共查询到17条相似文献,搜索用时 62 毫秒
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眼镜蛇毒因子(cobra venom factor,CVF)系眼镜蛇毒中的一种蛋白质。近年来,CVF的理化性质、分子生物学特性、免疫学作用以及基因序列等已逐渐被揭示[1,2]。随着对CVF的研究越来越深入,其研究价值越来越广泛,特别是在临床应用方面,正日益引起广大学者的重视。本文对CVF的临床应用现状作一简要综述。 相似文献
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眼镜蛇毒抗风湿性关节炎的药理研究 总被引:1,自引:0,他引:1
本文报导了眼镜蛇毒抗风湿性关节炎实验研究,对大鼠佐剂性关节炎所致的足肿胀有明显的预防和治疗作用;对治疗鸡蛋清、甲醛所致的大鼠关节炎疗效显著,与阳性对照无显著差异;对抑制大鼠棉球肉芽肿的生长中剂量组治疗效果优于阳性对照,与对照组相比(P<0.001);且有降低毛细血管通透性作用(P<0.001)。 相似文献
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目的观察中华眼镜蛇伤后不同时期应用抗蛇毒血清对机体保护作用的差异,为探索临床抗蛇毒血清使用的最佳有效时段提供依据。方法将SD大鼠随机分成6组(蛇毒组、40、60、80、100、120 min血清保护组),每组30只。动物经戊巴比妥腹麻,于单侧背部皮下及双侧小腿腓肠肌注射眼镜蛇毒以制备中华眼镜蛇毒挑战剂量(4×LD50)大鼠模型,分5个不同时段分别注射抗蛇毒血清,于注毒后连续观察3 h,统计各组平均存活时间、成活率及保护率。结果蛇毒组大鼠注入挑战剂量的眼镜蛇毒后,平均存活时间为(148.8±11.4)min,成活率仅为20%;其他血清保护组分别于注毒后40、60、80、100、120 min经腹腔注射精制抗眼镜蛇毒血清(125 u血清/mg蛇毒),40 min血清组保护率达80%;60 min血清组存活率及保护率分别达到70%、50%,平均存活时间为(172.8±7.2)min,与蛇毒组相比有明显差异(P<0.01);而801、00 min血清组保护率依次下降,分别为40%、30%,但仍较蛇毒组显著提高(P<0.01);120 min血清组存活时间及保护率与蛇毒组比较差异无显著性。结论利用中华眼镜蛇毒挑战剂量大鼠模型,通过不同时段施予同剂量抗血清,可显示出明显的机体保护时效性,该研究为探讨临床正确使用抗蛇毒血清提供了新的实验依据。 相似文献
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鸡血清与卵黄中抗中华眼镜蛇毒IgY动态变化研究 总被引:2,自引:0,他引:2
目的探索特异性IgY的产生和变化规律。方法用眼镜蛇毒原毒免疫产蛋母鸡,ELISA定期检测卵黄中的抗体效价变化,小鼠体外中和实验检测其生物活性。第1次免疫40周后,眼镜蛇毒攻击已免疫母鸡,检测攻击前后鸡血清中抗体效价变化情况,未经眼镜蛇毒免疫的母鸡作阴性对照。结果经免疫后第7天蛋黄中即可检测到抗体,经多次加强免疫,40周时蛋黄中还能保持高效价的抗体,通过分离纯化,此抗体可保护实验小鼠免受4 LD50眼镜蛇毒的攻击;同时,鸡血清中也保留着较高效价的抗体,可中和4 LD50以上的眼镜蛇毒。结论用眼镜蛇毒免疫鸡,经多次加强免疫,卵黄和鸡血清中可持久保持高效价的特异性抗体,初步检测此抗体可中和4 LD50的蛇毒。 相似文献
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Comparative Study of Structure and Activity of Cytotoxins from Venom of the Cobras Naja oxiana, Naja kaouthia, and Naja haje 总被引:2,自引:0,他引:2
Feofanov AV Sharonov GV Dubinnyi MA Astapova MV Kudelina IA Dubovskii PV Rodionov DI Utkin YN Arseniev AS 《Biochemistry. Biokhimii?a》2004,69(10):1148-1157
Cytotoxins are positively charged polypeptides that constitute about 60% of all proteins in cobra venom; they have a wide spectrum of biological activities. By CD spectroscopy, cytotoxins CT1 and CT2 Naja oxiana, CT3 Naja kaouthia, and CT1 and CT2 Naja haje were shown to have similar secondary structure in an aqueous environment, with dominating beta-sheet structure, and to vary in the twisting angle of the beta-sheet and the conformation of disulfide groups. Using dodecylphosphocholine micelles and liposomes, CT1 and CT2 Naja oxiana were shown to incorporate into lipid structures without changes in the secondary structure of the peptides. The binding of CT1 and CT2 Naja oxiana with liposomes was associated with an increase in the beta-sheet twisting and a sign change of the dihedral angle of one disulfide group. The cytotoxins were considerably different in cytotoxicity and cooperativity of the effect on human promyelocytic leukemia cells HL60, mouse myelomonocytic cells WEHI-3, and human erythroleukemic cells K562. The most toxic CT2 Naja