Macrophyte-epiphyte biomass and productivity in an eelgrass (Zostera marina L.) community

The biomass, productivity (¹⁴C), and photosynthetic response to light and temperature of eelgrass, Zostera marina L. and its epiphytes was measured in a shallow estuarine system near Beaufort, North Carolina, during 1974. The maximum of the biomass (above-ground) was measured in March; this was followed by a general decline throughout the rest of the year. The average biomass was 105.0 g dry wt m^?2; 80.3 g dry wt m^?2 was eelgrass and 24.7 g dry wt m^?2 was epiphytes. The productivity of eelgrass averaged 0.88 mg C g^?1 h^?1 which was similar to that of the epiphytes, 0.65 mg C g^?1 h^?1. Eelgrass and epiphyte productivity was low during the spring and early summer, gave a maximum during late summer and fall, and declined during the winter; this progression was probably due to environmental factors associated with tidal heights. On an areal basis, the average annual productivity was 0.9 g C m^?2 day^?1 for eelgrass and 0.2 g C m^?2 day^?1 for the epiphytes. Rates of photosynthesis of both eelgrass and epiphytes increased with increasing temperature to an asymptotic value at which the system was light saturated. Both eelgrass and epiphytes had a temperature optimum of < 29 °C. A negative response to higher temperatures was also reflected in biomass measurements which showed the destruction of eelgrass with increasing summer temperatures. The data suggest that the primary productivity cycles of macrophytes and epiphytes are closely interrelated.     

Population dynamics and life history adaptations of Littorina neglecta Bean in an eelgrass meadow (Zostera marina L.) in Nova Scotia

The density of Littorina neglecta Bean from an eelgrass meadow in Nova Scotia was highest in mid-summer (July 1979; 45059 per m²) but fell rapidly during late summer and early autumn due to high mortality of new recruits and two year old individuals. Density remained constant until the following spring when it increased, due to movement of some adults into the study area and the release of young by females (up to ≈ 34000 per m²). During most of the year individuals were found on vegetation (live and dead eelgrass and drift algae) but nearly all moved down to the mud surface during winter to avoid ice (up to 25 cm thick) which was present from December to early March. Movement of ice was not a source of mortality for L. neglecta. Size-frequency histograms showed distinct cohorts and it was possible to follow the growth of year classes. Maximum age was 3 yr and maximum shell height was 4.56 mm. Males and females matured when 1 yr old at 1.7 and 1.8 mm shell height respectively. Size-specific fecundity was very high for such a small littorinid. These life history attributes appeared to be related to the low probability of survival to the maximum age. L. neglecta consumed epiphytes and detrital particles from the surface of eelgrass leaves, and the proportion of eelgrass plants with epiphytes was always low. Grazer exclusion experiments showed that L. neglecta controlled the amount of epiphytes and detritus on eelgrass leaves, and the population thus had a limited food supply. There were significant positive relations between surface area of plants and the numbers of adult and 0 + aged L. neglecta during summer and at that time 0 + individuals were restricted to the base of plants where epiphyte biomass was lowest. Post-recruitment mortality was probably related to intraspecific competition for food. Predation by a naticid gastropod probably accounted for much of the mortality of 2 + aged individuals. L. neglecta has a major positive influence on the growth of eelgrass and probably contributes significantly to nutrient recycling in the eelgrass community.     

