湖南省健康人群血清总蛋白、白蛋白参考值调查

目的：本实验旨在建立湖南省健康人群血清总蛋白（TP）、血清白蛋白（ALB）及A/G比值的参考值区间。方法：收集4543例湖南省城乡健康参考个体,采用全自动生化分析仪测定其血清TP、ALB值,并计算A/G比值,按不同年龄、性别、城乡进行分组统计学分析,建立适合湖南省人群TP、ALB及A/G比值的参考值。结果：湖南省健康人群参考区间为TP：66.51～82.85 g/L,ALB：43.09～50.19g/L,A/G比值为1.05～2.19;TP随年龄增大而稍有升高,中年达到高峰,后随年龄增大而降低,在各年龄组,女性均高于男性（P〈0.05）,乡村地区TP值高于城市地区（P〈0.05）;ALB随年龄增大稍有升高,在青壮年到达高峰而后下降,在儿童青少年、青壮年组血清ALB男性高于女性（P〈0.05）,而在中年、老年组血清ALB无差别,乡村地区ALB值高于城市地区（P〈0.05）;A/G比值随年龄的变化存在一定的差异性,A/G比值随年龄增大稍有增高,在青壮年组达到高峰而后下降,且在各年龄组男性高于女性（P〈0.05）,城市地区A/G值高于乡村地区（P〈0.05）;湖南省健康人群的TP、ALB与全国其他省市的TP、ALB存在差异。结论：湖南省健康人群TP、ALB、A/G比值参考区间与全国其它省份稍有差异,血清TP、Alb、A/G比值存在年龄、性别和地区差异性。     

肠道菌群正常参考值的检测

目的定性定量检测并比较杭州地区健康青壮年及老年人群肠道主要菌群(双歧杆菌属、类杆菌属、消化链球菌属、产气荚膜梭菌、乳杆菌属、肠杆菌科、肠球菌属及酵母菌等)的正常参考值,同时计算并比较双歧杆菌属细菌数量与肠杆菌科细菌数量的对数值比值(B/E值).方法采用光冈法 .结果老年组肠道双歧杆菌数量及B/E值较青壮年组均显著减少(P＜0.05, P＜0.001);消化链球菌、肠杆菌科及肠球菌细菌数量显著增加(P＜0.01,P ＜0 .001,P＜0.05).结论在获得肠道菌群正常参考值的同时,发现随着年龄的增加,老年人肠道双歧杆菌数量明显减少,肠杆菌科及肠球菌细菌数量显著增加.     

北京市老年人肝肾功能参数的参考值范围调查

目的:观察北京市60岁以上不同年龄段健康老年人肝肾功能检验项目水平的差异,建立各自的参考值范围。方法:随机挑选600例60岁以上的北京市健康老人,按照不同年龄段分为三组,空腹采血,采用Modular仪器及原装试剂测定血清ALT(丙氨酸氨基转移酶)、AST(天冬氨酸氨基转移酶)、TP(总蛋白)、ALB(白蛋白)、Bun(尿素氮)、Crea(肌酐)、UA(尿酸)水平,结果利用SPSS11.0进行统计分析,根据统计学结果,判断参考值范围,并比较不同组间水平的差异。同时随机选择371例健康青年,与老年组进行这些项目水平的比较。结果:统计学结果显示,AST、TP、UA组间无显著差异;ALT、ALB随年龄增加而下降;Bun、Crea随年龄增加而升高。结论:通过上述实验,对老年人群的ALT、AST、TP、ALB、Bun、Crea、UA等项目的参考区间进行初步分析,对现行参考区间的设定提供建议与参考。各实验室应建立自己的参考值范围。     

