Non-alkaloid Constituents of Buxus sinica (Buxaceae)

From the aerial parts of Buxus sinica, ten non- alkaloid compounds were isolated and identified as cleomiscosin A ( 1 ), 3, 5-dihydroxyl-4', 6, 7-trimethyl flavone-3′- O-β- D-glucopyranoside ( 2 ), 3, 5, 3', 4′tetrahydroxyl-3, 6, 7-trimethyl flavone (3 ) , cleomiscosin A-4′- O-β- D-gluco-pyranoside (4 ), 3, 5-dimethoxybenzoicacid-4- O-β- D-glucopyranoside (5 ), 4′, 5-dihydroxyl-3, 6, 7-trimethyl flavone (6), lupine (7), ( + )-pinoresinol- O-β- D-glucopyranoside (8 ), β-sitosterol (9), and daucosterol (10). Their structures were identified by spectral evidence.     

云南产玉米须的化学成分研究

从玉米须60%乙醇提取液中分离得到了14个化合物,经波谱学方法分别鉴定为柯伊利素-7-O-β-D-葡萄糖苷(1)、柯伊利素-6-C-β-波伊文糖-7-O-β-葡萄糖苷(2)、柯伊利素-6-C-β-波伊文糖苷(3)、L-鼠李糖(4)、豆甾-4-烯-3β,6β-二醇(5)、7α-羟基谷甾醇(6)、7β-羟基谷甾醇(7)、胡萝卜苷棕榈酸酯(8)、大豆脑苷I(9)、7α-羟基谷甾醇-3-O-β-D-葡萄糖苷(10)、麦角甾-7,22-二烯-3β,5α,6β-三醇(11)、棕榈酸(12)、胡萝卜苷(13)和β-谷甾醇(14),其中化合物1、4和7～12为首次从玉米须中分离得到。     

地菍的化学成分(英文)

为了研究野牡丹科药用植物地菍(Melastoma dodecandrum)的化学成分,采用柱层析方法分离鉴定了15个化合物,通过波谱分析及对比文献等方法鉴定为4-O-β-D-吡喃葡萄糖基-3,3',4'-三甲氧基鞣花酸(1),槲皮素3-O-刺槐二糖苷(2),8-C-吡喃葡萄糖基-5,7,3',4'-四羟基黄酮(3),3-O-β-D-吡喃葡萄糖基-4',5,7-三羟基黄酮(4),6-C-吡喃葡萄糖基-4',5,7-三羟基黄酮(5),3-hydroxy-22(29)-hopen-23-oic acid(6),2,3-dihydroxy-9(11)-fernen-23-oic acid(7),3β-sitosterol laminaribioside(8),姜糖酯B(9),3-O-β-D-galactopyranoside-glycerol1-alkanoates(10),胡萝卜苷(11),β-谷甾醇(12),二十八烷醇(13),二十四烷酸(14)以及三十四烷(15)化合物结构。所有化合物均为首次从该植物中分离得到。     

菜蕨的化学成分研究

采用硅胶柱层析和凝胶柱层析对菜蕨的丙酮提取物进行分离纯化,首次从中分离得到10个化合物,通过波谱学数据并和已知化合物数据比较,鉴定它们分别为β-sitosterol(1),Stigmast-4-ene-6β-ol-3-one(2),Stigmast-4-ene-3,6-dione(3),Benzeneacetic acid(4),3β-Hydroxy-5α,8α-epidioxyergosta-6,22-diene(5),Stigraast-4-ene-3β,6β-diol(6),Stigmast-5-ene-3β,7α-diol(7),Stigmast-4-ene-6α-ol-3-one(8),Glycerol-1,3-dihexadecanoate(9)以及Daucosterol(10).     

