Chromosomal assignments of 23 biochemical loci of the rat by using rat x mouse somatic cell hybrids

A panel of 18 rat x mouse somatic cell hybrid clones segregating individual rat chromosomes in different combinations was used to assign 23 biochemical loci to rat chromosomes. The chromosomal locations for these 23 loci were determined as follows: GOT1 on rat chromosome 1; HAGH on 2; ACP2, ADA, GANC, ITPA, and SORD on 3; LDHB on 4; PEPB on 7; GLB1 and HEXA on 8; IDH1 on 9; UMPH2 on 10; GUSB on 12; FH and PEPC on 13; PEPS on 14; ESD and NP on 15; DIA4 on 19; and PP on 20. In addition, ACP1 and GLO1 were reassigned to rat chromosomes 6 and 20, respectively. The chromosomal assignments of these loci extends the known syntenic homologies among rats, mice, and humans.     

Feeding, oviposition and survival of Liriomyza trifolii (Diptera: Agromyzidae) on Bt and non-Bt cottons

The effects of Bt transgenic cottons (Bt-I expressing cry1Ac and Bt-II expressing cry1Ab and cry2Ab or cry1Ab and cry1Fa) and non-Bt cottons on feeding, oviposition and longevity of adults, and development and survival of Liriomyza trifolii larvae were studied under laboratory conditions; and infestation on four Bt and two non-Bt cotton traits were investigated under field conditions. Laboratory choice and no-choice tests showed that L. trifolii adults were capable of distinguishing between Bt cottons and non-Bt cottons. In a choice test on younger plants (4-5 leaves), the adults were found more often and made more feeding punctures (FP) on non-Bt cottons than on Bt cottons. On older plants (8-9 leaves), adults made the most FP on non-Bt cotton followed by those on Bt-II cottons and the least on Bt-I cotton. The females oviposited more eggs (6.7 eggs per leaf) on non-Bt cotton than on Bt-I (1.7 eggs per leaf) and Bt-II (0.8 eggs per leaf) cottons on younger plants and oviposited similar numbers of eggs (0.7-1.3 eggs per leaf) on non-Bt and Bt cottons on older plants. In a no-choice test, the females also fed more FP on non-Bt cottons than on Bt cottons on both younger and older plants. The females oviposited more eggs (15.6 eggs per leaf) on non-Bt cotton than on Bt-I (8.2 eggs per leaf) and Bt-II (6.5 eggs per leaf) cottons on younger plants and similar numbers of eggs (2.5-3.3 eggs per leaf) on non-Bt and Bt cottons on older plants. Larval and puparial survivals were not different among Bt and non-Bt cottons. The occurrence and damage of leafminers on cottons in the field showed that L. trifolii infested more plants and leaves and had more mines on non-Bt cotton than on Bt cottons.     

Nymph and adult biology of Dichelops melacanthus (Dallas) (Heteroptera: Pentatomidae) feeding on cultivated and non-cultivated host plants

The biology of the pentatomid Dichelops melacanthus (Dallas) feeding on cultivated and non-cultivated plants was studied in the laboratory. Nymph mortality varied from approximately 60 on corn (seed mature) to 77% on wheat (ear immature); no nymphs survived on seedlings of corn or wheat. Nymph developmental time on soybean, corn or wheat (seed, pod or ear) varied from 25.5 to 32.8 days. Body weight at adult emergence was similar and greater on most foods than on wheat ear. Nymphs fed preferentially on soybean (pod immature). On non-cultivated hosts, nymphs showed high mortality (73%) on crotalaria (pod immature); on tropical spiderwort (stem) all nymphs died. Nymphs took longer time to develop on crotalaria and/or on spiderwort than on soybean. Body weight at adult emergence did not differ on crotalaria or soybean. Survivorship decreased with time on most foods, with approximately 50% of adults alive at day 30. On corn and wheat seedlings approximately 80% of adults were dead on day 20. Adult longevity ranged 31-43 days, except on corn and wheat seedlings < 15 days. Females % ovipositing peaked ( approximately 76%) on soybean (pod or seed immature), and was minimum ( approximately 9%) on wheat ear (immature); no females reproduced on seedlings of corn or wheat. Preoviposition period was shorter ( approximately 12 days) on soybean (immature pod or seed) and longer ( approximately 37 days) on wheat (ear immature). Fecundity was similar and higher on all foods than on wheat (ear immature). Body weigh gain occurred on all foods, but on corn and wheat seedlings. Adults fed preferably on soybean (pod immature and seed mature); wheat (seedling) was the least preferred food.     

