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1.
It is shown that cryptand complexes containing copper inhibit sodium-calcium exchange in sarcolemma of cardiomyocytes. The complexes studied exhibit the superoxide dismutase activity. The binding of copper ions by cryptand prevents the development of cariotoxic effects of the former. Low toxicity, the combined superoxide activity and that inhibiting sodium-calcium exchange of cryptand complexes with copper (II) ions makes the further search for cardioprotective compounds perspective within the series of cryptand copper complexes. 相似文献
2.
E P Lukashev A A Kononenko N I Zakharova A B Rubin G N Novodarova 《Biokhimii?a (Moscow, Russia)》1980,45(2):273-284
The effects of cobalt and copper o-phenanthroline complexes on electron transfer and energy coupling activity in the reaction center and chromatophore preparations of purple bacteria were studied. In terms of their effects on the systems under study these complexes fall into two groups, i.e. cobalt complexes with a high electron transfer activity, which stimulate membrane energization, and copper complexes which contribute to the chromatophore membrane deenergization. Among a variety of complexes studied the perchlorate tris-o-phenanthroline complex Co(II) and the chloride 4,7-diphenyl-o-phenanthroline complex Cu(II) were found to have the highest activity. Both cobalt and copper o-phenanthroline complexes may be a promising tool for regulating bioenergetic processes. 相似文献
3.
Binuclear copper(II) complexes prepared with the binucleating ligand 2,6-diformyl-4-methyl-phenol were attached as pendant groups to the polymers polyethyleneimine and polyvinylaminevinyl sulfonate sodium salt. The polymer-copper(II) complexes were characterized from optical and magnetic studies and their catecholase activities were measured in water. Polyvinylamine-vinylsulfonate-copper(II) is insoluble in water and showed little if any catecholase activity. The polyethyleneimine-copper complexes were reasonably soluble in water and demonstrated good catecholase activity. The catecholase activity of the polyethyleneimine-copper complexes increased in activity with an increase in the ratio of polymer-to-copper content (based on a constant copper concentration). 相似文献
4.
Diego La Mendola Serena Caminati Salvatore S. Emmi Giuseppe Maccarrone Adriana Pietropaolo 《Journal of inorganic biochemistry》2009,103(2):195-204
Potentiometric and spectroscopic (UV-Vis, CD and EPR) studies were carried out on copper(II) complexes with chicken prion protein N-terminal fragments, Ac-(PHNPGY)4-NH2, and the mutated residue, Ac-(PHNPGF)4-NH2, to assess the role of tyrosine in the copper coordination. Both thermodynamic and spectroscopic results indicate that chicken prion fragments are not able to bind more than two copper ions and only with the involvement of side chain tyrosine groups. The prevailing complex shows one copper ion bound to four imidazole nitrogen atoms in the 1:1 metal to ligand ratio systems. The superoxide dismutase (SOD)-like activity of copper(II) complexes with the avian peptides and mammal analogue, Ac-(PHGGGWGQ)4-NH2, was also investigated by means of Pulse radiolysis. The copper(II) complexes with avian peptides do not display SOD-like activity, while very low activity has been detected for the copper(II) complexes with mammalian tetraoctarepeat. 相似文献
5.
The antitumor activity of forty nine different metal complexes of the first transition series against mouse leukemia L 1210 cells and of two of the complexes against Ehrlich ascites carcinoma have been tested in vitro by the method described in this paper. Eight complexes showed a 50% inhibition of tumor cell division at concentration level 5–6 μg/ml of the complex for the former and two most effective complexes also for the latter. The trans-bis-(salicylaldoximato)copper(II) and trans-bis(resorcylaldoximato)copper(II) complexes were found to possess the highest antitumor activity. 相似文献
6.
