成骨蛋白-1的研究及应用进展

成骨蛋白-1（OP-1）是骨形态发生蛋白家族（BMPs）中的一员,具有较强的骨诱导活性,能够在体内、外诱导骨、软骨形成,完成骨修复能力。研究发现OP-1能治愈自体骨不能愈合的缺损,加速骨折愈合,能有效地促进横突体内融合发挥脊柱融合作用。OP-1用作盖髓剂,可以减轻炎症反应,诱导牙本质再生。此外,OP-1还具有抗肾脏纤维化作用,保护肾小球的完整性。     

成纤维细胞生长因子信号在骨损伤修复中的作用

骨损伤是常见的骨外科疾病。许多复杂的骨缺损,如创伤性大块骨缺损等常导致骨折延迟愈合及骨不连,是临床治疗中的难题。组织工程方法的运用为骨不连等的治疗提供了新的契机。成纤维细胞生长因子(fibroblast growth factor, FGF)信号在骨骼发育过程中发挥重要作用。基于其家族成员在骨折愈合过程中的时空表达及相关基因工程小鼠的表型,FGF信号相关分子被认为是骨再生修复的重要调节分子。该文将对FGF信号在骨损伤修复中的作用及应用方面的研究进展做综述,以期为其临床应用提供借鉴与参考。     

高效的骨诱导生长因子——成骨蛋白-1

成骨蛋白-1(OP-1)又称骨形态发生蛋白-7(BMP-7),属转化生长因子β(TGF-β)超家族成员.重组人OP-1(rhOP-1)在体内和体外都显示了高效的骨诱导活性,可使多种实验动物的骨缺损满意愈合,有良好的临床应用前景.     

人成骨蛋白-1成熟肽基因在大肠杆菌中的克隆与表达

本实验报告了人成骨蛋白 - 1成熟肽基因在大肠杆菌中的克隆与高效表达。通过计算机软件分析 ,采用大肠杆菌偏爱密码子设计并分段合成了人成骨蛋白 - 1成熟肽编码基因片段。用 PCR技术扩增目的基因片段 ,先克隆到 p UC1 9载体上 ,测序正确后再将其克隆到 p BV2 2 0表达载体上 ,以DH5α为宿主菌 ,42℃ ,6 h诱导表达。经 SDS- PAGE鉴定分析 ,在约 1 6× 1 0 3处有一新的蛋白质条带 ,扫描分析表明 ,该条带占菌体总蛋白含量的 40 %左右。     

高效的骨诱导生长因子——成骨蛋白-1

成骨蛋白１（ＯＰ１）又称骨形态发生蛋白７（ＢＭＰ７）,属转化生长因子β（ＴＧＦβ）超家族成员．重组人ＯＰ１（ｒｈＯＰ１）在体内和体外都显示了高效的骨诱导活性,可使多种实验动物的骨缺损满意愈合,有良好的临床应用前景     

成纤维细胞生长因子在骨修复中的作用和应用

成纤维细胞生长因子 ( FGF)是一类与肝素有高亲和性的多聚肽 ,最初根据它能刺激成纤维细胞再生而命名。现已发现有 1 8个成员 ,即 FGF1- FGF18。目前研究得最多的是 FGF1和 FGF2 。根据它们等电点 ( PI)的不同 ,FGF1又称为酸性成纤维细胞生长因子 ( a FGF,PI为 5 .6) ,FGF2 又称为碱性成纤维细胞生长因子 ( b FGF,PI为 9.6) ,它们均通过自分泌和 /或旁分泌途径在组织修复中发挥重要作用。成纤维细胞生长因子受体 ( FGFR)属于免疫球蛋白超家族成员 ,目前已确定了 4种由独立基因编码的人 FGFR。它们是一种跨膜蛋白质 ,分为细…     

下颌骨牵引成骨的研究进展和临床应用

牵引成骨是矫治骨骼畸形的一种新型技术,在颅颌面形整复外科领域中,以下颌骨牵引成骨方面的研究报道最多,本文就下颌骨牵引成骨的牵引原则,牵引装置,牵引生物力学及临床应用等内容作一简要综述。     

重组人成骨蛋白-1的离子交换及分子排阻色谱法纯化及其复性研究

经发酵大量表达重组人成骨蛋白-1(rhOP-1)。SDS-PAGE发现rhOP-1表达量占细菌总蛋白的35%。菌体经裂解、洗涤后,用8mol/L尿素溶解包涵体,离心后提取目的蛋白。经离子交换色谱法对变性状态下的目的蛋白进行纯化,绝大部分杂蛋白被去除,目的蛋白纯度达93%以上。为进一步提高目的蛋白浓度,采用分子排阻色谱法对目的蛋白进行再次纯化,纯度达98%以上。利用降低尿素梯度的方法对纯化的蛋白进行复性,二聚体的含量在50%以上。Westernblot证明了复性后的目的蛋白以单体和有活性的二聚体的形式存在。     

人成骨蛋白成熟肽基因在毕赤酵母中的克隆及其序列分析

采用PCR方法,根据献报道的人成骨蛋白-1（OP-1）成熟肽基因序列,设计并合一对引物,从含人成骨蛋白基因的质粒中扩增获得大小的420bp的DNA片段,连接到pGEM-T载体进行测序,证明获得人成骨蛋白成熟肽基因片段,继之以pPIC3.5k为表达载体构建重组表达质粒,并经PCR及酶切鉴定。     

浙江铁线莲属植物资源及其开发应用前景

浙江省铁线莲属植物作为重要的观赏植物和药用植物资源,其开发利用前景广阔。本文从植物分类学角度,总结了浙江省野生铁线莲属的种类及分布情况,并探讨其园林应用前景。     

Huntingtin-associated Protein-1 Interacts with Pro-brain-derived Neurotrophic Factor and Mediates Its Transport and Release

