药敏药片对临床235株细菌实验观察报告

药敏药片经临床对金黄色葡萄球菌、大肠埃希氏菌、铜绿假单胞菌等２３５株考核．表明药片工艺研究先进,药片与培养基结合牢固,无断裂、崩解,不渗出颗粒,抑菌圈呈同心园扩散．边缘清楚。药物含量均匀,释放度好。药片抑菌差仅１～３ｍｍ;而纸片抑菌差为２～１２ｍｍ。药片变黑系数ＣＶ为２．７１～４．２１;而纸片ＣＶ为３．８２～１４．３６。表明纸片片间差大,药片精密度明显好于纸片。     

短柄五加大,小孢子发生和雌,雄配子体发育的研究

短柄五加花药５枚,每个花药四个花粉囊。小孢子母细胞减数分裂时,胞质分裂为同时型,产生正四面体形的四分体。花药壁由表皮、药室内壁、中层和绒毡层四层细胞组成,其发育类型为双子叶型。腺质绒毡层,其细胞为二核。三细胞型花粉。子房５室,每室两个胚珠,上胚珠败育,下胚珠可育。下胚珠倒生,具单珠被,厚珠心。大孢子母细胞减数分裂形成线性排列的四个大孢子,雌配子体发育属蓼型。开花当天,花粉散开,雌配子体尚未成熟,处     

沙冬青淀粉粒及其与叶绿体发育的关系

沙冬青叶绿体中的淀粉粒一般为１－３个,主要有４种类型。第１种外周部分电子密度较高,中央部分较低,但每个部分的电子密度十分均匀。它们近似椭圆形,附近的类囊体形态正常,结构清晰。第２种电子密度由外向内逐渐变低,多为椭圆形,附近的类囊体较清晰。第３种外周部分的电子密度很高,中央部分不均匀,形状多种多样,附近的类囊体有的不清晰。第４种电子密度很低,十分均匀,形状不规则,附近的类囊体已经解体或正在解体。分析表明,淀粉的形态变化明显与叶绿体发育有关。     

Failure to Maintain Glycolysis in Anoxic Nerve Terminals

Synaptosomal glycolysis is stimulated eight- to 10-fold when the respiratory chain is inhibited by cyanide or by anoxia. However, the stimulation is transient and after 15 min declines toward the preanoxic rate. The decline is not seen when Ca2+ is absent or when the respiratory chain is inhibited by rotenone. The decline in glycolysis is reversible, is not due to substrate exhaustion, and is the cause, rather than the effect, of lowered synaptosomal ATP/ADP ratios. The failure to maintain glycolysis when the terminal oxidase of the respiratory chain is inhibited may have relevance to the sensitivity of the brain to anoxic damage.     

糖、糖、“糖”——什么是糖?

糖类是自然界中最常见的一类生物分子,但是糖类因不同的人群可以有三种不同的理解.一是科学上的糖类,二是生活中的糖类,三是作为甜味剂的"糖"类.因为甜味是一个生理上的感觉,凡能与甜味受体相互作用的均是甜味剂,其中有些是糖的"糖",也有不是糖的"糖".     

杉木人工林水量平衡和蒸散的研究

 本文根据我们严密设计的小集水区径流场连续6年的水文测定数据,进行了杉木人工林水量平衡和蒸散的研究。结果表明：集水区年平均降雨量1065.5mm, 在林冠作用面降雨量的分配中,林冠截留雨量264.6mm,截留率24.8%;穿透过林冠层的雨量799.82mm,树干径流量1.08mm,分别占降雨量的75.1%和0.1%。林内降水到达林地时,在枯枝落叶层这个作用面上净降水进行再分配,其中,地表径流量9.27mm,地下径流量203.00mm,总径流系数0.199。土壤蓄水量月变化较大,但年变化很小,占降雨量的1.2%。系统水量最大的输出是蒸散,每年以气态形式返回大气的水量866.03mm,占降雨量81.3%。在蒸散的水量中,林冠截留雨量的直接物理蒸发量为264.6mm,占总蒸散量的31.6%。     

