Lysimachia vietnamensis and L. verbascifolia spp. nov. (Primulaceae) from Vietnam

Two new species of Lysimachia (Primulaceae), L. vietnamensis L. K. Phan & C. M. Hu and L. verbascifolia C. M. Hu & K. L. Phan from Vietnam are described and illustrated.     

中国东部和南部小檗属植物之研究

我国小檗属植物种类十分丰富,据文献记载共有200余种,主要分布于西部和南部地区。东部和南部种类虽然较少,但迄今尚未见有较全面的整理和报导,故名称亦混乱。作者在编写《广东植物志》的过程中,对这一地区(包括广西、广东、湖南、江西、安徽、江苏、浙江、福建)的小檗属进行了初步的研究,发现了一些新分类群和分布新纪录,归并了B.chekiangensis Ahrendt和B.pingjiangensis Q.L.Chen et B.M.Yang,已知这一地区共有14种,2亚种和1变种。现将结果整理成文,内容包括已知种类的检索表、产地、分布以及对部分种类的评注和讨论。文中所引证的标本,凡未加标本室缩写代号者,均存华南植物研究所标本室。     

齿裂菌属的两个新种

本文报道齿裂菌属(Coccomyces de Not.)的两个新种一贵州齿裂菌(C.guizhouensis Y.R.Lin et B.F.Hu)和油杉齿裂菌(C.keteleeriae Y.R.Lin)。前者发生在贵州省织金县华山松(Pinus armandi Franch.)的枝条上,后一种见于福建省福州市油杉[Ketelecria fortunei(Murr.)Carr.]的落叶上。对这两个种作了汉文、拉丁文描述和图解。     

Ardisia banaensis and A. sadirioides spp. nov. (Myrsinaceae) from Vietnam

Two new species of Ardisia (Myrsinaceae), A. banaensis C. M. Hu & L. K. Phan and A. sadirioides C. M. Hu & L. K. Phan, from Vietnam are described and illustrated. Their distribution, habitat, phenology and closely related species are discussed.     

Revisions of the Genera Lathraea and Christisonia (Orobanchaceae) in China

The present paper deals with taxonomic studies on the genera Lathraea Linn. and Christisonia Gard. (Orobanchaceae) in China. In the genus Lathraea, only a single species in China, L. japonica Miq., is recongnized in the paper. It occurs in southern Shaanxi, southern Gansu, southeastern Sichuan, northern Guizhou and northern Guangdong, also in Japan. L. miqueliana Franch. et Sav., L. chinfushanica Hu et Tang and L. japonica Miq. var. miqueliana (Franch. et Sav.) Ohwi are treated as new synonyms of L. japonica Miq. in this paper. In the genus Christisonia, the most of collections from China, which were identified as C. sinensis G. Beck before, are treated as a new synonym of C. hookeri C. B. Clarke. A few collections of this genus from China, which were mistakenly described as Gleadovia lepoense Hu snd G. kwangtungense Hu, should be transferred to Christisonia hookeri C. B. Clarke. It is distributed in southeastern and south China, westwards to the Himalayas.     

悬钩子属植物的RAPD分析

用RAPD标记技术对13个黑莓（blackberry）、树莓（raspberry）品种和5个野生悬钩子种类（Rubus spp．）的26个居群的遗传多样性进行了分析。用15个寡聚核苷酸随机引物共扩增出条带131条,其中多态性条带118条,占扩增条带总数的90％,表明悬钩子属植物种间和品种间存在丰富的遗传多样性。通过聚类分析可将供试的26个居群分为4组,其中A组有粗叶悬钩子（R．alceaefolius Poir．）、山莓（R．corchorifolius L．）、插田泡（R．coreanus Miq．）、掌叶覆盆子（R．chingii Hu）、‘威廉姆特’、‘托拉咪’、‘泰勒’、‘金克维’、‘布里斯托’、‘宝森’7号、‘布莱兹’及其实生苗、‘基奥瓦’、‘萨尼’、‘黑布特’等15个居群;B组包括‘宝森’1号～‘宝森’6号和‘马林’、‘乔克多’等8个居群;C组包括‘赫尔’和‘切斯特’的2个居群;D组仅有蓬藁（R．hirsutus Thunb．）1种。这一聚类结果与传统形态学分类结果基本吻合。     

几种野生悬钩子家化的生物学基础研究

在对中国7个省区悬钩子属（Rubus L.）资源调查的基础上,选出蓬（R.hirsutus Thunb.）、掌叶覆盆子（R.chingii Hu）、山莓（R.corchorifolius L.）和高粱泡（R.lambertianus Ser.)4个野生种进行栽培化研究。本文报道4个种的生长结果习性、物候期、营养需要、花芽分化过程以及繁殖习性,并提出了一系列栽培措施。     

Enumeration and identification of IS3 elements in Escherichia coli strains.