EPIPHYTE-GRAZER INTERACTIONS ON ZOSTERA MARINA (ANTHOPHYTA: MONOCOTYLEDONES): EFFECTS OF DENSITY ON COMMUNITY FUNCTION1

Interactions between algal epiphytes and their grazers can have a significant impact on the structure and function of eelgrass (Zostera marina L.) meadows. In Puget Sound, the herbivorous gastropod Lacuna variegata Carpenter and its congeners appear to remove large quantities of the epiphytic community from eelgrass blades. When snails at typical field densities were used in microcosms, Lacuna significantly reduced epiphytic biomass and areal productivity. Biomass-specific productivity of the epiphytic community showed an increasing trend with increasing snail density. Epiphytic productivity increased nonlinearly with increasing epiphytic biomass. The commonly used logistic population growth formula adequately described this relationship. Grazing rate also increased nonlinearly with increasing epiphytic biomass. The Hailing equation adequately described the relationship between grazing rate and epiphytic biomass. The proportion of the epiphytic biomass found on the oldest blade of an eelgrass shoot was related linearly to epiphytic biomass, suggesting that a constant fraction of the epiphytic community is lost regardless of epiphytic density. Lacuna clearly removed large quantities of epiphytic material from eelgrass blades, significantly altering community function. Modified Lotka-Volterra equations, incorporating the logistic growth form and Hailing grazing equation, should prove useful in modeling the epiphyte–grazer interaction.     

RESPONSE OF MICROALGAL EPIPHYTE COMMUNITIES TO NITRATE ENRICHMENT IN AN EELGRASS (ZOSTERA MARINA) MEADOW1

The community structure and productivity of epiphytic microalgae on field populations of eelgrass (Zostera marina L.) from a high flow regime were characterized under water-column nitrate enrichment over a 30–d period during the autumn growing season for the macrophyte. Epiphyte communities in replicate low-nitrogen sites (LOW-N, median water-column nitrate concentrations below detection) were compared to communities in replicate N-enriched sites wherein nitrate was leached from clay pots filled with enriched agar (N-ENRICH, median concentration ca. 6 μM NO₃^?_-N; pots replaced at 8– to 12–d intervals). In experimental chambers, total epiphyte community productivity as ¹⁴C-bicarbonate uptake was determined from short-term (3–h) laboratory assays. Track light microscope-autoradiography enabled estimates of species-specific productivity for abundant algal taxa. After 6 d in the LOW-N and N-ENRICH communities, the crustose adnate red alga Sahlingia subintegra (Rosenvinge) Kornmann was dominant in terms of cell number and codominant in biovolume. Photosynthetic dinoflagellates, not previously reported as abundant in eelgrass epiphyte communities, were dominant in biovolume contribution after both 6 and 30 d in LOW-N communities. Nitrate enrichment stimulated the adnate monoraphid diatom Cocconeis placentula Ehr. but apparently inhibited dinoflagellates and the diatom Melosira sp. Total productivity of the epiphyte communities remained comparable in both the LOW-N and N-ENRICH sites. Shifts in community structure and species-specific productivity, however, indicated a controlling influence of nitrate supply on microalgal epiphytes in the field eelgrass community.     

The ecology of eelgrass, Zostera marina (L.), fish communities. I. Structural analysis

Fish populations utilizing eelgrass, Zostera marina (L.), beds in two different estuarine areas near Beaufort, North Carolina were analysed and compared to determine aspects of their community structure. The fish community of the eelgrass beds was characterized by low diversity and high standing crops of biomass and energy, both of which showed seasonal variation. Wide temperature fluctuations related to the overall shallowness of the beds probably regulated the diversity of fishes utilizing the beds. This community was dominated by pinfish, Lagodon rhombiodes (L.), which comprised 45% and 67% of the fish biomass in the Phillips Island and Bogue Sound beds, respectively.Changes in total body caloric content were probably related to developmental stages and changes in diet. Adult fish often had significantly higher weight-specific caloric contents than juvenile fish. Monthly or seasonal variations in caloric content of the organic matter of pinfish had little influence on the caloric content within the various sizes of pinfish.There was a significant correlation between fish biomass, temperature, and Zostera biomass. Fish biomass was highest when temperature and grass biomass were at a maximum. In general, water depth over the beds had little effect on the standing crop of fish within the bed, but cooler waters which occurred at night, darkness, or both, had a large effect.     