Brain-derived neurotrophic factor (BDNF) plays a pivotal role in brain development and synaptic plasticity. It is synthesized as a precursor (pro-BDNF), sorted into the secretory pathway, transported along dendrites and axons, and released in an activity-dependent manner. Mutant Huntingtin with expanded polyglutamine (polyQ) and the V66M polymorphism of BDNF reduce the dendritic distribution and axonal transport of BDNF. However, the mechanism underlying this defective transport remains unclear. Here, we report that Huntingtin-associated protein-1 (HAP1) interacts with the prodomain of BDNF and that the interaction was reduced in the presence of polyQ-expanded Huntingtin and BDNF V66M. Consistently, there was reduced coimmunoprecipitation of pro-BDNF with HAP1 in the brain homogenate of Huntington disease. Pro-BDNF distribution in the neuronal processes and its accumulation in the proximal and distal segments of crushed sciatic nerve and the activity-dependent release of pro-BDNF were abolished in HAP1^−/− mice. These results suggest that HAP1 may participate in axonal transport and activity-dependent release of pro-BDNF by interacting with the BDNF prodomain. Accordingly, the decreased interaction between HAP1 and pro-BDNF in Huntington disease may reduce the release and transport of BDNF.     

骨骼发育中的转录因子Cbfa1

骨骼由骨和软骨共同构成.最近的研究表明,转录因子Cbfa1不仅控制骨的形成和生长,还影响软骨组织成熟,并且可能与破骨细胞分化和软骨血管侵入有关.     

免疫检查点程序性细胞死亡蛋白配体-1翻译后修饰及其在免疫治疗中的应用前景

免疫检查点程序性细胞死亡蛋白配体-1(programmed cell death 1 ligand 1,PD-L1)是一种主要表达于肿瘤细胞表面的免疫抑制性分子,其可与T淋巴细胞表面的程序性细胞死亡蛋白-1(programmed cell death protein 1,PD-1)结合,抑制T淋巴细胞的激活,发挥免疫抑...     

粮果间作种植模式的资源利用与管理

粮果间作种植模式的资源利用与管理贺明荣,冷寿慈,李增嘉,张明亮（山东农业大学,泰安２７１０１８）ＲｅｓｏｕｒｃｅＵｔｉｌｉｚａｔｉｏｎａｎｄＩｔｓＭａｎａｇｅｍｅｎｔｉｎＧｒａｉｎａｎｄＦｒｕｉｔＩｎｔｅｒｃｒｏｐｐｉｎｇＳｙｓｔｅｍ．￥ＨｅＭｉｎｇ...     

Low-Density Lipoprotein Receptor-Related Protein-1 Mediates Endocytic Clearance of Tissue Inhibitor of Metalloproteinases-1 and Promotes Its Cytokine-Like Activities

Tissue inhibitor of metalloproteinases-1 (TIMP-1) regulates the extracellular matrix turnover by inhibiting the proteolytic activity of matrix metalloproteinases (MMPs). TIMP-1 also displays MMP-independent activities that influence the behavior of various cell types including neuronal plasticity, but the underlying molecular mechanisms remain mostly unknown. The trans-membrane receptor low-density lipoprotein receptor-related protein-1 (LRP-1) consists of a large extracellular chain with distinct ligand-binding domains that interact with numerous ligands including TIMP-2 and TIMP-3 and a short transmembrane chain with intracellular motifs that allow endocytosis and confer signaling properties to LRP-1. We addressed TIMP-1 interaction with recombinant ligand-binding domains of LRP-1 expressed by CHO cells for endocytosis study, or linked onto sensor chips for surface plasmon resonance analysis. Primary cortical neurons bound and internalized endogenous TIMP-1 through a mechanism mediated by LRP-1. This resulted in inhibition of neurite outgrowth and increased growth cone volume. Using a mutated inactive TIMP-1 variant we showed that TIMP-1 effect on neurone morphology was independent of its MMP inhibitory activity. We conclude that TIMP-1 is a new ligand of LRP-1 and we highlight a new example of its MMP-independent, cytokine-like functions.     

单核细胞趋化蛋白—1生物学特性及应用研究

趋化因子是不同类型细胞分泌的低分子量 (8～ 1 0ｋＤ)的细胞因子 ,它们对各种白细胞亚类如中性粒白细胞、单核细胞、淋巴细胞具有趋化作用。根据其 4个保守的半胱氨酸残基中前两个的位置 ,可将它们分成ＣＣ、ＣＸＣ、ＣＸ3Ｃ、Ｃ等 4个亚家族。趋化因子具有以下几个特点 :(1 )趋化因子在体外趋化一种或多种髓样细胞 ,(2 )脂多糖 (ＬＰＳ)、肿瘤坏死因子 (ＴＮＦ)、白介素 1 (ＩＬ 1 )、前炎症刺激物可导致大多数趋化因子的产生和分泌 ,(3 )动物皮内注射趋化因子均可引起炎症浸润[1] 。1 .单核细胞趋化蛋白 1 (ＭＣＰ 1 )生物学功能单核细胞…     

The Skeleton in the Physical Anthropology Closet1

Abstract

The “new” and “newer” concepts of physical anthropology are oriented to biochemical, genetic and evolutionary studies. While theseareneededand welcome concepts, they often slight the basic tools of anthropometry and typology. Also, the new and newer physical anthropology tends to separate biological factors from their cultural contexts. The writer discusses several questions posed by this shift in emphasis and suggests that the lack of a functional approach lessens the effectiveness of both biologically and culturally oriented investigators.