Escherichia coli K-12 strains ordinarily contain five IS3 elements. Three of these correspond to previously mapped IS3 elements (R. C. Deonier, G. R. Oh, and M. Hu, J. Bacteriol. 129:1129--1140, 1977; S. Hu, E. Ohtsubo, and N. Davidson, J. Bacteriol. 122:749--763, 1975), and two additional IS3 elements are identified. The distribution of IS3 elements among deoxyribonucleic acid fragments generated by digestion with EcoRI indicates a basic pattern from which deviation is detected.     

拟单性木兰属（木兰科）植物的分类学修订

根据标本研究和野外调查,对木兰科(Magnoliaceae)的拟单性木兰属(Parakmeria Hu &; Cheng)进行了分类学修订。回顾了此属的分类学简史,阐述了保留拟单性木兰属的理由,将Magnolia Linn. subgenus Gynopodium Figlar &; Noot. section Gynopodium 作为拟单性木兰属的新异名,将Magnolia yunnanensis (Hu) Noot. 和M. nitida W. W. Smith var. robusta B. L. Chen &; Noot.作为云南拟单性木兰(Parakmeria yunnanensis Hu)的新异名,将Magnolia omeiensis (Cheng) Dandy、M. lotungensis Chun &; C. H. Tsoong、M. nitida W. W. Smith var. lotungensis (Chun &; C. H. Tsoong) B. L. Chen &; Noot. 和Parakmeria lotungensis (Chun &; C. H. Tsoong) Law作为峨眉拟单性木兰(Parakmeria omeiensis Cheng)的新异名,确认拟单性木兰属含４种植物,列出了分种检索表,描述了各种的地理分布和生长环境。     

中国11个维管束植物分类群名称原白中错误信息之更正

根据《国际藻类、菌物和植物命名法规》（深圳法规）条款9.2的要求,对中国11个维管束植物分类群名称原白中主模式指定的错误信息做了更正,这些名称是绒毛叶轮木（Holotype of Ostodes kuangii Y. T. Chang）、披针叶鹅耳枥（Holotype of Carpinus lancilimba Hu）、滇马蹄果（Holotype of Santiria yunnanensis Hu）、长尾观音座莲（Holotype of Angiopteris caudipinna Ching）、长萼黄芪（Holotype of Astragalus longicalyx C. C. Ni & P. C. Li）、越南油茶（Holotype of Camellia vietnamensis T. C. Huang ex Hu）、宽叶丛茎滇紫草（Holotype of Onosma waddellii f. latifolium W. T. Wang）、细齿堇菜（Holotype of Viola microdonta C. C. Chang）、糙叶毛蕨（Holotype of Cyclosorus scaberulus Ching）、两型叶乳源槭（Holotype of Acer chunii subsp. dimorphophyllum Fang）和南荻（Holotype of Miscanthus lutarioriparius L. Liu ex S. L.Chen & Renvoize）。这些名称的主模式标本均收藏在中国科学院植物研究所国家植物标本馆（PE）。     

金花茶拉丁名要更改

<正> 在中山大学学报(自然科学版)第30卷第3期(1991)上,张宏达教授和叶创兴先生发表了一篇研究报告,报道他们在研究山茶属(Camellia)植物当中,发现金花茶原植物早在四十年代已为戚经文先生定为新种,命名为Camellia nitidissima Chi,发表在国立中山大学理学院植物研究所专刊(Sunyatsonia)第了卷第1—2期上,19—20页,图版5。根据国际植物命名法规关于优先律的条款,对1965年胡先骗教授命名为 Theopsis chrysantha     

贵州植物区系增补

本文报道了贵州种子植物地理分布新记录属6个,新记录种14个,新记录变种1个。     

REVIEWS

Advances in Plant Nutrition, Vol. 2. Edited by B ernard T inker and A ndré L äuchli .
Molecular Biology and Crop Improvement. By R. B. A ustin with R. B. F lavell , I. E. H enson and H. J. B. L owe .
Crop Productivity – Research Imperatives Revisited (International Conference held at Boyne Highlands Inn, 1–318 October 1985 and Airlie House, 11–13 December, 1985). Edited by M artin G ibbs and C arla C arlson
Haploids of Higher Plants in Vitro. Edited by H. Hu and H.-Y. Y ang
Die Blüte. Eine Einführung in Struktur und Funktion, Ökologie und Evolution der Blüten. By D ieter H ess .
Mechanisms of Resistance to Plant Diseases. Advances in Agricultural Biotechnology, Volume 17. Edited by R. S. S. F raser .
British Fungus Flora, Volume 4, Pluteaceae: Pluteus and Volvariella. By P. D. O rton .
Nuclear Magnetic Resonance. Modern Methods of Plant Analysis, New Series, Volume 2. Edited by H. F. L insjens and J. F. J ackson .
Immunology in Plant Science. Edited by T revor L. W ang .     

Differential scanning calorimetric study of the thermal unfolding of mutant forms of phage T4 lysozyme.