矮沙冬青雌配子体及胚胎发育研究

矮沙冬青子房单心皮1室,边缘胎座,弯生胚珠,胚珠具双珠被、厚珠心。大孢子孢原细胞发生于珠心表皮下,大孢子母细胞减数分裂形成直线排列的四分体,合点端大孢子具功能,并按蓼型胚囊发育,雌配子体成熟于4月中旬。双受精后,胚乳发育为核型。在矮沙冬青大孢子发生、雌配子体和胚胎发育过程中未发现异常现象,因此认为矮沙冬青濒危不存在雌性生殖结构与发育过程异常的内在因素。     

The consequences of dominance and gene flow for local adaptation and differentiation at two linked loci

For a subdivided population the consequences of dominance and gene flow for the maintenance of multilocus polymorphism, local adaptation, and differentiation are investigated. The dispersing population inhabits two demes in which selection acts in opposite direction. Fitness is determined additively by two linked diallelic loci with arbitrary intermediate dominance (no over- or underdominance). For weak as well as strong migration, the equilibrium structure is derived. As a special case, a continuous-time continent–island model (CI model) is analyzed, with one-way migration from the continent to the island. For this CI model, the equilibrium and stability configuration is obtained explicitly for weak migration, for strong migration, for independent loci, and for complete linkage. For independent loci, the possible bifurcation patterns are derived as functions of the migration rate. These patterns depend strongly on the degree of dominance. The effects of dominance, linkage, and migration on the amount of linkage disequilibrium (LD) and the degree of local adaptation are explored. Explicit formulas are obtained for $D$   $(=x_1{x}_4-x_2{x}_3)$ and $r^2$ (the squared correlation in allelic state). They demonstrate that dominant island alleles increase $D$ and decrease $r^2$. Local adaptation is elevated by dominance of the locally adaptive alleles. The effective migration rate at a linked neutral locus is calculated. If advantageous alleles are dominant, it is decreased only slightly below the actual migration rate. For a quantitative trait that is determined by two additive loci, the influence of dominance on measures of differentiation is studied. Explicit expressions for $Q_{ST}$ and two types of $F_{ST}$ at equilibrium are deduced and their relation is discussed.     

Vanadate-stimulated NADH oxidation in plasma membrane

The rate of NADH oxidation with oxygen as the acceptor is very low in mouse liver plasma membrane and erythrocyte membrane. When vanadate is added, this rate is stimulated 10- to 20-fold. The absorption spectrum of vanadate does not change with the disappearance of NADH. The reaction is inhibited by superoxide dismutase, and there is no activity under an argon atmosphere. This indicates that oxygen is the electron acceptor and the reaction is mediated by superoxide. The vanadate stimulation is not limited to plasma membrane. Golgi apparatus and endoplasmic reticulum show similar increase in NADH oxidase activity when vanadate is added. The endomembranes have significant vanadate-stimulated activity with both NADH and NADPH. The vanadate-stimulated NADH oxidase in plasma membrane is inhibited by compounds, which inhibit NADH dehydrogenase activity: catechols, anthracycline drugs and manganese. This activity is stimulated by high phosphate and sulfate anion concentrations.     

药物敏感试验药片法研究

药敏药片对国际三株标准菌试验,抑菌圈直径范围均符合判定标准。与进口药敏药片符合率为１００％。与药敏纸片片间差比较试验,药片抑菌圈＝１～２ｍｍ,精密度高,符合国际要求,而纸片抑菌圈＝９～１３ｍｍ,均匀度检测明显不合格。药片变异系数ＣＶ＝１．３１～２．９２,纸片ＣＶ＝１４．７８～２１．６７表明药片变量离散度小,片间差很小;而纸片变量离散度明显大,故纸片片间差很大。     

Discrete three-stage population model: persistence and global stability results

A general three-stage discrete-time population model is studied. The inherent net reproductive number for this model is derived. Global stability of the origin is established provided that the inherent net reproductive number is less than one. If it is larger than one the existence of a unique positive fixed point is proved and the persistence of the system is established. Finally, for certain parameter ranges global stability of the positive fixed point is proved.     

