Al nanohole array plasmonic biosensors have been fabricated on polycarbonate (PC) substrates from conventional compact discs (CD). Standard micro and nanofabrication processes have been used and optimized to be PC compatible. The viability of this CD-based plasmonic platform for label-free optical biosensing has been demonstrated through a competitive bioassay for biotin analysis using biotin-functionalized dextran-lipase conjugates immobilized on the transducer surface. 相似文献
Optical transmission properties of periodic X-shaped plasmonic nanohole arrays in a silver film are investigated by performing the finite element method. Obvious peaks appear in the transmission spectra due to surface plasmon polaritons (SPPs) on the top surface of the silver film, to the Fabry–Ferot resonance effect of SPPs in the nanohole, and to the localized surface plasmon resonance of the nanohole. Besides the topologic shape parameters of the X-shaped nanohole, transmission properties strongly depend on incident polarization. The results of this study not only present a tunable plasmonic filter, but also aid in the understanding of the mechanisms of the extraordinary optical transmission phenomenon. 相似文献
Nanohole arrays in metal films allow extraordinary optical transmission (EOT); the phenomenon is highly advantageous for biosensing applications. In this article, we theoretically investigate the performance of refractive index sensors, utilizing square and hexagonal arrays of nanoholes, that can monitor the spectral position of EOT signals. We present near- and far-field characteristics of the aperture arrays and investigate the influence of geometrical device parameters in detail. We numerically compare the refractive index sensitivities of the two lattice geometries and show that the hexagonal array supports larger figure-of-merit values due to its sharper EOT response. Furthermore, the presence of a thin dielectric film that covers the gold surface and mimics a biomolecular layer causes larger spectral shifts within the EOT resonance for the hexagonal array. We also investigate the dependence of the transmission responses on hole radius and demonstrate that hexagonal lattice is highly promising for applications demanding strong light transmission. 相似文献
Plasmonics - The optical properties and the local electromagnetic field enhancement of a compound structure with rectangular nanohole arrays in Au-interlayer-Au multilayer films are numerically... 相似文献
A tunable high transmission optical bandpass filter based on a plasmonic hybrid nanostructure, composed of a periodic array of nanocircles and nanoholes combining two isolated waveguides is introduced in this paper. The presented design improves the coupling, which results in a higher transmission peak. To study the filtering operation, different topologies are investigated. The transmission properties and the resonance wavelengths are adjusted by sweeping various geometrical parameters. A multimode spectrum for each of the topologies is obtained. A tunable bandgap and bandwidth can be obtained by adjusting the refractive index of the periodic nanostructure. We have reached a maximum quality factor and a small full width at half-maximum bandwidth with the maximum transmission values greater than 80%. The advantages of the presented structures which include the benefits of both plasmonic and periodic nanostructures are tunability, high detection resolution, and integrability at the nanoscale for optical applications.
To enrich differentially expressed sequence tags (ESTs) for aluminum (A1) tolerance, cDNA subtraction libraries were generated from Al-stressed roots of two wheat (Triticum aestivum L.) nearisogenic lines (NILs) contrasting in Al-tolerance gene(s) from the Al-tolerant cultivar Atlas 66, using suppression subtractive hybridization (SSH). Expression patterns of the ESTs were investigated with nylon filter arrays containing 614 cDNA clones from the subtraction library. Gene expression profiles from macroarray analysis indicated that 25 ESTs were upregulated in the tolerant NIL in response to A1 stress. The result from Northern analysis of selected upregulated ESTs was similar to that from macroarray analysis. These highly expressed ESTs showed high homology with genes involved in signal transduction, oxidative stress alleviation, membrane structure, Mg^2 transportation, and other functions. Under A1 stress, the Al-tolerant NIL may possess altered structure or function of the cell wall, plasma membrane, and mitochondrion. The wheat response to A1 stress may involve complicated defense-related signaling and metabolic pathways. The present experiment did not detect any induced or activated genes involved in the synthesis of malate and other organic acids in wheat under Al-stress. 相似文献
To enrich differentially expressed sequence tags (ESTs) for aluminum (Al) tolerance, cDNA subtraction libraries were generated from Al-stressed roots of two wheat (Triticum aestivum L.) nearisogenic lines (NILs) contrasting in Al-tolerance gene(s) from the Al-tolerant cultivar Atlas 66, using suppression subtractive hybridization (SSH). Expression patterns of the ESTs were investigated with nylon filter arrays containing 614 cDNA clones from the subtraction library. Gene expression profiles from macroarray analysis indicated that 25 ESTs were upregulated in the tolerant NIL in response to Al stress. The result from Northern analysis of selected upregulated ESTs was similar to that from macroarray analysis. These highly expressed ESTs showed high homology with genes involved in signal transduction, oxidative stress alleviation, membrane structure, Mg2 transportation, and other functions. Under Al stress, the Al-tolerant NIL may possess altered structure or function of the cell wall, plasma membrane, and mitochondrion. The wheat response to Al stress may involve complicated defense-related signaling and metabolic pathways.The present experiment did not detect any induced or activated genes involved in the synthesis of malate and other organic acids in wheat under Al-stress. 相似文献