Zhen Zhang Caifeng Bi Sara M. Schmitt Yuhua Fan Lili Dong Jian Zuo Q. Ping Dou 《Journal of biological inorganic chemistry》2012,17(8):1257-1267
Indole-3-acetic acid and indole-3-propionic acid, two potent natural plant growth hormones, have attracted attention as promising prodrugs in cancer therapy. Copper is known to be a cofactor essential for tumor angiogenesis. We have previously reported that taurine, l-glutamine, and quinoline-2-carboxaldehyde Schiff base copper complexes inhibit cell proliferation and proteasome activity in human cancer cells. In the current study, we synthesized two types of copper complexes, dinuclear complexes and ternary complexes, to investigate whether a certain structure could easily carry copper into cancer cells and consequently inhibit tumor proteasome activity and induce apoptosis. We observed that ternary complexes binding with 1,10-phenanthroline are more potent proteasome inhibitors and apoptosis inducers than dinuclear complexes in PC-3 human prostate cancer cells. Furthermore, the ternary complexes potently inhibit proteasome activity before induction of apoptosis in MDA-MB-231 human breast cancer cells, but not in nontumorigenic MCF-10A cells. Our results suggest that copper complexes binding with 1,10-phenanthroline as the third ligand could serve as potent, selective proteasome inhibitors and apoptosis inducers in tumor cells, and that the ternary complexes may be good potential anticancer drugs. 相似文献
7.
Kowol CR Heffeter P Miklos W Gille L Trondl R Cappellacci L Berger W Keppler BK 《Journal of biological inorganic chemistry》2012,17(3):409-423
Intracellular generation of reactive oxygen species (ROS) via thiol-mediated reduction of copper(II) to copper(I) has been
assumed as the major mechanism underlying the anticancer activity of copper(II) complexes. The aim of this study was to compare
the anticancer potential of copper(II) complexes of Triapine (3-aminopyridine-2-carboxaldehyde thiosemicarbazone; currently
in phase II clinical trials) and its terminally dimethylated derivative with that of 2-formylpyridine thiosemicarbazone and
that of 2,2′-bipyridyl-6-carbothioamide. Experiments on generation of oxidative stress and the influence of biologically relevant
reductants (glutathione, ascorbic acid) on the anticancer activity of the copper complexes revealed that reductant-dependent
redox cycling occurred mainly outside the cells, leading to generation and dismutation of superoxide radicals resulting in
cytotoxic amounts of H2O2. However, without extracellular reductants only weak intracellular ROS generation was observed at IC50 levels, suggesting that cellular thiols are not involved in copper-complex-induced oxidative stress. Taken together, thiol-induced
intracellular ROS generation might contribute to the anticancer activity of copper thiosemicarbazone complexes but is not
the determining factor. 相似文献
8.
Raúl Saraiva Sandra Lopes Mariana Ferreira Florbela Novais Eulália Pereira Maria J. Feio Paula Gameiro 《Journal of inorganic biochemistry》2010,104(8):843-850
Solution behaviour of enrofloxacin complexes with copper(II), nickel(II), cobalt(II) and zinc(II) in the presence and absence of 1, 10-phenanthroline was studied in aqueous solution, by potentiometry. The results obtained show that under physiological conditions (micromolar concentration range and pH 7.4) only copper(II) forms stable complexes. Binary copper(II)/enrofloxacin and ternary copper(II)/enrofloxacin/phenanthroline complexes were synthesised and characterized by elemental analysis, UV–visible spectroscopy and FTIR. The antimicrobial activity of these complexes and of copper(II)/enrofloxacin and copper(II)/enrofloxacin/phenanthroline solutions, prepared by mixing of the individual components in the same stoichiometric proportion and concentration range used for the synthesised complexes, was tested against two different Escherichia coli strains. Although, at a glance, the results point to a possible use of both complexes as metalloantibiotics, a detailed analysis shows that, at biological concentrations, the copper(II) binary complex does not exist and the antimicrobial activity observed is a consequence of its dissociation into free enrofloxacin. Consequently, only the ternary complex seems worth pursuing as a possible antimicrobial agent candidate. Moreover, as the biological studies showed, both the synthesised complexes and the solutions prepared by mixing the components exhibited the same behaviour. Hence, a new, faster and accurate methodology to screen metalloantibiotics prior to synthesis of the complexes is proposed. 相似文献
9.
