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Goyal Pooja Devi Ritu Verma Bhawana Hussain Shahnawaz Arora Palak Tabassum Rubeena Gupta Suphla 《Protoplasma》2023,260(2):331-348
Protoplasma - The recent advancements in sequencing technologies and informatic tools promoted a paradigm shift to decipher the hidden biological mysteries and transformed the biological issues... 相似文献
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Physical and functional interactions between Werner syndrome helicase and mismatch-repair initiation factors 总被引:1,自引:0,他引:1
Saydam N Kanagaraj R Dietschy T Garcia PL Peña-Diaz J Shevelev I Stagljar I Janscak P 《Nucleic acids research》2007,35(17):5706-5716
Werner syndrome (WS) is a severe recessive disorder characterized by premature aging, cancer predisposition and genomic instability. The gene mutated in WS encodes a bi-functional enzyme called WRN that acts as a RecQ-type DNA helicase and a 3′-5′ exonuclease, but its exact role in DNA metabolism is poorly understood. Here we show that WRN physically interacts with the MSH2/MSH6 (MutSα), MSH2/MSH3 (MutSβ) and MLH1/PMS2 (MutLα) heterodimers that are involved in the initiation of mismatch repair (MMR) and the rejection of homeologous recombination. MutSα and MutSβ can strongly stimulate the helicase activity of WRN specifically on forked DNA structures with a 3′-single-stranded arm. The stimulatory effect of MutSα on WRN-mediated unwinding is enhanced by a G/T mismatch in the DNA duplex ahead of the fork. The MutLα protein known to bind to the MutS α–heteroduplex complexes has no effect on WRN-mediated DNA unwinding stimulated by MutSα, nor does it affect DNA unwinding by WRN alone. Our data are consistent with results of genetic experiments in yeast suggesting that MMR factors act in conjunction with a RecQ-type helicase to reject recombination between divergent sequences. 相似文献
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