A facile strategy to deposit Pt nanoparticles with various metal‐loading densities on vertically aligned carbon nanotube (ACNT) arrays as electrocatalysts for proton exchange membrane (PEM) fuel cells is described. The deposition is achieved by electrostatic adsorption of the Pt precursor on the positively charged polyelectrolyte functionalized ACNT arrays and subsequent reduction by L ‐ascorbic acid. The application of the aligned electrocatalysts in fuel cells is realized by transferring from a quartz substrate to nafion membrane using a hot‐press procedure to fabricate the membrane electrode assembly (MEA). It is shown that the MEA with vertically aligned structured electrocatalysts provides better Pt utilization than that with Pt on conventional carbon nanotubes or carbon black, resulting in higher fuel cell performance. 相似文献
Standard slice electrophysiology has allowed researchers to probe individual components of neural circuitry by recording electrical responses of single cells in response to electrical or pharmacological manipulations1,2. With the invention of methods to optically control genetically targeted neurons (optogenetics), researchers now have an unprecedented level of control over specific groups of neurons in the standard slice preparation. In particular, photosensitive channelrhodopsin-2 (ChR2) allows researchers to activate neurons with light3,4. By combining careful calibration of LED-based photostimulation of ChR2 with standard slice electrophysiology, we are able to probe with greater detail the role of adult-born interneurons in the olfactory bulb, the first central relay of the olfactory system. Using viral expression of ChR2-YFP specifically in adult-born neurons, we can selectively control young adult-born neurons in a milieu of older and mature neurons. Our optical control uses a simple and inexpensive LED system, and we show how this system can be calibrated to understand how much light is needed to evoke spiking activity in single neurons. Hence, brief flashes of blue light can remotely control the firing pattern of ChR2-transduced newborn cells.Download video file.(48M, mov)相似文献
Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in biology and biotechnology are discussed. 相似文献
A new cloning vector, pMFY31, has been constructed based on the high-copy-number, broad-host-range plasmid RSF1010. The plasmid has a size of 13.2 kb and carries the Apr, Cmr, and Tcr genes. It contains unique PstI, EcoRI, HindIII, BamHI, and SalI sites, all of which are located within the antibiotic resistance genes, therefore all sites are applicable to insertional inactivation. We also constructed pMFY40, a 11.6 kb derivative of pMFY31, by the elimination of the Cmr gene. Plasmid pMFY31 has been efficiently introduced into a Pseudomonas putida strain not only by plasmid-DNA transformation but also by conjugal co-transfer with the helper plasmid, and was maintained stably in the strain. 相似文献
Microfabrication of dissolvable, swellable, and biodegradable polymeric microneedle arrays (MNs) were extensively investigated based in a nano sensitive fabrication style known as micromilling that is then combined with conventional micromolding technique. The aim of this study was to describe the polymer selection, and optimize formulation compounding parameters for various polymeric MNs. Inverse replication of micromilled master MNs reproduced with polydimethylsiloxane (PDMS), where solid out of plane polymeric MNs were subsequently assembled, and physicochemically characterized. Dissolvable, swellable, and biodegradable MNs were constructed to depth of less than 1 mm with an aspect ratio of 3.6, and 1/2 mm of both inter needle tip and base spacing. Micromolding step also enabled to replicate the MNs very precisely and accurate. Polymeric microneedles (MN) precision was ranging from ±0.18 to ±1.82% for microneedle height, ±0.45 to ±1.42% for base diameter, and ±0.22 to ±0.95% for interbase spacing. Although dissolvable sodium alginate MN showed less physical robustness than biodegradable polylactic-co-glycolic acid MN, their thermogravimetric analysis is of promise for constructing these polymeric types of matrix devices. 相似文献
Because of the scarcity of literature on the successful use of serological methods for differentiation of Rhizobium meliloti isolates, the objectives of this study were to provide a rationale for selecting isolates to which antisera could be raised and to appraise the suitability of published methods of preparing R. meliloti antigens for the serological identification of field isolates. We used one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis to develop protein profiles of eight field isolates and one commercial inoculant strain of R. meliloti in order to choose candidates that were either identical or distinctly different from each other for the production of antisera. The serological methods of tube agglutination and gel immunodiffusion complemented the sodium dodecyl sulfate-polyacrylamide gel electrophoresis method of identification. On the basis of their agglutination titers and gel immunodiffusion analysis, the isolates were placed in five serogroups which were identical to the groupings based on protein profiles. Antigenic characteristics of gel immunodiffusion antigens were influenced by the composition of the growth medium, sonication of whole-cell antigens, and the addition of Formalin. We recommend that careful attention be given to the effects of varying antigen preparation procedures when analyzing R. meliloti so that experimental protocols do not complicate the results. The wide range of homologous-antiserum titers observed for the nine isolates indicates different inherent degrees of immunogenicity of R. meliloti which cannot be predicted before serum production. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis method is a useful tool for screening a collection of R. meliloti isolates to better ensure that strain-specific antisera representative of different types of organisms will be obtained. 相似文献
We propose to achieve perfect trapping of light with asymmetric binary plasmon resonator arrays on metal substrates, in which antisymmetrically coupled resonance modes are excited in each subwavelength period to eliminate any leaky radiation. The specific structure in the study is an ultrathin binary metal stripe array on a flat metal substrate interspaced with a dielectric layer. The antiphase resonance modes are excited underneath the binary metal stripes in each period, resulting in perfect trapping of light under appropriate difference of the metal stripe widths. The trapped light is fully absorbed by metals, accompanied with an improved enhancement of the local field compared to those in symmetric structures with equal metal stripe widths. The work suggests a new way in designing optical metamaterials to manipulate light for enhanced light-matter interactions. 相似文献