小球藻的异养生长及培养条件优化

对小球藻异养培养中的碳源、氮源、微量元素—镁离子以及其他培养条件的影响进行了探讨 ,并测定了小球藻的生长曲线。优化结果 :C∶ N为 4∶ 1～ 5∶ 1 ,硫酸镁的量为 1 g/L;培养条件为 :p H6～ 7,接种量1 0 % ,温度 3 0°C。在此条件下 ,异养培养小球藻 ,其 OD值可达 1 8,蛋白质为 3 0 % ,叶绿素含量为 1 .2 %。     

羚牛(Budorcas taxicolor)部分脏器特点的观察

本文对2只羚牛的雌性生殖器官及肝、肾、脾等脏器进行了形态描述,并与黄牛及羊的相应器官进行了比较,为探讨羚牛的分类地位提供了解剖学资料。     

Influx of γ-Aminobutyric Acid and α-Aminoisobutyric Acid into Mouse Cerebrum Slices Incubated in a Pyruvate Medium with or without Added Glucose or Glucose Analogues Compared with Influx from a Glucose Medium

Concentrative influx of γ-aminobutyric acid (GABA) and α-aminoisobutyric acid (AIB) into incubated mouse cerebrum slices is decreased when pyruvate is substituted for glucose. Influx of GABA from pyruvate medium is not increased by presence of glucose, 2-deoxy-d -glucose (2-DOG), or 3-O-methyl-d -glucose (3-O-MeG). Influx of AIB is restored to the rate from glucose medium if 2-DOG is present initially, but is not restored if 2-DOG is added with AIB. Influx is not restored if 3-O-MeG is present initially, but is restored if 3-O-MeG is added with AIB. Influx is restored if glucose is present initially or is added with AIB.     

清栓酶治疗脑血栓冠心病微循环变化初步观察

本文报导40例脑心血管疾病在清栓酶治疗前后进行甲皱微循环对比观察,其中脑血栓组20例,冠心病组20例,两组均在清栓酶治疗2～3疗程后复查,分别对其甲皱微循环微血管袢的形态及功能,血液流态和微血管周围变化等三方面进行治疗前后对比观察,结果发现,治疗前甲皱微循环改变是明显的,临床症状较重,治疗后甲皱微循环障碍均得到了明显改善,临床症状也随着减轻或消失。治疗前后比较,其差异有显著的意义 P<0.05,P<0.01.从临床对比观察所见,脑心血管疾病的微循环改变与病情有关,其改变与病情轻重相吻合,与疾病转归相一致,提示微血管病变是动脉硬化临床可识别的象征.微循环障碍与疾病的发生发展有着内在联系,是脑心血管疾病的病因和发病的中间环节.治疗后微循环障碍的明显改善,提示清栓酶有着较好的改善微循环的功能.     

Estimation of distal arm joint angles from EMG and shoulder orientation for transhumeral prostheses

In this paper, we quantify the extent to which shoulder orientation, upper-arm electromyography (EMG), and forearm EMG are predictors of distal arm joint angles during reaching in eight subjects without disability as well as three subjects with a unilateral transhumeral amputation and targeted reinnervation. Prior studies have shown that shoulder orientation and upper-arm EMG, taken separately, are predictors of both elbow flexion/extension and forearm pronation/supination. We show that, for eight subjects without disability, shoulder orientation and upper-arm EMG together are a significantly better predictor of both elbow flexion/extension during unilateral ($R^2=0.72$) and mirrored bilateral ($R^2=0.72$) reaches and of forearm pronation/supination during unilateral ($R^2=0.77$) and mirrored bilateral ($R^2=0.70$) reaches. We also show that adding forearm EMG further improves the prediction of forearm pronation/supination during unilateral ($R^2=0.82$) and mirrored bilateral ($R^2=0.75$) reaches. In principle, these results provide the basis for choosing inputs for control of transhumeral prostheses, both by subjects with targeted motor reinnervation (when forearm EMG is available) and by subjects without target motor reinnervation (when forearm EMG is not available). In particular, we confirm that shoulder orientation and upper-arm EMG together best predict elbow flexion/extension ($R^2=0.72$) for three subjects with unilateral transhumeral amputations and targeted motor reinnervation. However, shoulder orientation alone best predicts forearm pronation/supination ($R^2=0.88$) for these subjects, a contradictory result that merits further study.     

