. The markers are distributed over all nine linkage groups of sugar beet. Received: 14 July 1999 / Accepted: 27 October 1999     

The long-term effects of four monocultural regimes on two field populations of the nematodes Xiphinema diversicaudatum and Longidorus spp.

Population density changes of Xiphinema diversicaudatum and several Longidorus spp. were observed under four cultural regimes in long-term field experiments. Numbers of X. diversicaudatum increased under strawberry and, to a lesser extent, ryegrass, but remained more or less unchanged in hop and fallow plots. A mixture of four Longidorus spp. increased in numbers under grass but stayed static, or declined slowly under the other cultural regimes. All changes in population density were slow to occur, taking 3 or 4 yr for differences to become statistically significant. Fluctuations in larval numbers were mainly responsible for overall population changes; numbers of adults showed little difference over 5 yr.     

Distribution and incidence of yellowing viruses in sugar beet crops in Spain from 1990 to 1993

Surveys of the principal yellowing viruses of sugar beet, beet yellows virus (BYV) and beet mild yellowing virus (BMYV) in Spain were carried out from 1990–1993. Beet yellowing viruses were detected in all provinces, although the mean percentages of plants infected with BYV and BMYV were practically zero in the southern zone. Within the northern zone high variations from one province to another could be observed. The mean percentages of plants infected with BYV were higher in the Ebro Valley than in the Duero Valley. Areas infected with BYV were very restricted, while BMYV could be found to a variable extent all over Spain, although the infection levels were lower. The incidence and distribution of these viruses in the Spanish sugar beet crop makes the study and application of control measures for beet yellowing viruses necessary.     

Thidiazuron-induced organogenesis and somatic embryogenesis in sugar beet (Beta vulgaris L.)

Summary Improved in vitro tissue culture systems are needed to facilitate the application of recombinant DNA technology to the improvement of sugar beet germplasm. The effects of N ⁶-benzyladenine (BA) and thidiazuron (TDZ) pretreatment on adventitious shoot and somatic embryogenesis regeneration were evaluated in a range of sugar beet breeding lines and commercial varieties. Petiole explants showed higher frequencies of direct adventitious shoot formation and produced more shoots per explant than leaf lamina explants. TDZ was more effective than BA for the promotion of shoot formation. The optimal TDZ concentrations were 2.3–4.6 μM for the induction of adventitious shoot regeneration. Direct somatic embryogenesis from intact seedlings could be induced by either BA or TDZ. TDZ-induced somatic embryogenesis occurred on the lower surface of cotyledons at concentrations of 0.5–2μM and was less genotype-dependent than with Ba. A high frequency of callus induction could be obtained from seedlings and leaf explants, but only a few of the calluses derived from leaf explants could regenerate to plants via indirect somatic embryogenesis. These results demonstrated that TDZ could prove to be a more effective cytokinin for in vitro culture of sugar beet than BA. Rapid and efficient regeneration of plants using TDZ may provide a route for the production of transgenic sugar beet following Agrobacterium-mediated transformation.     

Responses of sugar beet (Beta vulgaris L.) to drought and nutrient deficiency stress

The responses of two sugar beet genotypes, 24367 (putative droughttolerant) and N6 (putative drought intolerant), to drought and nutrientdeficiency stress were investigated in an attempt to identify reliable andsensitive indicators of stress tolerance. In glasshouse-grown plants of bothgenotypes, relative water content (RWC) of the leaves decreased and leaftemperature increased in response to drought stress. Genotype differences inresponse to drought included leaf RWC, glycine betaine accumulation, alterationof shoot/root ratio and production of fibrous roots. Thus, in comparison to N6,genotype 24367 lost less water from leaves, produced more fibrous roots,produced more glycine betaine in shoots and tap roots and had a much reducedshoot/root ratio in response to withholding water for up to 215 h.The hydraulic conductance and sap flow of sugar beet seedlings grown innutrientculture decreased when subjected to nitrogen deficiency stress. Under nitrogensufficient conditions sap flow was greater in 24367 than in N6. The resultsindicate that genotype 24367 is more tolerant to stresses induced by water andnitrogen deficiency and that increased fibrous root development may be a majorfactor in increasing sap flow via a concomitant enhancement of aquaporinactivity.     

Morpho-physiological responses of sugar beet (Beta vulgaris L.) genotypes to drought stress

The identification of morpho-physiological traits related to drought tolerance and high yield potential is a challenge when selecting sugar beet genotypes with greater tolerance to water stress. In this paper, root morphological parameters, antioxidant systems, leaf relative water content (RWC) and H⁺-ATPase activity as key morpho-physiological traits involved in drought tolerance/susceptibility of sugar beet were studied. Genotypes showing a different drought tolerance index (DTI) but a similar yield potential, under moderate (?0.6 Mpa) and severe (?1.2 MPa) water stress, were selected and their morpho-physiological traits were investigated. The results showed a wide genetic variation in morpho-physiological parameters which demonstrated the different adaptive strategies under moderate and severe drought conditions in sugar beet. In particular, an efficient antioxidant system and redox signalling made some sugar beet genotypes more tolerant to drought stress. The alternative strategy of other genotypes was the reduction of root tissue density, which produced a less dense root system improving the axial hydraulic conductivity. These results could be considered as interesting challenge for a better understanding of the drought tolerance mechanisms in sugar beet.     