不同寄主植物对云南紫胶虫自然种群的影响

研究了云南紫胶虫(Kerria yunnanensis)在聚果榕、南岭黄檀、钝叶黄檀和偏叶榕上的发育、存活和繁殖情况.结果表明：在自然条件下,云南紫胶虫在钝叶黄檀上的种群初始密度最高,为181.17头·cm^-2,聚果榕上最低,为145.27头·cm^-2;雄虫占的比例以在南岭黄檀上最少,为0.19,偏叶榕和聚果榕上最多,为0.24;从幼虫到成虫的累积死亡率,南岭黄檀上最高,为90.03%,偏叶榕上最低,为87.05%;完成世代时间以在南岭黄檀上最长,为156 d,聚果榕上最短,为140 d;平均怀卵量以在南岭黄檀上最高,为536粒,聚果榕上最低,为345.86粒;虫体质量以在南岭黄檀上最大,为12.62 mg,偏叶榕上最小,为9.03 mg;世代净增值率以在南岭黄檀上最高,为48.51,聚果榕上最小,为32.79.综合比较4种不同寄主植物,南岭黄檀是云南紫胶虫夏季世代种群生长发育和繁殖最适宜的寄主.     

Patterns of species diversity on an oceanic versus a continental island mountain: a hypothesis on species diversification1

Abstract. The vegetation on a wet mountain slope on Haleakala (an oceanic island) is compared with that on Kinabalu (a continental island) to examine relationships between regional floristic richness and α- and β-diversities. The two mountains are similar in their constant tropical climate, generic and family-level floristic elements and geological age of the summit regions, but different in regional floristic richness (rich on Kinabalu vs. poor on Haleakala). α-diversity of canopy and subcanopy tree species was much higher on Kinabalu than in comparable zones on Haleakala. Average turnover rate of species (as logarithmic community similarity) on the slope was one order of magnitude greater on Kinabalu than on Haleakala (0.127 vs. 0.017 per 100 m alt.). While there were genera with wide altitudinal ranges on both mountains, a large proportion of the genera was differentiated into parapatric altitudinal congeners on Kinabalu. By contrast, most genera are altitudinally monotypic on Haleakala. The number of sympatric congeners per genus, and the frequency of multi-specific genera per plot were high on lower slopes but decreased with increasing altitude on Kinabalu, whereas the values were low across all altitudes on Haleakala. These patterns suggest that sympatric and parapatric species radiation was less on Haleakala than on Kinabalu. This may be related to Haleakala's initially poor and disharmonic flora.     

酸沉降对森林生态系统影响的研究现状及展望

酸沉降影响下物质循环及其不平衡研究;酸沉降对土壤理化性质的影响;森林水化学方面的研究;酸沉降下重金属的活化研究;酸沉降对植物生长的影响研究;酸沉降和气候变化对森林的影响;模拟酸雨对土壤理化性质和植物生长的影响;酸沉降下土壤风化问题的研究;运用模型对酸化问题的研究;森林土壤人为和自然的酸化;酸沉降临界负荷的研究;酸沉降和其它污染物对植物的联合影响;酸化土壤恢复研究等方面介绍了酸沉降对森林生态系统影响的研究现状,并阐明了今后研究的方向及应该注意的问题。     