The ecology of eelgrass, Zostera marina (L.), fish communities. II. Functional analysis

Consumption, production, and respiration of fish communities utilizing two eelgrass beds in a shallow estuarine system near Beaufort, North Carolina have been estimated for 1971–1972: annual production was 21.7 kcal/m² in each bed with pinfish accounting for 45 and 68% of the production in the Phillips Island and Bogue Sound beds, respectively. Annual community respiration was 57.9 and 69.7 kcal/m² in the two beds with pinfish accounting for 62.6 and 26.7% of the total in the Bogue Sound and Phillips Island beds, respectively. Estimation of the annual food energy consumed by the eelgrass fish community using the Winberg and daily ration methods gave values within 6% of each other.Energy turnover was high (2.8), and the efficiency of energy dissipation low for the two eelgrass fish communities, suggesting that the resident fish populations were adapted to the temperature extremes within the bed. High ecological efficiencies of 0.24 and 0.23 and the high overall efficiency of the eelgrass system (production/solar radiation) of 0.0051 and 0.0086% indicate that the eelgrass beds are efficient systems for converting consumed energy and solar radiation into fish.     

Bay-scale assessment of eelgrass beds using sidescan and video

The assessment of the status of eelgrass (Zostera marina) beds at the bay-scale in turbid, shallow estuaries is problematic. The bay-scale assessment (i.e., tens of km) of eelgrass beds usually involves remote sensing methods such as aerial photography or satellite imagery. These methods can fail if the water column is turbid, as is the case for many shallow estuaries on Canada’s eastern seaboard. A novel towfish package was developed for the bay-scale assessment of eelgrass beds irrespective of water column turbidity. The towfish consisted of an underwater video camera with scaling lasers, sidescan sonar and a transponder-based positioning system. The towfish was deployed along predetermined transects in three northern New Brunswick estuaries. Maps were created of eelgrass cover and health (epiphyte load) and ancillary bottom features such as benthic algal growth, bacterial mats (Beggiatoa) and oysters. All three estuaries had accumulations of material reminiscent of the oomycete Leptomitus, although it was not positively identified in our study. Tabusintac held the most extensive eelgrass beds of the best health. Cocagne had the lowest scores for eelgrass health, while Bouctouche was slightly better. The towfish method proved to be cost effective and useful for the bay-scale assessment of eelgrass beds to sub-meter precision in real time.     

Agent‐based modeling of the effects of forest dynamics,selective logging,and fragment size on epiphyte communities

1. Forest canopies play a crucial role in structuring communities of vascular epiphytes by providing substrate for colonization, by locally varying microclimate, and by causing epiphyte mortality due to branch or tree fall. However, as field studies in the three‐dimensional habitat of epiphytes are generally challenging, our understanding of how forest structure and dynamics influence the structure and dynamics of epiphyte communities is scarce.
2. Mechanistic models can improve our understanding of epiphyte community dynamics. We present such a model that couples dispersal, growth, and mortality of individual epiphytes with substrate dynamics, obtained from a three‐dimensional functional–structural forest model, allowing the study of forest–epiphyte interactions. After validating the epiphyte model with independent field data, we performed several theoretical simulation experiments to assess how (a) differences in natural forest dynamics, (b) selective logging, and (c) forest fragmentation could influence the long‐term dynamics of epiphyte communities.
3. The proportion of arboreal substrate occupied by epiphytes (i.e., saturation level) was tightly linked with forest dynamics and increased with decreasing forest turnover rates. While species richness was, in general, negatively correlated with forest turnover rates, low species numbers in forests with very‐low‐turnover rates were due to competitive exclusion when epiphyte communities became saturated. Logging had a negative impact on epiphyte communities, potentially leading to a near‐complete extirpation of epiphytes when the simulated target diameters fell below a threshold. Fragment size had no effect on epiphyte abundance and saturation level but correlated positively with species numbers.
4. Synthesis: The presented model is a first step toward studying the dynamic forest–epiphyte interactions in an agent‐based modeling framework. Our study suggests forest dynamics as key factor in controlling epiphyte communities. Thus, both natural and human‐induced changes in forest dynamics, for example, increased mortality rates or the loss of large trees, pose challenges for epiphyte conservation.