Effects on Myzus persicae (Sulz.) and transmission of beet yellows virus of applying certain trace elements to sugar beet

Applications of lithium chloride (LiCl), zinc sulphate (ZnSO₄) or nickel sulphate (NiSO₄) to the roots of sugar-beet plants in the glasshouse encouraged settling on the leaves of adult apterae from a clone of Myzus persicae (Sulz.); conversely, treatment with boric acid (H₂B₂O₇) inhibited aphid settling. Larviposition of M. persicae was increased by NiSO₄ and tin chloride (SnCl₂). Viruliferous M. persicae transmitted beet yellows virus (BYV) more efficiently to plants treated with LiCl or H₂B₂O₇ than to those treated with copper sulphate (CuSO₄), ZnSO₄ or SnCl₂. The sulphate and chloride anions of the applied chemicals appeared to have little effect on M. persicae and virus transmission. It is suggested that applications of trace elements to sugar beet affected M. persicae and virus transmission by changing the concentrations of trace elements in the aphids' diet and by altering the metabolism of the leaf tissues in the host plant.     

Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes

Plant parasitic nematodes impose a severe constraint on plantain and banana productivity; however, the sterile nature of many cultivars precludes conventional breeding for resistance. Transgenic plantain cv. Gonja manjaya (Musa AAB) plants, expressing a maize cystatin that inhibits nematode digestive cysteine proteinases and a synthetic peptide that disrupts nematode chemoreception, were assessed for their ability to resist nematode infection. Lines were generated that expressed each gene singly or both together in a stacked defence. Nematode challenge with a single species or a mixed population identified 10 lines with significant resistance. The best level of resistance achieved against the major pest species Radopholus similis was 84% ± 8% for the cystatin, 66% ± 14% for the peptide and 70% ± 6% for the dual defence. In the mixed population, trial resistance was also demonstrated to Helicotylenchus multicinctus. A fluorescently labelled form of the chemodisruptive peptide underwent retrograde transport along certain sensory dendrites of R. similis as required to disrupt chemoreception. The peptide was degraded after 30 min in simulated intestinal fluid or boiling water and after 1 h in nonsterile soil. In silico sequence analysis suggests that the peptide is not a mammalian antigen. This work establishes the mode of action of a novel nematode defence, develops the evidence for its safe and effective deployment against multiple nematode species and identifies transgenic plantain lines with a high level of resistance for a proposed field trial.     

Variability of cathodic peroxidases in sugar beet (Beta vulgaris L.) cultivars

Eighteen varieties of sugar beet (Beta vulgaris L.), originating from various European countries, were compared in respect of peroxidase variability level. They were cultivated in the same experimental plot. The cultivars differed in ploidy level: one variety was tetraploid, three were diploid and 14 varieties were triploid. The cathodic peroxidase system is controlled by four independent genes, of which only one is polymorphic. Consequently, the varieties were characterised by frequencies of 3 allozymes belonging to one locus. Only one variety proved to be fully monomorphic. Genetic similarities between the cultivars were illustrated by a dendrogram (UPGMA) and show different groups of varieties not related to their ploidy level.     

The effect of soil-applied granular pesticides and irrigation on the yield of sugar beet in fields infested with Trichodorus viruliferus

In field samples taken on a grid pattern during four winters over an 80 ha farm there were many Trichodorus viruliferus (up to 3000/litre soil) in light soils but few in heavier soils. Depth samples taken during the summer showed Trichodorus to be the only nematode genus which was more numerous below plough depth than in the plough layer. In field experiments in the most heavily infested part of the farm, soil-applied granular pesticides did not increase sugar yields following the dry spring of 1982; aldicarb increased sugar yields in one experiment following the wet spring of 1983 although it, and other granular pesticides, did not affect yield in an adjacent experiment where T. viruliferus were fewer. Irrigation during the dry summer of 1983 maintained relatively large populations of T. viruliferus, whereas in unirrigated plots without aldicarb populations declined by over 50%. Economic thresholds for T. viruliferus in 1983 at Broom's Barn were about 700/litre in unirrigated soil and 300/litre in irrigated soil.     

Glasshouse tests and field selection for heritable resistance to Myzus persicae in sugar beet

A technique is described to assess and select in the field for resistance to Myzus persicae in sugar beet. Lines developed by this method were aphid resistant when compared with commercial cultivars. The resistance was inherited by progeny lines. Variation between beet lines in glasshouse tests suggested segregation of a number of resistance genes with varying effects. Integration of selection for aphid-resistance and agronomic performance during beet breeding is discussed.