The antioxidant activity of dihydroquercetin (DHQ) complexes with zinc, copper(II) and calcium was studied in vitro in blood plasma of healthy donors. The state of lipid peroxidation (LPO) in blood plasma was assessed by the content of malondialdehyde (MDA), diene (DC) and triene (TC) conjugates. The effect of DHQ and the complexes on the activity of the catalase enzyme in blood plasma was determined. It was found that DHQ complex with zinc ion reduces the MDA content in blood plasma by 14.9% compared with the control, which is twice as high as for DHQ (7.5%). The corresponding parameters of DHQ complexes with copper(II) and calcium ions were 11.2 and 3.7%, respectively. The effect of the complexes on the decrease in the DC and TC content in blood plasma compared with the control is comparable with the corresponding parameters for DHQ. The DHQ complex with zinc ion increases the catalase activity by 1.5% compared with DHQ. The complexes containing copper(II) and calcium ions increase the catalase activity no more than DHQ. 相似文献
10.
Francesco Bellia Giuseppe Maccarrone Daniele Vitalini Salvatore Sortino Enrico Rizzarelli 《Inorganica chimica acta》2007,360(3):945-954
Copper(II) complexes of the β-cyclodextrin (β-CD) functionalized with homocarnosine (HC) in the primary (CDHC6) and secondary rim (CDHC3) were characterized by means of different spectroscopic techniques such as UV-Vis absorption, circular dichroism, electron paramagnetic resonance and electron-spray mass spectrometry. Taken together, all the spectroscopic parameters indicate the formation of different copper(II) complex species at various pH values. In the CDHC3 copper(II) complex species, a direct involvement of the secondary hydroxyl group 2 of functionalized β-CD’s ring has been pointed out.The antioxidant activity of the copper(II) complexes of the two derivatives was determined through pulse radiolysis measurements. The results obtained provide direct evidence for a high catalytic activity of both complexes towards the dismutation of the superoxide anion radical. It is also demonstrated that the complex formation is not detrimental to the excellent scavenger activity exhibited by the ligands alone towards hydroxyl radicals. These copper complexes then represent very intriguing antioxidant agents against well known toxic reactive oxygen species. 相似文献
11.
Several copper(II) complexes with aminoacids and peptides are known to show superoxide dismutase (SOD)-like activity. EPR spectroscopy has proved to be a useful tool for studying the complex equilibria of the copper(II) ion and various ligands of biological importance in solution. In the present work, a variety of copper(II) complexes with di-, tri- and tetra-peptides containing only glycine residues (GG, GGG and GGGG) and others containing a histidyl residue in different positions (HGG, GHG, GGH and GGHG) have been investigated. EPR parameters obtained by extensive use of computer simulation of spectra lead to reliable spin Hamiltonian EPR parameters at both room temperature and in frozen solution. The molecular orbital coefficients computed from the anisotropic EPR data and the d-d electronic energies are used to characterize different arrangements of the complexes. Estimation of the scavenger activity of the complexes due to the particular environment created by the ligands around copper is discussed in the frame of the structure-activity relationship. 相似文献
12.
Jn Van
o Oga vajlenov Eva Ra
ansk Jan Muselík Jindra Valentov 《Journal of trace elements in medicine and biology》2004,18(2):155-161
Considering the important role of antioxidants in biological systems, the group of copper(II) complexes derived from salicylaldehyde and alpha- or beta-alanine and its thiourea derivative and copper(II) complexes derived from pyruvic acid and beta-alanine were studied. The antiradical activity of the tested compounds was studied by both in vitro and in vivo methods. The chemical methods based on inhibition of INT-formazane or 3-nitrotyrosine formation were used for the evaluation of SOD-mimic and antiperoxynitrite activity, respectively. In the case of in vivo activity evaluation, an alloxan-induced diabetes mellitus model in mice was used, the mechanism of action of alloxan being closely connected with the formation of free radicals selectively damaging the pancreatic beta-cells. Since all the substances studied showed different positive effects, it is obvious that they have not acted only as a source of copper(II) ions but their effect is related to their specific chelate structure. The obtained results are a contribution to the knowledge of copper(II) Schiff base complexes with ligands of aldimine or ketimine type and form the basis for further preclinical tests of these bioactive agents in biological models of oxidative stress. 相似文献
13.