     

Epiphytic algae on eelgrass at Roscoff,France

Algae growing as epiphytes on eelgrass, Zostera marina L., at Roscoff were investigated during one year by examining the species richness and abundance. The abundance of the algae, expressed as cover values, appeared to be influenced by the characteristics of the substrate, especially the ephemeral status and growth rate of the leaves.Colonization of the seagrass leaves occurred primarily on tips and edges, subsequently spreading over the faces to the base. The pioneers appeared to be the dominant, persistent taxa, of which Audouinella virgatula (Harv.) Dixon, Fosliella lejolisii (Rosan.) Howe, Myrionema magnusii (Sauv.) Lois. and the diatoms were most prominent. The oldest parts of fully grown leaves showed the most diverse epiphytic vegetation. Though there was a form of succession, a climax community was not reached.On examining the spatial distribution of the epiphytes, a group of characteristic taxa was seen to be accompanied by a second group, whose variation in occurrence seemed to be correlated with the height in the littoral.     

Ecology,not distance,explains community composition in parasites of sky-island Audubon’s Warblers

Haemosporidian parasites of birds are ubiquitous in terrestrial ecosystems, but their coevolutionary dynamics remain poorly understood. If species turnover in parasites occurs at a finer scale than turnover in hosts, widespread hosts would encounter diverse parasites, potentially diversifying as a result. Previous studies have shown that some wide-ranging hosts encounter varied haemosporidian communities throughout their range, and vice-versa. More surveys are needed to elucidate mechanisms that underpin spatial patterns of diversity in this complex multi-host multi-parasite system. We sought to understand how and why a community of avian haemosporidian parasites varies in abundance and composition across elevational transects in eight sky islands in southwestern North America. We tested whether bird community composition, environment, or geographic distance explain haemosporidian parasite species turnover in a widespread host that harbors a diverse haemosporidian community, the Audubon’s Warbler (Setophaga auduboni). We tested predictors of infection using generalized linear models, and predictors of bird and parasite community dissimilarity using generalized dissimilarity modeling. Predictors of infection differed by parasite genus: Parahaemoproteus was predicted by elevation and climate, Leucocytozoon varied idiosyncratically among mountains, and Plasmodium was unpredictable, but rare. Parasite turnover was nearly three-fold higher than bird turnover and was predicted by elevation, climate, and bird community composition, but not geographic distance. Haemosporidian communities vary strikingly at fine spatial scales (hundreds of kilometers), across which the bird community varies only subtly. The finer scale of turnover among parasites implies that their ranges may be smaller than those of their hosts. Avian host species should encounter different parasite species in different parts of their ranges, resulting in spatially varying selection on host immune systems. The fact that parasite turnover was predicted by bird turnover, even when considering environmental characteristics, implies that host species or their phylogenetic history plays a role in determining which parasite species will be present in a community.     

In situ phytoremediation of PAH- and PCB-contaminated marine sediments with eelgrass (Zostera marina)