取食不同品种棉花的棉蚜对龟纹瓢虫功能反应的影响

本文以GK12、33B和SGK3213种转基因棉和对应的3种常规棉泗棉3号、33及石远321为材料,通过室内测定,研究了取食转基因棉的棉蚜却h／sgossypiiGlover对龟纹瓢虫Propylaea japonica Thunberg1—4龄幼虫功能反应的影响。结果指出,龟纹瓢虫对来自转基因棉田蚜虫的捕食量大多高于常规棉田,且捕食量随猎物密度的增加而增大。在1、2龄幼虫期,龟纹瓢虫取食转双价基因棉田蚜虫的数量多高于取食转单价基因棉田的蚜虫数量,但是在3、4龄幼虫时,龟纹瓢虫取食转双价棉田蚜虫的数量多低于取食转单价棉田的蚜虫数量。龟纹瓢虫对来自转基因棉田棉蚜的捕食功能反应符合HollingII型。龟纹瓢虫1龄幼虫取食GK12和SGK3212个转基因棉田蚜虫的最大日捕食量大于对照,处理时间短于对照;龟纹瓢虫P．japonica2、3龄幼虫取食33B和SGK3212个转基因棉田蚜虫A．gossypii的日最大捕食量大于对照,处理时间短于对照;4龄时取食转基因棉田蚜虫的数量都大于对照,处理时间均短于对照,其中取食转双价基因棉SGK321棉田蚜虫的日最大捕食量小于取食单伊棉田蚜虫的日最大捕食量。     

Effect of host-plant manipulation by a gall-inducing insect on abundance of herbivores on chestnut trees

We investigated plant-mediated effects of the stem gall wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), on other herbivores on the chestnut tree Castanea crenata. In the early season, leaves emerged earlier and in greater numbers on galled shoots than on ungalled shoots. On galled shoots the leaf to shoot biomass ratio was lower and the leaves were physically different. In May and June the concentration of nitrogen in leaves was higher on galled shoots than on ungalled shoots. In July, the water content of leaves was lower on galled shoots. In May and June, the number of aphids, Myzocallis kuricola Matsumura (Homoptera: Aphidoidea), on leaves was higher on galled shoots than on ungalled shoots, but the opposite was true at the end of July. Laboratory experiments showed that aphid fecundity and body weight decrease were higher in May and June when aphids fed on leaves on a galled shoot than when they fed on those on ungalled shoots. In contrast, aphid performance in July was greater on ungalled leaves than on galled leaves. Our findings suggest that gall initiation in a chestnut tree affected aphid performance by affecting host plant quality.     

Continuous long-term somatic embryogenesis in alfalfa

Summary Somatic embryogenic callus was induced on two induction media, B5h and SH4K. Embryos formed on the callus induced on B5h medium when the callus was still on the induction medium. On the other hand, embryos could not form on the callus induced on SH4K medium unless the callus was transferred to a growth regulator-free medium. Callus induced and maintained on B5h medium lost embryogenic capability quickly during the subculture. Callus induced and maintained on SH4K medium, however, consistently remained highly embryogenic. The callus mass showed steady increase during its maintenance on SH4K medium. The embryos induced on SH4K medium showed vigorous germination. Normal and fully fertile plants were recovered from the embryos developed from the callus maintained on SH4K medium.     

8种致病真菌在5种材料上的存活时间研究

目的了解实验所用常见致病真菌能否在常用医用原材料上生存及生存时间。方法选择8种常见致病真菌,活化后将它们分别接种在5种常用的医用原材料上,接种后及以后的每24h将接种有真菌的医用材料放入巯基乙酸盐液体培养基中并观察能否生存,记录存活时间。结果红色毛癣菌和须癣毛癣菌在棉布、纸板、铁皮、玻璃表面存活25～28d,在地板胶表面存活14～15d;新生隐球菌在棉布、纸板、铁皮表面存活24～27d,在玻璃和地板胶表面存活14～18d;茄病镰刀菌在上述5种材料上存活22～27d;石膏样小孢子菌在棉布、纸板、铁皮和玻璃表面存活20～27d,在地板胶表面存活8d;申克孢子丝菌在棉布、纸板、玻璃和地板胶表面存活17～24d,在铁皮表面存活10d;白念珠菌在棉布、纸板、铁皮和地板胶表面存活15～20d,在玻璃表面存活4d;犬小孢子菌在棉布、纸板、铁皮、玻璃和地板胶上存活时间分别为10d、9d、3d、2d和1d。结论实验所用各种真菌在医用原材料上都能存活,其时间长短不仅与菌种有关,还与所存在的材料有关。真菌在吸水性强、表面粗糙的材料(棉布、纸板)上的平均存活时间长于在吸水性差、表面光滑的材料(玻璃、铁皮和地板胶)。     

