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无花果曲霉原生质体形成与再生条件的探讨 总被引:7,自引:1,他引:6
根据正交试验得出无花果曲霉原生质体形成的最佳条件,用1%的混合酶液(0.5%纤维素酶+0.25%蜗牛酶+0.25%溶菌酶)作用无花果曲霉菌体细胞,原生质体产量达3.2×107个·ml-1,渗透压稳定剂为0.6mol·L-1KCl于0.2mol·L-1PO3+4(pH5.8)中,酶解时间和酶解温度分别为3.0h、30℃.比较不同酶解时间、再生稳定剂和碳源等因素对原生质体再生的影响,可确定最佳再生条件,再生率达30%以上. 相似文献
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角质蛋白酶固态发酵工艺及酶解条件的研究 总被引:2,自引:0,他引:2
曲霉A28-8是一株优良的角蛋白酶分泌突变菌株。其最佳固体发酵培养基为:20%羽毛粉,80%麸皮和微量无机氮(每克培养基中加0.5mg);最适产酶条件为:起始pH7.5~8.0,温度28℃~30℃,时间为60~70小时,酶活高达2500KU/g曲;最适酶解条件为pH7.0~9.0,温度45℃~50℃。 相似文献
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米曲霉原生质体融合及杂合二倍体的形成 总被引:19,自引:1,他引:18
采用混合酶液处理纤维素酶高产苗株3042N-2(天冬酰胺缺陷型“Asn-”)及蛋白酶高产菌株3042N-19(蛋氨酸缺陷型“Met-”)的营养菌丝,获得原生质体,以PEG为助融剂进行融合处理,成功地获得了米曲霉(Aspergillusoryzae)原生质体的营养互补融合。将异核体菌落的菌丝转接到合有0.1%樟脑的新鲜MM上,25℃诱导培养7─15天,挑取绿色角变菌落的孢子,将其转接到MM上能迅速生长,经孢 相似文献
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辣椒子叶原生质体分离条件的研究 总被引:5,自引:0,他引:5
以不同基因型的辣椒子叶为供体组织进行辣椒原生质体分离条件的研究,结果表明:幼龄子叶的原生质体产量与活力均高于老龄子叶;酶解过程中酶液渗透压、酶液浓度、酶解时间均对原生质体分离效果产生重要影响。对于辣椒子叶原生质体,最佳分离条件为酶液甘露醇浓度0.5mol/L,纤维素酶Cellulase Onzuka R-10 1.5,果胶酶Macerozyme R-10 0.6%,酶解时间8-10h。不同基因型辣 相似文献
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芽孢杆菌M_(50)产生β甘露聚糖酶的条件研究 总被引:3,自引:0,他引:3
从土壤中分离到9 株产生β甘露聚糖酶的芽孢杆菌( Bacillus sp .) 。Bacillussp . M50250m L三角瓶摇瓶培养试验,以4 % 的魔芋粉为碳源,1-0 % ( NH4)2SO4 为氮源,0-35 %Na2CO3 ,30 ~34 ℃培养60h 产酶达到高峰。酶活力为180 ~200u/m L。100L 罐发酵,在30 ~32 ℃,1∶0 .75vvm 通气量,200r/min 条件下,发酵液酶活力高达330u/m L。酶的最适反应温度和pH 分别为50 ℃和6-0 ,低于50 ℃,pH5 .0 ~7 .0 酶稳定。Fe3+ 、Al3+ 、EDTA、Hg2+ 对酶有抑制作用,而Ba2+ 、Mn2+ 对酶有激活作用。发酵粗酶液对苎麻精干麻精练,显示对精干麻的半纤维素残胶具有降解作用。 相似文献
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灵芝原生质体分离与再生研究 总被引:29,自引:1,他引:28
首次详细,系统地探讨了灵芝原生质体分离与再生的条件,结果表明,用0.6mol/L甘露醇配制成含2%溶壁酶和0.5%崩溃酶的复合醇,在30℃,pH为6.0时酶解3小时,可得到最高的原生质体得率,但考虑到原生质体的再生,以酶解2.5小时为最适。 相似文献
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李耿光 《植物生理与分子生物学学报》1996,(3)
向日葵籽苗下胚轴原生质体,培养在含有BA0.5mg/L,2,4-D0.5mg/L,NAA0.1mg/L和葡萄糖0.55mg/L的改良Kao培养基中,24~28h后,原生质体开始分裂。包埋在琼脂糖0.6%中的原生质体,培养5d后,分裂频率达95%以上。生长旺盛的小愈伤组织转移到含有2ip0.1mg/L,IAA0.01mg/L,腺嘌呤40mg/L和GA30.01mg/L的Thompson液体培养基上13d后,原生质体诱导的少数愈伤组织发生根分化。 相似文献
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H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
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In this article, we systematically review Dr. Wu Lien-Teh's academic achievements and outstanding contributions in the prevention and control of the plague epidemic in northeast China and introduce the development of the earliest public health epidemic prevention system in China in order to commemorate the 140th anniversary of Dr. Wu Lien-Teh's birth. We hope that this article will provide insights into the effective prevention and control of emerging infectious diseases as well as the current worldwide pandemic of COVID-19, facilitating the improvement and development of public health systems in China and around the globe. 相似文献
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