oxiana and CT3 Naja kaouthia possessed low cooperativity of interaction (Hill coefficient h = 0.6-0.8), unlike 10-20-fold less toxic CT1 and CT2 Naja haje (h = 1.2-1.7). CT1 Naja oxiana has an intermediate position on the cytotoxicity scale and is characterized by h = 0.5-0.8. The cytotoxins under study induced necrosis of HL60 cells and failed to activate apoptosis. The differences in cytotoxicity are supposed to be related not with features of the secondary structure of the peptides, but with interactions of side chains of variable amino acid residues with lipids and/or membrane proteins. 相似文献
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目的为了探索乙醇对眼镜蛇毒毒性的影响。方法将眼镜蛇毒不同浓度致死量经不同浓度乙醇体外处理后,分别于小白鼠皮下注射、口服,将致死量蛇毒皮下注射后的小白鼠立即于局部注射乙醇,观察蛇毒毒性情况。结果小白鼠经皮下注射致死量眼镜蛇毒后,在局部注射50%(或异蛇米酒)、75%乙醇0.1~0.2ml有一定的保护作用;口服100倍皮下注射致死量眼镜蛇毒未发现有毒性表现,口服经50%乙醇处理后的眼镜蛇毒(100倍皮下注射致死量)未增加小鼠死亡率。结论眼镜蛇毒体外经过乙醇处理后毒性有所下降。口服少量的眼镜蛇毒是安全的。眼镜蛇毒与乙醇混合后口服未见蛇毒毒性增加。 相似文献
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目的 蛇毒含有多种生物活性成分, 从天然蛇毒中提取分离蛇毒有效成分受到蛇毒资源和质量的限制。为开发蛇毒有效成分的基因工程产品, 本研究构建了蛇毒腺的c D N A 文库, 为进一步筛选、克隆和表达蛇毒有关基因做准备。 方法 从眼镜蛇( Naja naja atra )毒腺中提取m R N A,经反转录合成c D N A后, 以λgt10 噬菌体为载体, 构建非表达的c D N A 文库。 结果 蛇毒腺c D N A 非表达文库库容量为2×106pfu/μg 重组子, 经大肠杆菌 C600 hlf 菌株平皿测定, 重组率为 70% 。 结论 经平板鉴定和 P C R 快速鉴定表明, 所构 c D N A 文库达到建库要求, 能够用于目的基因筛选和克隆表达。 相似文献
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Armen A. Galoyan Naser Khalaji Lilja E. Hambardzumyan Larisa P. Manukyan Irina B. Meliksetyan Vergine A. Chavushyan Vaghinak H. Sarkisian John S. Sarkissian 《Neurochemical research》2010,35(11):1747-1760
We tested the action of proline-rich peptide (PRP-1) and cobra venom Naja Naja Oxiana (NOX) on Deiters’ nucleus neurons at
3rd, 15th and 35th days after unilateral labyrinthectomy (UL). Early and late tetanic, post-tetanic potentiation and depression
of Deiters’neurons to bilateral high frequency stimulation of hypothalamic supraoptic and paraventricualar nuclei was studied.
The analysis of spike activity was carried out by mean of on-line selection and special program. The complex averaged peri-event
time and frequency histograms shows the increase of inhibitory and excitatory reactions of Deiters’ neurons at early stage
of vestibular compensation following PRP-1 and NOX injection, reaching the norm at the end of tests. In histochemical study
the changes in Ca2+-dependent acidic phosphatase (AP) activity in neurons was discovered. It was shown that in UL animals the total disappearance
or delay of decolorizing of Deiters’ neurons lead to neurodegenerative pattern as cellular “shade”. AP activity after UL and
PRP-1 injection exerts more effective recovery of neurons in comparison with events, observed after the administration of
NOX. The data of this study indicate that PRP-1 and NOX are protectors, which may successfully recover the disturbed vestibular
functions. 相似文献
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