98.55W/cm2脉冲微波辐照对大鼠血清生化指标影响的研究

二级雄性Wistar大鼠35只,随机分为7组,每组5只,1组为空白对照,其余6组为辐照组以98.55W/cm^2的脉冲微波辐照动物,辐照后1、6、24h和7、14、28d分别采血,用Coulter-JTIR全自动生化仪进行测定,所有数据经Spss8.0统计软件处理,辐射后1h,CRE明显增高（P＜0.01）,而TP、ALB、AST、LD1、CK、ALP及CHOL均明显降低（P＜0.05）;辐射后6h,春CRE明显增高（P＜0.05）,而TP、ALB、LD1、ALP及CHOL均明显降低（P＜0.05）;辐照后24h,其ALT、GLU、BUN、CRE、AST、LD和HBDH明显升高（P＜0.05）,而LB和CHOL则明显降低（P＜0.05）;辐照后7d,其ALT、ALB、GLU、CRE、CK－MB和GGT明显升高（P＜0.05）,而P和CHOL则明显降低（P＜0.05);辐照后14d,其CRE、LD、CK－MB和GGT明显升高（P＜0.05）,而P和CHOL则明显降低（P＜0.01）;辐照后28d,其ALT、TP、BUN和CRE明显升高（P＜0.05）,而ALB、AST、LD1、CK、ALP和CHOL则明显降低（P＜0.01）。提示HPPMW可引起大鼠血清多种酶、蛋白及代谢产物的紊乱,即有早期影响,也表现为一定的持续效应。HPPMW可能引起大鼠多系统、多脏器的损伤。     

西安市某医院成人与儿童血清25羟基维生素D水平的研究

目的:比较西安市某医院不同年龄段的成人与儿童血清25羟基维生素D(25-OH-VD)水平,并探讨其水平与年龄之间的关系。方法:收集2017年1月-2018年1月西安高新医院检测血清25-OH-VD的2116例样本,比较成人与儿童血清中25-OH-VD水平的差异,分析成人与儿童血清中25-OH-VD水平与年龄之间的关系。结果:儿童组健康人群、低水平人群血清25-OH-VD水平均高于成人组(P0.05);儿童组健康人群、低水平人群血清25-OH-VD水平与年龄呈负相关(P0.05),成人组健康人群血清25-OH-VD水平与年龄呈正相关(P0.05)。结论:西安市某医院的儿童血清25-OH-VD水平高于成人,且成人与儿童的血清25-OH-VD水平与年龄存在一定的关系,有必要建立不同的参考区间,为临床诊治提供更精确的依据。     

引入西藏高原家兔的血清生化指标测定

目的了解和掌握引入西藏高原家兔的生理生化指标,以便为实践教学、临床诊疗和科学研究提供参数。方法采用朗道全自动生化分析仪对引入西藏高原20年的加利福尼亚兔(Californian rabbit)和中国白兔(Chinese white rabbit)的12项血清生化指标进行了测定。结果加利福尼亚兔在品种内比较发现TP、ALB、A/G、CRE、CHOL、LDH指标表现极强的雄兔特征(P0.01),而ALT、GLU测定值雌兔明显高于雄兔(P0.05);中国白兔在品种内比较发现CRE、LDH指标显示差异有显著性(P0.01),CRE指标是雄兔高于雌兔,LDH参数是雌兔高于雄兔。加利福尼亚雄兔与中国白雄兔的参数比较中发现AST、TP、ALB、GLO、A/G、GLU、CRE、LDH指标显示差异有显著性(P0.01),而TG、BUN指标显示差异有显著性(P0.05);加尼福利亚母兔与中国白母兔的参数比较中发现ALT、GLO、CRE、TG指标显示差异有显著性(P0.05),AST、TP、BUN、LDH指标显示差异有显著性(P0.01)。结论实验结果将为西藏高原的教学、临床诊疗和科学研究提供生理数据参考。     

血清胱抑素C 与乙型肝炎性肝硬化肝功能损害程度的相关性分析

目的:探讨血清胱抑素C(Cys C)与乙型肝炎性肝硬化患者肝功能损伤程度的相关性分析。方法:将98例乙型肝炎性肝硬化患者按照肝功能分级的Child-Pugh评分将分为A组(Child A级,n=45例)、B组(Child B级,n=33例)、C组(Child C级,n=20例),同期选取本院门诊正常健康体检者为对照组,检测所有受试者的血清Cys C水平,比较各种之间相关指标的差异。结果:A组、B组、C组、对照组丙氨酸氨基转移酶(ALT)、天门冬氨酸转移酶(AST)、血清白蛋白(ALB)、血清Cys C及血清Cys C阳性率均有统计学差异(P0.05),C组血清Cys C、血清Cys C阳性率高于对照组(P0.05)、A组、B组,而B组高于对照组、A组(P0.05);血清Cys C阳性组与阴性组中含有Child A级、Child B级、Child C级患者的构成比具有统计学意义(P0.05),Cys C阳性组中的Child C级患者比例高于阴性组(P0.05);血清Cys C与Child-Pugh评分呈正相关(P0.05);Child-Pugh评分、ALT、AST与患者血清Cys C阳性呈正相关(P0.05);而ALB则与其呈负相关(P0.05);结论:乙型肝炎性肝硬化患者血清Cys C水平与肝功能损伤程度呈一定相关性,早期检测血清Cys C水平对于评估乙型肝炎性肝硬化患者的肝功能损害具有一定的临床意义。     