Eleni K. Efthimiadou Nikos Katsaros Alexandra Karaliota George Psomas 《Inorganica chimica acta》2007,360(15):4093-4102
The neutral mononuclear copper(II) complexes with the quinolone antibacterial drugs, pipemidic acid and N-propyl-norfloxacin, in the presence or absence of nitrogen-donor heterocyclic ligands, 2,2′-bipyridine, 1,10-phenanthroline or 2,2′-dipyridylamine, have been prepared and characterized spectroscopically. The interaction of copper(II) with the deprotonated quinolone ligand leads to the formation of the neutral mononuclear complexes of the type [Cu(quinolone)2(H2O)] (1)–(2) while the presence of the N-donor ligand leads to the formation of the neutral mononuclear complexes of the type [Cu(quinolone)(N-donor)Cl] (3)–(8). In all the complexes, copper(II) is pentacoordinate having a distorted square pyramidal geometry. The electron paramagnetic resonance spectra of 1 and 2 are typical of mononuclear Cu(II) complexes, while for the mixed-ligands complexes 3–8 a mixture of dimeric and monomeric species is indicated. The interaction of the complexes with calf-thymus DNA has been investigated with diverse spectroscopic techniques and has shown that the complexes can be bound to calf-thymus DNA by the intercalative mode. The antimicrobial activity of the complexes has been tested on three different microorganisms. All the complexes show an increased biological activity in comparison to the corresponding free quinolone ligand. 相似文献
14.
Complex formation of rutin and quercetin with copper alters the mode of inhibition of ribonuclease A
Rutin and quercetin, both minor components of green tea and their Cu(II) complexes interact with Ribonuclease A (RNase A) in a novel way. The effects of rutin, quercetin and their copper complexes on the catalytic activity of the protein were investigated. Rutin shows an enhancement in the ribonucleolytic activity whereas the copper complexes and quercetin behave as non-competitive type inhibitors with K(i) values in the μM range. The secondary structural changes of RNase A in presence of the ligands were measured by circular dichroism and Fourier transform infrared spectroscopy. The binding parameters were obtained using a fluorescence quenching analysis. 相似文献
15.
The intensity of the chemiluminescence of unstimulated human neutrophils in the presence of luminol was used to investigate the effects of low-molecular-weight copper complexes at the cellular level. In different models (superoxide dismutase mimetic activity, inhibition of haematoporphyrin derivative/light-induced lysis of cells), the biological activity of the complexes exceeded the activity of the ligands alone. 相似文献
16.
Psomas G Tarushi A Efthimiadou EK Sanakis Y Raptopoulou CP Katsaros N 《Journal of inorganic biochemistry》2006,100(11):1764-1773
The neutral mononuclear copper complexes with the quinolone antibacterial drug oxolinic acid in the presence or not of a nitrogen donor heterocyclic ligand 1,10-phenanthroline, 2,2'-bipyridine or 2,2'-dipyridylamine have been synthesized and characterized with infrared, UV-visible and electron paramagnetic resonance spectroscopies. The experimental data suggest that oxolinic acid acts as a deprotonated bidentate ligand and is coordinated to the metal ion through the pyridone and one carboxylate oxygen atoms. The crystal structure of (chloro)(1,10-phenanthroline)(oxolinato) copper(II), 2, has been determined with X-ray crystallography. For all complexes a distorted square pyramidal environment around Cu(II) is suggested. The EPR (electron paramagnetic resonance) behavior of 2 in aqueous solutions indicates mixture of dimeric and monomeric species. The investigation of the interaction of the complexes with calf-thymus DNA has been performed with diverse spectroscopic techniques and showed that the complexes are bound to calf-thymus DNA. The antimicrobial activity of the complexes has been tested on three different microorganisms. The complexes show a decreased biological activity in comparison to the free oxolinic acid. 相似文献
17.