连钱草化学成分研究

从连钱草(Glechoma longituba)分离并鉴定了10个化合物,分别为β-谷甾醇(1)、齐敦果酸(2)、熊果酸(3)、2α,3α-二羟基乌苏-12-烯-28-酸(4)2、α,3β-二羟基乌苏-12-烯-28-酸(5)、胡萝卜苷(6),3,6-二甲氧基-6″,6″-二甲基苯并吡喃-(7,8,2″,3″)-黄酮(7)、芫花素(8)、芹菜素-7-O--βD-葡萄糖苷(9)、木犀草素-7-O-β-D-葡萄糖甙(10)。其中化合物2,4~8首次从该植物中分离得到。     

Synthesis of blockwise alkylated (1→4) linked trisaccharides as surfactants: influence of configuration of anomeric position on their surface activities

New carbohydrate-based surfactants consisting of hydrophilic cellobiosyl and hydrophobic glucosyl residues, methyl β-d-glucopyranosyl-(1→4)-α-d-glucopyranosyl-(1→4)-2,3,6-tri-O-methyl-α-d-glucopyranoside 1 (GβGαMα, G: glucopyranosyl residue, α and β: α-(1→4)- and β-(1→4) glycosidic bonds, M: methyl group), 2 (G(β)G(β)M(α)), 3 (G(β)G(α)M(β)), 4 (G(β)G(β)M(β)), 5 (G(β)G(α)E(α), E: ethyl group), 6 (G(β)G(β)E(α)), 7 (G(β)G(α)E(β)), 8 (G(β)G(β)E(β)) and eight α-and β-glycoside mixtures (a mixture of 1 and 2: 1/2=62/38 (9), 32/68 (10); a mixture of 3 and 4: 3/4=69/31 (11), 32/68 (12); a mixture of 5 and 6: 5/6=62/38 (13), 33/67 (14); a mixture of 7 and 8: 7/8=59/41 (15), 29/71 (16)) were synthesized via combined methods consisting of acid-catalyzed alcoholysis of cellulose ethers and glycosylation of phenyl thio-cellobioside derivatives. Their surface activities in aqueous solution depended on their chemical structures: α- or β-(1→4) linkage between hydrophilic cellobiosyl and hydrophobic glucosyl blocks, methyl or ethyl groups of hydrophobic glucosyl block, and α- or β-linked ether group at the C-1 of hydrophobic glucosyl block. The mixing effect of α- and β-glycosides on surface activities was also investigated. As a result, ethyl β-d-glucopyranosyl-(1→4)-α-d-glucopyranosyl-(1→4)-2,3,6-tri-O-ethyl-β-d-glucopyranoside 7 (G(β)G(α)E(β)) had the highest surface activity, and its critical micellar concentration (CMC) and γ(CMC) (surface tension at CMC) values of compound 7 were 0.5mM (ca. 0.03wt%) and 34.5mN/m, respectively. The surface tensions of α- and β-glycoside mixtures except for compounds 9 and 10 were almost equal to those of pure compounds. The syntheses of the mixtures of α- and β-glycosides without purification process are easier than those of pure compounds. Thus, the mixtures should be more practical compounds for industrial use as a surfactant.     

牛心朴子须根的化学成分研究

从采自宁夏的萝摩科鹅绒藤属植物牛心朴子 (CynanchumkomaroviiAl.Iljinski.)须根的乙醇提取物中分离鉴定了十个非C2 1 甾体类化合物 :β D 呋喃果糖基 (2→ 1) α D [6 O 芥子酰基 ] 吡喃葡萄糖甙 (1) ,β D (3 O 芥子酰基 ) 呋喃果糖基 (2→ 1) α D [6 O 芥子酰基 ] 吡喃葡萄糖甙 (2 ) ,[6 O β D 吡喃葡萄糖基 (1→ 6 ) β D 吡喃葡萄糖基 1,2 双氧 (4 羟基 3,5 二甲氧基肉桂酰 ) (3) ,7 脱甲氧基娃儿藤碱 (4) ,9 羟基 芳樟醇 3 O β D 吡喃木糖基 (1→ 6 ) β D 吡喃葡萄糖甙 (5 ) ,(2E ,6R) 2 ,6 二甲基 2 ,7 辛二烯 1,6 二醇 (6 ) ,[(+) 丁香素 ](7) ,4′ O demethylepiyangambin(8) ,4′ 羟基 2′ 甲氧基苯乙酮 (9) ,(2S ,3S ,4R ,12E) N [2′ (R) 羟基二十二碳烷基 ] 1,3,4 三羟基 2 酰胺 二十碳烷基 12 烯 (10 )。除化合物 4和 9外 ,其余化合物均为首次从该植物中分离得到。     