北京市老年人肝肾功能参数的参考值范围调查

目的：观察北京市60岁以上不同年龄段健康老年人肝肾功能检验项目水平的差异,建立各自的参考值范围。方法：随机挑选600例60岁以上的北京市健康老人,按照不同年龄段分为三组,空腹采血,采用Modular仪器及原装试剂测定血清ALT（丙氨酸氨基转移酶）、AST（天冬氨酸氨基转移酶）、TP（总蛋白）、ALB（白蛋白）、Bun（尿素氮）、Crea（肌酐）、UA（尿酸）水平,结果利用SPSS11.0进行统计分析,根据统计学结果,判断参考值范围,并比较不同组间水平的差异。同时随机选择371例健康青年,与老年组进行这些项目水平的比较。结果：统计学结果显示,AST、TP、UA组间无显著差异;ALT、ALB随年龄增加而下降;Bun、Crea随年龄增加而升高。结论：通过上述实验,对老年人群的ALT、AST、TP、ALB、Bun、Crea、UA等项目的参考区间进行初步分析,对现行参考区间的设定提供建议与参考。各实验室应建立自己的参考值范围。     

肾病综合征患者血脂代谢紊乱与血胆红素和蛋白代谢指标的相关性分析

目的:研究肾病综合征(NS)患者血脂代谢紊乱与血胆红素和蛋白代谢指标的相关性。方法:纳入我院从2017年5月～2019年5月收治的NS患者50例进行研究,记作病变组,另取同期于我院进行体检的健康人员50例作为健康组。检测两组血脂代谢相关指标[甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)]、血胆红素相关指标[总胆红素(TBIL)、直接胆红素(DBIL)以及间接胆红素(IBIL)]以及蛋白代谢相关指标[血清总蛋白(TP)、血清清蛋白(ALB)、尿微量清蛋白(UALB)]水平并进行对比,予以Pearson相关性分析各指标之间的相关性。结果:病变组TG、TC、LDL-C、ApoA1、ApoB水平均显著高于健康组,而HDL-C水平显著低于健康组(均P0.05)。病变组TBIL、DBIL、IBIL水平均显著低于健康组(均P0.05)。病变组TP、ALB水平均显著低于健康组,而UALB水平显著高于健康组(均P0.05)。经Pearson相关性分析可得:NS患者TG、TC、LDL-C、ApoA1、ApoB与TBIL、DBIL、IBIL、TP、ALB均呈负相关(均P0.05);TG、TC、LDL-C、ApoA1、ApoB与UALB均呈正相关(均P0.05);HDL-C与TBIL、DBIL、IBIL、TP、ALB均呈正相关(均P0.05);HDL-C与UALB呈负相关(均P0.05)。结论:NS患者血脂代谢紊乱和血胆红素、蛋白代谢指标密切相关,血胆红素、蛋白代谢指标可能参与了NS患者血脂代谢紊乱的机制。     

C57BL／6J-HBV转基因小鼠血清生化指标与性别年龄的关系

目的观察C57BL/6J-HBV乙型肝炎病毒转基因小鼠血清总胆红素（T-BIL）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总蛋白（TP）和白蛋白（ALB）与性别和年龄的关系,以及与遗传背景相同的C57BL／6J小鼠的差异。方法选取8周龄和24周龄的C57BL／6J-HBV转基因小鼠及C57BL／6J小鼠的血清测定T-BIL、ALT、AST、TP和ALB值。结果C57BL／6J-HBV转基因小鼠与同周龄同性别的C57BL／6J小鼠相比,T-BIL、ALT、AST、TP和ALB均存在显著差异（P〈0．05）;24周龄的C57BL／6J-HBV转基因小鼠ALT和AST与其8周龄鼠相比均存在显著差异（P〈0．05）。结论C57BL／6J-HBV转基因小鼠T-BIL、ALT、AST、TP和ALB值显著高于C57BL／6J小鼠;且C57BL／6J-HBV转基因小鼠ALT和AST值与年龄有关,与性别无关。     

Variation of plasma protein parameters in four free-ranging reindeer herds and in captive reindeer under defined feeding conditions