中国春孢锈菌属增补

本文对我国春孢锈菌属(形式属)增补了以下9个种：1．腺梗菜春孢锈菌Aecidium adenocauli Syd． 寄主：腺梗菜Adenocaulon himalaicum Edgew (菊科Compositae) 2．臭椿春孢锈菌(新种) Aecidium ailanthi J．Y．Zhuang sp．nov．寄主：臭椿 Ailanthus altissima Swingle (苦木科Simaroubaceae)3.筋骨草春孢锈菌 Aecidium alugae Syd．寄主：紫背金盘 Ajuga nioponensis Makino (唇形科 Labiatae) 4．八角枫春孢锈菌 Aecidium alangii Hirats．＆ Yosh 寄主：长毛八角枫 Alangium kurzii Craib,瓜木A platanifoHum Harms (八角枫科 Alangiaeeae) 5．紫珠生春孢锈菌(新种) Aecidium callicarpivola J Y．Zhuang sp．nov.寄主：杜虹花 Callicarpa oedunculata R,Br．(马鞭草科Verbenaeeae) 6．吴茱萸春孢锈菌(新种) Aecidium evodiae J．Y．Zhuang sp．nov． 寄主：吴茱萸 Evodia rutaecarva (Juss．)Benth．(芸香科Rutaceae) 7．杨叶木姜子春孢锈菌(新种) Aecidium litseae-populifoliae J．Y．Zhuang sp．Nov．寄主：杨叶木姜子 Litsea povulifolia Gamble (樟科Lauraceae) 8．青海春孢锈菌(新种) Aecidium qinghaiense J Y．Zhuang sp．nov． 寄主：莴苣属一种Lactuca sp．(菊科Compo sitae) 9．合掌消春孢锈菌 Aecidium vincetoxici P．Henn．＆ Shirai 寄主：牛皮消 Cynanchum auriculatum Royle (萝藦科 Asclepiadaceae)     

异迟眼蕈蚊在不同植物上的生长发育及种群参数

【目的】异迟眼蕈蚊Bradysia difformis Frey的幼虫取食为害作物的地下部分,影响作物的品质,为了明确韭菜、蚕豆、生菜、白菜和甘蓝5种植物对异迟眼蕈蚊生长发育以及繁殖的影响。【方法】本试验采用室内人工饲养测定的方法,研究了5种不同植物对异迟眼蕈蚊生长发育,繁殖力和存活率的影响,并统计了其对异迟眼蕈蚊种群参数的影响。【结果】结果表明:卵到蛹的发育历期依次为甘蓝、白菜、韭菜、生菜、蚕豆;5种植物对雌雄虫寿命影响不显著,对雌虫产卵量以及蛹重均有影响,其中在韭菜上的产卵量最大,甘蓝最少,在韭菜上蛹最重,生菜上蛹最轻;异迟眼蕈蚊的存活率随着生长发育降低,总体在韭菜上的存活率高于其他寄主植物,在生菜上的存活率均最低。统计分析不同植物对异迟眼蕈蚊种群参数的影响,净增殖率和内禀增长率在韭菜上最大而在甘蓝上最小;平均世代周期在蚕豆上最短,甘蓝上最长;种群加倍时间在韭菜上最短,而在甘蓝上最长。【结论】由此可知,异迟眼蕈蚊均可以在韭菜,蚕豆,生菜,白菜和甘蓝上完成生长发育及繁殖,其对5种植物的适应性依次为:韭菜、蚕豆、白菜、甘蓝和生菜。     

