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Cellular aspects of tolerance. V. The in vivo cooperative role of accessory and thymus derived cells in responsiveness and unresponsiveness of SJL mice

M Fujiwara B Cinader 《Cellular immunology》1974,12(2):194-204

Adult (8-week-old) SJL mice reach a relatively low degree of tolerance when injected with aggregate free rabbit γ-globulin (RGG). To analyze this phenomenon, we first examined indirect plaque-forming responses (PFC) in terms of participation of accessory and thymus-derived cells. Double transfer experiments were used; accessory cells were removed from donor cells by filtration over glasswool and their capacity reduced in recipients by 3 day preirradiation or by horse erythrocyte-mediated blockage. Using this type of experimental arrangement we found that the antibody response to RGG required the cooperation of accessory and thymus-derived cells. The induction of tolerance was affected by the presence of accessory cells. Preirradiated secondary recipients were reconstituted with spleen cells from accessory cell-deprived donors which had received thymus and bone marrow cells. In some experiments, the thymus and bone marrow cells were passed over glasswool. The primary recipients were left untreated or were given tolerogen. A more profound state of tolerance (reduction in plaque forming response) was the consequence of the incapacitation or removal of accessory cells. The magnitude of the reduction in PFC was directly related to the completeness of accessory cell removal and incapacitation. Responsiveness could be restored by administration of irradiated spleen cells as a source of accessory cells. The need for thymus-derived (T) cells in the antibody response was demonstrated by double transfer experiments in which the primary recipient was restored with thymus cells alone, bone marrow cells alone, or with a mixture of cell types. 相似文献

Cellular aspects of tolerance. VII. Inheritance of the resistance to tolerance induction 总被引：1，自引：0，他引：1

M Fujiwara B Cinader 《Cellular immunology》1974,12(2):214-229

The half-lives of elimination (

T_{12}

) of ¹³¹I-RGG from the body of normal A or Balb/c animals was much longer than the

T_{12}

of SJL mice. At all ages, the

T_{12}

of normal hybrids (A × SJL, SJL × A, Balb/c × SJL) was similar to or longer than that of the A or Balb/c parents. Thus, in terms of the

T 12

of normal animals, the SJL responsiveness to ¹³¹I-RGG appeared to be a recessive trait. Tolerance could be induced in newborn animals and, in terms of

T_{12}

, the degree of unresponsiveness at the age of 6 weeks, was the same in A, Balb/c, A × SJL, and Balb/c × SJL animals but was much shorter in SJL mice. Thus, in neonatally induced tolerance, the duration of tolerance was recessive for the SJL type. The average

T_{built12}

after tolerance induction in 3-week-old hybrids (A × SJL, SJL × A, Balb/c × SJL) was similar to that of the A or Balb/c parent, but by the 8th and 12th week it approached the average

T_{12}

of the SJL parent. Comparing 8-week-old hybrids, the average

T_{12}

was longest in A × SJL hybrids and identical in SJL × A and Balb/c × SJL mice. An examination of

T_{12}

distribution in various 8- and 12-week-old crosses and backcrosses revealed a fairly large proportion of individuals with a

T_{12}

which was intermediate between SJL and the other parent. There was a tendency for this number to decrease in 12 weeks as compared to 8-week-old mice. In 8-week-old mice, the number of animals with intermediate

T_{built12}

was smallest when SJL was the maternal animal [(SJL × A); SJL × (A × SJL); SJL × (SJL × A)]. There was no link between

T_{12}

of tolerant animals and either the immunoglobulin allotype (MuAl/MuA2) or the C5 eniotype (MuB1 positive/MuB1 negative). 相似文献