Plasma total protein (TP), albumin (ALB) and globulin (GLOB) concentrations and albumin/globulin ratio (A/G) were analysed from blood samples collected from free-ranging reindeer (Rangifer tarandus tarandus) herds at varying times of year. The same parameters were followed in nine captive reindeer with varying protein and energy intake. Variation in the blood constituents of free-ranging reindeer was analysed in relation to different extrinsic and intrinsic factors and compared to findings from captive animals, allowing the analysis of effects of protein and energy intake. There was large overall variation in TP, ALB, GLOB and A/G ratio in the free-ranging animals, ranging between 36-110 g/L, 18-59 g/L, 17-59 g/L and 0.5-2.1. The variation between months and years was significant for all variables except the A/G ratio, where no year effect was noted. Increase in live body mass was associated with a small significant increase and pregnancy with a small significant decrease in all dependent variables, except for the A/G ratio. Age did not have a significant effect on any of the blood constituents when body mass was included in the same model. In captive animals, feeding lichens with low protein content was related to a significant decline in TP, ALB and GLOB, but not in the A/G ratio, whereas feeding commercial ration increased plasma TP, ALB and GLOB significantly. Extrinsic factors such as season and year explained majority of variation in the blood constituents of free-ranging reindeer, whereas body mass, pregnancy and age had only a minor influence. It is concluded that plasma TP and ALB, and to a lesser extent GLOB and A/G ratio may serve as nutritional biomarkers of reindeer.     

Discriminant analysis of hematological and serum biochemical values in cynomolgus monkey (Macaca fascicularis) bred and reared under the indoor individually-caged conditions

The data on hematological and serum biochemical properties of laboratory-bred cynomolgus monkeys (Macaca fascicularis) at different ages were analyzed by discriminant analysis. All the animals had been bred and reared under uniform environmental conditions at Tsukuba Primate Center for Medical Science, N.I.H., Japan. The items used were as follows: red blood cell count (RBC), hematocrit value (Ht), hemoglobin concentration (Hb), mean corpuscular volume (MCV), white blood cell count (WBC), glutamic oxaloacetic transaminase activity (GOT), glutamic pyruvic transaminase activity (GPT), total protein concentration (TP), albumin concentration (ALB), albumin-globulin ratio (A/G), blood urea nitrogen (BUN), glucose concentration (GLU), total cholesterol concentration (TCHO), free cholesterol concentration (FCHO), triglyceride concentration (TG) and alkaline phosphatase activity (ALP). In total, 1086 animals in 10 age groups were examined. Data analyses were done with respect to the difference of sex. Discrimination was possible by Mahalanobis' generalized distance between centroids of groups. In canonical discriminant analysis (discriminant analysis with reduction of dimensionality), age was highly correlated to the value of the first canonical variate. From the approximate relative value of the eigenvector of the first canonical variate, the most discriminant variables are WBC, TP, ALB, A/G, TCHO, FCHO, TG, and ALP. It can be concluded that periodic measurement of these 8 parameters is necessary and sufficient to monitor the physiological conditions of growing monkeys.     

Multivariate analysis of hematological and serum biochemical values in female cynomolgus monkeys (Macaca fascicularis) aged less than a year

Hematological and serum biochemical data obtained from 91 laboratory-bred cynomolgus monkeys (Macaca fascicularis) aged 11 to 362 days were analyzed by the discriminant analysis. All animals used had been bred and reared under uniform environmental conditions at Tsukuba Primate Center for Medical Science, N. I. H., Japan. The examination items were as follows: red blood cell count (RBC), hematocrit value (Ht), hemoglobin concentration (Hb), mean corpuscular volume (MCV), white blood cell count (WBC), glutamic oxaloacetic transaminase activity (GOT), glutamic pyruvic transaminase activity (GPT), total protein concentration (TP), albumin concentration (ALB), albumin-globulin ratio (A/G), blood urea nitrogen (BUN), glucose concentration (GLU), total cholesterol concentration (TCHO), free cholesterol concentration (FCHO), triglyceride concentration (TG), alkaline phosphatase activity (ALP) and calcium concentration (Ca). The animals were divided into four groups (A: Suckling infants, less than 176 days old. B, C, D: Weanlings and juveniles, 121 to 220 days old, 221 to 280 days old, and 281 to 362 days old, respectively). Discrimination was possible among these four groups on the basis of the Mahalanobis' generalized distance. Regarding the canonical discriminant analysis (discriminant analysis with reduction of dimensionality), discrimination was possible. The suckling infant group could be discriminated from the juvenile groups by the first canonical variate. Concerning the juvenile groups, age was highly correlated to the value of the second canonical variate.2+ Judging from an approximate relative eigenvector value for the second canonical variate, the effective discriminant variables were WBC, TP, ALB, A/G, FCHO, TG, and ALP. It can be concluded that these eight parameters are important and useful for monitoring the physiologicals conditions of growing juvenile monkeys.     