Biotransformation of dehydroepiandrosterone with Macrophomina phaseolina and β-glucuronidase inhibitory activity of transformed products

The biotransformation of dehydroepiandrosterone (1) with Macrophomina phaseolina was investigated. A total of eight metabolites were obtained which were characterized as androstane-3,17-dione (2), androst-4-ene-3,17-dione (3), androst-4-ene-17β-ol-3-one (4), androst-4,6-diene-17β-ol-3-one (5), androst-5-ene-3β,17β-diol (6), androst-4-ene-3β-ol-6,17-dione (7), androst-4-ene-3β,7β,17β-triol (8), and androst-5-ene-3β,7α,17β-triol (9). All the transformed products were screened for enzyme inhibition, among which four were found to inhibit the β-glucuronidase enzyme, while none inhibited the α-chymotrypsin enzyme.     

On the non-existence of eloxanthin

Abandonment of the name eloxanthin is proposed. The principal carotenoids in various species of Elodea were (3R, 3′R, 6′R)-lutein (β,ε-carotene-3, 3′-diol) and β, β-carotene. The minor pigments were neoxanthin-X (5′, 6′-epoxy-6, 7-didehydro-5, 6, 5′, 6′-tetrahydro-β, β-carotene-3, 5, 3′-triol), 9′-cis-neoxanthin- X, 9- and 13-cis-violaxanthin (5, 6, 5′, 6′-diepoxy-5, 6, 5′, 6′-tetrahydro-β, β-carotene-3, 3′-diol), antheraxanthin (5, 6-epoxy-5, 6-dihydro-β, β-carotene-3, 3′-diol), neolutein A (13- or 13′-cis-lutein) and neolutein B (9- or 9′-cis-lutein). All attempts to isolate eloxanthin failed.     

四川溲疏的化学成分研究(英文)

运用多种色谱技术从四川溲疏(Deutzia setchuenensis Franch)全草的95%乙醇提取物中首次分离到9个化合物。通过波谱方法和与已知品对照的手段将它们鉴定为:β-谷甾醇(1)、白桦酯醇(2)、hydrangetin(3)、齐墩果酸(4)、肉桂酸(5)、齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷(6)、β-胡萝卜苷(7)、齐墩果酸-3-O-(β-D-吡喃葡萄糖醛酸-6-正丁酯)(8)和齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸-28-O-β-D-吡喃葡萄糖苷(9)。     

Modulation of gain-of-function α6*-nicotinic acetylcholine receptor by β3 subunits

We previously have shown that β3 subunits either eliminate (e.g. for all-human (h) or all-mouse (m) α6β4β3-nAChR) or potentiate (e.g. for hybrid mα6hβ4hβ3- or mα6mβ4hβ3-nAChR containing subunits from different species) function of α6*-nAChR expressed in Xenopus oocytes, and that nAChR hα6 subunit residues Asn-143 and Met-145 in N-terminal domain loop E are important for dominant-negative effects of nAChR hβ3 subunits on hα6*-nAChR function. Here, we tested the hypothesis that these effects of β3 subunits would be preserved even if nAChR α6 subunits harbored gain-of-function, leucine- or valine-to-serine mutations at 9' or 13' positions (L9'S or V13'S) in their second transmembrane domains, yielding receptors with heightened functional activity and more amenable to assessment of effects of β3 subunit incorporation. However, coexpression with β3 subunits potentiates rather than suppresses function of all-human, all-mouse, or hybrid α6((L9'S or V13'S))β4*- or α6(N143D+M145V)(L9'S)β2*-nAChR. This contrasts with the lack of consistent function when α6((L9'S or V13'S)) and β2 subunits are expressed alone or in the presence of wild-type β3 subunits. These results provide evidence that gain-of-function hα6hβ2*-nAChR (i.e. hα6(N143D+M145V)(L9'S)hβ2hβ3 nAChR) could be produced in vitro. These studies also indicate that nAChR β3 subunits can be assembly partners in functional α6*-nAChR and that 9' or 13' mutations in the nAChR α6 subunit second transmembrane domain can act as gain-of-function and/or reporter mutations. Moreover, our findings suggest that β3 subunit coexpression promotes function of α6*-nAChR.     