大白菜相对于结球甘蓝连锁群特异SSR标记的筛选

以9个大白菜品种和10个结球甘蓝品种为试材,对位于芸薹属A基因组10个连锁群的207对SSR引物进行PCR筛选,共筛选出33个大白菜相对于结球甘蓝的特异SSR标记,涉及了大白菜的10个连锁群,其中位于A1连锁群3对,A2连锁群4对,A3连锁群5对,A4连锁群2对,A5连锁群3对,A6连锁群4对,A7连锁群2对,A8连锁群1对,A9连锁群2对,A10连锁群7对。为进一步利用SSR标记鉴定结球甘蓝—大白菜异附加系奠定了基础。     

Antibody-induced redistribution of normal and tumor associated surface antigens

Redistribution (capping) of normal and tumor-associated surface antigens was studied on murine and human cells by the indirect membrane immunofluorescence (MIF) technique. The capping of H-2 isoantigens was compared on normal mouse T-lymphocytes and on YAC cells, a Moloney leukemia virus (MLV) induced lymphoma. H-2 and Moloney virus induced cell surface antigen (MCSA) capping was compared on three YAC lines with different MCSA concentrations. H-2 and tumor-associated surface antigen capping was compared on two polyoma induced sarcoma lines and five methylcholanthrene induced sarcoma lines. In the human system, IgM-capping was compared on normal lymphocytes and on the Burkitt lymphoma derived Daudi line. Capping of HL-A and the Epstein-Barr virus (EBV) determined membrane antigen (MA) was compared on the Burkitt lymphoma derived line Maku and on EBV-superinfected Daudi cells. H-2 antigens on normal murine cells capped more promptly and on a larger fraction of the cell population on the various tumor cells. Surface associated IgM showed a better capping on normal lymphocytes than on Daudi cells. All tumor associated antigens except MCSA, showed good capping. MCSA was almost completely refractory to capping. Increasing concentrations of MCSA appeared to inhibit the capping of H-2 on the YAC sublines with different concentrations of MCSA. The polyoma induced ascites sarcoma (SEWA) did not cap either with regard to H-2 or the polyoma determined surface antigen.     

Computer Programme to Estimate Recurrence Risks for Multifactorial Familial Disease

A computer programme to estimate recurrence risks for multifactorial genetic disease in affected families uses parameters on population prevalence and on heritability of liability of the condition and details of the family history. Information on affected and unaffected relatives (first-degree, second-degree, and third-degree) and on sex or age effects on prevalence and on heritability can all be accommodated by the programme.     

Two new species of Leporinus (Characiformes: Anostomidae) from the Brazilian Amazon,and redescription of Leporinus striatus Kner 1858

Two new species of Leporinus are described from tributaries of the Rio Amazonas in Brazil. One species is known from the Jari and Tapajós River basins, and is identified on the basis of a gas bladder reduced in size, a dark midlateral stripe on the body, dark transverse bars on the dorsum, a subinferior mouth, three teeth on the premaxilla, four teeth on the dentary and 16 scale rows around the caudal peduncle. The second new species is known from the Tocantins, Xingu and Tapajós River basins, and is identified on the basis of three dark longitudinal stripes on the body, a subinferior mouth, three teeth on the premaxilla, four teeth on the dentary and 12 scale rows around the caudal peduncle. In addition, Leporinus striatus is redescribed based on type and additional specimens from the Río de La Plata, Amazonas, Orinoco, Atrato, Magdalena and Sinu River basins. Leporinus striatus is identified on the basis of four dark longitudinal stripes on the body, a subterminal mouth, three teeth on the premaxilla, four teeth on the dentary and 16 scale rows around the caudal peduncle.     