In view of the fact that there are presently no cost-effective in situ treatment technologies for contaminated sediments, a 60-week-long phytoremediation feasibility study was conducted in seawater-supplied outdoor ponds to determine whether eelgrass (Zostera marina) is capable of removing polynuclear aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) from submerged marine sediments. It was determined that all PAHs and PCBs, independent of the number of aromatic rings and degree of chlorination, respectively, were removed to a much larger extent in planted sediments compared to unplanted controls. After 60 weeks of treatment, the concentration of total PAHs decreased by 73% in planted sediments but only 25% in unplanted controls. Similarly, total PCBs declined by 60% in the presence of plants while none were removed in the unplanted sediment. Overall, the apparent PAH and PCB biodegradation was greatest in the sediment layer that contained most of the eelgrass roots. Abiotic desorption tests conducted at week 32 confirmed that the phytoremediation process was not controlled by mass-transfer or bioavailability limitations since all PAHs and PCBs desorbed rapidly and to a large extent from the sediment. PAHs were detected in both roots and shoots, with root and shoot bioaccumulation factors for total PAHs amounting to approximately 3 and 1, respectively, after 60 weeks of phytoremediation treatment. Similarly, the root bioaccumulation factor for total PCBs was around 4, while no PCBs were detected in the eelgrass leaves at the end of the experiment. The total mass fraction of PAHs and PCBs absorbed and translocated by plant biomass during the 60-week period was insignificant, amounting to less than 0.5% of the total mass of PAHs and PCBs which was initially present in the sediment. Finally, the number of total heterotrophic bacteria and hydrocarbon degraders was slightly but not statistically significantly greater in planted sediments than in unplanted controls. After ruling out contaminant loss to the water column or absorption and transformation within plant cells, it is most likely that the presence of eelgrass stimulated the microbial biodegradation of PAHs and PCBs in the rhizosphere by releasing root exudates, plant enzymes, or even oxygen. Additional research is needed to further elucidate these potential phytoremediation mechanisms.     

Glucose Metabolism in Sediments of a Eutrophic Lake: Tracer Analysis of Uptake and Product Formation

The uptake of glucose and the formation of end products from glucose catabolism have been measured for sediments of eutrophic Wintergreen Lake with a combination of tritiated and ¹⁴C-labeled tracers. Time course analyses of the loss of [³H]glucose from sediments were used to establish rate constants for glucose uptake at natural substrate concentrations. Turnover times from these analyses were about 1 min for littoral and profundal sediments. No seasonal or site differences were noted in turnover times. Time course analyses of [U-¹⁴C]glucose uptake and ¹⁴C-labeled end product formation indicated that glucose mass flow could not be calculated from end product formation since the specific activity of added [¹⁴C]glucose was significantly diluted by pools of intracellular glucose and glucose metabolites. Mass flow could only be accurately estimated by use of rates of uptake from tracer studies. Intermediate fermentation end products included acetate (71%), propionate (15%), lactate (9%), and only minor amounts of butyrates or valerates. Addition of H₂ to sediments resulted in greater production of lactate (28%) and decreased formation of acetate (50%), but did not affect glucose turnover. Depth profiles of glucose uptake indicated that rates of uptake decreased with depth over the 0- to 18-cm interval and that glucose uptake accounted for 30 to 40% of methanogenesis in profundal sediments.     

Some structural and functional aspects of the epiphytic component of four seagrass species (Cymodoceoideae) from Papua New Guinea

The epiphytic component of four monospecific seagrass beds from Papua New Guinea was studied structurally and functionally. The floristic composition and abundance of the epiphytes on leaves of four seagrass species (Cymodoceoideae) showed considerable variation, but on all four seagrass species, the same algae were among the five quantitatively most important epiphytes: encrusting coralline algae, Cyanophyta, Ceramium gracillimum (Harv.) Mazoyer, Polysiphonia savatierii Hariot and Audouinella spp. The temporal pattern of the epiphytic algae showed more or less the same features on the four seagrass species.Annual mean biomass of epiphytes and seagrass leaves ranged from 54 g ADW m^?2 in a community of Cymodocea rotundata Ehrenb. and Hempr. ex Aschers. to 169 g ADW m^?2 in a community of Syringodium isoetifolium (Aschers.) Dandy. The contribution of the epiphytic component to the total above-ground biomass ranged from 22 to 24%. Productivity of epiphytes was highest on leaves of Halodule uninervis (Forssk.) Aschers. (2.12 g ADW m^?2 sediment surface day^?1) and the epiphytic community contributed 35–44% of the total above-ground production of these four seagrass communities.     