扬子鳄视觉器官组织学研究

本文用光镜和电镜研究了扬子鳄(Alligator sinensis)的组织学,同时测量了其眼球的一些光学参数.扬子鳄眼球呈扁圆球形,角膜径与球径的比值为1:1.44;晶状体与角膜的比值为1:1.40。角膜内具鲍氏膜;虹膜内的括约肌、睫状体内的睫状肌均属横纹肌,视细胞椭圆体内线粒体嵴突与线粒体长轴相平行,这与报道的其它鳄类不同。虹膜内未见扩瞳肌纤维,角膜缘缺巩膜小骨片,晶状体环垫薄,因而其视觉调节能力仍然很弱。视网膜中视细胞由视杆细胞、单锥细胞、双锥细胞组成,其中以视杆细胞占多数。视细胞与神经节细胞核比值平均为2.5:1,表明扬子鳄的组织结构与其弱光视觉相适应。     

The taxonomy and biology of further aeolidacean and arminacean nudibranch molluscs with symbiotic zooxanthellae

The occurrence of symbiotic zooxanthellae in further aeolid and arminacean nudibranch molluscs is described for the first time. The aeolid Aeolidiopsis ransoni Pruvot-Fol is redescribed, and a new species of Aeolidiopsis , also feeding on the colonial zoantharian Palythoa , is described. Both have symbiotic zooxanthellae. The taxonomy of the family Aeolidiidae is discussed and the genus Berghia Trinchese, 1877 is considered a synonym of Spurilla Bergh, 1864. Spurilla major (Eliot, 1903) and a new species of Spurilla are reported to have zooxanthellae while another new species of Spurilla is without zooxanthellae. The glaucid aeolid Pteraeolidia ianthina (Angas) is shown to have symbiotic zooxanthellae, as is the arminacean Doridomorpha gardineri Eliot, which is reported to feed on the alcyonarian blue coral, Heliopora. In all cases, the morphological adaptations developed for this symbiosis are described. Further notes on the Porites-fetding arminacean Pinufius rebus Marcus & Marcus and the aeolid Phestilla lugubris (Bergh) are included and a facultative symbiosis with zooxanthellae is suggested for the latter. The tion of symbiosis with zooxanthellae within nudibranchs is discussed and it is suggested that the relationship has evolved independently on several occasions.     

Carbohydrate metabolism in Musa paradisiaca

Considerable variations exist in the content of glucose, fructose, sucrose, starch and protein and in the activities of enzymes involved in carbohydrate metabolism between different parts of the banana plant (Musa paradisiaca). Sucrose synthetase is present in the highest concentration in rootstock and fruit pulp, and sucrose phosphate synthetase in the pseudostem. The highest ratio of the activity of sucrose phosphate synthetase to sucrose synthetase is found in leaves. Acid invertase is present in leaves, leaf-sheath and fruit pulp and is not demonstrable in rootstock and pseudostem. Neutral invertase activity is high in pseudostem and leaf-sheath. Starch phosphorylase is largely concentrated in fruit pulp and rootstock. The maximum activity of ATP:d-phosphoglucose (ADPG) pyrophosphorylase is found in rootstock. β-Amylase is not demonstrable in rootstock and is largely concentrated in leaf-sheath. Hexokinase is most active in rootstock and the lowest in leaves. Acid phosphatase and alkaline phosphatase activity is highest in fruit pulp and pseudostem. Glucosephosphate isomerase is most active in the rootstock and lowest in the leaves.