The effects of improvement of upland, marginal grasslands on the breeding success of Lapwings Vanellus vanellus and other waders

Data on the breeding success of Lapwings Vanellus vanellus on unimproved and improved grassland, with comparative data for arable land, were obtained from a study on 760 Lapwing clutches. The study was undertaken in the Eden Valley, Cumbria, and Teesdale, Co. Durham, between 1985 and 1987. First clutches were larger on unimproved grassland with a mean of 3.73 eggs compared to 3.61 eggs on improved grassland. In contrast, replacement clutches were larger on improved areas (3.90 eggs) than on unimproved (3.47 eggs). On average, 40% of eggs laid on unimproved pastures hatched compared to only 17% on improved pastures. No significant difference in hatching success was found between unimproved and improved meadows with 32% and 22% of eggs hatching, respectively. Overall, 73% of unsuccessful first clutches were replaced on unimproved pastures, whereas on meadows and improved pastures combined, only 32% were replaced. Survival of small chicks was highest on unimproved areas. Production on unimproved areas was sufficient to replace adult losses and so maintain numbers, whereas on improved land production was too low to maintain existing breeding densities. Hatching success on unimproved areas was similar for the four species of wader considered. On improved areas, Redshank hatching success was relatively high, with 54% of pairs producing chicks, compared to 35% for Lapwing and 23% for Curlew.     

红树林湿地恢复技术的研究进展

本文从红树林育苗造林技术、次生林改造技术和红树植物引种试种与种源选择的研究等方面试述红树林湿地恢复技术的研究进展,并针对我国的目前现状,提出今后亟待加强研究的如下几个方面:加强红树植物引种扩种,恢复植被,提高红树林生态工程质量的原理研究;监测外来引进种对我国红树林湿地的生态影响;研究半红树植物在沿海防护林体系工程建设中的作用;加强红树植物对潮汐水位适应能力的研究;开展红树人工林生态恢复过程中的综合定位观测等。     

中国海南省锈菌资料补编

补充记载了采自海南省的9种锈菌。甲竹Bambusa remotiflora上的华南柄锈菌Puccinia austrosinensis、大花紫薇Lagerstroemia speciosa上的紫薇夏孢锈菌Uredo lagerstroemiae和海南薯Ipomoea sumatrana上的宽乳单胞锈菌Uromyces latimammatus为新种;厚叶素馨Jasminum pentaneurum上的巴氏查科锈菌Chaconia butleri、猪肚木Canthium horridum上的鱼骨木驼孢锈菌Hemileia canthii、牛筋果Harrisonia perforata上的牛筋果无眠多胞锈菌Kuehneola harrisoniae、毛咀签Gouania javanica上的椴叶咀签柄锈菌Puccinia gouaniae-tiliaefoliae、蒲桃Syzygium jambos上的番石榴柄锈菌Puccinia psidii以及象草Pennisetum purpureum上的钝叶草柄锈菌Puccinia stenotaphri为中国新记录。引证的标本均保存于中国科学院菌物标本馆（HMAS）。     

Potential adaptation of a Q biotype whitefly population from poinsettia to field crops

Abstract The invasive Q biotype whitefly was first detected in the US on poinsettia in 2004 and is still not a pest outside of greenhouse environments in the US. To assess the potential for the establishment of the Q biotype on field crops, population cage experiments were conducted to compare the performance of a poinsettia‐derived Q population named P’06 on poinsettia and six field crops (alfalfa, tomato, melon, cotton, cowpea and cabbage). P’06 adults reared on poinsettia as nymphs laid eggs on all six field crops. Significantly more eggs were laid on alfalfa, tomato, melon and cotton than on cabbage, cowpea and poinsettia. These eggs hatched and the nymphs developed to adults on the six field crops. Relative to poinsettia, whitefly survival was similar on cowpea, alfalfa, tomato and cabbage, but significantly higher on cotton and melon. Moreover, P’06 had significantly shorter development times from egg to adult on cotton, melon, cowpea, tomato and alfalfa than they did on poinsettia. However, the F1 adults raised on the six field crops had significantly shorter lifespans and laid 11‐ to 18‐fold fewer eggs than did the F1 adults raised on poinsettia. Taken together, while P’06 may have some potential to establish on field crops, the shorter lifespans and extremely low fecundities of the F1 adults raised on the six field crops suggests that P’06 is incapable of rapidly adapting to them. Poor adaptation to field crops may explain, at least partially, why the Q biotype has not established in the US field system.