Influence of Habitat and Climate Variables on Arbuscular Mycorrhizal Fungus Community Distribution,as Revealed by a Case Study of Facultative Plant Epiphytism under Semiarid Conditions

In semiarid Mediterranean ecosystems, epiphytic plant species are practically absent, and only some species of palm trees can support epiphytes growing in their lower crown area, such as Phoenix dactylifera L. (date palm). In this study, we focused on Sonchus tenerrimus L. plants growing as facultative epiphytes in P. dactylifera and its terrestrial forms growing in adjacent soils. Our aim was to determine the possible presence of arbuscular mycorrhizal fungi (AMF) in these peculiar habitats and to relate AMF communities with climatic variations. We investigated the AMF community composition of epiphytic and terrestrial S. tenerrimus plants along a temperature and precipitation gradient across 12 localities. Epiphytic roots were colonized by AMF, as determined by microscopic observation; all of the epiphytic and terrestrial samples analyzed showed AMF sequences from taxa belonging to the phylum Glomeromycota, which were grouped in 30 AMF operational taxonomic units. The AMF community composition was clearly different between epiphytic and terrestrial root samples, and this could be attributable to dispersal constraints and/or the contrasting environmental and ecophysiological conditions prevailing in each habitat. Across sites, the richness and diversity of terrestrial AMF communities was positively correlated with rainfall amount during the most recent growing season. In contrast, there was no significant correlation between climate variables and AMF richness and diversity for epiphytic AMF communities, which suggests that the composition of AMF communities in epiphytic habitats appears to be largely determined by the availability and dispersion of fungal propagules from adjacent terrestrial habitats.     

The role of hydrodynamics in recruitment,growth, and survival of Argopecten irradians (L.) and Anomia simplex (D'Orbigny) within eelgrass meadows

Larvae of the bay scallop Argopecten irradians (L.) and the common jingle Anomia simplex (D'Orbigny) use byssal threads to settle onto blades of eelgrass Zostera marina (L.) and other elevated substrata. A 2-yr study of the role of hydrodynamics in recruitment of these bivalves into eelgrass meadows, and in their subsequent growth and survival as juveniles, was conducted in Northwest Harbor, New York. Sea-water flux through the meadow varied significantly among sites and depended on the numerical density of eelgrass shoots. Recruitment of both bivalves varied significantly among sites in a similar manner, indicating that hydrodynamics in eelgrass meadows strongly affects bivalve recruitment. Results from experimental deployments of two types of substrata indicate that hydrodynamics in eelgrass meadows exerts a stronger influence on bivalve recruitment than does either interblade abrasion or possible differences among sites in the nature of the blade substratum. In one of the two study years, hydrodynamics in eelgrass meadows influenced growth and survival of Argopecten following settlement. Abundances and growth of Anomia following settlement appeared unaffected by hydrodynamics. Several lines of evidence suggest that predation is less important than hydrodynamics in determining abundances of recruits and early juvenile stages of these bivalves.     

Epiphytes on the rocky intertidal red alga RhodomelaLarix (Turner) C. Agardh: Negative effects on the host and food for herbivores?

The diversity of epiflora and fauna associated with a dominant turf-forming alga was examined in intertidal communities on the central Oregon coast. Epiphytes associated with the red alga, Rhodomelalarix (Turner) C. Agardh, were examined by surveying intertidal areas for the presence of epiphytes, and by following changes in epiphyte cover in marked quadrats of R. larix. The alga is host for at least 17 species of sessile plants and animals. To determine the role of some of the larger epiphytes in the community, Rhodomela plants were marked and monitored and herbivore feeding was examined. Data suggest that epiphytes decrease the growth rate of their host, increase the probability of axis breakage and decrease reproductive output. Epiphytes provide food for littorine snails and gammarid amphipods that live in the beds of the plant. Amphipods were found to decrease epiphyte cover on R. larix in laboratory tanks, suggesting that these herbivores may have beneficial effects on the host plant.     