唐古特瑞香的化学成分研究

从唐古特瑞香(Daphne tangutica Maxim)95%乙醇提取物石油醚部分分离得到14个化合物,通过波谱学方法鉴定为β-谷甾醇(1)、β-胡萝卜苷(2)、2,4-二羟基-3,6-二甲基苯甲酸甲酯(3)、蛇床子素(4)、植物醇(5)、软脂酸(6)、stigmast-5-ene-3β,7α-diol(7)、(-)-lariciresinol(8)、daphnetin(9)、daphnoretin(10)、(-)-丁香树脂醇(11)、( )-medioresinol(12)、daphnelicin(13)和咖啡酸二十二酯(14)。     

雪茶化学成分研究

由管枝衣科地茶属植物雪茶（Thamnolia vermicularis)丙酮提取物中分离得到10个成分,通过波谱分析及与已知品对照等方法,最终确定其中的9个化合物分别为:Thamnolin(1),鳞片衣酸（2）,坝巴酸（3）,羊角衣酸（4）,3β－羟基－5α,8α－桥二氧麦角甾－6,22－二烯（5）,3β－羟基－5α,8α－桥二氧麦角甾－6,9,22－三烯（6）,麦角甾烷－7,22－二烯－3－醇（7）,麦角甾烷－5,8,22－三烯－3－醇（8）,亚油酸(9)。其中,化合物1为新化合物,化合物3和6－9系首次由该种植物中分离得到。研究结果初步明确了云南中甸地区产雪茶为主要含有坝巴酸（3）和羊角衣酸（4）等酚性成分,而非主含地茶酸等酚性成分的植物品种类型,这为合理开发利用当地植物资源提供了科学依据。     

滇南蛇根草化学成分的研究

采用柱色谱技术从药用植物滇南蛇根草Ophiorrhiza austroyunanensis Lo.全草的甲醇提取物分离得到9个化合物,利用理化性质及波谱方法分别鉴定为β-谷甾醇(1)、β-豆甾醇(2)、β-胡萝卜苷(3)、白桦脂醇(4)、叶黄素(5)、东莨菪素(6)、熊果酸(7)、茜根酸-1-甲醚(8)和豆甾醇-3-O...     

Identification of N-terminal extracellular domain determinants in nicotinic acetylcholine receptor (nAChR) α6 subunits that influence effects of wild-type or mutant β3 subunits on function of α6β2*- or α6β4*-nAChR

Despite the apparent function of naturally expressed mammalian α6*-nicotinic acetylcholine receptors (α6*-nAChR; where * indicates the known or possible presence of additional subunits), their functional and heterologous expression has been difficult. Here, we report that coexpression with wild-type β3 subunits abolishes the small amount of function typically seen for all-human or all-mouse α6β4*-nAChR expressed in Xenopus oocytes. However, levels of function and agonist potencies are markedly increased, and there is atropine-sensitive blockade of spontaneous channel opening upon coexpression of α6 and β4 subunits with mutant β3 subunits harboring valine-to-serine mutations at 9'- or 13'-positions. There is no function when α6 and β2 subunits are expressed alone or in the presence of wild-type or mutant β3 subunits. Interestingly, hybrid nAChR containing mouse α6 and human (h) β4 subunits have function potentiated rather than suppressed by coexpression with wild-type hβ3 subunits and potentiated further upon coexpression with hβ3(V9'S) subunits. Studies using nAChR chimeric mouse/human α6 subunits indicated that residues involved in effects seen with hybrid nAChR are located in the α6 subunit N-terminal domain. More specifically, nAChR hα6 subunit residues Asn-143 and Met-145 are important for dominant-negative effects of nAChR hβ3 subunits on hα6hβ4-nAChR function. Asn-143 and additional residues in the N-terminal domain of nAChR hα6 subunits are involved in the gain-of-function effects of nAChR hβ3(V9'S) subunits on α6β2*-nAChR function. These studies illuminate the structural bases for effects of β3 subunits on α6*-nAChR function and suggest that unique subunit interfaces involving the complementary rather than the primary face of α6 subunits are involved.     

