Lipid Constituents of Callus Tissues of Mentha spicata

A callus tissue culture was established from leaves and stems of Mentha spicata L. Lipid constituents isolated from callus tissues were composed of fatty acids, fatty acid methyl esters, triglycerides, squalene, stigrnasterol, sitosterol, oleanolic acid (1), ursolic acid (2) and pomolic acid (3). These liquid constituents did not contain monoterpenoids.     

Comparative Studies of Myosin Rods Isolated by Enzymatic and Non-enzymatic Cleavage of Myosin

Tryptic (LMM Fr 1) and CNBr-treated (LMM-C) rod portion of myosin molecule were prepared from rabbit skeletal muscle myosin. An attempt was made to clarify the difference between LMM Fr 1 and LMM-C as measured by solubility and some physico-chemical techniques. The rigid structure of LMM Fr 1 melted into a random coil as temperature of the solution increased and the value of b₀ and reduced viscosity did not show full recovery upon gradual cooling. The situation of LMM-C, on the other hand, showed the higher thermostability than LMM Fr 1. As stated in our previous paper,¹⁾ we have considered that the rod portion of myosin molecules is substantially thermostable.     

Derepression of FAD Pyrophosphorylase and Flavin Changes during Growth of Kloeckera sp. No. 2201 on Methanol

A methanol-utilizing yeast Kloeckera sp. No. 2201, when grown with methanol as a sole carbon and energy source, accumulated about three times much flavin as those grown with glucose, ethanol, or glycerol. A high proportion of the total flavin was FAD in methanol-grown cells. A remarkable derepression of FAD pyrophosphorylase accompanied by an inducible formation of an FAD-dependent alcohol oxidase which catalyzes oxidation of methanol, the first step in the oxidation sequence, was observed during growth of the yeast on methanol. Significant elevations of riboflavin synthetase and flavokinase were also found. Formate, as well as methanol, effectively induced both FAD pyrophosphorylase and methanol-oxidizing enzymes (alcohol oxidase, formaldehyde dehydrogenase, formate dehydrogenase, and catalase). Observations with other methanol-utilizing yeasts also gave essentially same results. These results led to the conclusion that cellular flavin level might be under control with level of flavoprotein physiologically required.     

大肠杆菌RNA聚合酶σ亚基基因的表达及表达产物的分离纯化

将2种表达质拉pEGMD（含rpoD基因）和pETF（含rpoS基因）转化到BL21（DE3）菌株中,培养菌体。破碎细胞,用盐酸抓溶解包含体,经DEAE－纤维素柱层析,SDS－PAGE检测可得纯化σ的蛋白。通过紫外分光光度法测定蛋白浓度,实验表明σ38蛋白的得率为菌体温重的0．27％,σ70蛋白的得率为菌体湿重的0．06％。