E M Treshchalina N V Matve?chuk N O Gudratov G I Potapova 《Biulleten' eksperimental'no? biologii i meditsiny》1985,99(1):86-88
There was no direct inhibition of DNA synthesis in ascites hepatoma 22A cells after intraperitoneal injection of single doses of copper (II) complexes with amino acids into tumor-bearing C3HA mice. Meanwhile cis-dichlorodiamine platinum (II) (DDP) as well as sarcolysine showed such inhibition. Copper (II) complexes with alpha-amino acids displayed as significant superoxide dismutase-like activity at concentrations corresponding to therapeutic doses of these compounds. The complexes of copper (II) combined with DDP give an additive antitumor effect in solid tumors of mice. 相似文献
18.
Singh K Singh DP Barwa MS Tyagi P Mirza Y 《Journal of enzyme inhibition and medicinal chemistry》2006,21(5):557-562
Antibacterial Schiff bases derived from 1,2,4-triazoles as well as their metal complexes incorporating cobalt(II), nickel(II), copper(II) and zinc(II) have been synthesized and characterized. Physico-chemical studies suggest that an octahedral geometry for the cobalt(II), nickel(II) and zinc(II)and square-planer geometry for the copper(II) complexes. These complexes have been screened for antibacterial activity against three Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis) and two Gram-negative (Salmonella typhi and Pseudomonas aeruginosa) bacterial strains, and results compared with the activity of the free ligands. The metal complexes were found to be more potent against one or more bacterial strains than the free ligands. 相似文献
19.
Novel 2-oxo-1,2-dihydroquinoline-3-carbaldehyde (4′-methylbenzoyl) hydrazone (H2L) (1) and its two copper(II) complexes have been synthesized. Single-crystal X-ray diffraction studies revealed that the structure
of the new copper(II) chloride complex, [Cu(H2L)Cl2]·2H2O (2), is square pyramidal and that of the copper(II) nitrate complex, [Cu(HL)NO3]·DMF (3), is square planar. In 2, the copper atom is coordinated by the ligand with ONO donor atoms, one chloride ion in the apical position, and the other
chloride in the basal plane. In 3, the ligand coordinates as a uninegative tridentate ONO− species and with one nitrate ion in the basal plane. DNA binding experiments indicated that the ligand and copper(II) complexes
can interact with DNA through intercalation. Bovine serum albumin binding studies revealed that the compounds strongly quench
the intrinsic fluorescence of bovine serum albumin through a static quenching process. Antioxidative activity tests showed
that 1 and its copper(II) complexes have significant radical scavenging activity against free radicals. Cytotoxic activities of
the ligand and copper(II) complexes showed that the two copper(II) complexes exhibited more effective cytotoxic activity against
HeLa and HEp-2 cells than the corresponding ligand. The entire biological activity results showed that the activity order
was 1 < 2 < 3. 相似文献
20.
Sandbhor U Padhye S Billington D Rathbone D Franzblau S Anson CE Powell AK 《Journal of inorganic biochemistry》2002,90(3-4):127-136
N1-Benzylidene-pyridine carboxamidrazones and their metal conjugates have emerged as a new class of potential antimycobacterial agents. Nine such carboxamidrazone analogs (L1–L9) along with their Cu(II) (MC1–MC9) and Fe(III) (MC10–MC18) complexes were synthesized. Single crystal X-ray structures of copper complexes MC1 and MC5 were determined which suggest slightly distorted square planer geometries for copper complexes and octahedral geometries for ferric compounds. All compounds were evaluated for their in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv. The results show 32–64-fold enhancement in antitubercular activity upon copper complexation. 相似文献