醉魂藤的化学成分研究

运用多种色谱技术从云南产醉魂藤Heterostemma alatum wight中分离得到10个化合物.通过理化鉴别和波谱数据确定了他们的化合物结构分别为β-谷甾醇(1)、正二十四烷酸(2)、芹菜素(3)、胡萝卜苷(4)、芹菜素-7-O-β-D-葡萄糖苷(5)、醉魂藤碱A(6)、醉魂藤碱B(7)、醉魂藤碱C(8)、醉魂藤碱D(9)和醉魂藤碱F(10).这些化合物均为首次从该植物中分离得到.     

紫花苜蓿化学成分的研究(英文)

为研究紫花苜蓿（Medicago scttiva L．）地上部位的化学成分,通过溶剂萃取、硅胶柱色谱、凝胶柱色谱分离纯化,从紫花苜蓿地上部分分离得到9个化合物,根据质谱和核磁共振数据鉴定为小檗碱（1）、大黄素（2）、大黄素8-O-β-D-葡萄糖苷（3）、soyαsαpogenol B-3-O-β-glcA（4）、邻羟基苯甲酸（5）、反式对羟基肉挂酸（6）、顺式对羟基肉桂酸（7）、正三十烷醇（8）和β-胡萝卜苷（9）。其中化合物1—4为首次从苜蓿属植物中分离得到。     

羊角天麻化学成分及β-羟高铁血红素形成抑制活性研究

为探究羊角天麻(Dobinea delavayi)的β-羟高铁血红素形成抑制活性成分,本实验采用硅胶、Sephadex LH-20柱色谱等方法进行化合物的纯化分离,通过理化性质及波谱数据鉴定化合物结构。结果从羊角天麻中分离得到12个化合物,分别鉴定为3,4,2′,4′-四羟基二氢查尔酮(1)、紫铆花素(2)、3,4,2′,4′,α-五羟基查尔酮(3)、金色草素(4)、芒果苷(5)、没食子酸(6)、二十四亚甲基环阿尔廷醇(7)、6β-羟基-豆甾-4-烯-3-酮(8)、β-谷甾醇(9)、胡萝卜苷(10)、棕榈酸甲酯(11)、1-棕榈酸单甘油酯(12),其中化合物1~4、6~8均为首次九子母属植物中分离得到。活性研究结果显示,化合物1~6均具有β-羟高铁血红素形成抑制活性,其中黄酮类化合物2~4具有较强的抑制活性,其IC50分别为120.3、61.5、56.0μg/mL。     

Chemical Constituents of Phacellaria compressa Benth.

Two new compounds, 1-（3-methoxy-4-hydroxyphenyl）-3-（3,5-dimethoxy-4-hydroxyphenyl）-propane （1） and 5, 7, 3＇-trlmethyoxyflavan-4＇-O-β-D-glucopyranoslde （2） were Isolated from the ethanol extract of the dried aerial parts of Phacellarla compressa Benth., together with 2,3-bis[（4-hydroxy-3,5-dimethoxyphenyl）-methyl]-1,4- butanedlol （3）, ethyl 3,4,5-trlhydroxybenzoate （4）, methyl 3, 4, 5-trlhydroxybenzoete （5）, β-sltoeterol （6）, 5, 7, 3＇, 4＇- tetrahydroxyfiavan （7）, lupeol （8）, zhebelreslnol （9）, quercetln-3-O-α-L-rhamnopyranoslde （10）, （＋）-cetechin （11）, betulln （12）, β-daucosterol （13）, （＋）-sydngareslnol （14）, scopoletln （15）, and proxlmadlol （16）. The structures of these compounds were determined by spectral evidence or by comparing them with authentic samples. Compound 9 showed α-amylase Inhibitory activity of 57.55% at a concentration of 50 μg/mL.     

蔓生百部的化学成分研究

从蔓生百部(Stemona japonica)根中分离得到13个化合物,通过波谱数据,它们鉴定为β-谷甾醇(1)、豆甾醇(2)、5,11-豆甾二烯-3β-醇(3)、苯甲酸(4)、4-甲氧基苯甲酸(5)、1,8-二羟基-3-甲基蒽醌(6)、1,8-二羟基-6-甲氧基-3-甲基蒽醌(7)、氧代狭叶百部碱(8)、百部定碱(9)、异狭叶百部碱(10)、绿原酸(11)、栀子苷(12)和藏红花素A(13).所有化合物均为首次从该植物中分离得到.