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本文从血栓栓塞疾病的形成机制为依据,综述了纳豆激酶的药理作用。介绍了国内外产纤溶酶菌株的筛选方法,纳豆激酶的分离、纯化常用技术,纳豆激酶的生理生化特性、活性测定方法。并说明了影响纳豆激酶稳定的4个因素,和增加纳豆激酶产量的有效方法。通过药食两用纳豆激酶的研究进展,希望对纳豆激酶的研究提供理论基础。 相似文献
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纳豆激酶的分离纯化及生化研究 总被引:18,自引:0,他引:18
用固体发酵工艺制得的纳豆中含有大量溶栓活性成分———纳豆激酶。经过生理盐水浸提 ,(NH4 ) 2 SO4 分级沉淀 ,Sephadex柱层析等步骤 ,可以得一层析纯的纳豆激酶。冷冻干燥品经SDS—PAGE电泳显示有二组分存在 相似文献
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纳豆激酶的发酵工艺研究 总被引:26,自引:1,他引:26
以实验室选育的纳豆菌— 6号 (Bacillusnatto 6)为出发菌株 ,研究其固液两种发酵条件对纳豆激酶溶栓活力的影响。研究表明 ,纳豆菌适于固体发酵 ,37℃下 2 4h ,其活力可达 1 60 0IU/g以上。 相似文献
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纳豆激酶是从日本传统食品纳豆中提取的一种枯草杆菌蛋白酶,具有高效的抗血栓作用.与其他传统溶栓剂相比,纳豆激酶具有安全性好、成本低、易被人体吸收、作用直接迅速、作用持续时间长等优点,因此极有可能开发为新一代的溶栓剂.概括了纳豆激酶的分子结构、理化性质、溶栓作用机理、活性检测方法,介绍了其分子生物学研究进展,并对其开发应用进行了展望,证明纳豆激酶具有广阔的市场应用前景. 相似文献
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利用分光光度法对由纳豆菌发酵产生的纳豆激酶 (NK)进行了动力学性质的研究。以双倒数作图法 (L -B作图法 )求取Km。采用单因素试验法和正交试验法研究了底物浓度、酶浓度、温度、pH值对酶促反应速度的影响。结果表明该纳豆激酶的Km值为 3.4 98× 1 0 -6g·mL-1 ,当水解时间为 1 0min时 ,最适底物浓度为 1 6mg·mL-1 ,最适温度为 6 0℃ ,最适 pH为 8.0。 相似文献
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Béziat V Duffy D Quoc SN Le Garff-Tavernier M Decocq J Combadière B Debré P Vieillard V 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(12):6753-6761
Human NK cells comprise two main subsets, CD56(bright) and CD56(dim) cells, which differ in function, phenotype, and tissue localization. To further dissect the differentiation from CD56(bright) to CD56(dim) cells, we performed ex vivo and in vitro experiments demonstrating that the CD56(bright)CD16(+) cells are an intermediate stage of NK cell maturation. We observed that the maximal frequency of the CD56(bright)CD16(+) subset among NK cells, following unrelated cord blood transplantation, occurs later than this of the CD56(bright)CD16(-) subset. We next performed an extensive phenotypic and functional analysis of CD56(bright)CD16(+) cells in healthy donors, which displayed a phenotypic intermediary profile between CD56(bright)CD16(-) and CD56(dim)CD16(+) NK cells. We also demonstrated that CD56(bright)CD16(+) NK cells were fully able to kill target cells, both by Ab-dependent cell cytotoxicity (ADCC) and direct lysis, as compared with CD56(bright)CD16(-) cells. Importantly, in vitro differentiation experiments revealed that autologous T cells specifically encourage the differentiation from CD56(bright)CD16(-) to CD56(bright)CD16(+) cells. Finally, further investigations performed in elderly patients clearly showed that both CD56(bright)CD16(+) and CD56(dim)CD16(+) mature subsets were substantially increased in older individuals, whereas the CD56(bright)CD16(-) precursor subset was decreased. Altogether, these data provide evidence that the CD56(bright)CD16(+) NK cell subset is a functional intermediate between the CD56(bright) and CD56(dim) cells and is generated in the presence of autologous T CD3(+) cells. 相似文献
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Interaction between methemoglobin and cyanide was studied by low temperature inhibition method in combination with ESR. A new, not earlier described in literature ESR signal of low spin cyanide complex of this protein was recorded. 相似文献
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中国生物制品规程规定采用家兔试验法进行热原试验。规程要求当家兔降温≥06℃或2只及2只以上降温在04~06℃时应重试。在420批各类细胞因子热原质试验中发现23批出现降温,重试后合格率为100%。降温原因分析表明降温现象与热原性物质无关,与制品种类不存在显著相关性。因此,热原试验中出现家兔降温时是否需要重试值得商榷 相似文献
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