Discriminant analyses for pregnancy-related changes in hematological and serum biochemical values in cynomolgus monkeys (Macaca fascicularis)

Hematological and serum biochemical data obtained from 45 non-pregnant and 142 pregnant cynomolgus monkeys (Macaca fascicularis) were analyzed by discriminant analyses. All animals used had been reared under uniform environmental conditions at Tsukuba Primate Center for Medical Science, N. I. H., Japan. The examination items were as follows: red blood cell count(RBC), hematocrit value(Ht), hemoglobin concentration(Hb), mean corpuscular volume(MCV), white blood cell count(WBC), total protein (TP), albumin (ALB), glucose (GLU), total cholesterol (TCHO), free cholesterol (FCHO), triglyceride (TG), and albumin-globulin ratio (A/G). The pregnant animals were divided into four groups according to their gestation ages (Group I: 1-40 days; II: 41-80 days; III: 81-120 days; and IV: more than 121 days). During pregnancy period (Groups II, III and IV), significant decrease of TP, ALB, TCHO, FCHO and TG was observed. RBC, Ht and Hb decreased in the last of the four periods of pregnancy (Group IV). Discrimination was possible between each of these four pregnant groups and the non-pregnant group on the basis of the Mahalanobis' generalized distance. To clarify the relationship among groups, canonical discriminant analysis (discriminant analysis with reduction of dimensionality) was carried out. As a result, the non-pregnant group and the pregnant I group could be clearly discriminated from the other pregnant groups (II, III, and IV) by the first canonical variate. Judging from the relative eigenvector value of each variable for the first canonical variate, the effective discriminant variables were RBC, Ht, Hb, MCV, TP and A/G.(ABSTRACT TRUNCATED AT 250 WORDS)     

埋植雌二醇对养殖中华鲟雌性幼鱼血清生理指标的影响

用硅橡胶作载体,将剂量30 mg/kg的雌二醇(estradiol)埋植入人工养殖11龄、平均体重46.00 kg的雌性中华鲟(Acipenser sinensis)的背部肌肉内。埋植前及埋植后的第30、120、200天从尾部取血,测定血清中总蛋白、白蛋白、球蛋白、甘油三酯、总胆固醇、血糖和卵黄蛋白原的含量。结果显示,与对照组相比,除血糖含量无显著变化外,实验组鱼血清中总蛋白、白蛋白、球蛋白、甘油三酯、总胆固醇和卵黄蛋白原的含量显著升高。此结果为进一步探索外源物质促进中华鲟卵巢发育的可能途径奠定前期基础。     

Canonical discriminant analysis for pregnancy-related changes in hematological and serum biochemical values in tamarins (Saguinus spp

Hematological and biochemical values obtained from 9 monkeys (Saguinus labiatus and S. mistax) during pre- and postpartum periods were analyzed by canonical discriminant analysis (discriminant analysis with reduction of dimensionality). All animals used were of wild origin and had been maintained under uniform environmental conditions at N. I. H., Japan. The items examined were as follows: white blood cell count (WBC), red blood cell count (RBC), hematocrit value (Ht), hemoglobin concentration (Hb), total protein concentration (TP), blood urea nitrogen (BUN), albumin concentration (ALB), albumin-globulin ratio (A/G), glutamic oxaloacetic transaminase activity (GGT), glutamic pyruvic transaminase activity (GPT), alkaline phosphatase activity (ALP) and total cholesterol concentration (CHO). The data obtained in the pre- and postpartum periods were divided into six chronological groups. The prepartum period was divided into Group I: weeks 15-10; Group II: weeks 9-7; Group III: weeks 6-4; and Group IV: weeks 3-0. The postpartum period was divided into Group V: weeks 0-4 and Group VI: weeks 5-7). In the later pregnancy period (Groups III and IV), significant decreases in RBC, Ht, Hb, TP and ALB, and a significant increase in CHO were observed. These values in the blood and serum continued after delivery (Groups V and VI). Results of canonical discriminant analysis showed that the value of the first canonical variate decreased according to the progress of pregnancy. The postpartum groups showed negative values. Although groups in the early