Small-scale removal of seagrass (Zostera marina L.): effects on the infaunal community

Eelgrass meadows are a common feature in shallow waters along the Norwegian coast, where they provide a habitat for a diverse infaunal community. Recreational boat anchoring and moorings physically scour seagrass and may affect the ecosystem functioning and resilience of the system to natural and anthropogenic disturbances. A small-scale eelgrass (Zostera marina) removal experiment was conducted to study the effects on macro- and meiofauna. Entire plants, including the rhizomes, were removed from 4?m² patches in three eelgrass meadows in the inner Oslofjord in October 2010. Core samples were taken after a recovery period of 10 months, from the removed patches as well as from the surrounding meadow. Macrofauna (>500?μm) and meiofauna (63–500?μm) in the sediment were investigated for possible effects of the eelgrass removal. Macrofauna and meiofauna composition were site specific and therefore location was identified as the main determinant for the infaunal community. The eelgrass did not regrow within the recovery period and bare sediment patches with only single eelgrass shoots were present during the sampling. Our analyses support an influence of the removal on individual species, but not the complete community. In particular one species, the gastropod Peringia ulvae, was encountered in higher numbers in samples from the removed patches than in control samples. From a management perspective, such minor removal of eelgrass, on the scale of square metres, appears to have no long-lasting detrimental effect to the infaunal community in sheltered meadows with muddy sediments.     

Experimental test of biodeposition and ammonium excretion from blue mussels (Mytilus edulis) on eelgrass (Zostera marina) performance

Blue mussels and eelgrass have been found to coexist in many locations. However, knowledge of the interactions between these species is limited. Two experiments were conducted in the laboratory, a “Deposit” and an “Epiphyte” experiment. The Deposit experiment examined possible effects of increasing load of blue mussel (Mytilus edulis) biodeposits on sediment biogeochemistry and eelgrass (Zostera marina) performance. Z. marina mesocosms received normal or high loads of mussel biodeposits (Normal and High), while no biodeposits were added to the Control. High dosage had overall negative effects on Z. marina, which was reflected as lower leaf numbers and biomass and accumulation of elemental sulphur in rhizomes. The sediment biogeochemical conditions were altered, as the mussel biodeposits enhanced sulphate reduction rates and increased sulphide concentrations in the porewater, which may result in sulphide invasion and reduced growth of Z. marina.In the Epiphyte experiment effects of mussel excretion, with particular emphasis on ammonium, on the growth of Z. marina and their epiphytes were examined. A thick cover of epiphytes developed on Z. marina growing together with M. edulis, and the relative growth rate was reduced with 20% compared to plants from control without mussels. Overall the experiments showed negative effects on Z. marina growing together with M. edulis, thereby supporting a preceding field study by Vinther et al. [Vinther, H.F., Laursen, J.S., Holmer, M. 2008. Negative effects of blue mussel (Mytilus edulis) presence in eelgrass (Zostera marina) beds in Flensborg fjord, Denmark. Est. Coast Shelf. Sci. 77, 91-103.].     

Leaf vs. epiphyte nitrogen uptake in a nutrient enriched Mediterranean seagrass (Posidonia oceanica) meadow

In situ nitrogen uptake by leaves and epiphytes was studied in a Mediterranean seagrass (Posidonia oceanica) meadow impacted from a fish farm and a pristine meadow, using ¹⁵NH₄ and ¹⁵NO₃ as tracers. In the impacted meadow both leaves and epiphytes yielded higher N concentrations and showed higher specific N uptake, suggesting a linkage between N uptake and its accumulation. Epiphytes took up N faster than leaves in relation to their corresponding biomass, but when assessed per unit area, N uptake was higher in leaves. Leaf N uptake was negatively correlated with epiphyte N uptake. With increasing epiphyte load on leaves, N leaf uptake decreased while N epiphyte uptake increased, indicating that epiphyte overgrowth hinders N uptake by P. oceanica leaves. Epiphyte contribution to total N uptake increased, while that of leaves decreased at the impacted meadow. However, 2-3 times less N was transferred daily from the water column to the benthic compartment, through seagrass and epiphyte uptake on total, at the impacted meadow. Therefore, it is probably still the loss of the key species - the seagrass - which plays the most important role in N cycling in seagrass ecosystems.