In two recent papers, we reported the effects of several point mutations on the thermodynamics of the thermal unfolding of the lysozyme of phage T4 as determined by differential scanning calorimetry. The mutants studied were R96H [Kitamura, S., & Sturtevant, J.M. (1989) Biochemistry 28, 3788-3792] and T157 replaced by A, E, I, L, N, R, and V [Connelly, P., Ghosaini, L., Hu, C.-Q., Kitamura, S., Tanaka, A., & Sturtevant, J.M. (1991) Biochemistry 30, 1887-1891]. Here we report the results of a similar study of the single mutations A82P, A93P, and G113A and the double mutation C54T:C97A. The three single mutants all show small apparent stabilization at pH 2.5 and 46.2 degrees C (the denaturational temperature of the wild-type protein), amounting to -0.5 +/- 0.4 kcal mol-1 in free energy, whereas the double mutant shows a weak apparent destabilization, +0.8 +/- 0.4 kcal mol-1. As in all our previous studies of mutant proteins, the enthalpy changes produced by these mutations are in general of much larger magnitude than the corresponding free energy changes and frequently of opposite sign.     

Construction and characterization of a functional chimeric murine-human antibody directed against human fibrin fragment-D dimer

Fibrin-directed monoclonal antibodies may be clinically useful for in vitro thrombus imaging and for the targeting of fibrinolytic agents to blood clots. One such murine monoclonal antibody, (mAb-15C5), raised against the fragment-D dimer epitope of cross-linked human fibrin, was previously characterized [Holvoet, P., Stassen, J. M., Hashimoto, Y., Spriggs, D., Devos, P. & Collen, D. (1989) Thromb. Haemostasis 61, 307-313] has recently been cloned and expressed [Vandamme, A.-M., Bulens, F., Bernar, H., Nelles, L., Lijnen, H. R. & Collen, D. (1990) Eur. J. Biochem. 192, 767-775]. In order to reduce the immunogenicity of the murine mAb-15C5 in man, we have now constructed a murine--human chimera of mAb-15C5, by substituting the cDNA sequences encoding the constant regions of the murine kappa light chain and gamma 1 heavy chain by the corresponding human genomic sequences. Both chimeric murine--human Ig chains were cloned into two separately selectable expression vectors, which were contransfected into Chinese hamster ovary (CHO) cells. Murine--human chimeric mAb-15C5 (mAb-15C5Hu) was purified from the conditioned medium of selected cell lines by chromatography on Zn-chelating Sepharose, protein-A-Sepharose and on insolubilized antigen (fragment-D dimer), with a final yield of 29 micrograms/l and a recovery of 33%. SDS/PAGE without reduction revealed a homogeneous band with a mobility similar to that of natural mAb-15C5, whereas after reduction, both the heavy and the light chains had slightly slower mobilities than their natural counterparts. Expression in the presence of tunicamycin suggested that the differences in gamma 1-chain mobility were due to different N-glycosylation patterns. Immunoblotting of proteins from SDS gels showed immunological reactivity of recombinant mAb-15C5Hu with goat anti-(human IgG) IgG and of recombinant and natural murine mAb-15C5 with goat anti-(mouse IgG) IgG. Competitive binding revealed a comparable affinity of recombinant murine mAb-15C5, recombinant mAb-15C5Hu and natural mAb-15C5, for fragment-D dimer, indicating that recombinant mAb-15C5Hu was obtained in a functionally intact form. Thus, mAb-15C5Hu may constitute a useful alternative to mAb-15C5 for in vivo use in man.     

广西杜鹃花研究(二)

<正> 1.广西杜鹃 新种 图1 (映山红亚属,映山红组) 灌木,高2米;小枝纤细,被平贴铁锈色糙伏毛,后浙无毛。与紫花杜鹃 Rh.mar iae Hance有近缘,但叶薄革质,侧脉在背面部份隐晦,花冠无斑点,裂片长圆形,有凸尖,雄蕊常4枚,易于区别。     

西藏苔藓新植物

本种与多蒴异齿藓R．orthostegius相似,但区别为蒴柄平滑,叶边有单细胞锐齿,中肋达于叶尖稍下处终止。     

中国壳斗科柯属一新记录及二新异名

通过对中国主要标本馆壳斗科柯属标本的整理,发现一中国分布新记录种防城柯。基于标本形态学特征将光果柯和川柯分别作为硬壳柯和圆锥柯的异名进行归并。     

广西金花茶花粉形态

<正> 本文报道广西金花茶(Camellia Sect.Chrysanthae)8个种1个变种花粉在光学显微镜和电子扫描显微镜下观察的结果。 材料和方法 材料取自在广西采集的腊叶标本上的花粉。制片方法采用G.Erdtman的醋酸酐分解法,在光学显微镜下测量其大小。扫描电镜观察材料采用醋酸酐分解,后用蒸馏水洗净,再用纯酒精脱水,上铜台,喷金,在扫描电子显微镜下观察并照相。